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Ohsaki, H.,Okada, M.,Sasazaki, S.,Hinenoya, T.,Sawa, T.,Iwanaga, S.,Tsuruta, H.,Mukai, F.,Mannen, H. Asian Australasian Association of Animal Productio 2007 Animal Bioscience Vol.20 No.5
Differences of meat qualities between Japanese Black and Holstein have been known in Japan, however, the causative proteins and/or the genetic background have been unclear. The aim of this study was to identify candidate proteins causing differences of the meat qualities between the two breeds. Using technique of two-dimensional gel electrophoresis, protein profiling was compared from samples of the longissimus dorsi muscle and subcutaneous adipose tissue. Five protein spots were observed with different expression levels between breeds. By using LC-MS/MS analysis and Mascot program, three of them were identified as ankyrin repeat protein 2, phosphoylated myosin light chain 2 and mimecan protein. Subsequently, we compared the DNA coding sequences of three proteins between breeds to find any nucleotide substitution. However, there was no notable mutation which could affect pI or molecular mass of the proteins. The identified proteins may be responsible for different characteristics of the meat qualities between Japanese Black and Holstein cattle.
Study of Neutron Capture Reactions Using the 4π Ge Spectrometer
H. Harada,S. Goko,A. Kimura,M. Ohta,M. Oshima,F. Kitatani,M. Igashira,T. Katabuchi,M. Mizumoto,T. Ohsaki,J. Hori,T. Fujii,K. Takamiya,J. Goto,Y. Kiyanagi,K. Kino,M. Furusaka,F. Hiraga,T. Kamiyama 한국물리학회 2011 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.59 No.23
The 4π Ge spectrometer composed of 22-30 Ge detectors was developed, and it has been used to study neutron capture reactions with a neutron time-of-flight (TOF) technique. Since the distances between the sample position and the Ge detectors are variable, it can be used as a multifunctional spectrometer. At first, it was used as a sum energy spectrometer, and resonances in ^(nat)Ag were identified as those of ^(1070Ag or ^(109)Ag. In second, it was used to deduce γ-ray intensity distribution for each resonance, and the results for ^(238)U were presented. In third, it was used to measure neutron capture cross sections of minor actinides and fission products at the electron linear accerelator facility of KURRI and at the accurate neutron-nucleus reaction measurement instrument (ANNRI) in MLF of J-PARC The preliminary results are discussed mainly based on measurements of neutron capture cross section for ^(241)Am, together with related techniques.