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Hui Li,Hong Xu,Sha Li,Hao Xu,Chaojiang Guo,Pingkai Ouyang,Hanjie Ying 한국생물공학회 2010 Biotechnology and Bioprocess Engineering Vol.15 No.5
Low-energy nitrogen ion beam implantation technique was used for the strain improvement of Alcaligenes sp. NX-3 for the production of exopolysaccharide welan gum. A high welan gum producing mutant, Alcaligenes sp. NX-3-1, was obtained through 20 keV N+ ion beam irradiation. Starting at a concentration of 50 g/L of glucose, mutant NX-3-1 produced 25.0 g/L of welan gum after 66 h of cultivation in a 7.5 L bioreactor, which was 34.4% higher than that produced by the wild-type strain. The results of metabolic flux analysis showed that the glucose-6-phosphate and acetyl coenzyme A nodes were the principle and flexible nodes, respectively. At the glucose-6-phosphate node, the fraction of carbon measured from glucose-6-phosphate to glucose-1-phosphate was enhanced after mutagenesis, which indicated that more flux was used to synthesize welan gum in the mutant. By analyzing the activities of related enzymes in the biosynthetic pathway of sugar nucleotides essential for welan gum production, we found that the specific activities of phosphoglucomutase, UDP-glucose pyrophosphorylase,UDP-glucose dehydrogenase, and dTDP-glucose pyrophosphorylase in the mutant strain were higher than those in the wild-type strain. These improvements in enzyme activities could be due to the affected of ion beam implantation.
Hui Xu,Chunzhi Wang,Hongwei Chen 보안공학연구지원센터 2016 International Journal of Grid and Distributed Comp Vol.9 No.11
When considering the coexistence of Software-Defined Networking (SDN) and non-SDN, unified management of SDN calls for study on both the information representation and the communication pattern. This paper first proposes the use of the extension theory in order to formally define management information of SDN in a unified manner. And this paper then introduces the concept of unified manager to the communication pattern for unified management of SDN, and considers application of the queuing theory from the perspective of the basic queue and the queue spaces. Finally, simulation results validate that, proposed approach based on the queuing theory is feasible for unified management of SDN from the viewpoint of the communication pattern.
Xu, Hui-Min,Zhu, Jian-Guo,Gu, Lian,Hu, Song-Qun,Wu, Hao Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.4
Vascular endothelial growth factor 2 (VEGFR2) was initially identified as a receptor of VEGF on endothelial cells with a role in regulating angiogenesis during organism development and tumorigenesis. Previously, in cancer tissue, VEGFR2 has been reported to be expressed in endothelial cells. In our research, we found that VEGFR2 was expressed not only in endothelial cells but also cancer cells in head and neck squamous cell carcinomas (HNSCCs). Knockdown of VEGFR2 in Hep2 cells could arrest the cell cycle in G0/G1, leading to a decrease in proliferation. We also present evidence that MAPK/ERK signal pathways and expression of CDK1 downstream of VEGFR2 might regulate proliferation and cell cycle arrest. Furthermore, we discovered that down-regulate VEGRF2 in Hep2 cells could significantly affect the invasion ability. Taken together, our data suggest that VEGFR2 might regulate proliferation and invasion in HNSCC cancer cells in vivo.
Research and Design of Automatic Control System of Hot Water Boiler Based On PLC
Xu Hui-jun 보안공학연구지원센터 2016 International Journal of Hybrid Information Techno Vol.9 No.7
In this paper, there are three scheme of PLC+ PC control program have been put forward for l0T/h hot water boiler. The multi variable, nonlinear, uncertain complex process automatic control is realized, and the thermal efficiency of the hot water boiler is improved. Each boiler in the system is controlled by the respective PLC, the public part is controlled by a set of PLC, an industrial control computer as an engineer station, an industrial control computer as an operator station, field bus Profibus-DP network was comprised by Industrial control computer, PLC. Fameview software was designed to achieve the redundant control of the upper monitor of the two boiler.