The CREG1-FBXO27-LAMP2 axis alleviates diabetic cardiomyopathy by promoting autophagy in cardiomyocytes

Liu Dan,Xing Ruinan,Zhang Quanyu,Tian Xiaoxiang,Qi Yanping,Song Haixu,Liu Yanxia,Yu Haibo,Zhang Xiaolin,Jing Quanmin,Yan Chenghui,Han Yaling 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-

Autophagy plays an important role in the development of diabetic cardiomyopathy. Cellular repressor of E1A-stimulated genes 1 (CREG1) is an important myocardial protective factor. The aim of this study was to investigate the effects and mechanisms of CREG1 in diabetic cardiomyopathy. Male C57BL/6 J mice, Creg1 transgenic mice and cardiac-specific knockout mice were used to establish a type 2 diabetes model. Small animal ultrasound, Masson’s staining and western blotting were used to evaluate cardiac function, myocardial fibrosis and autophagy. Neonatal mouse cardiomyocytes (NMCMs) were stimulated with palmitate, and the effects of CREG1 on NMCMs autophagy were examined. CREG1 deficiency exacerbated cardiac dysfunction, cardiac hypertrophy and fibrosis in mice with diabetic cardiomyopathy, which was accompanied by exacerbated autophagy dysfunction. CREG1 overexpression improved cardiac function and ameliorated cardiac hypertrophy and fibrosis in diabetic cardiomyopathy by improving autophagy. CREG1 protein expression was decreased in palmitate-induced NMCMs. CREG1 knockdown exacerbated cardiomyocyte hypertrophy and inhibited autophagy. CREG1 overexpression inhibited cardiomyocyte hypertrophy and improved autophagy. LAMP2 overexpression reversed the effect of CREG1 knockdown on palmitate-induced inhibition of cardiomyocyte autophagy. CREG1 inhibited LAMP2 protein degradation by inhibiting the protein expression of F-box protein 27 (FBXO27). Our findings indicate new roles of CREG1 in the development of diabetic cardiomyopathy.

활력 특징 분석을 통한 도시 역사적 장소의 디자인연구 : 중국의 지린시 우라 타운 사례로

류샤오린(Xiaolin Liu),김병옥(Byungok Kim) 한국디자인학회 2019 Design Works Vol.2 No.1

Considerations on Security and Trust Measurement for Virtualized Environment

Xiaolin Wang,Yan Sang,Yi Liu,Yingwei Luo 한국정보기술융합학회 2011 JoC Vol.2 No.2

Tuber borchii Shapes the Ectomycorrhizosphere Microbial Communities of Corylus avellana

( Xiaolin Li ),( Xiaoping Zhang ),( Mei Yang ),( Lijuan Yan ),( Zongjing Kang ),( Yujun Xiao ),( Ping Tang ),( Lei Ye ),( Bo Zhang ),( Jie Zou ),( Chengyi Liu ) 한국균학회 2019 Mycobiology Vol.47 No.2

In this study, eight-month-old ectomycorrhizae of Tuber borchii with Corylus avellana were synthesized to explore the influence of T. borchii colonization on the soil properties and the microbial communities associated with C. avellana during the early symbiotic stage. The results showed that the bacterial richness and diversity in the ectomycorrhizae were significantly higher than those in the control roots, whereas the fungal diversity was not changed in response to T. borchii colonization. Tuber was the dominant taxon (82.97%) in ectomycorrhizae. Some pathogenic fungi, including Ilyonectria and Podospora, and other competitive mycorrhizal fungi, such as Hymenochaete, had significantly lower abundance in the T. borchii inoculation treatment. It was found that the ectomycorrhizae of C. avellana contained some more abundant bacterial genera (e.g., Rhizobium, Pedomicrobium, Ilumatobacter, Streptomyces, and Geobacillus) and fungal genera (e.g., Trechispora and Humicola) than the control roots. The properties of rhizosphere soils were also changed by T. borchii colonization, like available nitrogen, available phosphorus and exchangeable magnesium, which indicated a feedback effect of mycorrhizal synthesis on soil properties. Overall, this work highlighted the interactions between the symbionts and the microbes present in the host, which shed light on our understanding of the ecological functions of T. borchii and facilitate its commercial cultivation.

The Effect of Hot Extrusion on the Structure and Thermoelectric Properties of Material

Liu Xuequan,Ren Wei,Han Wei,Wang Xiaolin 한국분말야금학회 2006 한국분말야금학회 학술대회논문집 Vol.2006 No.1

Transplantation of Gelatin Microspheres Loaded with Wharton's Jelly Derived Mesenchymal Stem Cells Facilitates Cartilage Repair in Mice

Chen Xiaolin,Huang Sunxing,Niu Yongxia,Luo Mingxun,Liu Haiying,Jiao Yiren,Huang Junjiu 한국조직공학과 재생의학회 2024 조직공학과 재생의학 Vol.21 No.1

Background: Knee osteoarthritis (KOA) is a prevalent chronic joint disease caused by various factors. Mesenchymal stem cells (MSCs) therapy is an increasingly promising therapeutic option for osteoarthritis. However, the chronic inflammation of knee joint can severely impede the therapeutic effects of transplanted cells. Gelatin microspheres (GMs) are degradable biomaterial that have various porosities for cell adhesion and cell–cell interaction. Excellent elasticity and deformability of GMs make it an excellent injectable vehicle for cell delivery. Methods: We created Wharton’s jelly derived mesenchymal stem cells (WJMSCs)-GMs complexes and assessed the effects of GMs on cell activity, proliferation and chondrogenesis. Then, WJMSCs loaded in GMs were transplanted in the joint of osteoarthritis mice. After four weeks, joint tissue was collected for histological analysis. Overexpressing-luciferase WJMSCs were performed to explore cell retention in mice. Results: In vitro experiments demonstrated that WJMSCs loaded with GMs maintained cell viability and proliferative potential. Moreover, GMs enhanced the chondrogenesis differentiation of WJMSCs while alleviated cell hypertrophy. In KOA mice model, transplantation of WJMSCs-GMs complexes promoted cartilage regeneration and cartilage matrix formation, contributing to the treatment of KOA. Compared with other groups, in WJMSCs+GMs group, there were fewer cartilage defects and with a more integrated tibia structure. Tracking results of stable-overexpressing luciferase WJMSCs demonstrated that GMs significantly extended the retention time of WJMSCs in knee joint cavity. Conclusion: Our results indicated that GMs facilitate WJMSCs mediated knee osteoarthritis healing in mice by promoting cartilage regeneration and prolonging cell retention. It might potentially provide an optimal strategy for the biomaterial-stem cell based therapy for knee osteoarthritis. Background: Knee osteoarthritis (KOA) is a prevalent chronic joint disease caused by various factors. Mesenchymal stem cells (MSCs) therapy is an increasingly promising therapeutic option for osteoarthritis. However, the chronic inflammation of knee joint can severely impede the therapeutic effects of transplanted cells. Gelatin microspheres (GMs) are degradable biomaterial that have various porosities for cell adhesion and cell–cell interaction. Excellent elasticity and deformability of GMs make it an excellent injectable vehicle for cell delivery. Methods: We created Wharton’s jelly derived mesenchymal stem cells (WJMSCs)-GMs complexes and assessed the effects of GMs on cell activity, proliferation and chondrogenesis. Then, WJMSCs loaded in GMs were transplanted in the joint of osteoarthritis mice. After four weeks, joint tissue was collected for histological analysis. Overexpressing-luciferase WJMSCs were performed to explore cell retention in mice. Results: In vitro experiments demonstrated that WJMSCs loaded with GMs maintained cell viability and proliferative potential. Moreover, GMs enhanced the chondrogenesis differentiation of WJMSCs while alleviated cell hypertrophy. In KOA mice model, transplantation of WJMSCs-GMs complexes promoted cartilage regeneration and cartilage matrix formation, contributing to the treatment of KOA. Compared with other groups, in WJMSCs+GMs group, there were fewer cartilage defects and with a more integrated tibia structure. Tracking results of stable-overexpressing luciferase WJMSCs demonstrated that GMs significantly extended the retention time of WJMSCs in knee joint cavity. Conclusion: Our results indicated that GMs facilitate WJMSCs mediated knee osteoarthritis healing in mice by promoting cartilage regeneration and prolonging cell retention. It might potentially provide an optimal strategy for the biomaterial-stem cell based therapy for knee osteoarthritis.

A Complex Reinforced Polymer Interposer with Ordered Ni Grid and SiC Nano-whiskers Polyimide Composite Based on Micromachining Technology

Yanmei Liu,Yunna Sun,Yan Wang,Guifu Ding,Bin Sun,Xiaolin Zhao 대한금속·재료학회 2017 ELECTRONIC MATERIALS LETTERS Vol.13 No.1

A complex reinforced polymer interposer comprised with conductive Nicylinders, ordered Ni grid and SiC nano-whiskers/Polyimide (PI)composite was proposed. The conductive Ni cylinders distributing in themiddle of each Ni grid unite designed as the supporting structure wereused as electric connecting component for the interposer and wereinsulated by the SiC nano-whiskers/PI composite. The comprehensiveproperties of the complex reinforced polymer interposer were improvedby a complex reinforced mechanism: the improved thermal conductivityand mechanical strength by the Ni supporting structure and the reducedmetal/polymer interfacial mismatch due to the SiC nano-whiskers/PIcomposite with the optimized mixture ratio. The above complexreinforced polymer interposer and a traditional reinforced polymerinterposer only with Ni grid were fabricated using micro-machiningtechnology for comparative analysis. The comprehensive properties ofthese two polymer interposers were analyzed respectively. Comparedwith the traditional design, the comprehensive properties of the proposedcomplex reinforced polymer interposer were improved further, such as,21.3% increase for the Young modulus, 10.1% decrease for thecoefficient of thermal expansion (CTE) and 54.9% increase for thethermal conductivity. Such complex reinforced mechanism based on themetal ordered grid and random nano-whiskers has potential to expandthe applications of the polymer interposer.

Improved Performances of AlN/Polyimide Hybrid Film and its Application in Redistribution Layer

Zhe Liu,Guifu Ding,Jiangbo Luo,Wen Lu,Xiaolin Zhao,Ping Cheng,Yanlei Wang 대한금속·재료학회 2016 ELECTRONIC MATERIALS LETTERS Vol.12 No.5

The AlN/polyimide (PI) hybrid film was studied as thedielectric layer in the redistribution layer (RDL) in this work. The incorporation of the AlN into the PI matrix was achievedby mechanical ball-milling process. The spin-coating processwas used to fabricate the AlN/PI hybrid film, which iscompatible with micro-electro-mechanical system (MEMS)technology for fabricating RDL. The AlN/PI hybrid film wascharacterized by Fourier transform infrared (FTIR) spectrumand thermogravimetric analysis (TGA). The effect of the AlNcontent on the thermal stability, thermal expansion coefficient,hardness and water adsorption of the AlN/PI hybrid film wasstudied. The results indicated that the addition of AlN nanoparticlesimproved the thermal stability and hardness, butdecreased the thermal expansion coefficient and waterabsorption of the pure PI film. As an example of its typicalapplication, the AlN/PI hybrid film with 8 wt.% AlN waspatterned using micromachining technology and used as thedielectric layer in RDL successfully.

De novo transcriptomic analysis of gonad of Strongylocentrotus nudus and gene discovery for biosynthesis of polyunsaturated fatty acids

Zhenlin Wei,Xiaolin Liu,Zunchun Zhou,Junxiao Xu 한국유전학회 2019 Genes & Genomics Vol.41 No.5

Background Strongylocentrotus nudus is an important cultured sea urchin species in north China, because its gonad is rich in unsaturated fatty acids, particularly long polyunsaturated fatty acids (LC-PUFAs). These PUFAs play pleiotropic and crucial roles in a wide range of biological process. Objective However, the genes contributing to biosynthesis PUFAs have not been elucidated yet, and the molecular mechanism relative to the difference in PUFA composition between male and female gonad as been revealed but the corresponding has not been understood. Methods In this paper, solexa sequencing based transcriptomic approach was used to identify and characterize the key genes relative to PUFA synthesis and further conducted different expressed genes between male and female gonad. Results A total of 130,124 transcripts and 189330 unigenes were de novo assembled from 64.32 Gb data. Next, these unigenes were subjected to functional annotation by mapping to six public databases, and this process revealed a lot of genes involving in lipid metabolism. In addition, three types of fatty acids front-end desaturase and three species of very long fatty acids elongase were identified and the pathway for PUFA biosynthesis was hypothesized. Last, comparative analysis revealed the higher expression level of Δ5 desaturase, Δ6 desaturase, ELOVL-4, -6 and -7 in male gonad compared with female. Conclusion This results could plausible explain the differ in composition of PUFAs between male and female gonad of sea urchin.

Structural basis for synaptic adhesion mediated by neuroligin-neurexin interactions

Chen, Xiaoyan,Liu, Heli,Shim, Ann H R,Focia, Pamela J,He, Xiaolin Nature Publishing Group 2008 Nature Structural and Molecular Biology Vol. No.

The heterophilic synaptic adhesion molecules neuroligins and neurexins are essential for establishing and maintaining neuronal circuits by modulating the formation and maturation of synapses. The neuroligin-neurexin adhesion is Ca<SUP>2+</SUP>-dependent and regulated by alternative splicing. We report a structure of the complex at a resolution of 2.4 Å between the mouse neuroligin-1 (NL1) cholinesterase-like domain and the mouse neurexin-1β (NX1β) LNS (laminin, neurexin and sex hormone–binding globulin–like) domain. The structure revealed a delicate neuroligin-neurexin assembly mediated by a hydrophilic, Ca<SUP>2+</SUP>-mediated and solvent-supplemented interface, rendering it capable of being modulated by alternative splicing and other regulatory factors. Thermodynamic data supported a mechanism wherein splicing site B of NL1 acts by modulating a salt bridge at the edge of the NL1-NX1β interface. Mapping neuroligin mutations implicated in autism indicated that most such mutations are structurally destabilizing, supporting deficient neuroligin biosynthesis and processing as a common cause for this brain disorder.