Bioprospecting of Novel and Bioactive Metabolites from Endophytic Fungi Isolated from Rubber Tree Ficus elastica Leaves

( Zhuang Ding ),( Tao Tao ),( Lili Wang ),( Yanna Zhao ),( Huiming Huang ),( Demeng Zhang ),( Min Liu ),( Zhengping Wang ),( Jun Han ) 한국미생물생명공학회(구 한국산업미생물학회) 2019 Journal of microbiology and biotechnology Vol.29 No.5

Endophytic fungi are an important component of plant microbiota, and have the excellent capacity for producing a broad variety of bioactive metabolites. These bioactive metabolites not only affect the survival of the host plant, but also provide valuable lead compounds for novel drug discovery. In this study, forty-two endophytic filamentous fungi were isolated from Ficus elastica leaves, and further identified as seven individual taxa by ITS-rDNA sequencing. The antimicrobial activity of these endophytic fungi was evaluated against five pathogenic microorganisms. Two strains, Fes1711 (Penicillium funiculosum) and Fes1712 (Trichoderma harzianum), displayed broad-spectrum bioactivities. Our following study emphasizes the isolation, identification and bioactivity testing of chemical metabolites produced by T. harzianum Fes1712. Two new isocoumarin derivatives (1 and 2), together with three known compounds (3-5) were isolated, and their structures were elucidated using NMR and MS. Compounds 1 and 2 exhibited inhibitory activity against Escherichia coli. Our findings reveal that endophytic fungi from the rubber tree F. elastica leaves exhibit unique characteristics and are potential producers of novel natural bioactive products.

Expression Characteristics of Proteins of the Insulin-like Growth Factor Axis in Non-small Cell Lung Cancer Patients with Preexisting Type 2 Diabetes Mellitus

Ding, Jing,Tang, Jie,Chen, Xin,Men, Hai-Tao,Luo, Wu-Xia,Du, Yang,Ge, Jun,Li, Cong,Chen, Ye,Cheng, Ke,Qiu, Meng,Liu, Ji-Yan Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.10

Background: Preexisting type 2 diabetes mellitus (T2DM) affects the prognosis and mortality of patients with some cancers. Insulin like growth factor (IGF) and insulin receptor (IR) signaling axes play important roles in both cancer and diabetes development. We aimed to explore the expression characteristics of proteins in IGF/IR axis in non-small cell lung cancer (NSCLC) cases with preexisting T2DM. Methods: Fifty-five NSCLC patients with preexisting T2DM were retrospectively included and matched by 55 NSCLC without diabetes at a 1:1 ratio. The expression of proteins in IGF/IR axis was detected by immunohistochemical staining. Clinicopathological data were collected to analyze their relationship with the protein expression. Results: Both IGF 1 receptor (IGF-1R) and insulin receptor substrate 2 (IRS-2) showed higher expression in the NSCLC with T2DM group, compared with those without T2DM. The high expression of IGF-1R and IRS-2 were found to be negatively associated with lymph node metastases and T staging in the T2DM group, respectively, and IRS-2 expression was also found more in the subgroup whose T2DM duration was more than 4 years. No difference was detected in the expression of IRS-1, IGF-1, IGF-2, IGFBP3, IR and mTOR between groups with or without T2DM. Conclusion: Our study found higher expression of IGF-1R and IRS-2 proteins in NSCLC patients with preexisting T2DM, and that there was an association with early stage NSCLC, which suggested that IGF signaling may play an important early event in development of NSCLC associated with diabetes.

Experimental and numerical investigation on the behavior of concrete-filled rectangular steel tubes under bending

Tao Zhang,Yong-zhi Gong,Fa-xing Ding,Xue-mei Liu,Zhi-wu Yu 국제구조공학회 2021 Structural Engineering and Mechanics, An Int'l Jou Vol.78 No.3

Pure bending loading conditions are not frequently occurred in practical engineering, but the flexural researches are important since it’s the basis of mechanical property researches under complex loading. Hence, the objective of this paper is to investigate the flexural behavior of concrete-filled rectangular steel tube (CFRT) through combined experimental and numerical studies. Flexural tests were conducted to investigate the mechanical performance of CFRT under bending. The load vs. deflection curves during the loading process was analyzed in detail. All the specimens behaved in a very ductile manner. Besides, based on the experimental result, the composite action between the steel tube and core concrete was studies and examined. Furthermore, the feasibility and accuracy of the numerical method was verified by comparing the computed results with experimental observations. The full curves analysis on the moment vs. curvature curves was further conducted, where the development of the stress and strain redistribution in the steel tube and core concrete was clarified comprehensively. It should be noted that there existed bond slip between the core concrete and steel tube during the loading process. And then, an extensive parametric study, including the steel strength, concrete strength, steel ratio and aspect ratio, was performed. Finally, design formula to calculate the ultimate moment and flexural stiffness of CFRTs were presented. The predicted results showed satisfactory agreement with the experimental and FE results. Additionally, the difference between the experimental/FE and predicted results using the related design codes were illustrated.

Nitrogen Fixation by Ru Single-Atom Electrocatalytic Reduction

Tao, Hengcong,Choi, Changhyeok,Ding, Liang-Xin,Jiang, Zheng,Han, Zishan,Jia, Mingwen,Fan, Qun,Gao, Yunnan,Wang, Haihui,Robertson, Alex W.,Hong, Song,Jung, Yousung,Liu, Shizhen,Sun, Zhenyu Elsevier 2019 Chem Vol.5 No.1

Genistein attenuates isoflurane-induced neurotoxicity and improves impaired spatial learning and memory by regulating cAMP/CREB and BDNF-TrkB-PI3K/Akt signaling

Tao Jiang,Xiu-qin Wang,Chuan Ding,Xue-lian Du 대한약리학회 2017 The Korean Journal of Physiology & Pharmacology Vol.21 No.6

Anesthetics are used extensively in surgeries and related procedures to prevent pain. However, there is some concern regarding neuronal degeneration and cognitive deficits arising from regular anesthetic exposure. Recent studies have indicated that brain-derived neurotrophic factor (BDNF) and cyclic AMP response element-binding protein (CREB) are involved in learning and memory processes. Genistein, a plant-derived isoflavone, has been shown to exhibit neuroprotective effects. The present study was performed to examine the protective effect of genistein against isoflurane-induced neurotoxicity in rats. Neonatal rats were exposed to isoflurane (0.75%, 6 hours) on postnatal day 7 (P7). Separate groups of rat pups were orally administered genistein at doses of 20, 40, or 80 mg/kg body weight from P3 to P15 and then exposed to isoflurane anesthesia on P7. Neuronal apoptosis was detected by TUNEL assay and FluoroJade B staining following isoflurane exposure. Genistein significantly reduced apoptosis in the hippocampus, reduced the expression of proapoptotic factors (Bad, Bax, and cleaved caspase-3), and increased the expression of Bcl-2 and Bcl-xL. RT-PCR analysis revealed enhanced BDNF and TrkB mRNA levels. Genistein effectively upregulated cAMP levels and phosphorylation of CREB and TrkB, leading to activation of cAMP/CREB-BDNF-TrkB signaling. PI3K/Akt signaling was also significantly activated. Genistein administration improved general behavior and enhanced learning and memory in the rats. These observations suggest that genistein exerts neuroprotective effects by suppressing isoflurane-induced neuronal apoptosis and by activating cAMP/CREB-BDNF-TrkB-PI3/Akt signaling.

Design of a Modular Green Closed Internal Cooling Turning Tool for Applications

Tao Wu,Tianjian Li,Xiaohong Ding,Hong Chen,Lei Wang 한국정밀공학회 2018 International Journal of Precision Engineering and Vol.5 No.2

Green machining has been aggressively pursued in the manufacturing industry in recent years. Dry or clean cutting without the hazards of cooling liquid, and their abilities to monitor thermal impact on turning tools in real time have been very appealed to the manufacturing industries. In this study, a modular green closed internal cooling turning tool is presented, in which coolant channel is designed only between an insert and an adaptor. The insert is grouped with the adaptor and designed as a Plug-and-Play component in cooling circuit. Thermal-fluid-structural coupling analysis is employed to evaluate the effects of the internal cooling system with machining process constraints. The numerical simulation results demonstrate that the newly designed internal cooing turning tool could effectively decrease the tool temperature. Preliminary experiments have further proved the effectiveness of the modular green closed internal cooling turning tool system. The study has potentially great significance in improving tooling performance, as well as achieving environmental friendly, economical and sustainable machining.

Variants on ESR1 and their Association with Prostate Cancer Risk: A Meta-analysis

Ding, Xiang,Cui, Feng-Mei,Xu, Song-Tao,Pu, Jin-Xian,Huang, Yu-Hua,Zhang, Jiang-Lei,Wei, Xue-Dong,Hou, Jian-Quan,Yan, Chun-Yin Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.8

Background: Epidemiological studies evaluating the association of two variants rs9340799 and rs2234693 on estrogen receptor 1 (ESR1) with prostate risk have generated inconsistent results. Methods: A meta-analysis was here conducted to systematically evaluate the relationship of these two variants with prostate cancer susceptibility. Results: For rs9340799, heterozygosity of T/C carriers showed a significant increased prostate cancer risk with a pooled odds ratio (OR) of 1.34 (95% CI = 1.06-1.69) while homozygote C/C carriers showed an increased but not statistically significant association with prostate cancer risk (pooled OR = 1.29, 95% CI = 0.94-1.79). Compared to the homozygous TT carriers, the allele C carriers showed a 31% increased risk for prostate cancer (pooled OR = 1.31, 95% CI = 1.06-1.63). No significant association between the rs2234693 and prostate cancer risk was found with the pooled OR of 1.15 (95% CI = 0.97-1.39, T/C and C/C vs. T/T) under the dominant genetic model. Compared to the homozygote T/T carriers, the heterozygous T/C carriers did not show any significantly different risk of prostate cancer (pooled OR = 1.13, 95% CI = 0.94-1.36) and the homozygous C/C carriers also did not show a significant change for prostate cancer risk compared to the wide-type T/T carriers (pooled OR = 1.26, 95% CI = 0.98-1.62). Conclusion: These data suggested that variant rs9340799, but not rs2234693, on ESR1 confers an elevated risk of prostate cancer.

Behavior of polygonal concrete-filled steel tubular stub columns under axial loading

Tao Zhang,Fa-Xing Ding,Liping Wang,Xue-mei Liu,Guo-shuai Jiang 국제구조공학회 2018 Steel and Composite Structures, An International J Vol.28 No.5

The objective of this paper is to investigate the mechanical performances of polygonal concrete-filled circular steel tubular (CFT) stub columns under axial loading through combined experimental and numerical study. A total of 32 specimens were designed to investigate the effect of the concrete strength and steel ratio on the compressive behavior of polygonal CFT stub columns. The ultimate bearing capacity, ductility and confinement effect were analyzed based on the experimental results and the failure modes were discussed in detail. Besides, ABAQUS was adopted to establish the three dimensional FE model. The composite action between the core concrete and steel tube was further discussed and clarified. It was found that the behavior of CFT stub column changes with the change of the cross-section, and the change is continuous. Finally, based on both experimental and numerical results, a unified formula was developed to estimate the ultimate bearing capacity of polygonal CFT stub columns according to the superposition principle with rational simplification. The predicted results showed satisfactory agreement with both experimental and FE results.

Transitional Zone Index and Intravesical Prostatic Protrusion in Benign Prostatic Hyperplasia Patients: Correlations according to Treatment Received and Other Clinical Data

Tao Huang,Jun Qi,YongJiang Yu,Ding Xu,Yang Jiao,Jian Kang,YunKai Zhu,YaQing Chen 대한비뇨의학회 2012 Investigative and Clinical Urology Vol.53 No.4

Purpose: The aim of this research was to assess the value of the transitional zone index (TZI) and intravesical prostatic protrusion (IPP) from transrectal ultrasonography in evaluating the severity and progression of disease by analyzing the relationship between the 2 parameters and symptoms, clinical history, and urodynamics in benign prostatic hyperplasia (BPH) patients undergoing different treatment. Materials and Methods: A total of 203 patients receiving medication and 162 patients who underwent transurethral resection of the prostate because of BPH were enrolled in this retrospective analysis. The clinical history and subjective and objective examination results of all patients were recorded and compared after being classified by TZI and IPP level. Linear regression was used to find correlations between IPP, TZI, and urodynamics. Results: The 2 parameters were found to differ significantly between patients receiving medication and patients undergoing surgical therapy (p<0.05). PSA, maximum flow rate (Qmax), detrusor pressure at Qmax (PdetQmax), and the bladder outlet obstruction index (BOOI) differed according to various TZI levels (p<0.05). In addition, the voiding symptom score, Qmax, and BOOI of subgroups with various IPP levels were also significantly different (p<0.05). Both TZI and IPP had significant effects on Qmax, BOOI, and PdetQmax (p<0.05) and the incidence of acute urinary retention (p=0.000). Conclusions: The results demonstrated that both TZI and IPP had favorable value for assessing severity and progression in patients with BPH. Further studies are needed to confirm whether the two parameters have predictive value in the efficacy of BPH treatment and could be considered as factors in the selection of therapy. Purpose: The aim of this research was to assess the value of the transitional zone index (TZI) and intravesical prostatic protrusion (IPP) from transrectal ultrasonography in evaluating the severity and progression of disease by analyzing the relationship between the 2 parameters and symptoms, clinical history, and urodynamics in benign prostatic hyperplasia (BPH) patients undergoing different treatment. Materials and Methods: A total of 203 patients receiving medication and 162 patients who underwent transurethral resection of the prostate because of BPH were enrolled in this retrospective analysis. The clinical history and subjective and objective examination results of all patients were recorded and compared after being classified by TZI and IPP level. Linear regression was used to find correlations between IPP, TZI, and urodynamics. Results: The 2 parameters were found to differ significantly between patients receiving medication and patients undergoing surgical therapy (p<0.05). PSA, maximum flow rate (Qmax), detrusor pressure at Qmax (PdetQmax), and the bladder outlet obstruction index (BOOI) differed according to various TZI levels (p<0.05). In addition, the voiding symptom score, Qmax, and BOOI of subgroups with various IPP levels were also significantly different (p<0.05). Both TZI and IPP had significant effects on Qmax, BOOI, and PdetQmax (p<0.05) and the incidence of acute urinary retention (p=0.000). Conclusions: The results demonstrated that both TZI and IPP had favorable value for assessing severity and progression in patients with BPH. Further studies are needed to confirm whether the two parameters have predictive value in the efficacy of BPH treatment and could be considered as factors in the selection of therapy.

The Influence of Changing PV Array Interconnections under a Non-uniform Irradiance

Ding, Kun,Feng, Li,Qin, Si-Yu,Mao, Jing,Zhang, Jing-Wei,Wang, Xiang,Peng, Tao,Zhai, Quan-Xin The Korean Institute of Power Electronics 2016 JOURNAL OF POWER ELECTRONICS Vol.16 No.2

Usually, the output characteristics of a photovoltaic (PV) array are significantly affected by non-uniform irradiance which is caused by ambient obstacles, clouds, orientations, tilts, etc. Some local maximum power points (LMPP) in the current-voltage (I-V) curves of a PV array can result in power losses of the array. However, the output power at the global maximum power point (GMPP) is different in different interconnection schemes in a PV array. Therefore, based on the theoretical analysis and mathematical derivation of different topological structures of a PV array, this paper investigated the output characteristics of dual series PV arrays with different interconnections. The proposed mathematical models were also validated by experimental results. Finally, this paper also concluded that in terms of performance, the total cross tied (TCT) interconnection was not always the optimal structure, especially in a dual series PV array. When one of the PV modules was severely mismatched, the TCT worked worse than the series parallel (SP) structure. This research can provide guidance for switching the interconnection to gain the greatest energy yield in a changeable- structure PV system.