Genome-wide identification of long noncoding RNA genes and their potential association with mammary gland development in water buffalo

Jin Yuhan,Ouyang Yina,Fan Xinyang,Huang Jing,Guo Wenbo,Miao Yongwang 아세아·태평양축산학회 2022 Animal Bioscience Vol.35 No.11

Objective: Water buffalo, an important domestic animal in tropical and subtropical regions, play an important role in agricultural economy. It is an important source for milk, meat, horns, skin, and draft power, especially its rich milk that is the great source of cream, butter, yogurt, and many cheeses. In recent years, long noncoding RNAs (lncRNAs) have been reported to play pivotal roles in many biological processes. Previous studies for the mammary gland development of water buffalo mainly focus on protein coding genes. However, lncRNAs of water buffalo remain poorly understood, and the regulation relationship between mammary gland development/milk production traits and lncRNA expression is also unclear. Methods: Here, we sequenced 22 samples of the milk somatic cells from three lactation stages and integrated the current annotation and identified 7,962 lncRNA genes. Results: By comparing the lncRNA genes of the water buffalo in the early, peak, and late different lactation stages, we found that lncRNA gene lnc-bbug14207 displayed significantly different expression between early and late lactation stages. And lnc-bbug14207 may regulate neighboring milk fat globule-EGF factor 8 (MFG-E8) and hyaluronan and proteoglycan link protein 3 (HAPLN3) protein coding genes, which are vital for mammary gland development. Conclusion: This study provides the first genome-wide identification of water buffalo lncRNAs and unveils the potential lncRNAs that impact mammary gland development. Objective: Water buffalo, an important domestic animal in tropical and subtropical regions, play an important role in agricultural economy. It is an important source for milk, meat, horns, skin, and draft power, especially its rich milk that is the great source of cream, butter, yogurt, and many cheeses. In recent years, long noncoding RNAs (lncRNAs) have been reported to play pivotal roles in many biological processes. Previous studies for the mammary gland development of water buffalo mainly focus on protein coding genes. However, lncRNAs of water buffalo remain poorly understood, and the regulation relationship between mammary gland development/milk production traits and lncRNA expression is also unclear.Methods: Here, we sequenced 22 samples of the milk somatic cells from three lactation stages and integrated the current annotation and identified 7,962 lncRNA genes.Results: By comparing the lncRNA genes of the water buffalo in the early, peak, and late different lactation stages, we found that lncRNA gene <i>lnc-bbug14207</i> displayed significantly different expression between early and late lactation stages. And <i>lnc-bbug14207</i> may regulate neighboring milk fat globule-EGF factor 8 (<i>MFG-E8</i>) and hyaluronan and proteoglycan link protein 3 (<i>HAPLN3</i>) protein coding genes, which are vital for mammary gland development.Conclusion: This study provides the first genome-wide identification of water buffalo lncRNAs and unveils the potential lncRNAs that impact mammary gland development.

태양열 및 지열 이용 바닥복사난방시스템의 설계수법에 관한 해석적 연구

남유진(Nam, Yu-Jin),황석호(Hwang, Suck-Ho),까오신양(Gao, Xinyang) 대한건축학회 2013 대한건축학회 학술발표대회 논문집 Vol.33 No.2

Peripheral nerve defects repaired with autogenous vein grafts flled with platelet-rich plasma and active nerve microtissues and evaluated by novel multimodal ultrasound techniques

Yaqiong Zhu,Nan Peng,Jing Wang,Zhuang Jin,Lianhua Zhu,Yu Wang,Siming Chen,Yongqiang Hu,Tieyuan Zhang,Qing Song,Fang Xie,Lin Yan,Yingying Li,Jing Xiao,Xinyang Li,Bo Jiang,Jiang Peng,Yuexiang Wang,Yukun 한국생체재료학회 2022 생체재료학회지 Vol.26 No.3

Background: Developing biocompatible nerve conduits that accelerate peripheral nerve regeneration, lengthening and functional recovery remains a challenge. The combined application of nerve microtissues and platelet-rich plasma (PRP) provides abundant Schwann cells (SCs) and various natural growth factors and can compensate for the deficiency of SCs in the nerve bridge, as well as the limitations of applying a single type of growth factor. Multimodal ultrasound evaluation can provide additional information on the stiffness and microvascular flow perfusion of the tissue. This study was designed to investigate the effectiveness of a novel tissue-engineered nerve graft composed of an autogenous vein, nerve microtissues and PRP in reconstructing a 12-mm tibial nerve defect and to explore the value of multimodal ultrasound techniques in evaluating the prognosis of nerve repair. Methods: In vitro, nerve microtissue activity was first investigated, and the effects on SC proliferation, migration, factor secretion, and axonal regeneration of dorsal root ganglia (DRG) were evaluated by coculture with nerve microtissues and PRP. In vivo, seventy-five rabbits were equally and randomly divided into Hollow, PRP, Micro-T (Microtissues), Micro-T + PRP and Autograft groups. By analysing the neurological function, electrophysiological recovery, and the comparative results of multimodal ultrasound and histological evaluation, we investigated the effect of these new nerve grafts in repairing tibial nerve defects. Results: Our results showed that the combined application of nerve microtissues and PRP could significantly promote the proliferation, secretion and migration of SCs and the regeneration of axons in the early stage. The Micro-T + PRP group and Autograft groups exhibited the best nerve repair 12 weeks postoperatively. In addition, the changes in target tissue stiffness and microvascular perfusion on multimodal ultrasound (shear wave elastography; contrast-enhanced ultrasonography; Angio PlaneWave UltrasenSitive, AngioPLUS) were significantly correlated with the histological results, such as collagen area percentage and VEGF expression, respectively. Conclusion: Our novel tissue-engineered nerve graft shows excellent efficacy in repairing 12-mm defects of the tibial nerve in rabbits. Moreover, multimodal ultrasound may provide a clinical reference for prognosis by quantitatively evaluating the stiffness and microvescular flow of nerve grafts and targeted muscles

Verification of a tree canopy model and an example of its application in wind environment optimization

Yi Yang,Zhuangning Xie,Tim K.T. Tse,Xinyang Jin,Ming Gu 한국풍공학회 2012 Wind and Structures, An International Journal (WAS Vol.15 No.5

In this paper, the method of introducing additional source/sink terms in the turbulence and momentum transport equations was applied to appropriately model the effect of the tree canopy. At first, the new additional source term for the turbulence frequency ω equation in the SST k-ω model was proposed through theoretical analogy. Then the new source/sink term model for the SST k-ω model was numerically verified. At last, the proposed source term model was adopted in the wind environment optimal design of the twin high-rise buildings of CABR (China Academy of Building Research). Based on the numerical simulations, the technical measure to ameliorate the wind environment was proposed. Using the new inflow boundary conditions developed in the previous studies, it was concluded that the theoretically reasonable source term model of the SST k-ω model was applicable for modeling the tree canopy flow and accurate numerical results are obtained.

Verification of a tree canopy model and an example of its application in wind environment optimization

Yang, Yi,Xie, Zhuangning,Tse, Tim K.T.,Jin, Xinyang,Gu, Ming Techno-Press 2012 Wind and Structures, An International Journal (WAS Vol.15 No.5

In this paper, the method of introducing additional source/sink terms in the turbulence and momentum transport equations was applied to appropriately model the effect of the tree canopy. At first, the new additional source term for the turbulence frequency ${\omega}$ equation in the SST k-${\omega}$ model was proposed through theoretical analogy. Then the new source/sink term model for the SST k-${\omega}$ model was numerically verified. At last, the proposed source term model was adopted in the wind environment optimal design of the twin high-rise buildings of CABR (China Academy of Building Research). Based on the numerical simulations, the technical measure to ameliorate the wind environment was proposed. Using the new inflow boundary conditions developed in the previous studies, it was concluded that the theoretically reasonable source term model of the SST k-${\omega}$ model was applicable for modeling the tree canopy flow and accurate numerical results are obtained.