Myristoylated TMEM39AS41, a cell‑permeable peptide, causes lung cancer cell death

Sungjin Park,Minhee Kim,Youngeun Hong,Hyunji Lee,Quangdon Tran,Chaeyeong Kim,So Hee Kwon,Jisoo Park,Jongsun Park,Seon‑Hwan Kim 한국독성학회 2020 Toxicological Research Vol.36 No.2

Lung cancer is the most common cause of cancer-associated death worldwide. Most patients with non-small cell lung cancer die within several years of the initial diagnosis, and new therapies are desperately needed. Transmembrane protein (TMEM) 39AS41, a synthetic peptide, was generated from the protein kinase B substrate motif 34GLRNRNGSAIGLPVP48 found in the human TMEM39A protein. Myristic acid was conjugated to the N-terminus of the peptide to confer cell permeability. In this study, we found that in vitro TMEM39AS41 peptide led to cell death via inhibition of inflammation/autophagy pathways in KRAS-mutated cell and tissues. In addition, TMEM39A, at a dose of 30 mg/kg, significantly suppressed tumor growth in KRASLA1 non-small cell lung cancer mice. These results suggest that the TMEM39AS41 peptide could have therapeutic potential for lung cancer.

GOLGA2 loss causes fibrosis with autophagy in the mouse lung and liver

Park, Sungjin,Kim, Sanghwa,Kim, Min Jung,Hong, Youngeun,Lee, Ah Young,Lee, Hyunji,Tran, Quangdon,Kim, Minhee,Cho, Hyeonjeong,Park, Jisoo,Kim, Kwang Pyo,Park, Jongsun,Cho, Myung-Haing Elsevier 2018 Biochemical and biophysical research communication Vol.495 No.1

<P><B>Abstract</B></P> <P>Autophagy is a biological recycling process via the self-digestion of organelles, proteins, and lipids for energy-consuming differentiation and homeostasis. The Golgi serves as a donor of the double-membraned phagophore for autophagosome assembly. In addition, recent studies have demonstrated that pulmonary and hepatic fibrosis is accompanied by autophagy. However, the relationships among Golgi function, autophagy, and fibrosis are unclear. Here, we show that the deletion of <I>GOLGA2</I>, encoding a cis-Golgi protein, induces autophagy with Golgi disruption. The induction of autophagy leads to fibrosis along with the reduction of subcellular lipid storage (lipid droplets and lamellar bodies) by autophagy in the lung and liver. <I>GOLGA2</I> knockout mice clearly demonstrated fibrosis features such as autophagy-activated cells, densely packed hepatocytes, increase of alveolar macrophages, and decrease of alveolar surfactant lipids (dipalmitoylphosphatidylcholine). Therefore, we confirmed the associations among Golgi function, fibrosis, and autophagy. Moreover, <I>GOLGA2</I> knockout mice may be a potentially valuable animal model for studying autophagy-induced fibrosis.</P> <P><B>Highlights</B></P> <P> <UL> <LI> GOLGA2/GM130 loss induces autophagy with Golgi disruption in liver cells and transgenic mice. </LI> <LI> GOLGA2/GM130 loss leads to degradation of lipid structures (LBs and LDs) by autophagy. </LI> <LI> GOLGA2/GM130 loss causes liver and lung fibrosis. </LI> </UL> </P>

Probing Cell-Surface Carbohydrate Binding Proteins with Dual-Modal Glycan-Conjugated Nanoparticles

Park, Sungjin,Kim, Gun-Hee,Park, Seong-Hyun,Pai, Jaeyoung,Rathwell, Dominea,Park, Jin-Yeon,Kang, Young-Sun,Shin, Injae American Chemical Society 2015 JOURNAL OF THE AMERICAN CHEMICAL SOCIETY - Vol.137 No.18

<P>Dual-modal fluorescent magnetic glyconanoparticles have been prepared and shown to be powerful in probing lectins displayed on pathogenic and mammalian cell surfaces. Blood group H1- and Le<SUP>b</SUP>-conjugated nanoparticles were found to bind to BabA displaying <I>Helicobacter pylori</I>, and Le<SUP>a</SUP>- and Le<SUP>b</SUP>-modified nanoparticles are both recognized by and internalized into DC-SIGN and SIGN-R1 expressing mammalian cells via lectin-mediated endocytosis. In addition, glyconanoparticles block adhesion of <I>H. pylori</I> to mammalian cells, suggesting that they can serve as inhibitors of infection of host cells by this pathogen. It has been also shown that owing to their magnetic properties, glyconanoparticles are useful tools to enrich lectin expressing cells. The combined results indicate that dual-modal glyconanoparticles are biocompatible and that they can be employed in lectin-associated biological studies and biomedical applications.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/2015/jacsat.2015.137.issue-18/jacs.5b00592/production/images/medium/ja-2015-00592c_0010.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ja5b00592'>ACS Electronic Supporting Info</A></P>

Effect of the Thermal Conductivity on Resistive Switching in GeTe and Ge2Sb2Te5 Nanowires.

Park, Sungjin,Park, Dambi,Jeong, Kwangsik,Kim, Taeok,Park, SeungJong,Ahn, Min,Yang, Won Jun,Han, Jeong Hwa,Jeong, Hong Sik,Jeon, Seong Gi,Song, Jae Yong,Cho, Mann-Ho American Chemical Society 2015 ACS APPLIED MATERIALS & INTERFACES Vol.7 No.39

<P>The thermal conduction characteristics of GeTe and Ge2Sb2Te5(GST) nanowires were investigated using an optical method to determine the local temperature by Raman spectroscopy. Since the localization of surface charge in a single-crystalline nanostructure can enhance charge-phonon scattering, the thermal conductivity value (관) of single crystalline GeTe and GST nanowires was decreased significantly to 1.44 Wm(-1) K(-1) for GeTe and 1.13 Wm(-1) K(-1) for GST, compared to reported values for polycrystalline structures. The SET-to-RESET state in single-crystalline GeTe and GST nanowires are characteristic of a memory device. Unlike previous reports using GeTe and GST nanowires, the SET-to-RESET characteristics showed a bipolar switching shape and no unipolar switching. In addition, after multiple cycles of operation, a significant change in morphology and composition was observed without any structural phase transition, indicating that atoms migrate toward the cathode or anode, depending on their electronegativities. This change caused by a field effect indicates that the structural phase transition does not occur in the case of GeTe and GST nanowires with a significantly lowered thermal conductivity and stable crystalline structure. Finally, the formation of voids and hillocks as the result of the electromigration critically degrades device reliability.</P>

Multi-functional Capsule Endoscope for Gastro-intestinal Tract

Sukho Park,Jongoh Park,Hyunjun Park,Sungjin Park,Changyeol Jee,Sangkyu Park,Byungkyu Kim 제어로봇시스템학회 2006 제어로봇시스템학회 국제학술대회 논문집 Vol.2006 No.10

Chemically Modified Graphenes

Sungjin Park 한국진공학회 2011 한국진공학회 학술발표회초록집 Vol.40 No.0

FSM에 의한 다층 원통쉘의 동적 특성에 관한 연구

Park, Sungjin 한국재난정보학회 2015 한국재난정보학회 논문집 Vol.11 No.4

본 연구는 양단이 단순지지된 조건을 갖는 다층 원통쉘을 해석하는 방법을 제시하였고, 3차원 응력 특성을 규명한 것이다. 지배방정식은 편미분방정식을 상미분방정식으로 변환을 가정한 유한요소 개념을 이용하여 유한대판법 해석법을 이용하여 수치해서하였다. 특히 단순지지 조건을 갖는 3차원 다층원통쉘에 대해서는 시행함수로서 삼각함수로 구성되는 보의 고유함수로 구성되는 경우에 대해 해석하였다. 층 재료는 강재 또는 콘크리트로하고 층두께, 원통길이 등 파라메터를 다양하게 변화시켜 다층원통쉘에 미치는 영향을 검토한다. This study was presented how to interpret a laminated cylindrical shell having both ends supported condition are simple, and by identifying the three-dimensional stress characteristics. The governing equations were using the concept of finite element assuming the conversion into ordinary differential equations and partial differential equations by numerical analysis using the finite strip method. In particular, a function performed for the three-dimensional laminated cylindrical shell having a simple support condition were analyzed for the case composed of a specific function of the beam consisting of a trigonometric function. Layer material and layer thickness of a steel or concrete, the cylindrical length and the like by varying the parameters variously examine the effects of multi-layer cylindrical shell.

Exposure to pesticides and the prevalence of diabetes in a rural population in Korea

Park, Sungjin,Kim, Sung-Kyung,Kim, Jae-Yeop,Lee, Kyungsuk,Choi, Jung Ran,Chang, Sei-Jin,Chung, Choon Hee,Park, Kyu-Sang,Oh, Sung-Soo,Koh, Sang-Baek Elsevier 2019 Neurotoxicology Vol.70 No.-

<P><B>Abstract</B></P> <P><B>Background</B></P> <P>Among the adverse health effects of exposure to pesticides, an association with diabetes has been reported. However, there is a lack of epidemiologic studies on the health effects of exposure to pesticides, particularly investigating the association between occupational pesticide exposure and diabetes prevalence.</P> <P><B>Purpose</B></P> <P>The present study examined the association between pesticide exposure and prevalence of diabetes in a rural population in Korea.</P> <P><B>Methods</B></P> <P>This cross-sectional study used data from the Korea Farmers Cohort study, and included 2559 participants in the baseline survey between November 2005 and January 2008. We performed a clinical examination including blood sampling and assessed data on diabetes diagnosis, demographics, and pesticide exposure. Logistic regression was performed to evaluate the association between pesticide exposure and diabetes prevalence, adjusting for age, sex, monthly income, and marital status. In addition, a stratified analysis by body mass index (BMI) was conducted, with two categories: normal weight (<25 kg/m<SUP>2</SUP>) and overweight or obese (≥25 kg/m<SUP>2</SUP>).</P> <P><B>Results</B></P> <P>At baseline, the prevalence of diabetes was 9.30%. Pesticide exposure was associated with the risk of diabetes after adjustment for covariates. In the analysis stratified by BMI, all the variables related to pesticide exposure were associated with prevalence of diabetes in the overweight or obese group, whereas no significant association was found in the normal weight group.</P> <P><B>Conclusion</B></P> <P>Exposure to pesticides was associated with diabetes, and this association was stronger in overweight or obese individuals than in normal weight individuals. Further longitudinal studies that consider information on BMI are necessary.</P> <P><B>Highlights</B></P> <P> <UL> <LI> We report an association between pesticide exposure and diabetes in Korean adults. </LI> <LI> The association was stronger in overweight/obese than in normal weight people. </LI> <LI> Pesticide use may contribute to high rates of diabetes. </LI> </UL> </P>

4He adsorption on a single C40 molecule: Path integral Monte Carlo study

Park, Sungjin,Kwon, Yongkyung 한국물리학회 2018 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol. No.

Multiple pathways are involved in palmitic acid-induced toxicity

Park, Eun-Jung,Lee, Ah Young,Park, Sungjin,Kim, Jae-Ho,Cho, Myung-Haing Elsevier 2014 Food and chemical toxicology Vol.67 No.-

<P>In this study, we identified the toxic mechanism following the accumulation of palmitic acid (PA), a saturated fatty acid, in human Chang liver cells. After PA exposure for 24 h, the mitochondria and the endoplasmic reticulum (ER) became dilated, and lipid droplets and organelles were observed within autophagosomes. Cell viability decreased with an ATP reduction and the G2/M phase arrest. The expression of SOD-2, but not of SOD-1, markedly increased after PA exposure, which also elevated the number of cells generating ROS. PA enhanced the levels of proteins related to apoptosis, necroptosis, autophagy, and ER stress. Moreover, the inhibition of caspases, p53, necroptosis, or ER stress substantially rescued PA-induced cytotoxicity and, similarly, the inhibition of caspases and ER stress counteracted PA-induced changes in the cell cycle. Conversely, the inhibition of necroptosis and p53 signaling accelerated the changes in the cell cycle triggered by PA exposure. Blocking autophagy exacerbated PA-induced cytotoxicity and alterations in the cell cycle and caused disappearance of cellular components. These results suggest that PA induces apoptosis accompanied by autophagy through mitochondrial dysfunction and ER stress, which are triggered by oxidative stress in Chang liver cells and that blocking autophagy accelerates cell damage following PA exposure.</P>