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Karyotypic Study on Some Populations of Thymus migricus Klokov & Desj.-Shost.
Alireza Yavari,Vahideh Nazeri,Fatemeh Sefidkon,Mohammad Esmail Hassani 한국원예학회 2010 Horticulture, Environment, and Biotechnology Vol.51 No.2
Five populations of Thymus migricus Klokov & Desj.-Shost., which is endemic to Flora Iranica area, were studied karyologically. Included in the data set were the basic chromosome number, the ploidy level, the total length of the haploid chromosome complement (TLC) and five different measures of karyotype asymmetry. The basic chromosome number of the studied populations was obtained as x = 14. The presence of 28 chromosomes in Heris, Nazloo and Band populations and 56 chromosomes in Jolfa and Ghushchi populations corresponds to diploidy (2n = 2x = 28) and tetraploidy (2n = 4x = 56) levels, respectively. The size of mitotic chromosomes was very small and the mean length varied from 0.69 to 1.42 μm. The smallest chromosomes were observed in Ghushchi population and the largest in Heris population. Jolfa population had the largest total chromosome length and Nazloo population had the smallest value. In all populations, metacentric chromosomes were the most common whereas sub-metacentric chromosomes were rare. Karyotypic indices obtained for five populations revealed that the karyotypes were slightly asymmetrical. A1 index varied from 0.492 to 0.643, interchromosomal asymmetry index (A₂) ranged between 0.020 and 0.102 and Paszko’s asymmetry index (AI) ranged from 0.07 to 0.20. Heris and Jolfa populations were considered to indicate higher levels of karyotypic heterogeneity.
Mohammad Fattahi,Vahideh Nazeri,Fatemeh Sefidkon,Zabihollah Zamani,Javier Palazon 한국원예학회 2011 Horticulture, Environment, and Biotechnology Vol.52 No.6
The aim of this study was to investigate the effect of pre-sowing treatments and light on Dracocephalum kotschyi Bioss. seed germination. The following treatments were used: sandpaper; 3, 5 and 10 min soaking in sulfuric acid; 15 min in sodium hypochlorite; 24 h in water, hot water and boiling water; 50, 150, and 250 mg・L^(-1) of Gibberellic acid; 1000, 2000,4000, and 6000 mg・L^(-1) of KNO3, and water (control). The germination rate, germination start (GS), mean germination time (MGT), time to 50% germination (T_(50)) and cumulative germination were determined as germination indices. Root, shoot and seedling length and the vigor index (VI) were evaluated as growth factors at the end of the incubation period. Second experiment was carried out to study the effect of light on seed germination. It was observed that both the physical and chemical scarification methods were effective in enhancing D. Kotschyi seed germination and growth. In the first stage, up to day 20 SA (10 min) and sandpaper scarification treated seed had shown germination more than 70% whereas control seeds did not begin germination until day 20. The highest germination rate was observed with sandpaper (1.77 units) and SA (1.56 units)in compare with other treatments (less than 0.9 units). Also the highest vigor index was found in SA- and sandpaper-treated seeds and the lowest occurred in sodium hypochlorite treatments. The other results showed that light was more effective than darkness for seed germination.