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Su Feng,Weicai Zeng,Fan Luo,Jian Zhao,Zhirong Yang,Qun Sun 한국식품과학회 2010 Food Science and Biotechnology Vol.19 No.1
Pine needle (Pinus massoniana Lamb.) of large quantity in China and health benefit makes its application on pharmaceutical and food industry in high demand. The chemical composition of pine needle aqueous extract (PNAE) analyzed by gas chromatograph-mass spectrometry revealed that among more than 10 compounds in PNAE,organic acids were over 76.92%, with acetic acid being 25.20%, hexadecanoic acid 18.19%, and 2-methoxy-4-vinylphenol 16.44%. Ultra-performance liquid chromatography-mass spectrometry disclosed other 5 short chain organic acids, including citric acid, succinic acid, malonic acid, malic acid, and oxalic acid. The antibacterial activity of PNAE on common spoilages and pathogenic bacteria showed that the growth of Bacillus subtilis, Staphylococcus aureus, Bacillus cereus, Micrococcus luteus, Escherichia coli, and Proteus vulgaris were inhibited significantly, with minimum inhibitory concentrations and minimum bactericidal concentrations being 3.8-15 and 7.5-30 mg/mL, respectively. Our findings suggested that pine needles with effective and safe antibacterial components possess the potential to be developed into efficacious natural antiseptic products for food disinfection and medical purpose.
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Xiaoping Fan,Bohong Fan,Yuxiang Wang,Weicai Yang 한국식물생명공학회 2016 Plant biotechnology reports Vol.10 No.1
Breeding of naturally colored cotton fiber has been hampered by the limited germplasm, an alternative way is to use transgenic approach to create more germplasm for breeding. Here, we report our effort to engineer anthocyanin production in cotton. The maize Lc gene, under the control of the constitutive 35S promoter, was introduced into cotton through genetic transformation. Our data showed that the expression of the Lc gene alone is sufficient to trigger the accumulation of anthocyanin in a variety of cell types including fiber cells in cotton. However, the accumulation of colored anthocyanin in cotton fibers requires the participation of light signaling. These data indicate that it is feasible to engineer colored fibers through transgenic approach in cotton. Furthermore, we showed that the Lc-transgenic cotton plants are resistant to cotton bollworm. These transgenic plants are, therefore, potentially useful for cotton breeding against cotton bollworm.
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Jingsong He,Ni Xie,Jianbo Yang,Hong Guan,Weicai Chen,Huisheng Wu,Zishan Yuan,Kun Wang,Guojin Li,Jie Sun,Limin Yu 한국유방암학회 2014 Journal of breast cancer Vol.17 No.3
Purpose: Synuclein-γ (SNCG), which was initially identified asbreast cancer specific gene 1, is highly expressed in advancedbreast cancers, but not in normal or benign breast tissue. Thisstudy aimed to evaluate the effects of SNCG siRNA-treatmenton breast cancer cells and elucidate the associated mechanisms. Methods: Vectors containing SNCG and negative control(NC) siRNAs were transfected into MDA-MB-231 cells; mRNAlevels were determined by real-time polymerase chain reaction. Cell proliferation was evaluated using the MTT assay, cell migrationwas assessed by the Transwell assay, apoptosis and cellcycle analyses were conducted with the flow cytometer, andWestern blot analysis was performed to determine the relativelevels of AKT, ERK, p-AKT, and p-ERK expression. Results:SNCG mRNA levels were significantly reduced in MDA-MB-231cells transfected with SNCG siRNA. Our results indicate that inSNCG siRNA-treated cells, cell migration and proliferation decreasedsignificantly, apoptosis was induced, and the cell cyclewas arrested. Western blot analysis indicated that the proteinlevels of p-AKT and p-ERK were much lower in the SNCG siRNA-treated groups, than in the control and NC groups. Conclusion:SNCG siRNA could decrease the migration and proliferationof breast cancer cells by downregulating the phosphorylationof AKT and ERK.
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Chuang Jiang,Lei Wang,Xuexiang Yu,Weicai Lv,Xu Yang 대한토목학회 2022 KSCE JOURNAL OF CIVIL ENGINEERING Vol.26 No.5
3D surface deformation monitoring methods based on the current single-track Interferometric Synthetic Aperture Radar (InSAR) technology are constructed by integrating the deformation laws of mining horizontal or gently inclined coal seams in plain areas, which are not suitable for monitoring the 3D deformation of mining in mountainous areas. Therefore, we developed a new method of extracting 3D deformation of mining in mountainous areas by using single-track InSAR technology. Firstly, the Line of Sight (LOS) deformation equations were established based on geometric relations between the InSAR monitored LOS deformation and 3D surface deformation. Secondly, they were fused with basic principles of surface deformation and movement of mining in mountainous areas. Then they were solved based on relevant boundary conditions. Simulation results of this novel method showed that the accuracy values of extracted deformation along vertical section, East-West (EW) and North-South (NS) were better than 8.86 mm, 8.29 mm and 18.01 mm, respectively. Compared with Wang method, this method is suitable for surface deformation monitoring of mining subsidence in mountainous areas. Finally, the proposed method was successfully used to monitor the 3D deformation of mining in the mountainous area of Tangjiahui Coal Mine in Ordos, Inner Mongolia, China.
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