The hematopoietic regulator TAL1 is required for chromatin looping between the β-globin LCR and human γ-globin genes to activate transcription

Yun, Won Ju,Kim, Yea Woon,Kang, Yujin,Lee, Jungbae,Dean, Ann,Kim, AeRi Oxford University Press 2014 Nucleic acids research Vol.42 No.7

<P>TAL1 is a key hematopoietic transcription factor that binds to regulatory regions of a large cohort of erythroid genes as part of a complex with GATA-1, LMO2 and Ldb1. The complex mediates long-range interaction between the β-globin locus control region (LCR) and active globin genes, and although TAL1 is one of the two DNA-binding complex members, its role is unclear. To explore the role of TAL1 in transcription activation of the human γ-globin genes, we reduced the expression of TAL1 in erythroid K562 cells using lentiviral short hairpin RNA, compromising its association in the β-globin locus. In the TAL1 knockdown cells, the γ-globin transcription was reduced to 35% and chromatin looping of the <SUP>G</SUP>γ-globin gene with the LCR was disrupted with decreased occupancy of the complex member Ldb1 and LMO2 in the locus. However, GATA-1 binding, DNase I hypersensitive site formation and several histone modifications were largely maintained across the β-globin locus. In addition, overexpression of TAL1 increased the γ-globin transcription and increased interaction frequency between the <SUP>G</SUP>γ-globin gene and LCR. These results indicate that TAL1 plays a critical role in chromatin loop formation between the γ-globin genes and LCR, which is a critical step for the transcription of the γ-globin genes.</P>

Selected Tea and Tea Pomace Extracts Inhibit Intestinal α-Glucosidase Activity <i>in Vitro</i> and Postprandial Hyperglycemia <i>in Vivo</i>

Oh, Jungbae,Jo, Sung-Hoon,Kim, Justin S.,Ha, Kyoung-Soo,Lee, Jung-Yun,Choi, Hwang-Yong,Yu, Seok-Yeong,Kwon, Young-In,Kim, Young-Cheul MDPI 2015 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.16 No.4

<P>Type 2 diabetes mellitus (T2DM) is a metabolic disorder characterized by postprandial hyperglycemia, which is an early defect of T2DM and thus a primary target for anti-diabetic drugs. A therapeutic approach is to inhibit intestinal α-glucosidase, the key enzyme for dietary carbohydrate digestion, resulting in delayed rate of glucose absorption. Although tea extracts have been reported to have anti-diabetic effects, the potential bioactivity of tea pomace, the main bio waste of tea beverage processing, is largely unknown. We evaluated the anti-diabetic effects of three selected tea water extracts (TWE) and tea pomace extracts (TPE) by determining the relative potency of extracts on rat intestinal α-glucosidase activity <I>in vitro</I> as well as hypoglycemic effects <I>in vivo</I>. Green, oolong, and black tea bags were extracted in hot water and the remaining tea pomace were dried and further extracted in 70% ethanol. The extracts were determined for intestinal rat α-glucosidases activity, radical scavenging activity, and total phenolic content. The postprandial glucose-lowering effects of TWE and TPE of green and black tea were assessed in male Sprague-Dawley (SD) rats and compared to acarbose, a known pharmacological α-glucosidase inhibitor. The IC<SUB>50</SUB> values of all three tea extracts against mammalian α-glucosidase were lower or similar in TPE groups than those of TWE groups. TWE and TPE of green tea exhibited the highest inhibitory effects against α-glucosidase activity with the IC<SUB>50</SUB> of 2.04 ± 0.31 and 1.95 ± 0.37 mg/mL respectively. Among the specific enzymes tested, the IC<SUB>50</SUB> values for TWE (0.16 ± 0.01 mg/mL) and TPE (0.13 ± 0.01 mg/mL) of green tea against sucrase activity were the lowest compared to those on maltase and glucoamylase activities. In the animal study, the blood glucose level at 30 min after oral intake (0.5 g/kg body wt) of TPE and TWE of both green and black tea was significantly reduced compared to the control in sucrose-loaded SD rats. The TPE of all three teas had significantly higher phenolic content than those of the TWE groups, which correlated strongly with the DPPH radical scavenging activity. This is the first report of tea pomace extract significantly inhibits intestinal α-glucosidase, resulting in delayed glucose absorption and thereby suppressed postprandial hyperglycemia. Our data suggest that tea pomace-derived bioactives may have great potential for further development as nutraceutical products and the reuse of otherwise biowaste as valuable bioresources for the industry.</P>

Determination of Hg22+ Ions Using a Modified Glassy Carbon Electrode with 2,2’:6’:2”-Terpyridine

Young-TaeKong,Yun-JungBae,Yoon-BoShim 대한화학회 2002 Bulletin of the Korean Chemical Society Vol.23 No.2

Systematic Reliability Study of Top-Gate p- and n-Channel Organic Field-Effect Transistors

Hwang, Do Kyung,Fuentes-Hernandez, Canek,Fenoll, Mathieu,Yun, Minseong,Park, Jihoon,Shim, Jae Won,Knauer, Keith A.,Dindar, Amir,Kim, Hyungchul,Kim, Yongjin,Kim, Jungbae,Cheun, Hyeunseok,Payne, Marcia American Chemical Society 2014 ACS APPLIED MATERIALS & INTERFACES Vol.6 No.5

<P>We report on a systematic investigation on the performance and stability of p-channel and n-channel top-gate OFETs, with a CYTOP/Al<SUB>2</SUB>O<SUB>3</SUB> bilayer gate dielectric, exposed to controlled dry oxygen and humid atmospheres. Despite the severe conditions of environmental exposure, p-channel and n-channel top-gate OFETs show only minor changes of their performance parameters without undergoing irreversible damage. When correlated with the conditions of environmental exposure, these changes provide new insight into the possible physical mechanisms in the presence of oxygen and water. Photoexcited charge collection spectroscopy experiments provided further evidence of oxygen and water effects on OFETs. Top-gate OFETs also display outstanding durability, even when exposed to oxygen plasma and subsequent immersion in water or operated under aqueous media. These remarkable properties arise as a consequence of the use of relatively air stable organic semiconductors and proper engineering of the OFET structure.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/aamick/2014/aamick.2014.6.issue-5/am405424k/production/images/medium/am-2013-05424k_0007.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/am405424k'>ACS Electronic Supporting Info</A></P>

Neurologic outcomes of prehospital mechanical chest compression device use during transportation of out-of-hospital cardiac arrest patients: a multicenter observational study

Min Chanhong,Lee Dong Eun,Ryoo Hyun Wook,Jung Haewon,Cho Jae Wan,Kim Yun Jeong,Ahn Jae Yun,Park Jungbae,Mun You Ho,장태창,Jin Sang-Chan 대한응급의학회 2022 Clinical and Experimental Emergency Medicine Vol.9 No.3

Objective High-quality cardiopulmonary resuscitation with chest compression is important for good neurologic outcomes during out-of-hospital cardiac arrest (OHCA). Several types of mechanical chest compression devices have recently been implemented in Korean emergency medical services. This study aimed to identify the effect of prehospital mechanical chest compression device use on the outcomes of OHCA patients. Methods We retrospectively analyzed data drawn from the regional cardiac arrest registry in Daegu, Korea. This registry prospectively collected data from January 2017 to December 2020. Patients aged 18 years or older who experienced cardiac arrest presumed to have a medical etiology were included. The exposure variable was the use of a prehospital mechanical device during transportation by emergency medical technicians. The outcomes measured were neurologic outcomes and survival to discharge. Logistic regression analysis was used. Results Among 3,230 OHCA patients, 1,111 (34.4%) and 2,119 (65.6%) were managed with manual chest compression and with a mechanical chest compression device, respectively. The mechanical chest compression group showed poorer neurologic outcomes than the manual chest compression group (adjusted odds ratio, 0.12; 95% confidence interval, 0.04–0.33) and decreased survival to discharge (adjusted odds ratio, 0.39; 95% confidence interval, 0.19–0.82) after adjustment for confounding variables. Conclusion Prehospital mechanical chest compression device use in OHCA was associated with poorer neurologic outcomes and survival to discharge compared to manual chest compression.

지하철 기관사 휴게 공간 디자인을 위한 요구조사 분석

김국선(Kooksun Kim),김민지(Minji Kim),김중희(Joonghee Kim),송정효(Jeonghyo Song),신중배(Jungbae Shin),윤종현(Jonghyun Yun),최보아(Boah Choi) 한국가구학회 2015 한국가구학회지 Vol.26 No.1

There is an increasing trend each year in the number of subway users due to increase in the traffic volume. As the number of train service has risen, the subway drivers are given heavy work and from the same, are put at risk of mental health deterioration due to stress. As this is directly connected to safety, there is an urgent need for improvement of the situation. Therefore, as a way to appropriately relieve such stress, resting space where mental and physical energy can be recharged is necessary. Thus, this research conducted a survey of demand with subway drivers as subjects in order to provide resting space that can offer healthy rest and increase work effectiveness. 50 subway drivers were the subjects of this survey. As a result of analyzing the contents of the survey, environmental satisfaction was shown to be the lowest compared to psychological and morphological satisfaction. In the case of sleeping room, they wanted more comfortable and individual space. And the waiting room had the highest rate of usage frequency. The storage space was where the subway drivers showed the strongest attachment and was shown to be the only personal space. Most were shown to be satisfied with the outdoor space, but it had the lowest usage frequency. In addition, it is nessasery customized storage cabinet designed for subway drivers should be produced, which can effectively arrange the limited space. I hope that these direction of improvement can increase the healthy rest and work effectiveness of the locomotive engineers as it is directly being connected to the health and safety of the citizens.