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- 저자 : Yuehui Ma (검색결과 4 건)
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Transcriptome Analysis of the Cytokinin Response in Medicago truncatula
Zhixiang Zhou,Haicong Liu,Cuina Ma,Yuehui Chao,Liebao Han 한국식물학회 2020 Journal of Plant Biology Vol.63 No.3
As an important legume plant, Medicago truncatula is a preeminent model for the study of the processes of nitrogen fxation, symbiosis, and legume genomics. The regulatory mechanism of the cytokinin response has been studied in many plants, such as rice, Arabidopsis, tomato, and barley, but information about regulatory pathways and genes involved in the cytokinin response in Medicago truncatula is notably limited. In this study, to better understand the cytokinin response in Medicago truncatula, transcriptome analysis of seedlings grown with 6-benzylaminopurine or lovastatin was performed using RNASeq. In this study, 3627 and 3093 transcripts were diferentially expressed in cytokinin-induced/control (Cyto/Ctrl) and cytokinin-inhibited/control (Inh/Ctrl) groups, respectively, and diferentially expressed genes were tested by quantitative real-time PCR (qRT-PCR). Analysis of the cytokinin response in Medicago truncatula revealed a large number of transcripts involved in signal transduction, metabolic process, secondary metabolite biosynthesis, transport and catabolism, growth and development, defense mechanisms, and transcription. There were 43 transcription factor families, including 1845 transcription factor (TF) genes with 2147 TF transcripts, as detected by RNA-Seq. Additionally, 216 TF genes with 220 transcripts were diferentially expressed in Cyto/Ctrl, and 185 TF genes with 189 transcripts were in Inh/Ctrl. A total of 289 and 260 DETs involved in biosynthesis, metabolism, and transduction of plant hormones were identifed in the Cyto/Ctrl and Inh/ Ctrl groups, respectively. Furthermore, 15 transcripts, including A-ARR, IPT, and CKX, were demonstrated to play roles in cytokinin regulation or signal transduction. These fndings were associated with the cytokinin response in other plants. The resulting data provide the frst cytokinin transcriptome analysis in Medicago truncatula. Further analysis and identifcation of cytokinin-regulated transcripts or signal transduction transcripts may help to elucidate the regulatory mechanisms governing the cytokinin response in Medicago truncatula.
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Rabiul Islam,Xuexue Liu,Gebremedhin Gebreselassie,Adam Abied,Qing Ma,Yuehui Ma 한국유전학회 2020 Genes & Genomics Vol.42 No.8
Background Litter size is the most important reproductive trait which plays a crucial role in goat production. Therefore, improvement of litter size trait has been of increasing interest in goat industry as small improvement in litter size may lead to large profit. The recent Cashmere goat breeding program produced a high-reproductive genetic line of Arbas Cashmere goat. But the genetic mechanism of high reproduction rate remains largely unknown in this Chinese native goat breed. To address this question, we performed a genome-wide association studies (GWAS) using two groups of goats varying in fecundity. Objectives Our study was aimed to investigate the significant SNPs and genes associated with high reproduction trait in Inner Mongolia Arbas Cashmere Goat. Methods We used logistic model association to perform GWAS using 47 goats from high fecundity group (~ 190%) and 314 goats from low fecundity group (~ 130%) of the Arbas Cashmere goat breed. Results We identified 66 genomic regions associated with genome wide significant level wherein six loci were found to be associated with reproduction traits. Further analysis showed that five key candidate genes including KISS1, KHDRBS2, WNT10B, SETDB2 and PPP3CA genes are involved in goat fecundity trait. Gene ontology enrichment analysis revealed that several biological pathways could be involved in the variation of fecundity in female goats. Conclusions The identified significant SNPs or genes provide useful information about the underlying genetic control of fecundity trait which will be helpful to use them in goat breeding programs for improving the reproductive efficiency of goats.
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Wang Jingfu,Wang Jingyi,Chang Xin,Shang Jin,Wang Yuehui,Ma Qin,Shen Liangliang 한국미생물·생명공학회 2023 Journal of microbiology and biotechnology Vol.33 No.8
Streptococcus mutans is the primary causative agent of caries, which is one of the most common human diseases. Thus, rapid and early detection of cariogenic bacteria is critical for its prevention. This study investigated the combination of loop-mediated isothermal amplification (LAMP) and microfluid technology to quantitatively detect S. mutans. A low-cost, rapid microfluidic chip using LAMP technology was developed to amplify and detect bacteria at 2.2–2.2 × 106 colony-forming units (CFU)/ml and its detection limits were compared to those of standard polymerase chain reaction. A visualization system was established to quantitatively determine the experimental results, and a functional relationship between the bacterial concentration and quantitative results was established. The detection limit of S. mutans using this microfluidic chip was 2.2 CFU/ml, which was lower than that of the standard approach. After quantification, the experimental results showed a good linear relationship with the concentration of S. mutans, thereby confirming the effectiveness and accuracy of the custom-made integrated LAMP microfluidic system for the detection of S. mutans. The microfluidic system described herein may represent a promising simple detection method for the specific and rapid testing of individuals at risk of caries.
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Embryoid bodies formation from chicken primordial germ cells
Hui Xiong,Yabin Pu,Qingyun Hu,Zhiqiang Shan,Pengfei Hu,Weijun Guan,Yuehui Ma 한국통합생물학회 2015 Animal cells and systems Vol.19 No.3
Primordial germ cells (PGCs) were demonstrated to be multipotential because of their differentiation ability in all embryonic lineages and because the pluripotential nature of PGCs caters for recent researches on stem cells. PGCs were cultured in suspension to form embryoid bodies (EBs). The characterization of PGCs and EBs was assessed by immunofluorescence technique, reverse transcription-polymerase chain reaction (RT-PCR) and paraffin section for Haematoxylin and Eosin (HE) stain. We established a stable chicken PGC line in vitro and prepared masses of EBs from PGCs. We also demonstrated that PGCs expressed stage-specific and stem cell-specific surface makers: SSEA-1, SSEA-3, Oct4, and Sox2. EBs expressed specific genes from three germ layers: gene AFP from entoderm, gene GATA6 from mesoderm, and gene Sox3 from ectoderm. The HE staining illustrated that EBs developed different cell types; relatively larger EBs (440 μm in diameter) were obtained, which contract rhythmically. We concluded that chicken PGCs were suitable for the formation of EBs. Abundant larger size EBs could be prepared in an effective way with our protocol, which could construct a good animal model and provide a useful clinical platform in studying human embryonic development and cell transplantation field.
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