Photochemical Response Analysis on Drought Stress for Red Pepper (Capsiumannuum L.)

Sung-Yung Yoo,Yong-Ho Lee,So-Hyun Park,Kyong-mi Choi,June-Young Park,A-Ram Kim,Su-Min Hwang,Min-Ju Lee,Tae-Seok Ko,Tae-Wan Kim 한국토양비료학회 2013 한국토양비료학회지 Vol.46 No.6

The aim of this study is to determine the drought stress index through photochemical analysis in red pepper (Capsiumannuum L.). The photochemical interpretation was performed in the basis of the relation between Kautsky effect and Photosystem II (PSII) following the measurement of chlorophyll, pheophytin contents, and CO₂ assimilation in drought stressed 5-week-old red pepper plants. The CO₂ assimilation rate was severely lowered with almost 77% reduction of chlorophyll and pheophytin contents at four days after non-irrigation. It was clearly observed that the chlorophyll fluorescence intensity rose from a minimum level (the O level), in less than one second, to a maximum level (the P-level) via two intermediate steps labeled J and I (OJIP process). Drought factor index (DFI) was also calculated using measured OJIP parameters. The DFI was -0.22, meaning not only the initial inhibition of PSII but also sequential inhibition of PSI . In real, most of all photochemical parameters such as quantum yield of the electron transport flux from Quinone A (QA) to Quinone B (QB), quantum yield of the electron transport flux until the PSI electron acceptors, quantum yield of the electron transport flux until the PSI electron acceptors, average absorbed photon flux per PSII reaction center, and electron transport flux until PSI acceptors per cross section were profoundly reduced except number of QA reducing reaction centers (RCs) per PSII antenna chlorophyll (RC/ABS). It was illuminated that at least 6 parameters related with quantum yield/efficiency and specific energy fluxes (per active PSII RC) could be applied to be used as the drought stress index. Furthermore, in the combination of parameters, driving forces (DF) for photochemical activity could be deduced from the performance index (PI) for energy conservation from photons absorbed by PSII antenna until the reduction of PSI acceptors. In conclusion, photochemical responses and their related parameters can be used as physiological DFI.

Role of Glutathione Conjugation in 1-Bromobutane-induced Immunotoxicity in Mice

Sang Kyu Lee,Dong Ju Lee,Tae Won Jeon,Gyu Sub Ko,Se Hyun Yoo,Hyun Woo Ha,Mi Jeong Kang,Wonku Kang,Sang Kyum Kim,Tae Cheon Jeong 한국독성학회 2010 Toxicological Research Vol.26 No.2

Halogenated organic compounds, such as 1-bromobutane (1-BB), have been used as cleaning agents, agents for chemical syntheses or extraction solvents in workplace. In the present study, immunotoxic effects of 1-BB and its conjugation with glutathione (GSH) were investigated in female BALB/c mice. Animals were treated orally with 1-BB at 375, 750 and 1500 ㎎/㎏ in corn oil once for dose response or treated orally with 1-BB at 1500 ㎎/㎏ for 6, 12, 24 and 48 hr for time course. S-Butyl GSH was identified in spleen by liquid chromatography-electrospray ionization tandem mass spectrometry. Splenic GSH levels were significantly reduced by single treatment with 1-BB. S-Butyl GSH conjugates were detected in spleen from 6 hr after treatment. Oral 1-BB significantly suppressed the antibody response to a T-dependent antigen and the production of splenic intracellular interlukin-2 in response to Con A. Our present results suggest that 1-BB could cause immunotoxicity as well as reduction of splenic GSH content, due to the formation of GSH conjugates in mice. The present results would be useful to understand molecular toxic mechanism of low molecular weight haloalkanes and to develop biological markers for exposure to haloalkanes.

External Application of Fermented Olive Flounder (Paralichthys olivaceus) Oil Alleviates Inflammatory Responses in 2,4-Dinitrochlorobenzeneinduced Atopic Dermatitis Mouse Model

Sang-Chul Han,Gyeoung-Jin Kang,Yeong-Jong Ko,Hee-Kyoung Kang,Sang-Wook Moon,Yong-Seok Ann,Eun-Sook Yoo 한국독성학회 2012 Toxicological Research Vol.28 No.3

Allergic skin inflammation such as atopic dermatitis (AD) is characterized by edema and infiltration with various inflammatory cells such as mast cells, basophils, eosinophils and T cells. Thymic stromal lymphopoietin (TSLP) is produced mainly by epidermal keratinocytes, as well as dermal fibroblasts and mast cells in the skin lesions of AD. Omega-3 polyunsaturated fatty acids in fish oil can reduce inflammation in allergic patients. Fermentation has a tremendous capacity to transform chemical structures. The antiinflammatory effects of fish oil have been described in many diseases, but the beneficial effects by which fermented olive flounder oil (FOF) modulates the allergic response is poorly understood. In this study, we produced FOF and tested its ability to suppress the various allergic inflammatory responses. The ability of FOF to modulate the immune system was investigated using a mouse model of AD. The FOF-treated group showed significantly decreased immunoglobulin E (IgE) and histamine in serum. Also, the increased TSLP expression was significantly inhibited in the FOF group; the FOF-treated group was not appreciably different from the hydrocort cream treatment group. In addition, FOF treatment resulted in a smaller spleen size with reduced the thickness and length compared to the induction group. Splenocytes from mice treated with FOF produced significantly less IFN-γ, IL-4, T-box transcription factor (T-bet) and GATA binding protein 3 (GATA3) expression compared with the induction group. These results suggest that FOF may be effective in treating the allergic symptoms of AD. 5.

Concomitant moyamoya syndrome and infratentorial arteriovenous malformation in a neurofibromatosis type 1 patient: a case report

Ko Eunbuel,Hwang Il-Jung,Kim Jung-Woo,Lee Ju-Suck,Jung Dar-Eun,Yoo Sang-Won 대한신경집중치료학회 2023 대한신경집중치료학회지 Vol.16 No.1

Background: Neurofibromatosis type 1 (NF1) is a genetic disorder with diverse phenotypic manifestations. Cerebral vasculopathy is one of the multisystem involvements often overlooked unless symptomatic. Case Report: A 28-year-old male patient with prolonged NF1 complained of right-hand position-specific rhythmic tremor after surviving an ipsilateral cerebellar arteriovenous malformation (AVM) hemorrhagic transformation. Not only did he suffer rupture of the infratentorial vasculopathy but he also endured asymmetric supratentorial occlusive vessel changes in Moyamoya syndrome. Due to contralateral limb clumsiness, his right hemispheric vasculature was revascularized by encephaloduroarteriosynangiosis 13 years before the AVM rupture.Conclusion: This case report describes exceptional NF1 CNS involvement where the cerebral vasculature had concomitant Moyamoya syndrome and unilateral cerebellar AVM in a single patient. Cerebral vasculopathy should be surveyed and adequately addressed during the follow-up of chronic NF1, as it can cause irreversible sequelae or can be life-threatening.

Solution deposition planarization for IBAD-MgO texture template

Ko, Kyeong-Eun,Kwon, O-Jong,Bea, Sung-Hwan,Yoo, Ja-Eun,Park, Chan,Oh, Sang-Soo,Park, Young-Kuk The Korean Society of Superconductivity and Cryoge 2010 한국초전도저온공학회논문지 Vol.12 No.4

In this work, the optimized process condition of chemical solution deposition which is used to planarize the surface of the metal tape (which is used to grow IBAD-MgO texture template) was investigated. $Y_2O_3$ films were dip-coated on the surface of the unpolished metal tape as the seed and barrier layer. The effects of $Y_2O_3$ concentration of the solution (0.5wt.%, 1.3wt.%, 2.8wt.%, 5.6wt.%) and the number of coatings on the surface morphology and barrier capability against the diffusion from the metal tape were examined. The surface morphology and the thickness of the film were observed using the scanning electron microscope and the atomic force microscope. The presence of elements in metal tape on the film surface was analyzed using the auger electron spectroscopy. The $Y_2O_3$ film thickness increases with increasing the $Y_2O_3$ concentration in the solution, and the surface became smoother with increasing the number of coating cycles. The best result was obtained from the $Y_2O_3$ film coated 4 cycles using 2.8wt.% solution.

Fabrication of fine and homogeneous MgB₂ nano powders by spray pyrolysis

Ko, Jae-Woong,Yoo, Jaimoo,Kim, Young-Kuk,Chung, Kook-Chae,Chung, Hyungsik,Yoo, Sang-Im Institute of Electrical and Electronics Engineers 2005 IEEE transactions on applied superconductivity Vol.15 No.2

Homogeneous nano-sized spherical MgB<SUB>2</SUB> powders were fabricated by spray pyrolysis. The influence of solution concentration, furnace reaction temperature and mass flow of carrier gas on morphology and average particle size were investigated. MgB<SUB>2</SUB> powders were synthesized with or without further heat treatment. For adequate preparation conditions, it has mostly spherical, solid and narrow particle size distribution. Average particle size (X<SUB>50</SUB>) distribution was below 100 nm. The critical temperature for the synthesized MgB<SUB>2</SUB> was around 36 K.

A relationship between Stroke and Sasang Constitution in Korean

Ko, Ho-Yeon,Jun, Chan-Yong,Park, Jong-Hyeong,Yoon, Yoo-Sik,Lee, Sun-Dong,Han, Chang-Ho,Jung, Woo-Sang,Moon, Sang-Kwan,Cho, Ki-Ho,Ko, Seong-Gyu Kyung Hee Oriental Medicine Research Center 2005 Oriental pharmacy and experimental medicine Vol.5 No.4

Experts of Sasang Constitution Medicine of Traditional Korean Medicine have classified stroke patients with four types of Sasang constitutions in their clinical practice and some types of Sasang constitutions have been regarded as risk factors of stroke, but this is uncertain because there were no evidences by large scale of prospective studies. The purpose of this was to study the association between strokes and Sasang constitutions. Case-control study has been conducted to the patients admitted to the research hospitals. The patients were confirmed stroke by brain MRI or CT scans and recruited from May 2003 to August 2005. The subjects who met the requirement of inclusion and exclusion criteria were 108 patients as the cases and 107 as healthy controls. Data collection has been performed by the trained specialists majoring neurologists through interviews, physical examinations, and laboratory testes. No statistical significance was obtained between the strokes and Sasang constitutions, yet Taeumin, and Soyangin types showed a trend of increase in the incidence of strokes as compared with Soeumin. To acquire more concrete data on this theme, we need further and large scale of prospective researches.

The lived experience of ethical dilemma of ICU nurses

Sung-Hee Ko(고성희),Seon-Hee Yoo(유선희),Young-Ju Yun(윤영주),Sung-Hee Lee(이성희) 질적연구학회 2006 질적연구학회 학술대회 자료집 Vol.2006 No.-

Intratumoral distribution of ⁶⁴Cu-ATSM and ¹⁸F-FDG in VX2tumor xenografted rabbit

Yoo, Ran Ji,Lee, Ji Woong,Lee, Kyo Chul,An, Gwang Il,Ko, In Ok,Chung, Wee Sup,Park, Ji Ae,Kim, Kyeong Min,Choi, Yang-Kyu,Kang, Joo Hyun,Lim, Sang Moo,Lee, Yong Jin 대한방사성의약품학회 2015 Journal of radiopharmaceuticals and molecular prob Vol.1 No.2

$^{64}Cu$-labeled diacetyl-bis($N^4$-methylthiosemicarbazone) is a promising agent for internal radiation therapy and imaging of hypoxic tissues. In the study, we confirmed hypoxia regions in VX2 tumor implanted rabbits with injection $^{64}Cu$-ATSM and $^{18}F$-FDG using positron emission tomography (PET)/computed tomography (CT). PET images with $^{18}F$-FDG and $^{64}Cu$-ATSM were obtained for 40 min by dynamic scan and additional delayed PET images of $^{64}Cu$-ATSM the acquired up to 48 hours. Correlation between intratumoral $O_2$ level and $^{64}Cu$-ATSM PET image was analyzed. $^{64}Cu$-ATSM and $^{18}F$-FDG were intravenously co-injected and the tumor was dissected and cut into slices for a dual-tracer autoradiographic analysis. In the PET imaging, $^{64}Cu$-ATSM in VX2 tumors displayed a specific uptake in hypoxic region for48 h. The uptake pattern of $^{64}Cu$-ATSM in VX2 tumor at 24 and 48 h did not match to the $^{18}F$-FDG. Through ROI analysis, in the early phase (dynamic scan), $^{18}F$-FDG has positive correlation with $^{64}Cu$-ATSM but late phase (24 and 48 h) of the $^{64}Cu$-ATSM showed negative correlation with $^{18}F$-FDG. High uptake of $^{64}Cu$-ATSM in hypoxic region was responded with significant decrease of oxygen pressure, which confirmed by $^{64}Cu$-ATSM PET imaging and autoradiographic analysis. In conclusion, $^{64}Cu$-ATSM can utilize for specific targeting of hypoxic region in tumor, and discrimination between necrotic- and viable hypoxic tissue.

Research Trends in Pharmacopuncture Treatment for Lateral Epicondylitis

Yoo, Jae Hee,Ko, Hong Je,Jeong, Sang Jun,Kim, Min Wook,Kim, Soo Hyun,Kim, Seon Woo,Jang, Seung Won,Shin, Jeong Cheol Korean AcupunctureMoxibustion Medicine Society 2020 대한침구의학회지 Vol.30 No.1

The aim of this study was to examine pharmacopuncture treatment for lateral epicondylitis, and to contribute to developing a standardized treatment regimen by reviewing trends in clinical trials. Five randomized controlled trials, 1 case-control study, and 8 cohort studies published after 1999, that involved pharmacopuncture for lateral epicondylitis, were selected from Korean and international online databases (n = 8). The type of pharmacopuncture, dose, frequency, efficacy, and adverse events were analyzed. Seven types of pharmacopuncture were used, namely Bee Venom, Illicium henryi Diels, Akebiae Caulis, Angelicae sinensis Diels, Ligusticum chuanxiong Hort, Hominis Placenta, and Salviae Miltiorrhizae Radix. Dose, treatment duration, and treatment frequency varied widely. One study assessed the treatment efficacy according to frequency. Nine studies lacked data on adverse events. The quality of 5 randomized controlled trials was low. Although pharmacopuncture treatment appeared to be effective for lateral epicondylitis, it was difficult to standardize the regimen for lateral epicondylitis.