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Antifungal Activity of Streptomyces sp. Against Puccinia recondite Causing Wheat Leaf Rust
YI, YONGSUB,KIM, SEUNGHYUN,KIM, MINWOO,CHOI, GYUNG JA,CHO, KWANG YUN,SONG, JAEKYEONG,LIM, YOONGHO 한국미생물 · 생명공학회 2004 Journal of microbiology and biotechnology Vol.14 No.2
To discover a potent strain against wheat leaf rust, soil samples collected from Ilgamho, Seoul, Korea were tested in vivo and a strain belonging to Streptomyces sp. was found to show good antifungal activity when fermented in a broth. The identification of the strain was carried out based on 16s rDNA analysis, and the active compound was separated from the fermented broth. Even though its structure was not determined completely, the authors report the results obtained so far indicate that the fermented broth of the strain showed activity against wheat leaf rust. Therefore, we propose that this may be a potential novel strain showing antifangal activity against Puccinia reconditu.
Antimicrobial Activity and Toxicity of Preservative Chemicals
Yongsub Yi 호서대학교 기초과학연구소 2016 기초과학연구 논문집 Vol.24 No.1
Parabens are alkyl esters of p-hydroxy benzoic acid which are widely used in foods, cosmetics, and pharmacological products as preservatives. Actually, human body is exposed to various parabens in the above products. This study was conducted to determine the toxicity of paraben by various concentration of butyl paraben, methyl paraben, propyl paraben, and 1,2 hexane diol in the three field of microorganisms, human keratinocyte, and zebra fish. The highest rate of growth inhibition was shown in microbial study of buthyl paraben. 1,2 hexane diol had the lowest cytotoxic effect and Methyl paraben had the highest in human keratinocyte. We have monitored the growth pattern of zebrafish at the time point (1,2,3, and 4 day). The toxicity induced the embryo mortality, morphological malformation, and disturbance in heart-beating. Methyl paraben was significantly increased the mortality rate in zebrafish embryo. In assessing the morphological malformations, most parabens did show the adverse effect, but 1,2 hexane diol did not shown.
Flavonoid Methylation of Poplar O-Methyltransferase Encoded in Escherichia coli
김봉규,Yongsub Yi 한국응용생명화학회 2009 Applied Biological Chemistry (Appl Biol Chem) Vol.52 No.6
O-Methyltransferases, which are involved in methylation of secondary metabolites, mediate the methylation of small molecules such as flavonoids, alkaloids, and plant hormones. Among them, flavonoids are an important class of secondary metabolites in plant and exhibit a wide spectrum of pharmacological properties. Modification of flavonoids extend and change pharmaceutical characterization of them as well as the structure. Populus deltoids caffeoyl CoA OMT gene (PopCCoAOMT) was amplified by RT-PCR, cloned and characterized. PopCCoAOMT had a homology of 94% and 90.8% with caffeoyl CoA OMTs of Nicotiana tabacum and Arabidopsis thaliana, and encoded 28.0 kDa in molecular weight. The reaction product of PopCCoAOMT enzyme methylated caffeoyl-CoA, quercetin, and luteolin to feruoyl-CoA, isorhamnetin, and chrisoeryol, respectively. PopCCoAOMT enzyme was shown to be active with compounds associated with substrates having an ortho hydroxyl group and a double bond at between 2 and 3 position of carbon.
Biotransformation of Eugenol to Bis-eugenol by Kalopanax pictus Cell Culture
Kim, Bong-Gyu,Kim, Jae Young,Yi, Yongsub,Lim, Yoongho The Korean Society for Applied Biological Chemistr 2012 Applied Biological Chemistry (Appl Biol Chem) Vol.55 No.5
So far, the production of bis-eugenol has been conducted by chemical synthesis. However, because chemical synthesis of bis-eugenol has disadvantages such as regioselectivity and low yield, an environmentally friendly production of bis-eugenol by biological synthesis has become attractive. In the present study, bis-eugenol was produced using Kalopanax pictus callus culture. High-performance liquid chromatography analysis of reaction product revealed a new peak, whose structure was determined to be bis-eugenol by nuclear magnetic resonance spectroscopy. After 18 h of biotransformation using K. pictus cell, 16.3 mg/L of biseugenol was produced without any byproducts. This is the first report on production of bis-eugenol by biotransformation using plant cell culture.
Isolation and Characterization of Streptomyces sp. KACC 91027 Against Plasmodiophora brassicae
KIM, SEUNGHYUN,SHIN, CHOONSHIK,MOON, SANGIK,YI, YONGSUB,CHOI, GYUNG JA,CHO, KWANG YUN,SONG, JAEKYEONG,LIM, YOONGHO 한국미생물 · 생명공학회 2004 Journal of microbiology and biotechnology Vol.14 No.1
Club root caused by Plasmodiophora brassicae is found in crucifers. Among the over hundreds of Streptomyces isolated from soil in Korea. One strain showed prominent activity against P. brassicae. The strain was identified based on 16S rDNA sequencing and the morphology by a method of scanning electron microscopy. An active compound in the fermented broth obtained from the strain was separated. Even though the complete assignments of the compound remain for future work, the results regarding the isolation and characterization of the strain with a certain activity against P. brassicae are shown in this paper.
천년초의 Taxifolin이 마우스대식세포(RAW 264.7 cell)에 미치는 항염증활성
김재영 ( Jaeyoung Kim ),이용화 ( Yonghwa Lee ),안현진 ( Hyeon Jin An ),이재덕 ( Jae Duk Lee ),이용섭 ( Yongsub Yi ) 한국응용생명화학회 2015 Journal of Applied Biological Chemistry (J. Appl. Vol.58 No.3
본 연구는 항산화 효과가 뛰어난 천년초를 이용하여 taxifolin을 순수분리하고 항염증 효과를 연구하였다. 천년초는 잎과 열매로 나누어 추출하였고 추출공정으로는 먼저 메탄올을 이용하여 조추출물을 추출하였다. 이렇게 얻어진 조추출물의 항산화 효과를 측정하였는데. 잎 추출물에서 38.33±1.07 μg/mL로 높은 항산화활성을 나타내었다. high performance liquid chromatography를 이용하여 taxifolin을 분리하고 확인하였다. 이렇게 얻어진 taxifolin을 이용하여 항염증 효과를 측정하였다. Taxifolin의 농도별 세포독성 측정에서는 500 μM 농도까지 처리 하였을 때 특별한 세포독성을 나타내지는 않았다. lipopolysaccharide에 의해 유도된 RAW 264.7 cell을 이용한 taxifolin의 염증성 cytokine측정실험에서는 IL-6의 발현양을 500 μM 처리시 2139.75±1.64pg/mL로 대조군에 비하여 47%까지 저해하였다. 하지만 TNF-α의 발현량은 대조군에 비하여 5%만 감소하였다. 염증성지표의 NO의 생성량은 500 μM 처리시 17.43±0.27 μM로 대조군에 비하여 57%까지 감소 시켰으며, 이와 관련된 COX-2 단백질의 발현양은 약 66% 이상 감소시켰다. 따라서 천년초에서 추출한 taxifolin은 항염증 효과가 있음을 확인할 수 있다. This study was performed to investigate the antiinflammatory activities of taxifolin from Opuntia humifusa. A potent anti-oxidant activity was shown from the leaf extract at IC50 value of 38.33±1.07 μg/mL and fruit extract at IC50 value of 40.23±2.21 μg/mL by 1,1-diphenyl-2-picrylhydrazyl assay. Fraction of taxifolin from leaf extract identified using high performance liquid chromatography and gas chromatography/mass spectrometry. The results of cell viability indicated that taxifolin did not show cytotoxicity on RAW 264.7 cells at 500 μM of concentration. The result showed that taxifolin inhibited lipopolysaccharide (LPS)-induced production of Nitrite oxide. In addition, taxifolin inhibited LPS-induced tumor necrosis factor-α and interleukin-6 production by cytokine assay and cyclooxygenase-2 expression by western blot analysis, meaning taxifolin has a significant anti-inflammatory effect. Our results suggested that taxifolin from Opuntia humifusa showed anti-inflammatory activities.