Antihepatotoxic Activity of Cassia tora Leaf Extract

Tapan K. Maity,Subhash C. Mandal,M. Pal,B. P. Saha 한국생약학회 1998 Natural Product Sciences Vol.4 No.4

Methanolic extract of Cassia tora leaves was evaluated for its hepatoprotective activity in rats by inducing hepatotoxicity with paracetamol (acute model). The extract at a dose of 400 ㎎/㎏ orally exhibited significant protective effect by lowering the serum levels of transaminase (SGOT and SGPT), bilirubin, and alkaline phosphatase (ALP). The effects produced were comparable to that of a standard hepatoprotective agent.

Evaluation of Hepatoprotective Potential of Cassia tora Leaf Extract

Maity, Tapan Kumar,Mandal, Subhash C.,Mukherjee, Pulok K.,Saha, Kakali,Das, J.,Saha, B.P.,Pal, M. The Korean Society of Pharmacognosy 1997 Natural Product Sciences Vol.3 No.2

Methanolic extract of the leaves of Cassia tom was evaluated for its hepatoprotective potentials in rats by inducing hepatotoxicity with carbon tetrachloride. The extract at a dose of 400 mg/kg orally showed significant protective effect by lowering the serum levels of transaminase (SGOT and SGPT), bilirubin and alkaline phosphatase (ALP). The effects produced were comparable to that of a standard hepatoprotective agent.

A study on wound healing activity of Bacopa monnieri Linn. aerial parts

Ghosh, Tirtha,Maity, Tapan Kumar,Dash, Deepak Kumar,Boss, Anindya Kyung Hee Oriental Medicine Research Center 2007 Oriental pharmacy and experimental medicine Vol.7 No.2

Bacopa monnieri is being used in the traditional system of medicine for a variety of ailments. In the present study, the ethanolic extract of Bacopa monnieri aerial parts has been studied for its wound healing activity using various models in rats. Significant increase in wound contraction and skin breaking strength were observed in the excision and incision wound models respectively. There was also significant increase in hydroxyproline content, DNA content, superoxide dismutase activity, catalase activity, glutathione level and decrease in the level of thiobarbituric acid reactive substances in the $12^{th}$ day post wounding tissue of experimental rats in the ethanol extract treated groups with respect to the control group. The effect of the extract was found to be comparable with the standard drug nitrofurazone. From the results it may be concluded that the plant Bacopa monnieri is endowed with significant wound healing activity, thereby justifying its use in the traditional medicine. Saponins may be responsible for the observed wound healing activity.

Effect of various fractions of Bacopa monnieri Linn. aerial parts on ethanol-induced hepatotoxicity in rats

Ghosh, Tirtha,Maity, Tapan Kumar,Dash, Deepak Kumar,Bose, Anindya Kyung Hee Oriental Medicine Research Center 2007 Oriental pharmacy and experimental medicine Vol.7 No.3

The ethyl acetate fraction (EAF) and n-butanol fraction (NBF) of ethanolic extract of Bacopa monnieri aerial parts were screened for hepatoprotective activity and in vivo antioxidant activity on ethanol-induced hepatotoxic rats. Ethyl acetate fraction was found to be more potent even though both the fractions were endowed with significant hepatoprotective activity. EAF and NBF were investigated for hepatoprotective activity in albino rats at 300 mg/kg, p.o. dose and compared with standard drug Silymarin (25 mg/kg, p.o.). Results show that both the fractions were effective in blunting ethanol-induced enhanced activities of serum glutamate oxaloacetate transaminase, serum glutamate pyruvate transaminase, alkaline phosphatase, level of serum bilirubin (both total and direct), liver weight loss and was also effective in reducing ethanol-induced lipid peroxidation both in vitro and in vivo. Furthermore, the fractions could also enhance ethanol-induced suppressed activities of superoxide dismutase, catalase and decreased level of reduced glutathione. Results of hepatocellular damage caused by ethanol and its recovery by EAF and NBF, suggest that they might be considered as a potential source of natural hepatoprotective agents, which could be related to the free radical scavenging properties of saponins present in high concentration in the fractions.

A study on wound healing activity of Bacopa monnieri Linn. aerial parts

Tirtha Ghosh,Tapan Kumar Maity,Deepak Kumar Dash,Anindya Bose 경희대학교 융합한의과학연구소 2007 Oriental Pharmacy and Experimental Medicine Vol.7 No.2

Bacopa monnieri is being used in the traditional system of medicine for a variety of ailments. In the present study, the ethanolic extract of Bacopa monnieri aerial parts has been studied for its wound healing activity using various models in rats. Significant increase in wound contraction and skin breaking strength were observed in the excision and incision wound models respectively. There was also significant increase in hydroxyproline content, DNA content, superoxide dismutase activity, catalase activity, glutathione level and decrease in the level of thiobarbituric acid reactive substances in the 12th day post wounding tissue of experimental rats in the ethanol extract treated groups with respect to the control group. The effect of the extract was found to be comparable with the standard drug nitrofurazone. From the results it may be concluded that the plant Bacopa monnieri is endowed with significant wound healing activity, thereby justifying its use in the traditional medicine. Saponins may be responsible for the observed wound healing activity.

Evaluation of antitumor activity of stigmasterol, a constituent isolated from Bacopa monnieri Linn aerial parts against Ehrlich Ascites Carcinoma in mice

Tirtha Ghosh,Tapan Kumar Maity,Jagadish Singh 경희대학교 융합한의과학연구소 2011 Oriental Pharmacy and Experimental Medicine Vol.11 No.1

Cancer remains as one of the most common cause of mortality worldwide. Cancer chemoprevention by natural products is well accepted nowadays. Phytoserols are natural products, showing anticancer activity, besides other activities. The purpose of this study was to examine the antitumor and antioxidant activity of stigmasterol, a phytosterol isolated from aerial parts of Bacopa monnieri Linn. against Ehrlich Ascites Carcinoma (EAC) in swiss albino mice. The effect of stigmasterol on the growth of transplantable murine tumor, life span of EAC bearing hosts, simultaneous alterations in the hematological profile,liver biochemical parameters (lipid peroxidation, antioxidant enzymes) and histopthological studies of liver were examined. Stigmasterol decreased tumor volume, packed cell volume and viable cell count, and increased mean survival time thereby increasing life span of EAC tumor bearing mice. Hematological profile reverted to near normal levels in stigmasterol treated mice. Stigmasterol decreased the levels of lipid peroxidation and increased the levels of glutathione, superoxide dismutase and catalase in the liver of EAC bearing mice. Histopathological study of liver tissues showed that stigmasterol had significant protective effect against EAC bearing mice, which corroborates the above findings. Lactate dehydrogenase (LDH) activity in ascitic fluid increased while membrane microviscosity of the EAC cells decreased upon stigmasterol treatment indicating its effect in the membrane integrity functions. The antitumor activity of stigmasterol might be mediated through the activation of protein phosphatase 2A by ceramide causing apoptosis, as is shown by structurally similar phytosterol.

Hepatoprotective Activity of Ethanolic Extract of Bacopa monnieri Linn. AerialParts Against CCl4-induced Hepatotoxicity in Rats

Tirtha Ghosh,Tapan Kumar Maity,Gouri Kumar Dash,Anindya Bose,Siva Sankar Nayak,Deepak Kumar Dash 한국생약학회 2007 Natural Product Sciences Vol.13 No.1

ethanolic extract of Bacopa monnieri Linn. aerial parts were studied for its hepatoprotective efecton CCl4-induced hepatotoxic rats. The extract was found to decrease significantly CCl4-induced elevation ofSGOT, SGPT, ALP, bilirubin and total cholesterol. But, it increased HDL-cholesterol level and liver weight withrespect to CCl4 toxic rats. The extract was also found to decrease significantly the CCl4-induced elevation of lipidperoxidation and increase the activity of antioxidant enzymes (SOD and CAT) and GSH level in the liver ofextract treated rats when compared with CCl4 induced rats. Histopathological profiles showed that the extract hadsignificant protective effect against CCl4may be possible that the mechanism of hepatoprotection of the extract is due to its antioxidant effect.KeywordsBacopa monnieri L., ethanolic extract, hepatoprotective activity, antioxidant activity, histopathology.

Evaluation of antitumor activity of stigmasterol, a constituent isolated from $Bacopa$ $monnieri$ Linn aerial parts against Ehrlich Ascites Carcinoma in mice

Ghosh, Tirtha,Maity, Tapan Kumar,Singh, Jagadish 경희한의학연구센터 2011 Oriental Pharmacy and Experimental Medicine Vol.11 No.1

Cancer remains as one of the most common cause of mortality worldwide. Cancer chemoprevention by natural products is well accepted nowadays. Phytoserols are natural products, showing anticancer activity, besides other activities. The purpose of this study was to examine the antitumor and antioxidant activity of stigmasterol, a phytosterol isolated from aerial parts of $Bacopa$ $monnieri$ Linn. against Ehrlich Ascites Carcinoma (EAC) in swiss albino mice. The effect of stigmasterol on the growth of transplantable murine tumor, life span of EAC bearing hosts, simultaneous alterations in the hematological profile, liver biochemical parameters (lipid peroxidation, antioxidant enzymes) and histopthological studies of liver were examined. Stigmasterol decreased tumor volume, packed cell volume and viable cell count, and increased mean survival time thereby increasing life span of EAC tumor bearing mice. Hematological profile reverted to near normal levels in stigmasterol treated mice. Stigmasterol decreased the levels of lipid peroxidation and increased the levels of glutathione, superoxide dismutase and catalase in the liver of EAC bearing mice. Histopathological study of liver tissues showed that stigmasterol had significant protective effect against EAC bearing mice, which corroborates the above findings. Lactate dehydrogenase (LDH) activity in ascitic fluid increased while membrane microviscosity of the EAC cells decreased upon stigmasterol treatment indicating its effect in the membrane integrity functions. The antitumor activity of stigmasterol might be mediated through the activation of protein phosphatase 2A by ceramide causing apoptosis, as is shown by structurally similar phytosterol.

Effect of various fractions of Bacopa monnieri Linn. aerial parts on ethanolinduced hepatotoxicity in rats

Tirtha Ghosh,Tapan Kumar Maity,Deepak Kumar Dash,Anindya Bose 경희대학교 융합한의과학연구소 2007 Oriental Pharmacy and Experimental Medicine Vol.7 No.3

The ethyl acetate fraction (EAF) and n-butanol fraction (NBF) of ethanolic extract of Bacopa monnieri aerial parts were screened for hepatoprotective activity and in vivo antioxidant activity on ethanolinduced hepatotoxic rats. Ethyl acetate fraction was found to be more potent even though both the fractions were endowed with significant hepatoprotective activity. EAF and NBF were investigated for hepatoprotective activity in albino rats at 300 mg/kg, p.o. dose and compared with standard drug Silymarin (25 mg/kg, p.o.). Results show that both the fractions were effective in blunting ethanolinduced enhanced activities of serum glutamate oxaloacetate transaminase, serum glutamate pyruvate transaminase, alkaline phosphatase, level of serum bilirubin (both total and direct), liver weight loss and was also effective in reducing ethanol–induced lipid peroxidation both in vitro and in vivo. Furthermore, the fractions could also enhance ethanol-induced suppressed activities of superoxide dismutase, catalase and decreased level of reduced glutathione. Results of hepatocellular damage caused by ethanol and its recovery by EAF and NBF, suggest that they might be considered as a potential source of natural hepatoprotective agents, which could be related to the free radical scavenging properties of saponins present in high concentration in the fractions.

Ficus racemosa Affords Antihepatotoxic Activity Against Paracetamol-Induced Acute Liver Damage in Rats

Subhash C. Mandal,Tapan K. Maity,J. Das,B. P. Saha,M. Pal 한국생약학회 1998 Natural Product Sciences Vol.4 No.3

The effect of oral treatment with Ficus racemosa leaf extract (400 ㎎/㎏ for seven days) were studied on hepatic damage induced by paracetamol (750 ㎎/㎏, i.p.) in rats. Biochemical parameter like SGOT, SGPT, serum bilirubin and alkaline phosphatase were estimated to assess liver function. These biochemical observations were supplemented by histopatllalogical examination of liver sections. The activity of extract was also comparable to Neutrosec a known hepatoprotective formulation.