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( Won Jung Koh ),( Bok Soon Chang ),( Byeong Ho Jeong ),( Kyeong Man Jeon ),( Su Young Kim ),( Nam Yong Lee ),( Chang Seok Ki ),( O Jung Kwon ) 대한결핵 및 호흡기학회 2013 Tuberculosis and Respiratory Diseases Vol.75 No.5
Background: The number of patients with pulmonary disease caused by nontuberculous mycobacteria (NTM) has been increasing worldwide. The aim of this study was to evaluate long-term trends in the NTM recovery rate from respiratory specimens over a 10-year period in a tertiary referral hospital in South Korea. Methods: We retrospectively reviewed the records of mycobacterial cultures of respiratory specimens at Samsung Medical Center from January 2001 to December 2011. Results: During the study period, 32,841 respiratory specimens from 10,563 patients were found to be culture-positive for mycobacteria. These included 12,619 (38%) Mycobacterium tuberculosis and 20,222 (62%) NTM isolates. The proportion of NTM among all positive mycobacterial cultures increased from 43% (548/1,283) in 2001 to 70% (3,341/4,800) in 2011 (p<0.001, test for trend). The recovery rate of NTM isolates from acid-fast bacilli smear-positive specimens increased from 9% (38/417) in 2001 to 64% (1,284/1,997) in 2011 (p<0.001, test for trend). The proportion of positive liquid cultures was higher for NTM than for M. tuberculosis (p<0.001). The most frequently isolated NTM were Mycobacterium avium-intracellulare complex (53%) and Mycobacterium abscessus-massiliense complex (25%). Conclusion: The recovery rate of NTM from respiratory specimens in South Korea has increased steadily.
Jeon, Janet Ye-Won,Yoo, Seung-Hyun,Kim, Hye-Kyeong Korean Society for Health Education and Promotion 2012 보건교육건강증진학회지 Vol.29 No.2
Objectives: The objective of the study was to examine the clustering patterns and correlates of multiple health behaviors (MHBs) in middle-aged Koreans with metabolic syndrome (MetS). Methods: Data on sociodemographics, clinical characteristics, health behaviors (vegetable intake, physical activity, cigarette smoking, and alcohol consumption), and psychological characteristics were collected by a self-reported survey and medical examination from 331 individuals with MetS. Clustering of MHBs was examined by measuring 1) the ratios of observed and expected prevalence of MHBs, and 2) the prevalence odds ratios. A binomial logistic regression were conducted. Results: Men were more likely than women to engage in multiple unhealthy behaviors. Clustering of smoking and heavy drinking was exhibited in the participants. Women with high vegetable intake were more likely to be physically inactive, and those with inadequate vegetable intake were more likely to be physically active. Those with lower self-regulation were more likely to engage in unhealthy behaviors. Conclusions: The findings support the multiple health behavior approach as opposed to the individual health behavior approach. Emphasis of self-regulation is necessary in developing multiple behavior intervention for individuals with MetS.
Kyeong Mi Kim,Nam Seon Kang,Hyeong Seok Jang,Joon Sang Park,Byung Hee Jeon,Ji Won Hong 한국해양바이오학회 2017 한국해양바이오학회지 Vol.9 No.2
A unicellular green alga was axenically isolated from the Port of Jeongja, Ulsan, Korea. Morphological, molecular, and biochemical analyses revealed that the isolate belonged to Heterochlorella luteoviridis. This is the first report of this species in Korea. The microalgal strain was named as H. luteoviridis MM0014 and its growth, lipid composition, and biomass properties were investigated. The strain thrived over a wide range of temperatures (5–30°C) and withstood up to 0.5 M NaCl. The results of gas chromatography/mass spectrometry analysis showed that the isolate was rich in nutritionally important polyunsaturated fatty acids. Its major fatty acids were linoleic acid (35.6%) and α-linolenic acid (16.2%). Thus, this indigenous marine microalga is a potential alternative source of ω3 and ω6 polyunsaturated fatty acids, which are currently obtained from fish and plant oils. Ultimate analysis indicated that the gross calorific value was 19.7 MJ kg-1. In addition, the biomass may serve as an excellent animal feed because of its high protein content (51.5%). Therefore, H. luteoviridis MM0014 shows promise for applications in the production of microalgae-based biochemicals and biomass feedstock.
Jeon, Bu-Nam,Kim, Min-Kyeong,Yoon, Jae-Hyeon,Kim, Min-Young,An, Haemin,Noh, Hee-Jin,Choi, Won-Il,Koh, Dong-In,Hur, Man-Wook Oxford University Press 2014 Nucleic acids research Vol.42 No.18
<P>ZNF509 is unique among POK family proteins in that four isoforms are generated by alternative splicing. Short ZNF509 (ZNF509S1, -S2 and -S3) isoforms contain one or two out of the seven zinc-fingers contained in long ZNF509 (ZNF509L). Here, we investigated the functions of ZNF509 isoforms in response to DNA damage, showing isoforms to be induced by p53. Intriguingly, to inhibit proliferation of HCT116 and HEK293 cells, we found that ZNF509L activates <I>p21/CDKN1A</I> transcription, while ZNF509S1 induces <I>RB</I>. ZNF509L binds to the <I>p21/CDKN1A</I> promoter either alone or by interacting with MIZ-1 to recruit the co-activator p300 to activate <I>p21/CDKN1A</I> transcription. In contrast, ZNF509S1 binds to the distal <I>RB</I> promoter to interact and interfere with the MIZF repressor, resulting in derepression and transcription of <I>RB</I>. Immunohistochemical analysis revealed that ZNF509 is highly expressed in normal epithelial cells, but was completely repressed in tumor tissues of the colon, lung and skin, indicating a possible role as a tumor suppressor.</P>
Bronchoscopic Findings of Pulmonary Paragonimiasis
( Kyeong Man Jeon ),( Jae Uk Song ),( Sang Won Um ),( Won Jung Koh ),( Gee Young Suh ),( Man Pyo Chung ),( O Jung Kwon ),( Joung Ho Han ),( Ho Joong Kim ) 대한결핵 및 호흡기학회 2009 Tuberculosis and Respiratory Diseases Vol.67 No.6
Background: Pulmonary paragonimiasis is a subacute to chronic inflammatory disease of the lung caused by lung flukes that result in prolonged inflammation and mechanical injury to the bronchi. However, there are few reports on the bronchoscopic findings of pulmonary paragonimiasis. This report describes the bronchoscopic findings of pulmonary paragonimiasis. Methods: The bronchosocpic findings of 30 patients (20 males, median age 50 years) with pulmonary paragonimiasis between May 1995 and December 2007 were reviewed retrospectively. Results: The diagnoses were based on a positive serologic test results for Paragonimus-specific antibodies in 13 patients (43%), or the detection of Paragonimus eggs in the sputum, bronchial washing fluid, or lung biopsy specimens in 17 patients (57%). The bronchoscopic examinations revealed endobronchial lesions in 17 patients (57%), which were located within the segmental bronchi in 10 patients (59%), lobar bronchi in 6 patients (35%) and main bronchi in 1 patient (6%). The bronchoscopic characteristics of endobronchial lesions were edematous swelling of the mucosa (16/17, 94%) and mucosal nodularity (4/17, 24%), accompanied by bronchial stenosis in 16 patients (94%). Paragonimus eggs were detected in the bronchial washing fluid of 9 out of the 17 patients with endobronchial lesions. The bronchial mucosal biopsy specimens showed evidence of chronic inflammation with eosinophilic infiltration in 6 out of 11 patients (55%). However, no adult fluke or ova were found in the bronchial tissue. Conclusion: Bronchial stenosis with mucosal changes including edematous swelling and mucosal nodularity is the most common bronchoscopic finding of pulmonary paragonimiasis.
S-211 Identification of a novel gene variant of SCN1B in a Korean patient with Brugada syndr ome
( Won Kyeong Jeon ),( Youngjin Cho ),( Il-young Oh ) 대한내과학회 2016 대한내과학회 추계학술대회 Vol.2016 No.1
Brugada syndrome (BS) is inheritable disease characterized by typical ECG morphologies of right bundle-branch block and ST segment elevation of right precordial leads without any structural heart disease. The change of transmembrane ion current is a major pathophysiological mechanism of BS which can lead to sudden cardiac death. Many genetic mutations of various ion channels were reported. We described here the case of a Korean patient with a novel gene variant of SCN1B first reported in the world. In May 2015, 51-year-old male transferred to emergency room of Seoul National University Bundang hospital. He was already resuscitated after five times of shock by public emergency response team from initial rhythm of ventricular fibrillation. Brain CT, coronary CT, and echocardiography was normal. While reviewing medical records, his treadmill test done 11 years ago was found out. It showed spontaneous changes on ECG, which was compatible with Brugada pattern type I. He was clinically diagnosed as BS and implantable cardiac defibrillator was inserted. Genetic analysis was performed and the result showed missense mutation of 35th codon (exon 1) in SCN1B from cytosine to adenine which resulted amino acid change from alanine to glutamic acid (SCN1B c.35C>A (p.A12E)) (Figure). This might cause anatomical or functional change in beta1-subunit of sodium channel which can cause fatal arrhythmia. According to Leiden Open Variation Database, ClinVar Database and Korean Reference Genome Database, this is a novel gene mutation never reported before.
( Won Jun Song ),( Jung Seop Eom ),( Hong Seok Yoo ),( Byeong Ho Jeong ),( Ho Yun Lee ),( Won Jung Koh ),( Kyeong Man Jeon ),( Hye Yun Park ) 대한결핵 및 호흡기학회 2014 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.118 No.-
Background: Because chronic obstructive pulmonary disease (COPD) is a heterogeneous disorder, various factors could be associated with the severity of pneumonia in patients with COPD. Therefore, we examined the risk factors associated with severe pneumonia in a COPD population. Methods: A retrospective observational study with a prospectively collected database of community-acquired pneumonia (CAP) was conducted between 2008 and 2012. Patients with hospital-acquired pneumonia, immunocompromised patients, and those without a baseline computed tomography (CT) scan were excluded. As a result, 150 patients with COPD and CAP were included. Results: Of the 150 patients, 106 (70.7%) and 44 (29.3%) were classified with non-severe and severe pneumonia, respectively. A multivariate logistic regression analysis revealed that the presence of pulmonary emphysema on a CT scan (odds ratio, 2.933; 95% confidence interval, 1.144-7.516; P = 0.025) was independently associated with severe pneumonia in patients with COPD. However, no significant difference in severity of the airflow limitation, presence of bronchiectasis, previous COPD medication including inhaled corticosteroids, or pathogens was identified between those with non-severe and severe pneumonia. Conclusions: The presence of pulmonary emphysema on a CT scan at the time of admission to the hospital of a patient with COPD was independently associated with severe pneumonia in a COPD population.
Kyeong-Min Kim,Soo-Young Yum,Song-Jeon Lee,Hee-Soo Kim,Hyeong-Jong Kim,Ji-Hyun Park,Sung-Hue Koo,Ji-Hyun Lee,Won-Wu Lee,Goo Jang,Woo-Sung Lee 한국수정란이식학회 2018 한국수정란이식학회 학술대회 Vol.2018 No.11
Interferon tau (IFNT), has known as a key signal molecule for a period of pregnancy in ruminants owing to the need on maternal recognition of pregnancy. It is generated in trophectoderm cells of the elongation bovine conceptus at day 13-21 and a peak output is at day 15-17 of pregnancy period. Moreover, other studies indicated that it can be effective in the embryonic development and quality. In previous study, there were 8 bovine IFNT, but only 2 forms of IFNTs, IFNT2 and IFN-tau-c1, were expressed by the conceptuses during the peri-implantation. In this study, we target the one between the two, IFN-tau-c1 and then the effect of IFNT knockout in donor cells to bovine cloned embryonic development by somatic cell nuclear transfer (SCNT) was investigated. In order to proceed this study, the immature oocytes from the ovaries at local slaughterhouse have been matured in vitro for 22 hours. For preparing the donor cell that have a mutation on IFNT gene, somatic cells were transiently transfected with Cas9 protein and single guide RNA targeting IFNT, and various single derived colonies with high proliferation were isolated and confirm the mutation by PCR. Finally, one colony had mono-allelic mutation (4bps deletion) was picked out and applied as the donor cell to SCNT. A donor cell was injected into an oocyte that nucleus was removed. Reconstructed oocytes with the donor cell were fused by electrical shock, activated by chemical stimulation and cultured for 7 days in chemically defined medium. In this study, control (n=199) and IFNT knockout-group (n=219) were compared with four replications. As results, there was no significant difference between control-and IFNT-knockout group not only in cleavage rate, but also blastocyst formation rate (Control: 12.3% ± 9.2, IFNT knockout-group: 20.1 ± 11%). In addition, the number of blastocyst cell was not different between control (91.7 ± 26.2) and IFNT knockout group (83.5 ± 21.3). Some IFNT mutated blastocysts from SCNT were randomly selected for confirmation of the deletion of IFNT and all samples were positive for mutation. In conclusion, these data indicated that the interruption of IFNT did not influence the embryonic development. In future study, we will transfer these mutated embryos toto test the effect of IFNT for pregnancy period. This work was supported by BK21 PLUS Program for Creative Veterinary Science, the National Research Foundation of Korea (2017R1A2B3004972) and the Technology Development Program (S2566872) by MSS.