Antitumor effects of valdecoxib on hypopharyngeal squamous carcinoma cells

Trang Nguyen Thi Kieu,유훈 대한약리학회 2022 The Korean Journal of Physiology & Pharmacology Vol.26 No.6

The antitumoral effects of valdecoxib (Val), an United States Food and Drug Administration-approved anti-inflammatory drug that was withdrawn due to the side effects of increased risk of cardiovascular adverse events, were investigated in hypopharyngeal squamous cell carcinoma cells by performing a cell viability assay, transwell assay, immunofluorescence imaging, and Western blotting. Val markedly inhibited cell viability with an IC50 of 67.3 μM after 48 h of treatment, and also downregulated cell cycle proteins such as Cdks and their regulatory cyclin units. Cell migration and invasion were severely suppressed by inhibiting integrin α4/FAK expression. In addition, Val activated the cell cycle checkpoint CHK2 in response to excessive DNA damage, which led to the activation of caspase-3/9 and induced caspase-dependent apoptosis. Furthermore, the signaling cascades of the PI3K/AKT/ mTOR and mitogen-activated protein kinase pathways were significantly inhibited by Val treatment. Taken together, our results indicate that Val can be used for the treatment of hypopharyngeal squamous cell carcinoma.

Deep eutectic solvent-assisted synthesis of gold nanoflowers supported on glassy carbon electrode for DNA sensor application

Trang Nguyen Thi Kieu,Phuong Thao Dao Vu,Nguyet Nguyen Thi,Dien Nguyen Dac,Van Hoang Nhu,Van Thu Vu,Tran Manh Trung,Pham Hung Vuong,Tam Phuong Dinh 한국물리학회 2024 Current Applied Physics Vol.63 No.-

Gold nanoflowers (AuNF) were synthesized on a glassy carbon electrode via a one-step, eco-friendly protocol in deep eutectic solvent (DES) of choline choloride and urea, called reline, for label-free detection of DNA hybridization. DES is eco-friendly, low-cost, biocompatible, and nontoxic, and it can be used as an electrolyte to synthesize nanomaterials by using the electrochemical method. In this protocol, highly branched and stable AuNFs were obtained without using any surfactants for DNA sensor application. The electrochemical performance of the AuNF-modified electrode was studied by cyclic voltammetry and electrochemical impedance spectroscopy. Under optimal conditions, the AuNF-based DNA biosensor exhibited a sensitivity of 294.9 Ω nM−1cm−2 and 218 μA nM−1cm−2 and a limit of detection (LOD) of 10−9 M. The remarkable sensitivity and low LOD could be attributed to the good conductivity of AuNFs for accelerating electron transfer, resulting in obvious signal amplification. The DNA biosensor showed good reproducibility (RSD <3.65 %) and acceptable stability and selectivity. Its excellent performance in DNA detection suggested that the proposed electrochemical DNA sensor has great application potential in clinical diagnosis.

Antitumor effects of ophiopogonin D on oral squamous cell carcinoma

Nguyen Thi Kieu Trang,Vu Phuong Dong,유훈 대한구강생물학회 2024 International Journal of Oral Biology Vol.49 No.2

Ophiopogonin D (OPD) is a steroidal glycoside derived from Ophiopogon japonicus , a traditional Chinese medicine with diverse biological activities, including antithrombosis, anti-inflammation, and antitussive effects. To investigate the cellular effects and mechanisms of OPD on oral squamous cell carcinoma, cell viability was explored, and the effects of OPD on cell cycle regulators, apoptotic marker proteins, and key proteins involved in metastasis and signaling pathways were examined by MTT assay and Western blotting in YD38 cells. OPD strongly inhibited cell proliferation and induced caspase-dependent apoptosis of YD38 cells by suppressing the cell cycle and activating caspase-3 and poly ADP ribose polymerase. Additionally, OPD suppressed the expression of vital proteins regulating metastasis and proliferation within the integrin/matrix metalloproteinases/FAK and AKT/PI3K/mTor pathways. Thus, OPD can be a potential treatment candidate for gingival cancer.

Risk of Material Misstatement in the Stage of Audit Planning: Empirical Evidence from Vietnamese Listed Enterprises

NGUYEN, Hoan,NGO, Thi Kieu Trang,LE, Thi Tam Korea Distribution Science Association 2020 The Journal of Asian Finance, Economics and Busine Vol.7 No.3

The purpose of this study is to investigate factors influencing risk assessment of material misstatement in Vietnamese enterprises listed on stock market. Expert interview method was conducted to discover the scales for three variables including information system, trademark, and risk assessment of material misstatement. Survey method was used to examine the impacts of eight factors on risk assessment of material misstatement. Data is collected from 317 auditors who have excellent experience in auditing financial statements of companies listed on stock market. Then, data is processed by descriptive statistics, reliability analysis, factor extracted analysis, correlative regression analysis, and analysis variance of residual change. The research findings showed that business characteristic, stakeholder pressure, and economic environment have positive relationships with risk assessment of material misstatement. Three variables including operation control and monitor, control environment, and information system negatively affect to risk assessment. Specially, business characteristic and information system, which are elements in internal control, have strongest impact on risk assessment. One the other hand, assessment of internal control plays an important role not only in the audit plan stage but also throughout the stages of the audit implementation and ending. Therefore, appropriate solutions are proposed to carry out all audit stages.

Tannic acid-induced apoptosis in FaDu hypopharyngeal squamous cell carcinoma

Loan Thi Ta,Trang Thi Kieu Nguyen,Hoon Yoo 대한구강생물학회 2019 International Journal of Oral Biology Vol.44 No.2

Tannic acid (TA) is a water-soluble polyphenol compound found in various herbal plants. We investigated the chemopreventive effects of TA on FaDu hypopharyngeal squamous carcinoma cells. In an 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, TA showed dose-dependent cytotoxicity with a half maximal inhibitory concentration (IC50) of 50 µM. Cell cycle analysis and immunofluorescence imaging demonstrated that under low-dose (25 µM) treatment, FaDu cells were arrested in G2/M phase, and as the dose of TA was increased, apoptosis was induced with the increase of cell population at sub-G1 phase. The expressions of various cyclins, including cyclin D1 and cyclin-dependent kinases (CDK-1 and CDK-2), were down-regulated at low doses of TA, whereas apoptotic effectors such as cleaved caspase 3, cleaved caspase 7, and poly (ADP-ribose) polymerase (PARP) were expressed in a dose-dependent manner in Western blotting. In addition, TA-induced apoptosis of FaDu cells might be mediated by the extracellular signal-regulated kinase (ERK)/mitogen-activated protein kinase pathway, with the upregulation of p-AKT/p-PKB (phosphorylated protein kinase B) and p-ERK. Overall, our data support the hypothesis that TA is a potential candidate agent for the treatment of hypopharyngeal cancer.

Factors Affecting Entrepreneurial Intention of Generation Z During COVID-19 Pandemic: An Empirical Study from Vietnam

Trong Luan NGUYEN,Tran Gia Thanh LE,Bach Mai HUYNH,Thi Kieu Trang VO,Pham Hai Au HA 한국유통과학회 2021 The Journal of Asian Finance, Economics and Busine Vol.8 No.12

Gen Z is a special generation that was born with technology, converging the best development conditions making them an important part of the future development of Vietnam’s economy. Gen Z is an important part of the future development of Vietnam’s economy. The purpose of this study is to identify and measure the impact of various factors affecting the business intentions of Gen Z in Vietnam during the Covid pandemic. The study did not go into the details of entrepreneurship, instead focused on explaining the impact of factors such as risk-taking, market economic trends, influencers, confidence, and family business traditions on the business intentions of Gen Z. The study was carried out using quantitative and qualitative methods with 335 data points collected online via survey links. The methods used to test the scale such as Cronbach alpha, CFA, SEM are used to examine the correlation between factors affecting the business intention of Gen Z in Vietnam. The results showed that the business intention of Gen Z in Vietnam is positively correlated and significantly influenced by confidence and risk-taking. Furthermore, the study reveals a difference in entrepreneurship of Gen Z Vietnam based on gender and education level.

Approximate-SAD Circuit for Power-efficient H.264 Video Encoding under Maintaining Output Quality and Compression Efficiency

Le Dinh Trang Dang,Nguyen Thi My Kieu,Ik Joon Chang,Jinsang Kim 대한전자공학회 2016 Journal of semiconductor technology and science Vol.16 No.5

We develop a novel SAD circuit for power-efficient H.264 encoding, namely a-SAD. Here, some highest-order MSB’s are approximated to single MSB. Our theoretical estimations show that our proposed design simultaneously improves performance and power of SAD circuit, achieving good power efficiency. We decide that the optimal number of approximated MSB’s is four under 8-bit YUV-420 format, the largest number not to affect video quality and compression-rate in our video experiments. In logic simulations, our a-SAD circuit shows at least 9.3% smaller critical-path delay compared to existing SAD circuits. We compare power dissipation under iso-throughput scenario, where our a-SAD circuit obtains at least 11.6% power saving compared to other designs. We perform same simulations under two-and three-stage pipelined architecture. Here, our a-SAD circuit delivers significant performance (by 13%) and power (by 17% and 15.8% for two and three stages respectively) improvements.

Preparation and characterization of rutile phase TiO2 nanoparticles and their cytocompatibility with oral cancer cells

Vu Phuong Dong,Nguyen Thi Kieu Trang,Hoon Yoo 대한구강생물학회 2019 International Journal of Oral Biology Vol.44 No.3

In the present study, rutile phase titanium dioxide nanoparticles (R-TiO 2 NPs) were prepared by hydrolysis of titanium tetrachloride in an aqueous solution followed by calcination at 900℃. The composition of R-TiO 2 NPs was determined by the analysis of X-ray diffraction data, and the characteristic features of R-TiO 2 NPs such as the surface functional group, particle size, shape, surface topography, and morphological behavior were analyzed by Fourier-transform infrared spectroscopy, scanning electron microscopy and energy dispersive X-ray spectroscopy, transmission electron microscopy, dynamic light scattering, and zeta potential measurements. The average size of the prepared R-TiO2 NPs was 76 nm, the surface area was 19 m 2 /g, zeta potential was −20.8 mV, and average hydrodynamic diameter in dimethyl sulfoxide (DMSO)–H 2 O solution was 550 nm. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and morphological observations revealed that R-TiO 2 NPs were cytocompatible with oral cancer cells, with no inhibition of cell growth and proliferation. This suggests the efficacy of R-TiO 2 NPs for the aesthetic white pigmentation of teeth.

The Southern World as a Destination of International Students: an Analysis of 50 Tertiary Education Institutions in Vietnam

Pham, Hiep-Hung,Vuong, Quan-Hoang,Dong, Thi-Kieu-Trang,Nguyen, Tien-Trung,Ho, Manh-Toan,Vuong, Thu-Trang,Hoang, Anh-Duc,Nguyen, Mai-Huong World Association for Triple Helix and Future Stra 2021 Journal of Contemporary Eastern Asia Vol.20 No.1

Traditionally, students from the Southern world tend to cross their national borders to study abroad. However, in recent times, we have observed a trend in which more and more students, both full-time and short-term, select Southern countries as destinations for overseas education. This paper contributes to the sparse literature on the above phenomenon, examining the case of Vietnam. We surveyed 50 universities and colleges about their international student profiles (including statistics and nationalities) and their strategies for attracting international students. The findings of this study provide implications for Vietnam's policymakers and university/college leaders and other Southern countries when implementing the internationalization of tertiary education.