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참깨의 種間雜種育成에 관한 硏究 Ⅰ. 참깨 野生種의 特性과 交雜親和性
Jung Il Lee(李正日),Bong Ho Lee(李奉鎬),Nak Sul Seong(成洛戌),Chui Whan Kang(姜哲煥) 한국육종학회 1990 한국육종학회지 Vol.22 No.4
To utilize wild alien gene sources for multi-resistance to several major diseases of sesame, two wild species, Sesamum alatum (2n=26) and S. radiatum (2n=64) were examined on the agronomical characters and cross affinities with cultivated species. S. radiatum showed perennial habit while S. alatum appeared on annual plant habitate like cultivated species. Both species are branched and late maturing with light grain weight. They were highly resistance to seedling blight, phytophthora blight, fusarium wilt and leaf blight. Their cross affinities with cultivated species were very high with bearing capsules from 8 to 70% in the almost all the crosses. However, those seeds were not germinated mainly due to immaturity. Therefore it may be necessary to rescue fertilized-embryos by in vitro technique.
참깨 開花 登熟에 關한 硏究 Ⅹ. 참깨 有限 新草型의 開花 및 착삭習性
Chul Whan Kang(姜哲煥),Jung Il Lee(李定日),Bong Ho Lee(李奉鎬),Nak Sul Seong(成洛戌),Rae Kyeong Park(朴來敬) 한국육종학회 1991 한국육종학회지 Vol.23 No.4
This experiment was performed to investigate the flowering and capsule setting habit of newly developed determinate mutant in sesame (Sesamum indicum L.). Varieties tested could be classified into new plant type by it’s morphological trait of determinance with capsule shape, capsule setting and branching habit compared to reported 9 different plant types in this series. Flowers were appeared progressively toward the tip of the main stem and also toward the tips of branches except the terminal flower flowering at the 2nd day on each main stem and branchs of DBTB terminal (Determinate, Branches, Tricapsules, Bicarpels Quadriloculi) type. Flowers and capsules were beared on secondary branches in DBTB type which is contrasted with unbearing secondary branches in indeterminate type. Flowering order of DBTB type was the earliest on main stem, intermediate on primary branches and the latest on secondary branches, and flowers were appeared progressively from upper part toward lower part in primary branches. DBTB type showed higher inflorescence due to 12 days shorter flowering period compared to that of indeterminate type therefore this will be idealistic character for the purpose of breeding combining harvester adapting varieties in sesame. Capsule bearing ratio was the highest on main stem, intermediate on primary branches and the lowest on secondary branches in DBTB type. Capsule bearing ratio of DBTB type showed decreasing tendancy day by day after first flowering. DBTB type was considered as ideal plant type for labour saving combining harvesting with higher yield ability due to it’s higher inflorescence, flowering and capsule bearing on even secondary branches which do not develop in indeterminate plant types.
Cho, Jae Whan,Sul, Kyun Il The Korean Fiber Society 2001 Fibers and polymers Vol.2 No.3
Organic-inorganic hybrid composites consisting of poly(vinylidene fluoride) (PVDF) and SiO$_2$ were prepared through a sol-gel process and the crystallization behavior of PVDF in the presence of $SiO_2$ networks was investigated by spectroscopic, thermal and x-ray diffraction measurements. The hybrid composites obtained were relatively transparent, and brittleness increased with increasing content of tetraethoxysilane (TEOS). It was regarded from FT-lR and DSC thermal analyses that at least a certain interaction existed between PVDF molecules and the $SiO_2$ networks. X-ray diffraction measurements showed that all of the hybrid samples had a crystal structure of PVDF ${\gamma}$-phase. Fresh gel prepared from the sol-gel reaction showed a very weak x-ray diffraction peak near 2$\theta$=$21^{\circ}$ due to PVDF crystallization, and Intensity increased grade-ally with time after gelation. The crystallization behavior of PVDF was strongly affected by the amount of $SiO_2$ networks. That is, $SiO_2$ content directly influenced preference and disturbance fur crystallization. In polymer-rich hybrids, $SiO_2$ networks had a favorable effect on the extent of PVDF crystallization. In particular, the maximum portent crystallinity of PVDF occurred at the content of 3.7 wt% $SiO_2$ and was higher than that of pure PVDF. However. beyond about 10 wt% $SiO_2$, the crystallization of PVDF was strongly confined.
Lusta, Konstantin A.,Chung, Il Kyung,Sul, Ill Whan,Park, Hee Sung,Shin, Dong Ill 한국미생물 · 생명공학회 1999 Journal of microbiology and biotechnology Vol.9 No.6
Aspergillus sp. CX-1 strain grown on microcrystalline cellulose resulted in the accumulation of high levels of cellulase and xylanase activities that were higher by two to four folds than those from the conventional commercial producer, Trichoderma reesei QM9414. Aspergillus sp. CX-1 demonstrated greater thermostability and better catalytic characteristics of total cellulase activity (FPase) as compared to T. reesei and Aspergillus niger F-2039.
참깨의 開花 및 登熟에 관한 硏究 Ⅸ. 참깨 有限開花性의 遺傳分析
Bong Ho Lee(李奉鎬),Jung Il Lee(李正日),Nak Sul Seong(成洛戌),Rae Kyeong Park(朴來敬),Sung Il Kim(金成日),Chul Whan Kang(姜哲煥) 한국육종학회 1990 한국육종학회지 Vol.22 No.1
Breeding of determinate-flowering type in sesame is being attempted in Crop Experiment Station. In an attempt to make a gene analysis of the determinate-flowering characteristics, two set of F₁, F₂, BC₁F₁, BC₂F₁ which made using Ahnsan and Hanseom as an indeterminate parent and SIGH8705-2 as a determinate parent were applied in the experiment in 1989. Among the characteristics of determinate parent, number of capsules per plant were lesser than those of the indeterminate varieties which is important characteristics to be improved in determinate-flowering type. Inheritance of determinate-flowering characteristics is recognized to be controlled by the single recessive gene, from the segregation ratio of indeterminate and determinate as 3:1 in F₂ population of indeterminate and determinate cross, and 1:1 in test cross of BC₂F₁ population.
참깨의 種間雜種 育成에 관한 硏究 Ⅱ. S. indicum × S. radiatum間의 種間雜種 育成
Bong Ho Lee(李奉鎬),Jung Il Lee(李正日),Nak Sul Seong(成洛戌),Chul Whan Kang(姜哲煥),Chung Hun Park(朴忠獻) 한국육종학회 1991 한국육종학회지 Vol.23 No.1
To breed a multi-resistant midparent for several major diseases in sesame, interspecific hybridization between S. indicum (2n=26) and S. radiatum(2n=64) was attempted by the premature embryo culture method in 1988 at the green house and culture room of the Crop Experiment Station. Before the hybrid embryo culture attempted, pre-experiments were performed with selfed seed embryo in each S. indicum and S. radiatum to confirm the optimum culture time, seed part and media of which applied in premature embryo culture of the interspecific hybridization. With the application of the pre-experiment results, premature hybrid embryos were cultured on MS medium with 16-20 days old embryo. Six plants of which 2.52% of the cultured embryo were induced from the premature hybrid embryo culture of S. indicum×S. radiatum. The six plants showed intermediate of the parents in plant characteristics with 2n=45 somatic chromosomes and semi-sterility. The progenies derived from the interspecific cross is being tested of their characteristics and disease resistance in field condition.
효모의 미토콘드리아 형질전환을 통한 인위적인 operon 형식의 유전자 발현 규명
김경민,설일환,Kim Kyung-Min,Sul Il-Whan 한국생명과학회 2006 생명과학회지 Vol.16 No.3
본 실험에서는 식물의 유용유전자를 개발하여 그 발현양상을 확인하기 위하여 효모를 이용하면 그 발현양상을 비교적 빠르게 확인할 수 있는 미토콘드리아 형질전환 방법을 규명하였다. 또한 미토콘드리아(mt)에 관련된 유전자를 TPI promoter를 가진 plasmid에 재조합한 후 효모에 형질전환하여 mt에서 그 유전자의 특성이 발현 되는 것을 확인하였다. 따라서 본 연구의 결과로 mt에 관련된 유전자를 식물의 조직에 형질전환 하여 1개 이상의 유전자가 식물의 mt에 삽입되어 그 유전자의 특성이 발현되는데 이용되어 질수 있을 것이라 생각된다. Yeast mitochondrial transformation has been confirmed by cell death and CFP expression (CDF: cell death factor gene). Expression vector containing CDF and CFP driven by one TPI (Triose-phosphate isomerase) promoter (called artificial operon type) was bombarded to Yeast. Interestingly, yeast cells were progressively deformed into unusual shapes and lysed inner cytoplasm resulting in ell death after all after bombarding with expression vector (CDC and GFP). Since there is no report about more than one gene expression simultaneously in a single mitochondria, this report is very important to novel type of eukaryotic gene expression. Successful yeast cell transformation in this report implies possible eukaryotic mitochodrial transformation including plants and animals and moreover two or more gene expression which can be excellent applicable protocols to pharmaceutical field including antibody production.