탈지 환원유에서 Bifidobacterium longum의 호기성 고농도 배양에 관한 연구

구동환,하상우,조수현,박기문,김동운,강국희 성균관대학교 생명과학자원연구소 1997 生命資源科學硏究 Vol.4 No.1

The objectives of this study were to investigate the optimal condition for growth and highly concenterated culture of Bifidobacterium longum (Bif longum). The effects of additives were compared with titratable acidity and viable cell counts, and 10% reconstituted skim milk (no additive) was used as a control. Correlation coefficient for the growth of Bif. longun between CO_2 anaerobic jar method and overalaid medium method was R=0.99. When 1% Bif. longurn was inoculated on 12%, 14%, and 16% reconstituted skim-milk, viable cell counts were 8.60, 8.89, and 8.93 Log CFU/ml, respectively. When Lactose, Glucose, Glucosamine, Fructose, Peptone, N-acethyl-D-glucosamine, D-galactose, and liver extract were added to 16% reconstituted skim-milk, the viable cell counts were 8.61, 9.05, 9.15, 9.24, 8.82, 9.75, 9.17, and 9.64 Log CFU/ml, respectively. Therefore, D-galactose, liver extract, and N-acethyl-D-glucosamine were selected as final additives. When Bif longum was inoculated on Fermentor-I (16% reconstituted skim milk containing 0.1% yeast extract, 0.5% D-galactose and 0.5% liver extract), Fermentor-II (16% reconstituted skim milk containing 0.190% yeast extract, 0.590% N-acethyl-D-glucosamine and 0.5% liver extract), Batch culture-I (16% reconstituted skim milk containing 0.1% yeast extract, 0.5% D-galactose and 0.5% liver extract), and Batch culture-II (16% reconstituted skim milk containing 0.1% yeast extract, 0.5% N-acethyl-D-glucosamine and 0.5% liver extract), viable cell counts were 10.46, 9.37, 9.03, and 8.83 Log CFU/ml, respectively. Although liver extract was the important additive for the highly concentrated culture of Bif. longum in this study, it caused serious off-flavor when liver extract-1 (contained cell mass) was added to Fermentor culture. However, flavor was improved when liver extract-2 which was removed cell mass, was used instead of liver extract-1. Addition of 2% liver extract-II and 1% D-galactose had the similar effect with addition of 0.5% Liver extract-I and 0.5% D-galactose on the growth of Bif. longum.

Effect of Mechanical Ventilation Mode Type on Intra- and Postoperative Blood Loss in Patients Undergoing Posterior Lumbar Interbody Fusion Surgery: A Randomized Controlled Trial

Kang, Woon-Seok,Oh, Chung-Sik,Kwon, Won-Kyoung,Rhee, Ka Young,Lee, Yun Gu,Kim, Tae-Hoon,Lee, Suk Ha,Kim, Seong-Hyop by 2016, the American Society of Anesthesiologists 2016 Anesthesiology Vol.125 No.1

<P>Background: The aim of study was to evaluate the effect of mechanical ventilation mode type, pressure-controlled ventilation (PCV), or volume-controlled ventilation (VCV) on intra-and postoperative surgical bleeding in patients undergoing posterior lumbar interbody fusion (PLIF) surgery. Methods: This was a prospective, randomized, single-blinded, and parallel study that included 56 patients undergoing PLIF and who were mechanically ventilated using PCV or VCV. A permuted block randomization was used with a computer-generated list. The hemodynamic and respiratory parameters were measured after anesthesia induction in supine position, 5 min after patients were changed from supine to prone position, at the time of skin closure, and 5 min after the patients were changed from prone to supine position. The amount of intraoperative surgical bleeding, fluid administration, urine output, and transfusion requirement were measured at the end of surgery. The amount of postoperative bleeding and transfusion requirement were recorded every 24 h for 72 h. Results: The primary outcome was the amount of intraoperative surgical bleeding, and 56 patients were analyzed. The amount of intraoperative surgical bleeding was significantly less in the PCV group than that in the VCV group (median, 253.0 [inter-quartile range, 179.0 to 316.5] ml in PCV group vs. 382.5 [328.0 to 489.5] ml in VCV group; P < 0.001). Comparing other parameters between groups, only peak inspiratory pressure at each measurement point in PCV group was significantly lower than that in VCV group. No harmful events were recorded. Conclusion: Intraoperative PCV decreased intraoperative surgical bleeding in patients undergoing PLIF, which may be related to lower intraoperative peak inspiratory pressure.</P>

항결핵제 치료시 발생하는 부작용

이해운,김성렬,강동구,이준상,유광하,진춘조 건국대학교 의과학연구소 2003 건국의과학학술지 Vol.13 No.-

One hundred thirty seven patients with pulmonary and extrapulmonary tuberculosis who visited Konkuk University Hospital between July 1996 and July 2002, were treated with isoniazid, rifampin, ethambutol, and pyrazinamide. Among these patients, ten patients had underlying diabetes mellitus, and three HB viral hepatitis. Their medical chart were reviewed retrospectively to evaluate the adverse effects of anti -tuberculous medication. The major side effects of patients are gastrointestinal intolerance(26.4%) and hepatotoxicity(25.3%). Minor side effects are itching sensation, fever, skin eruption, myalgia, arthlagia, and other hypersensitivity reaction. We evaluated the onset, duration, and management of side effect during TB medication according to underlying diseases

물리치료사의 직무유형이 직무만족도와 조직몰입도에 미치는 영향 : 부산지역을 중심으로

김성구 ( Seong Gu Kim ),노정운 ( Jung Woon No ),박민규 ( Ming Yu Park ),하용목 ( Yong Mok Ha ),김지혁 ( Chi Hyok Kim ),배원식 ( Won Sik Bae ) 대한통합의학회 2014 대한통합의학회지 Vol.2 No.4

Purpose : The purpose of this survey was to investigate the effect on job satisfaction and organizational commitment according to the job types of Pusan area physical therapists figuring out structural causality between job satisfaction and organizational commitment. Method : This survey was conducted through structured questionnaire for the physical therapists working in Pusan area, and data from 217 therapists were utilized in the final analysis. Data collection was deployment questionnaire using Google Docs (online survey), The SPSS 21.0 program was used according to for research purposes independent t-test was used for individual and job characteristics, was used according to job types and job satisfaction and organizational commitment are logistic regression analysis, correlation analysis and chi-square test. Result : The results of this study were as follows. 1. Overall job satisfaction according to the job types was appeared musculoskeletal physical therapist more 4 times. 2. Job types according to the organizational commitment did not make direct effect. Conclusion : Number of musculoskeletal physical therapist than neurological physical therapist was fewer and there were many per day treatment of patients, but overall job satisfaction was appeared that is higher. This is thought that job types affects more than working environment.

Experience in Combustion of Various Dewatered Sludges at a Commercial-Scale Fluidized Bed Incinerator

Jae Hoi Gu,Byung Sang Yoo,Woon Ha Yeo,Yong Chil Seo,Jea Keun Lee 한국에너지학회 2000 한국에너지공학회 학술발표회 Vol.2000 No.-

The Characteristics of Co-Incineration of Dewatered Sludge , Waste Oil and Waste Solvent in Commercial - Scale Fluidized Bed Incinerator

(Jae Hoi Gu),(Woon Ha Yeo),(Yong Chil Seo),(Sang Hoon Lee),(Jea Keun Lee) 한국화학공학회 2002 Korean Journal of Chemical Engineering Vol.19 No.2

Temporal Expression of Interleukin-1${\beta}$ in the Dorsal Root Ganglion in a Rat Model of Lumbar Disc Herniation

Kim, Su-Jeong,Gu, Hee-Jin,Cho, Yun-Woo,Park, Hea-Woon,Lee, Joon-Ha,Hwang, Se-Jin,Ahn, Sang-Ho The Korean Society of Physical Therapy 2010 대한물리치료학회지 Vol.22 No.3

Purpose: To investigate temporal changes in IL-1${\beta}$ mRNA expression in spinal dorsal horn (DH) and dorsal root ganglion (DRG) in a rat lumbar disc herniation (LDH) model. Methods: Autologous nucleus pulposus, harvested from the tail disc between the second and third coccygeal vertebrae (Co2-3), was implanted next to the left L5 nerve root just proximal to the DRG after partial laminectomy. IL-1${\beta}$ mRNA expression was investigated in DRG and DH in our LDH model. Real-time PCR assays were done using a 7500 Real Time PCR system (Applied Biosystems, USA). Results: Expression of IL-1${\beta}$ in DRG and DH was observed for 30 days postoperatively. Expression of IL-1${\beta}$ mRNA in the ipsilateral DRG of the LDH group gradually increased from 5 to 30 days after surgery. The amount of IL-1${\beta}$ in the contralateral DRG peaked 10 days after surgery and then gradually decreased. However, there was no difference in IL-1${\beta}$ mRNA expression in spinal DH between the LDH group and the sham-operated group. Conclusion: Long-term expression of IL-1${\beta}$ in the LDH model may worsen the chronic pain state. Future studies on inhibition of IL-1${\beta}$ expression in the LDH model will be needed to develop selective treatment strategies for patients with LDH.

활동관찰이 축구드리블 과제 학습에 미치는 영향

하태호 ( Tae Ho Ha ),김성운 ( Sung Woon Kim ),김진구 ( Jin Gu Kim ),박상범 ( Sang Bum Park ) 경북대학교 중등교육연구소 2012 중등교육연구 Vol.60 No.1

이 연구의 목적은 활동관찰이 개방기술인 축구드리블 과제 학습에 미치는 영향을 분석함으로써 운동기능학습의 촉진을 위한 활동관찰 프로그램의 적용 가능성을 알아보는데 있다. 실험에 참여한 피험자는 만14세의 남자 중학교 2학년 40명으로 축구드리블 수행에 아무런 장애가 없는 신체적, 정신적으로 건강한 오른발잡이들로만 참여하였다. 축구드리블 동작수행 시간에 따른 집단(4)×측정시점(4)에 대한 반복측정 이원분산분석을 실시하였고, Tukey`s HSD를 사용하여 사후검증을 실시하였다. 종속변수는 축구드리블 동작수행 시간이다. 연구결과 활동관찰은 환경이 변화하고 예측하기 어려운 특성을 지니고 있는 개방기술인 축구드리블 기술학습을 촉진시킬 수 있음을 보여주었다. 또한 활동관찰은 신체연습과 병행 되었을 때 가장 큰 효과를 지닐 수 있으며, 활동관찰과 신체연습의 병행은 어느 한 가지 연습방법을 통해 얻을 수 있는 것 이상의 학습경험을 제공할 수 있음을 보여주었다. 그러므로 이 연구 결과를 종합해 보면 운동기능 학습에서 활동관찰은 운동기능의 학습을 촉진 시킬 수 있는 인지적 중재방법으로써 그 가능성이 높다고 사료된다. The purpose of this study was to examine the effectiveness of action observation on the performance and learning of a soccer dribbles task. Forty middle school students whose ages ranged from 14 to 15 years (M=14. 00) participated in this experiment. The participants were randomly assigned to one of four experimental conditions: (1) action observation group(n=10), (2) physical practice group(n=10), (3) simultaneous group which practiced AO and PP alternatively(n=10), (4) control group (n=10). Data was analyzed using two-way ANOVAs with repeated measures of group(4) and time(4). Dependent variables were soccer dribbles time. The results of this study indicated that the AO, PP, and simultaneous group were also superior to the control group, and in particular the simultaneous group was faster than the AO, PP group in response time for soccer dribbles. These results suggests that action observation can facilitate the performance of a soccer dribbles and its effect can be maximized when performed simultaneously with the physical practice.

Identification of QTLs Associated with Cold Tolerance under High Elevation Rice Growing Area in Rice(O. sativa L.)

You-Chun Song,Jun-Hyeon Cho,Woon-Gu Ha,Ji-Yoon Lee,No-Bong Park,Jin-Il Choung,Shambhu P. Khatiwada,T. Akhtar,B. Chaudhary,Byeon-Geun Oh 한국작물학회 2008 한국작물학회 학술발표대회 논문집 Vol.2008 No.10

Cold sensitivity has been shown to vary and the cold tolerance from vegetative to reproductive stage appears to be independent during the life cycle. In the current study, cold tolerance under high elevation rice growing area were evaluated using F4 generation of 181 lines derived from Gayabyeo*2/Chhamrong. Five main-effect QTLs related to days to heading, panicle exertion and spikelet fertility were identified. The QTL qDH-3 on chromosome 3 showed the peak LOD score of 6.3, explaining up to 16.5% of phenotypic variation with additive effect of -2.6. Moreover, the QTL qPE-3 and qSF-3 on chromosome 3 were coincided with the QTL qDH-3 showing an opposite allele effects. Thus, the region harboring marker RM523-RM14281 could be helpful for the selection of cold tolerance genotypes in marker assisted selection(MAS) of rice breeding program.

Identification of QTLs Associated with Cold Tolerance in Rice(O. sativa L.)

You-Chun Song,Jun-Hyeon Cho,Woon-Gu Ha,Ji-Yoon Lee,No-Bong Park,Jin-Il Choung,Do-Yeon Kwak,Min-Hee Nam,Shambhu P. Khatiwada,T. Akhtar,B. Chaudhary,Byeon-Geun Oh 한국작물학회 2008 한국작물학회 학술발표대회 논문집 Vol.2008 No.10

Cold tolerance at every growing stage of rice(Oryza sativa L.) is one of the main determinations for the stable growth in temperature and high elevate area. In the current study, a 181 lines of BC population derived from a cross of Gayabyeo, a Tongil type sensitive to cold and Chhamrong a tolerant to cold were evaluated for cold tolerance with cold water irrigation(17℃) at seeding stage as well as low temperature germinate at 13℃, respectively. The resulting linkage map consists of 157 marker loci, covering all of 12 rice chromosomes and spanning 910cM(Haldane function) with an average interval of 76cM between markers. Three main-effect QTLs were identified. The comparison of the OTLs identified in this cold treatments resulted in an intriquing finding that each treatments were controlled by a major QTL. The QTL qCWI-4 on chromosome 4 was found to increase its additive effect to -0.84 as the cold water irrigate stress was given. In the meanwhile, the QTL qLTG-8 was detected with a LOD score of5.54, explaining up to 13% of the phenotypic variation controlled by Gayabyeo allele. In addition, the QTL qLTGV-3, controlled by Gayabyeo allele with a LOD score of 5.19 explaining about 12.5% of the variation was also identified. These results would favor our better understanding of the genetic control of cold tolerance in rice and be important for the development of rice cultivars with a broaden climatic adaptation.