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GOLGA2 loss causes fibrosis with autophagy in the mouse lung and liver
Park, Sungjin,Kim, Sanghwa,Kim, Min Jung,Hong, Youngeun,Lee, Ah Young,Lee, Hyunji,Tran, Quangdon,Kim, Minhee,Cho, Hyeonjeong,Park, Jisoo,Kim, Kwang Pyo,Park, Jongsun,Cho, Myung-Haing Elsevier 2018 Biochemical and biophysical research communication Vol.495 No.1
<P><B>Abstract</B></P> <P>Autophagy is a biological recycling process via the self-digestion of organelles, proteins, and lipids for energy-consuming differentiation and homeostasis. The Golgi serves as a donor of the double-membraned phagophore for autophagosome assembly. In addition, recent studies have demonstrated that pulmonary and hepatic fibrosis is accompanied by autophagy. However, the relationships among Golgi function, autophagy, and fibrosis are unclear. Here, we show that the deletion of <I>GOLGA2</I>, encoding a cis-Golgi protein, induces autophagy with Golgi disruption. The induction of autophagy leads to fibrosis along with the reduction of subcellular lipid storage (lipid droplets and lamellar bodies) by autophagy in the lung and liver. <I>GOLGA2</I> knockout mice clearly demonstrated fibrosis features such as autophagy-activated cells, densely packed hepatocytes, increase of alveolar macrophages, and decrease of alveolar surfactant lipids (dipalmitoylphosphatidylcholine). Therefore, we confirmed the associations among Golgi function, fibrosis, and autophagy. Moreover, <I>GOLGA2</I> knockout mice may be a potentially valuable animal model for studying autophagy-induced fibrosis.</P> <P><B>Highlights</B></P> <P> <UL> <LI> GOLGA2/GM130 loss induces autophagy with Golgi disruption in liver cells and transgenic mice. </LI> <LI> GOLGA2/GM130 loss leads to degradation of lipid structures (LBs and LDs) by autophagy. </LI> <LI> GOLGA2/GM130 loss causes liver and lung fibrosis. </LI> </UL> </P>
Myristoylated TMEM39AS41, a cell‑permeable peptide, causes lung cancer cell death
Sungjin Park,Minhee Kim,Youngeun Hong,Hyunji Lee,Quangdon Tran,Chaeyeong Kim,So Hee Kwon,Jisoo Park,Jongsun Park,Seon‑Hwan Kim 한국독성학회 2020 Toxicological Research Vol.36 No.2
Lung cancer is the most common cause of cancer-associated death worldwide. Most patients with non-small cell lung cancer die within several years of the initial diagnosis, and new therapies are desperately needed. Transmembrane protein (TMEM) 39AS41, a synthetic peptide, was generated from the protein kinase B substrate motif 34GLRNRNGSAIGLPVP48 found in the human TMEM39A protein. Myristic acid was conjugated to the N-terminus of the peptide to confer cell permeability. In this study, we found that in vitro TMEM39AS41 peptide led to cell death via inhibition of inflammation/autophagy pathways in KRAS-mutated cell and tissues. In addition, TMEM39A, at a dose of 30 mg/kg, significantly suppressed tumor growth in KRASLA1 non-small cell lung cancer mice. These results suggest that the TMEM39AS41 peptide could have therapeutic potential for lung cancer.
Probing Cell-Surface Carbohydrate Binding Proteins with Dual-Modal Glycan-Conjugated Nanoparticles
Park, Sungjin,Kim, Gun-Hee,Park, Seong-Hyun,Pai, Jaeyoung,Rathwell, Dominea,Park, Jin-Yeon,Kang, Young-Sun,Shin, Injae American Chemical Society 2015 JOURNAL OF THE AMERICAN CHEMICAL SOCIETY - Vol.137 No.18
<P>Dual-modal fluorescent magnetic glyconanoparticles have been prepared and shown to be powerful in probing lectins displayed on pathogenic and mammalian cell surfaces. Blood group H1- and Le<SUP>b</SUP>-conjugated nanoparticles were found to bind to BabA displaying <I>Helicobacter pylori</I>, and Le<SUP>a</SUP>- and Le<SUP>b</SUP>-modified nanoparticles are both recognized by and internalized into DC-SIGN and SIGN-R1 expressing mammalian cells via lectin-mediated endocytosis. In addition, glyconanoparticles block adhesion of <I>H. pylori</I> to mammalian cells, suggesting that they can serve as inhibitors of infection of host cells by this pathogen. It has been also shown that owing to their magnetic properties, glyconanoparticles are useful tools to enrich lectin expressing cells. The combined results indicate that dual-modal glyconanoparticles are biocompatible and that they can be employed in lectin-associated biological studies and biomedical applications.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/2015/jacsat.2015.137.issue-18/jacs.5b00592/production/images/medium/ja-2015-00592c_0010.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ja5b00592'>ACS Electronic Supporting Info</A></P>
Effect of the Thermal Conductivity on Resistive Switching in GeTe and Ge2Sb2Te5 Nanowires.
Park, Sungjin,Park, Dambi,Jeong, Kwangsik,Kim, Taeok,Park, SeungJong,Ahn, Min,Yang, Won Jun,Han, Jeong Hwa,Jeong, Hong Sik,Jeon, Seong Gi,Song, Jae Yong,Cho, Mann-Ho American Chemical Society 2015 ACS APPLIED MATERIALS & INTERFACES Vol.7 No.39
<P>The thermal conduction characteristics of GeTe and Ge2Sb2Te5(GST) nanowires were investigated using an optical method to determine the local temperature by Raman spectroscopy. Since the localization of surface charge in a single-crystalline nanostructure can enhance charge-phonon scattering, the thermal conductivity value (관) of single crystalline GeTe and GST nanowires was decreased significantly to 1.44 Wm(-1) K(-1) for GeTe and 1.13 Wm(-1) K(-1) for GST, compared to reported values for polycrystalline structures. The SET-to-RESET state in single-crystalline GeTe and GST nanowires are characteristic of a memory device. Unlike previous reports using GeTe and GST nanowires, the SET-to-RESET characteristics showed a bipolar switching shape and no unipolar switching. In addition, after multiple cycles of operation, a significant change in morphology and composition was observed without any structural phase transition, indicating that atoms migrate toward the cathode or anode, depending on their electronegativities. This change caused by a field effect indicates that the structural phase transition does not occur in the case of GeTe and GST nanowires with a significantly lowered thermal conductivity and stable crystalline structure. Finally, the formation of voids and hillocks as the result of the electromigration critically degrades device reliability.</P>
Park, Sungjin 한국재난정보학회 2015 한국재난정보학회 논문집 Vol.11 No.4
본 연구는 양단이 단순지지된 조건을 갖는 다층 원통쉘을 해석하는 방법을 제시하였고, 3차원 응력 특성을 규명한 것이다. 지배방정식은 편미분방정식을 상미분방정식으로 변환을 가정한 유한요소 개념을 이용하여 유한대판법 해석법을 이용하여 수치해서하였다. 특히 단순지지 조건을 갖는 3차원 다층원통쉘에 대해서는 시행함수로서 삼각함수로 구성되는 보의 고유함수로 구성되는 경우에 대해 해석하였다. 층 재료는 강재 또는 콘크리트로하고 층두께, 원통길이 등 파라메터를 다양하게 변화시켜 다층원통쉘에 미치는 영향을 검토한다. This study was presented how to interpret a laminated cylindrical shell having both ends supported condition are simple, and by identifying the three-dimensional stress characteristics. The governing equations were using the concept of finite element assuming the conversion into ordinary differential equations and partial differential equations by numerical analysis using the finite strip method. In particular, a function performed for the three-dimensional laminated cylindrical shell having a simple support condition were analyzed for the case composed of a specific function of the beam consisting of a trigonometric function. Layer material and layer thickness of a steel or concrete, the cylindrical length and the like by varying the parameters variously examine the effects of multi-layer cylindrical shell.
Park, Sul Ki,Choi, Kwangrok,Lee, Si-Hwa,Oh, Il-Kwon,Park, Sungjin,Park, Ho Seok Elsevier 2017 Carbon Vol.116 No.-
<P>The hierarchically architectured graphene-based materials are considered as a promising active or supporting material for the lithium ion batteries (LIBs). However, it faces critical challenges of the limited stored capacity, the mechanical brittleness, and the contact resistance. Herein, we demonstrate the unique hierarchical structures, where carbon nanotubes (CNTs) are branched onto the modified surfaces of three-dimensional (3D), steam-activated reduced graphene oxide (sRG-O) frameworks, prepared by self-assembly, steam activation, and microwave methods. The surface sites of 3D sRG-O are critical for controlling crystalline structure and deposition density of Fe3O4 nanoparticles through a microwave induced synthesis, as well as for providing a large surface area and conducting pathway. Simultaneously, the bamboo-like CNT branches are grown on the Fe3O4 nanoparticles acting as catalysts to stabilize and conductively connect 3D sRG-O/Fe interparticles for the enhanced rate and cyclic performances of LIB. Such a unique structure consisting of 1D nanostructure branched on the activated surface of 3D macroporous structure with decoration of OD nanoparticles provides high specific capacity of 1757 mAh g(-1) at 50 mA g(-1), good rate capability of 73.31% at 1000 mA g(-1), and gradual increase from 1490 to 2890 mAh g(-1) after 100 cycles. (C) 2017 Elsevier Ltd. All rights reserved.</P>
Park, Sungjin,Moon, SeongRyeol,Lee, Kiyoung,Park, Ie Byung,Lee, Dae Ho,Nam, Seungyoon S. Karger AG 2018 CELLULAR PHYSIOLOGY AND BIOCHEMISTRY Vol.46 No.4
<P><B><I>Background/Aims:</I></B> Diabetic nephropathy (DN), a major diabetic microvascular complication, has a long and growing list of biomarkers, including microRNA biomarkers, which have not been consistent across preclinical and clinical studies. This meta-analysis aims to identify significant blood- and urine-incident microRNAs as diagnostic/prognostic biomarker candidates for DN. <B><I>Methods:</I></B> PubMed, Web of Science, and Cochrane Library were searched from their earliest records through 12th Dec 2016. Relevant publications for the meta-analysis included (1) human participants; (2) microRNAs in blood and urine; (3) DN studies; and (4) English language. Four reviewers, including two physicians, independently and blindly extracted published data regarding microRNA profiles in blood and/or urine from subjects with diabetic nephropathy. A random-effect model was used to pool the data. Statistical associations between diabetic nephropathy and urinary or blood microRNA expression levels were assessed. <B><I>Results:</I></B> Fourteen out of 327 studies (n=2,747 patients) were selected. Blood or urinary microRNA expression data of diabetic nephropathy were pooled for this analysis. The hsa-miR-126 family was significantly (OR: 0.57; 95% CI: 0.44-0.74; p-value < 0.0001) downregulated in blood from patients with diabetic kidney disease, while its urinary level was upregulated (OR: 2931.12; 95% CI: 9.96-862623.21; p-value = 0.0059). The hsa-miR-770 family microRNA were significantly (OR: 10.24; 95% CI: 2.37-44.25; p-value = 0.0018) upregulated in both blood and urine from patients with diabetic nephropathy. <B><I>Conclusions:</I></B> Our meta-analysis suggests that hsa-miR-126 and hsa-miR-770 family microRNA may have important diagnostic and pathogenetic implications for DN, which warrants further systematic clinical studies.</P>