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Yu Jung SOHN,Si Jae PARK 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
Practical use of biodegradable biopolymers has been an inevitable choice for the globe given the current environmental crisis including plastic pollution and climate change. Accordingly, it has become increasingly important to produce various polyhydroxyalkanoates (PHAs) since they are naturally occurring polyesters and exhibiting a variety of physical properties and thus material performances in accordance with the type of monomers employed and their compositions. To this end, a number of microorganisms including natural PHA-producer and nonnatural PHA-producer have been metabolically engineered for PHA production and among them, recombinant Escherichia coli has been the model microorganism capable of producing various PHAs. Thus, we devised 5-hydroxyvalerate (5HV)-containing PHAs production system in E. coli through a series of metabolic engineering strategies. Here, we will present the development of metabolically engineered E. coli strains for the production of PHAs consisting of 5HV as monomeric units.
Metabolic Engineering of Corynebacterium glutamicum for the Production of 5-Hydroxyvaleric Acid
Yu Jung SOHN,Minsoo KANG,Kei-Anne BARITUGO,Jina SON,Kyoung Hee KANG,Mi Hee RYU,Siseon LEE,Mingi SOHN,Ye Jean JUNG,Kyungmoon PARK,Si Jae PARK,Jeong Chan JOO,Hee Taek KIM 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.4
Regulation of lipid production by light-emitting diodes in human sebocytes.
Jung, Yu Ra,Kim, Sue Jeong,Sohn, Kyung Cheol,Lee, Young,Seo, Young Joon,Lee, Young Ho,Whang, Kyu Uang,Kim, Chang Deok,Lee, Jeung Hoon,Im, Myung Springer-Verlag 2015 Archives of dermatological research Vol.307 No.3
<P>Light-emitting diodes (LED) have been used to treat acne vulgaris. However, the efficacy of LED on sebaceous lipid production in vitro has not been examined. This study investigated the efficacy of 415 nm blue light and 630 nm red light on lipid production in human sebocytes. When applied to human primary sebocytes, 415 nm blue light suppressed cell proliferation. Based on a lipogenesis study using Oil Red O, Nile red staining, and thin-layered chromatography, 630 nm red light strongly downregulated lipid production in sebocytes. These results suggest that 415 nm blue light and 630 nm red light influence lipid production in human sebocytes and have beneficial effects on acne by suppressing sebum production.</P>
Yu, Su Jong,Kim, Hyunsoo,Min, Hophil,Sohn, Areum,Cho, Young Youn,Yoo, Jeong-Ju,Lee, Dong Hyeon,Cho, Eun Ju,Lee, Jeong-Hoon,Gim, Jungsoo,Park, Taesung,Kim, Yoon Jun,Kim, Chung Yong,Yoon, Jung-Hwan,Kim, American Chemical Society 2017 Journal of proteome research Vol.16 No.3
<P>This study was aimed to identify blood-based biomarkers to predict a sustained complete response (CR) after transarterial chemoembolization (TACE) using targeted proteomics. Consecutive patients with HCC who had undergone TACE were prospectively enrolled (training (n = 100) and validation set (n = 80)). Serum samples were obtained before and 6 months after TACE. Treatment responses were evaluated using the modified Response Evaluation Criteria in Solid Tumors (mRECIST). In the training set, the MRM-MS assay identified five marker candidate proteins (LRG1, APCS, BCHE, C7, and FCN3). When this five-marker panel was combined with the best-performing clinical variables (tumor number, baseline PIVKA, and baseline AFP), the resulting ensemble model had the highest area under the receiver operating curve (AUROC) value in predicting a sustained CR after TACE in the training and validation sets (0.881 and 0.813, respectively). Furthermore, the ensemble model was an independent predictor of rapid progression (hazard ratio (HR), 2.889; 95% confidence interval (CI), 1.612-5.178; P value < 0.001) and overall an unfavorable survival rate (HR, 1.985; 95% CI, 1.024-3.848; P value = 0.042) in the entire population by multivariate analysis. Targeted proteomics-based ensemble model can predict clinical outcomes after TACE. Therefore, this model can aid in determining the best candidates for TACE and the need for adjuvant therapy.</P>
Pharmacophore Identification for Peroxisome Proliferator-Activated Receptor Gamma Agonists
Sohn, Young-Sik,Lee, Yu-No,Park, Chan-In,Hwang, S-Wan,Kim, Song-Mi,Baek, A-Young,Son, Min-Ky,Suh, Jung-Keun,Kim, Hyong-Ha,Lee, Keun-Woo Korean Chemical Society 2011 Bulletin of the Korean Chemical Society Vol.32 No.1
Peroxisome proliferator-activated receptors (PPARs) are members of nuclear receptors and their activation induces regulation of fatty acid storage and glucose metabolism. Therefore, the $PPAR\gamma$ is a major target for the treatment of type 2 diabetes mellitus. In order to generate pharmacophore model, 1080 known agonists database was constructed and a training set was selected. The Hypo7, selected from 10 hypotheses, contains four features: three hydrogen-bond acceptors (HBA) and one general hydrophobic (HY). This pharmacophore model was validated by using 862 test set compounds with a correlation coefficient of 0.903 between actual and estimated activity. Secondly, CatScramble method was used to verify the model. Hence, the validated Hypo7 was utilized for searching new lead compounds over 238,819 and 54,620 chemical structures in NCI and Maybridge database, respectively. Then the leads were selected by screening based on the pharmacophore model, predictive activity, and Lipinski's rules. Candidates were obtained and subsequently the binding affinities to $PPAR\gamma$ were investigated by the molecular docking simulations. Finally the best two compounds were presented and would be useful to treat type 2 diabetes.
손현화,유기동,서영선,김태균,김양수,정재용,박유환,정춘해,홍승민,전제열,이상운 조선대학교 부설 의학연구소 1999 The Medical Journal of Chosun University Vol.24 No.2
Background: Cancer has become the major cause of death in Korea. Doctors who treat terminal cancer come across problems which are too difficult to solve. These problems are related with religious, moral and legal problems. These problems are easy death, patients' or family' s refuse of treatment, practice of cardiopulmonary resuscitation(CPR) in terminal cancer, definition of death and so forth. In Korea, studies related with terminal cancer patients and the doctors who treat them are very rare and the hospice system for terminal cancer patients is not organized yet. Objectives: The aim of this study was to identify the opinions of doctors who treat terminal cancer about these problems. Materials ðod: This study was performed on 90 doctors working at Chosun university hospital, who are divided in to three parts as staff, residents and interns. We used questionares related with the above problems, composed of 19 items and six general characteristics. We could confirm 75 questionaires. Results &onclusion: For the question "ve terminal cancer patients was announced their diagnosis and prognosis?"hat was most important to doctors was patients' opinions and the second was their family' s. For the question "eatment which could prolong the life of terminal cancer patients who are unnconscious is performed?"doctors showed a negative opinion. About the easy death of terminal cancer patients, more than half the doctors showed agreement. The prohibition of CPR in terminal cancer patients was agreed by 40%. For the question about the definition of death in terminal cancer patients 76.1% doctors agreed it was brain death. 85.7% subjects answered that they felt moral enmity in treatment of terminal cancer patients. In Korea, the programs for these problems are very poor, and the results of this study can affect the formation of systems and laws about the terminal cancer patients and the doctor's treatment. National support for terminal cancer patients and the hospice care system is required.