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Nancy Dewi Yuliana,Mirna Zena Tuarita,Alfi Khatib,Farida Laila,Sukarno Sukarno 한국식품과학회 2020 Food Science and Biotechnology Vol.29 No.6
GC–MS metabolomics was used to discriminatethe phytochemicals profile of Indonesian white, red, andblack rice brans, and Japanese white rice brans. Thistechnique was used for the first time to identify compoundsin rice brans having cytotoxic activity against WiDr coloncancer cells. Orthogonal Projection to the Latent Structure(OPLS) analysis showed that protocatechuic acid (PA) wasa discriminating factor found in black rice brans whichstrongly correlated with its cytotoxicity (IC508.53 ± 0.26 lM). Real time-PCR data demonstrated thatPA cytotoxicity at different concentrations (1, 5, 10, 25 and50 lg/mL) was mediated through different pathways. Bcl-2 expression was downregulated at all tested concentrationsindicating apoptosis stimulation. At 1–10 ppm concentration,PA activated both intrinsic and extrinsicapoptosis pathways since the expression of p53, Bax,caspase-8, and caspase-9 were upregulated. At a higherdose (25 and 50 lg/mL), PA possibly involved in pyroptosis-mediated pro-inflammatory cell death by upregulatingthe expression of caspase-1 and caspase-7.
Nur Fathonah Sadek,Nancy Dewi Yuliana,Endang Prangdimurti,Bambang Pontjo Priosoeryanto,Slamet Budijanto 한국식품영양과학회 2017 Journal of medicinal food Vol.20 No.7
Plant sterols in their free forms are known to inhibit colon cancer. Whether these activities persist when compounds are incorporated into processed food is not reported yet. This study aimed to test the ability of plant sterol esters (PSE) incorporated into a nonpuffed extruded food (NPE) model to inhibit colon carcinogenesis. PSE was added into NPE at four concentrations (0.0%, 0.7%, 1.4%, and 2.1%). PSE-NPE activity was tested in azoxymethane/dextran sodium sulfate-induced Balb/c mice. The groups given PSE-NPE did not show any colon tumor formation. Immunohistochemistry results revealed that the group fed with 1.4% PSE had the lowest histoscore for cyclooxygenase-2 expression and the highest histoscore for cleaved caspase-3, cleaved caspase-8, and cleaved caspase-9expressions. The results of this study indicated that even after incorporation into a food system, which is processed using high pressure and temperature, PSE retained its chemopreventive activity. The proposed mechanisms are by suppressing inflammation and inducing apoptosis.
Windarsih Anjar,Bakar Nor Kartini Abu,Rohman Abdul,Yuliana Nancy Dewi,Dachriyanus Dachriyanus 아세아·태평양축산학회 2024 Animal Bioscience Vol.37 No.5
Objective: The adulteration of raw beef (BMr) with dog meat (DMr) and pork (PMr) becomes a serious problem because it is associated with halal status, quality, and safety of meats. This research aimed to develop an effective authentication method to detect non-halal meats (dog meat and pork) in beef using metabolomics approach. Methods: Liquid chromatography-high resolution mass spectrometry (LC-HRMS) using untargeted approach combined with chemometrics was applied for analysis non-halal meats in BMr. Results: The untargeted metabolomics approach successfully identified various metabolites in BMr DMr, PMr, and their mixtures. The discrimination and classification between authentic BMr and those adulterated with DMr and PMr were successfully determined using partial least square-discriminant analysis (PLS-DA) with high accuracy. All BMr samples containing non-halal meats could be differentiated from authentic BMr. A number of discriminating metabolites with potential as biomarkers to discriminate BMr in the mixtures with DMr and PMr could be identified from the analysis of variable importance for projection value. Partial least square (PLS) and orthogonal PLS (OPLS) regression using discriminating metabolites showed high accuracy (R2 >0.990) and high precision (both RMSEC and RMSEE <5%) in predicting the concentration of DMr and PMr present in beef indicating that the discriminating metabolites were good predictors. The developed untargeted LC-HRMS metabolomics and chemometrics successfully identified non-halal meats adulteration (DMr and PMr) in beef with high sensitivity up to 0.1% (w/w). Conclusion: A combination of LC-HRMS untargeted metabolomic and chemometrics promises to be an effective analytical technique for halal authenticity testing of meats. This method could be further standardized and proposed as a method for halal authentication of meats.