ATP-dependent chromatin remodeler CHD9 controls the proliferation of embryonic stem cells in a cell culture conditiondependent manner

Hyunjin Yoo,Kwonho Hong 한국실험동물학회 2021 한국실험동물학회 학술발표대회 논문집 Vol.2021 No.1

An Optimized Method for the Construction of a DNA Methylome from Small Quantities of Tissue or Purified DNA from Arabidopsis Embryo

Yoo, Hyunjin,Park, Kyunghyuk,Lee, Jaehoon,Lee, Seunga,Choi, Yeonhee Korean Society for Molecular and Cellular Biology 2021 Molecules and cells Vol.44 No.8

DNA methylation is an important epigenetic mechanism affecting genome structure, gene regulation, and the silencing of transposable elements. Cell- and tissue-specific methylation patterns are critical for differentiation and development in eukaryotes. Dynamic spatiotemporal methylation data in these cells or tissues is, therefore, of great interest. However, the construction of bisulfite sequencing libraries can be challenging if the starting material is limited or the genome size is small, such as in Arabidopsis. Here, we describe detailed methods for the purification of Arabidopsis embryos at all stages, and the construction of comprehensive bisulfite libraries from small quantities of input. We constructed bisulfite libraries by releasing embryos from intact seeds, using a different approach for each developmental stage, and manually picking single-embryo with microcapillaries. From these libraries, reliable Arabidopsis methylome data were collected allowing, on average, 11-fold coverage of the genome using as few as five globular, heart, and torpedo embryos as raw input material without the need for DNA purification step. On the other hand, purified DNA from as few as eight bending torpedo embryos or a single mature embryo is sufficient for library construction when RNase A is treated before DNA extraction. This method can be broadly applied to cells from different tissues or cells from other model organisms. Methylome construction can be achieved using a minimal amount of input material using our method; thereby, it has the potential to increase our understanding of dynamic spatiotemporal methylation patterns in model organisms.

Conjugation of vascular endothelial growth factor to poly lactic-co-glycolic acid nanospheres enhances differentiation of embryonic stem cells to lymphatic endothelial cells

Yoo Hyunjin,Choi Dongyoon,Choi Youngsok 아세아·태평양축산학회 2021 Animal Bioscience Vol.34 No.4

Objective: Pluripotent stem cell-derived lymphatic endothelial cells (LECs) show great promise in their therapeutic application in the field of regenerative medicine related to lymphatic vessels. We tested the approach of forced differentiation of mouse embryonal stem cells into LECs using biodegradable poly lactic-co-glycolic acid (PLGA) nanospheres in conjugation with growth factors (vascular endothelial growth factors [VEGF-A and VEGF-C]). Methods: We evaluated the practical use of heparin-conjugated PLGA nanoparticles (molecular weight ~15,000) in conjugation with VEGF-A/C, embryoid body (EB) formation, and LEC differentiation using immunofluorescence staining followed by quantification and quantitative real-time polymerase chain reaction analysis. Results: We showed that formation and differentiation of EB with VEGF-A/C-conjugated PLGA nanospheres, compared to direct supplementation of VEGF-A/C to the EB differentiation media, greatly improved yield of LYVE1(+) LECs. Our analyses revealed that the enhanced potential of LEC differentiation using VEGF-A/C-conjugated PLGA nanospheres was mediated by elevation of expression of the genes that are important for lymphatic vessel formation. Conclusion: Together, we not only established an improved protocol for LEC differentiation using PLGA nanospheres but also provided a platform technology for the mechanistic study of LEC development in mammals. Objective: Pluripotent stem cell-derived lymphatic endothelial cells (LECs) show great promise in their therapeutic application in the field of regenerative medicine related to lymphatic vessels. We tested the approach of forced differentiation of mouse embryonal stem cells into LECs using biodegradable poly lactic-co-glycolic acid (PLGA) nanospheres in conjugation with growth factors (vascular endothelial growth factors [VEGF-A and VEGF-C]).Methods: We evaluated the practical use of heparin-conjugated PLGA nanoparticles (molecular weight ~15,000) in conjugation with VEGF-A/C, embryoid body (EB) formation, and LEC differentiation using immunofluorescence staining followed by quantification and quantitative real-time polymerase chain reaction analysis.Results: We showed that formation and differentiation of EB with VEGF-A/C-conjugated PLGA nanospheres, compared to direct supplementation of VEGF-A/C to the EB differentiation media, greatly improved yield of LYVE1(+) LECs. Our analyses revealed that the enhanced potential of LEC differentiation using VEGF-A/C-conjugated PLGA nanospheres was mediated by elevation of expression of the genes that are important for lymphatic vessel formation.Conclusion: Together, we not only established an improved protocol for LEC differentiation using PLGA nanospheres but also provided a platform technology for the mechanistic study of LEC development in mammals.

Color Calibration of HDR Image under a Known Illumination for Measuring Reflectance Property of Materials

YOO, Hyunjin,KIM, Kang Y.,LEE, Kwan H. The Institute of Electronics, Information and Comm 2009 IEICE transactions on information and systems Vol.92 No.12

<P>High Dynamic Range Imaging (HDRI) refers to a set of techniques that can represent a dynamic range of real world luminance. Hence, the HDR image can be used to measure the reflectance property of materials. In order to reproduce the original color of materials using this HDR image, characterization of HDR imaging is needed. In this study, we propose a new HDRI characterization method under a known illumination condition at the HDR level. The proposed method normalizes the HDR image by using the HDR image of a light and balances the tone using the reference of the color chart. We demonstrate that our method outperforms the previous method at the LDR level by the average color difference and BRDF rendering result. The proposed method gives a much better reproduction of the original color of a given material.</P>

Mouse Dot1L controls development of lymphatic vessels

Hyunjin Yoo,Kwonho Hong 한국실험동물학회 2021 한국실험동물학회 학술발표대회 논문집 Vol.2021 No.7

Proper functioning of the lymphatic system is required for normal immune responses, fluid balance, and lipid reabsorption. Multiple regulatory mechanisms are employed to ensure the correct formation and function of lymphatic vessels; however, the epigenetic modulators and mechanisms involved in this process are poorly understood. Here, we assess the regulatory role of mouse Dot1l, a histone H3 lysine (K) 79 (H3K79) methyltransferase, in lymphatic formation. Genetic ablation of Dot1l in Tie2(+) endothelial cells (ECs), but not in Lyve1(+) or Prox1(+) lymphatic endothelial cells (LECs) or Vav1(+) definitive hematopoietic stem cells, leads to catastrophic lymphatic anomalies, including skin edema, blood–lymphatic mixing, and underdeveloped lymphatic valves and vessels in multiple organs. Remarkably, targeted Dot1l loss in Tie2(+) ECs leads to fully penetrant lymphatic aplasia, whereas Dot1l overexpression in the same cells results in partially hyperplastic lymphatics in the mesentery. Genetic studies reveal that Dot1l functions in c-Kit(+) hemogenic ECs during mesenteric lymphatic formation. Mechanistically, inactivation of Dot1l causes a reduction of both H3K79me2 levels and the expression of genes important for LEC development and function. Thus, our study establishes that Dot1l-mediated epigenetic priming and transcriptional regulation in LEC progenitors safeguard the proper lymphatic development and functioning of lymphatic vessels.

스마트워치 알림에 대한 감각적 인지 가능성 연구

유현진(Hyunjin Yoo) 한국HCI학회 2016 한국HCI학회 학술대회 Vol.2016 No.1

웨어러블 디바이스를 활용한 운동 중 피드백 방식 연구

유현진(Hyunjin Yoo),맹욱재(Wookjae Maeng),이중식(Joongseek Lee) 한국HCI학회 2016 한국HCI학회 논문지 Vol.11 No.3

현재 피트니스 트래커(fitness tracker)시장은 유산소 운동에만 초점을 맞춰 근력 운동 분야는 상대적으로 소외되어 있다. 최근 근력 운동 피트니스 트래커가 소수 출시되고 있으나, 운동 상황에 대한 고려가 부족하여 사람-기기 간 인터랙션에 불편함을 초래한다. 특히, 운동 중에는 신체의 움직임이 활발하므로, 손으로 기기를 휴대하거나 기기를 조작하는 상황은 사용자에게 부정적 경험을 유발한다. 웨어러블 디바이스는 항시적인 착용이 가능하므로, 손과 발의 사용이 자유로워 운동 중 피드백 제공에 효과적이다. 따라서, 이 연구에서는 웨어러블 디바이스를 통해 피드백을 감각하게 함으로써, 운동 수행자가 효과적인 운동을 할 수 있도록 하는 것을 목적으로 한다. 이 연구에서는 다음 세 가지 연구 문제를 검증하였다. 1) 운동 상황에서 필요한 정보는 무엇인가? 2) 근력 운동 중, 어떤 감각 피드백이 선호되는가? 3) 근력 운동 중, 감각 피드백의 가치는 무엇인가? 그 결과, 첫째, 운동 수행자는 운동 단계 중, ‘운동 중’에 해당하는 정보가 가장 필요하다고 하였으며 ‘페이스 조절(횟수 카운팅, 동기 부여)’과 ‘자세 지도(문제점 진단, 자세 교정)"에 해당하는 정보를 가장 필요로 하였다. 둘째, 운동 중 선호되는 감각 피드백에 대해서는 청각 피드백, 촉각 피드백, 시각 피드백 순으로 만족도가 높았으며, 운동 강도가 높을수록 감각 피드백에 대한 만족도가 더 높았다. 셋째, 감각 피드백과 기기 피드백의 가치에 대해 비교한 결과, 기기 피드백과 사람이 제공하는 피드백에 대한 만족도, 유용성, 효용성이 비슷하게 나타났다. 결론적으로, 이 연구에서는 근력 운동 중, 웨어러블 디바이스를 활용한 감각 피드백의 디자인 가이드라인을 도출하였으며, 웨어러블 디바이스의 감각 피드백이 사람이 제공하는 피드백을 대체할 수 있다는 가능성을 확인하였다. The landscape of the current fitness trackers is not only limited to the aerobic exercises but also the weight training is comparatively excluded. Recently, a few weight training fitness tracker was released, human-computer interaction was not well designed due to the lack of considering the context. Because body movement would be intense while doing exercises, having exercise performers hold or operate a device makes a negative experience. As the wearable device is always inseparable to body, it could provide effective feedback because holding or operating a device is not necessary. Therefore, this study aims to make the exercise performers feel a natural feedback through the wearable device to do effective exercises. As a result, this study identified three findings. First, the information which exercise performers most needed was ‘during exercise.’ and the most necessary information for exercise performers through wearable device’s sensory feedback was about ‘pace control’ with counting and motivation. Second, the order of the most preferred presentation type of sensory feedback was auditory feedback, haptic feedback and visual feedback. Third, the satisfaction, utility, usefulness score of sensory feedback as same as the personal trainer’s feedback. In conclusion, this study illustrated the feedback design implications using a wearable device while doing weight training and the possibilities that wearable device could be substitute for personal trainer.

다중 스펙트럼 영상 시스템 기반의 스펙트럼 반사율 추정

유현진 ( Hyunjin Yoo ),김덕봉 ( Duck Bong Kim ),이관행 ( Kwan H. Lee ) 한국정보처리학회 2010 한국정보처리학회 학술대회논문집 Vol.17 No.1

RGB 영상의 색 재현성과 조건등색 등의 한계를 개선하기 위해 다중스펙트럼 영상을 활용하는 연구가 활발히 진행되고 있다. 다중스펙트럼 영상을 측정하기 위해서 흑백카메라와 3 개 이상의 컬러 필터들을 이용한다. 본 연구에서는 LCTF 를 사용하여 400nm 에서 720nm 까지 10nm 단위로 33 개의 필터를 사용한다. 이러한 다중스펙트럼 영상 측정 시스템의 특성화를 수행하기 위해 컬러체커뿐만 아니라 광원정보까지 이용하며, 사물의 스펙트럼 반사율도 추정한다. 따라서 분광방사계 보다 빠르게 영상 기반으로 스펙트럼 반사율을 측정할 수 있다. 다양한 광원 조건에서 특성화를 수행하며, 매번 레퍼런스 차트를 측정할 필요 없이 간단히 광원만 측정해도 되는 것을 보인다. 또한 전경영상에서 원하는 영역을 광원환경이 다른 배경 영상에 합성할 때, 자연스러운 사물의 색 변환이 가능하여 보다 실제에 가까운 합성 영상을 생성한다.

탄소펠트의 열처리 온도에 따른 레독스흐름전지와 전극 특성에 미치는 영향

유효성(Hyosung Yoo),유현진(Hyunjin You),유기현(Kihyun Yu),강준영(Junyoung Kang),박홍식(Hongsik Park),최웅휘(Woonghwi Choi),유동진(Dong Jin Yoo) 한국에너지학회 2015 에너지공학 Vol.24 No.3

생활습관중재가 부인암 환자의 피로와 영양상태 및 삶의 질에 미치는 효과

안현진(An, Hyunjin),노주희(Nho, Ju-Hee),유선영(Yoo, Sunyoung),김현민(Kim, Hyunmin),노민지(Nho, Minji),유호정(Yoo, Hojeong) 한국간호과학회 2015 Journal of Korean Academy of Nursing Vol.45 No.6