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Choi, Min-Kyeung,Le, Minh Thong,Cho, Hyesun,Yum, Joori,Kang, Mingue,Song, Hyuk,Kim, Jin-Hoi,Chung, Hak Jae,Hong, Kwonho,Park, Chankyu Elsevier 2018 Gene Vol.640 No.-
<P><B>Abstract</B></P> <P>The sequence information of the genomic form of the human ABO blood group orthologous gene (erythrocyte antigen A, EAA) is not complete in pigs. Therefore, we cloned and characterized the nucleotide sequence of <I>EAA</I> intron 7, which is critical to understand genetic difference between <I>A</I> and <I>0</I> blood groups in pigs, covering complete genomic sequence information of <I>EAA</I> excluding a ~560bp unsequencible gap. We also analyzed genetic polymorphisms within <I>EAA</I> intron 7 and exon 8. We found difference in <I>A0</I> blood group frequencies among pig breeds. In addition, we designed a new genomic DNA-based <I>A0</I> blood group typing method and improved the accuracy and simplicity of the typing.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Improved the accuracy and simplicity of porcine <I>A0</I> blood group typing method. </LI> <LI> Covered complete genomic sequence information of porcine <I>EAA</I> and revealed the exact deletion and deletion boundary site in <I>0</I> allele. </LI> <LI> Identified difference in <I>A0</I> blood group frequencies among pig breeds </LI> </UL> </P>