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Thinh Dinh Do,Yisoo Choi,Dae-Wui Jung,Chang-Bae Kim 한국동물분류학회 2020 Animal Systematics, Evolution and Diversity Vol.36 No.4
Mitochondrial genome is an important molecule for systematic and evolutionary studies in metazoans. The development of next-generation sequencing (NGS) technique has rapidly increased the number of mitogenome sequences. The process of generating mitochondrial genome based on NGS includes different steps, from DNA preparation, sequencing, assembly, and annotation. Despite the effort to improve sequencing, assembly, and annotation methods of mitogenome, the low quality and/or quantity sequence in the final map can still be generated through the work. Therefore, it is necessary to check and curate mitochondrial genome sequence after annotation for proofreading and feedback. In this study, we introduce the pipeline for sequencing and curation for mitogenome based on NGS. For this purpose, two mitogenome sequences of Dermatobranchus otome were sequenced by Illumina Miseq system with different amount of raw read data. Generated reads were targeted for assembly and annotation with commonly used programs. As abnormal repeat regions present in the mitogenomes after annotation, primers covering these regions were designed and conventional PCR followed by Sanger sequencing were performed to curate the mitogenome sequences. The obtained sequences were used to replace the abnormal region. Following the replacement, each mitochondrial genome was compared with the other as well as the sequences of close species available on the Genbank for confirmation. After curation, two mitogenomes of D. otome showed a typically circular molecule with 14,559 bp in size and contained 13 protein-coding genes, 22 tRNA genes, two rRNA genes. The phylogenetic tree revealed a close relationship between D. otome and Tritonia diomea. The finding of this study indicated the importance of caution and curation for the generation of mitogenome from NGS.
산업제어시스템의 소스코드 보안 취약점 검증 룰 선정을 위한 평가 기준 개발
김은비 ( Eunbi Kim ),최이수 ( Yisoo Choi ),한동준 ( Dongjoon Han ) 한국정보처리학회 2021 한국정보처리학회 학술대회논문집 Vol.28 No.2
산업제어시스템은 IT 기술의 발전에 따라 다양한 기기 환경과 네트워크를 적용해 진화하고 있다. 이러한 상황에서 사이버 보안의 위협은 가중되고 있으며, 이를 예방하는 방법의 하나로 산업제어시스템에 탑재되는 소프트웨어의 소스코드 개발 과정에서 보안 취약점을 예방하기 위해 소스코드 보안 룰을 적용하여 위반사항을 제거한다. 본 연구에서는 소스코드 보안 룰에서 적용 우선순위를 선정하기 위한 가이드를 개발한다.