http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Son Singh (검색결과 20 건)
- 무료
- 기관 내 무료
- 유료
-
Son Young-Bum,Jeong Yeon Ik,Jeong Yeon Woo,Olsson Per Olof,Hossein Mohammad Shamim,Cai Lian,Kim Sun,Choi Eun Ji,Sakaguchi Kenichiro,Tinson Alex,Singh Kuhad Kuldip,Rajesh Singh,Noura Al Shamsi,Hwang Wo 아세아·태평양축산학회 2022 Animal Bioscience Vol.35 No.2
Objective: The present study evaluated the efficiency of embryo development and pregnancy of somatic cell nuclear transfer (SCNT) embryos using different source-matured oocytes in Camelus dromedarius. Methods: Camelus dromedarius embryos were produced by SCNT using in vivo- and in vitro- matured oocytes. In vitro embryo developmental capacity of reconstructed embryos was evaluated. To confirm the efficiency of pregnancy and live birth rates, a total of 72 blastocysts using in vitro- matured oocytes transferred into 45 surrogates and 95 blastocysts using in vivo- matured oocytes were transferred into 62 surrogates by transvaginal method. Results: The collected oocytes derived from ovum pick up showed higher maturation potential into metaphase II oocytes than oocytes from the slaughterhouse. The competence of cleavage, and blastocyst were also significantly higher in in vivo- matured oocytes than in vitro- matured oocytes. After embryo transfer, 11 pregnant and 10 live births were confirmed in in vivo- matured oocytes group, and 2 pregnant and 1 live birth were confirmed in in vitro- matured oocytes group. Furthermore, blastocysts produced by in vivo-matured oocytes resulted in significantly higher early pregnancy and live birth rates than in vitromatured oocytes. Conclusion: In this study, SCNT embryos using in vivo- and in vitro-matured camel oocytes were successfully developed, and pregnancy was established in recipient camels. We also confirmed that in vivo-matured oocytes improved the development of embryos and the pregnancy capacity using the blastocyst embryo transfer method. Objective: The present study evaluated the efficiency of embryo development and pregnancy of somatic cell nuclear transfer (SCNT) embryos using different source-matured oocytes in Camelus dromedarius.Methods: Camelus dromedarius embryos were produced by SCNT using in vivo- and in vitro- matured oocytes. In vitro embryo developmental capacity of reconstructed embryos was evaluated. To confirm the efficiency of pregnancy and live birth rates, a total of 72 blastocysts using in vitro- matured oocytes transferred into 45 surrogates and 95 blastocysts using in vivo- matured oocytes were transferred into 62 surrogates by transvaginal method.Results: The collected oocytes derived from ovum pick up showed higher maturation potential into metaphase II oocytes than oocytes from the slaughterhouse. The competence of cleavage, and blastocyst were also significantly higher in in vivo- matured oocytes than in vitro- matured oocytes. After embryo transfer, 11 pregnant and 10 live births were confirmed in in vivo- matured oocytes group, and 2 pregnant and 1 live birth were confirmed in in vitro- matured oocytes group. Furthermore, blastocysts produced by in vivo-matured oocytes resulted in significantly higher early pregnancy and live birth rates than in vitromatured oocytes.Conclusion: In this study, SCNT embryos using in vivo- and in vitro-matured camel oocytes were successfully developed, and pregnancy was established in recipient camels. We also confirmed that in vivo-matured oocytes improved the development of embryos and the pregnancy capacity using the blastocyst embryo transfer method.
-
Singh, Amit,Tare, Meghana,Kango-Singh, Madhuri,Son, Won-Seok,Cho, Kyung-Ok,Choi, Kwang-wook Elsevier 2011 Developmental biology Vol.359 No.2
<P><B>Abstract</B></P><P>Patterning in multi-cellular organisms involves progressive restriction of cell fates by generation of boundaries to divide an organ primordium into smaller fields. We have employed the <I>Drosophila</I> eye model to understand the genetic circuitry responsible for defining the boundary between the eye and the head cuticle on the ventral margin. The default state of the early eye is ventral and depends on the function of <I>Lobe (L)</I> and the Notch ligand <I>Serrate (Ser)</I>. We identified <I>homothorax (hth)</I> as a strong enhancer of the <I>L</I> mutant phenotype of loss of ventral eye. Hth is a MEIS class gene with a highly conserved Meis-Hth (MH) domain and a homeodomain (HD). Hth is known to bind Extradenticle (Exd) via its MH domain for its nuclear translocation. Loss-of-function of <I>hth</I>, a negative regulator of eye, results in ectopic ventral eye enlargements. This phenotype is complementary to the <I>L</I> mutant phenotype of loss-of-ventral eye. However, if <I>L</I> and <I>hth</I> interact during ventral eye development remains unknown. Here we show that (i) <I>L</I> acts antagonistically to <I>hth</I>, (ii) Hth is upregulated in the <I>L</I> mutant background, and (iii) MH domain of Hth is required for its genetic interaction with L, while its homeodomain is not, (iv) in <I>L</I> mutant background ventral eye suppression function of Hth involves novel MH domain-dependent factor(s), and (v) nuclear localization of Exd is not sufficient to mediate the Hth function in the <I>L</I> mutant background. Further, Exd is not a critical rate-limiting factor for the Hth function. Thus, optimum levels of L and Hth are required to define the boundary between the developing eye and head cuticle on the ventral margin.</P> <P><B>Highlights</B></P><P>► We study the genetic mechanism involved in generation of head versus eye boundary during organogenesis in flies. ► <I>homothorax (hth)</I> acts antagonistically to the <I>Lobe</I> (<I>L</I>, PRAS40 homolog) in the ventral eye. ► L interacts with alternative spliced variant of Hth that encodes Meis-Hth (MH) domain. ► Optimum level of <I>L</I> and <I>hth</I> define boundary between eye and the head cuticle on ventral margin. ► Extradenticle (Exd) is not a critical rate limiting factor in L and Hth interaction.</P>
-
-
Son, S. Y.,Kim, N. K.,Lee, S.,Singh, D.,Kim, G. R.,Lee, J. S.,Yang, H. s.,Yeo, J.,Lee, S.,Lee, C. H. Springer Science + Business Media 2016 Plant cell reports Vol.35 No.9
<P>A multi-parallel approach gauging the mass spectrometry-based metabolite fingerprinting coupled with bioactivity and pathway evaluations could serve as an efficacious tool for inferring plant taxonomic orders. Thirty-four species from three plant families, namely Cornaceae (7), Fabaceae (9), and Rosaceae (18) were subjected to metabolite profiling using gas chromatography-time-of-flight-mass spectrometry (GC-TOF-MS) and ultrahigh performance liquid chromatography-linear trap quadrupole-ion trap-mass spectrometry (UHPLC-LTQ-IT-MS/MS), followed by multivariate analyses to determine the metabolites characteristic of these families. The partial least squares discriminant analysis (PLS-DA) revealed the distinct clustering pattern of metabolites for each family. The pathway analysis further highlighted the relatively higher proportions of flavonols and ellagitannins in the Cornaceae family than in the other two families. Higher levels of phenolic acids and flavan-3-ols were observed among species from the Rosaceae family, while amino acids, flavones, and isoflavones were more abundant among the Fabaceae family members. The antioxidant activities of plant extracts were measured using ABTS, DPPH, and FRAP assays, and indicated that extracts from the Rosaceae family had the highest activity, followed by those from Cornaceae and Fabaceae. The correlation map analysis positively links the proportional concentration of metabolites with their relative antioxidant activities, particularly in Cornaceae and Rosaceae. This work highlights the pre-eminence of the multi-parallel approach involving metabolite profiling and bioactivity evaluations coupled with metabolic pathways as an efficient methodology for the evaluation of plant phylogenies.</P>
-
Toxic effects of mercuric sulfide on immune organs in mice.
Son, Hee-Young,Lee, Soyoung,Park, Seung-Bin,Kim, Mi-Sun,Choi, Eun-Ju,Singh, Thoudam S K,Bae, Yunju,Kwack, Seung Jun,Kang, Tae Seok,Shin, Hong-In,Baek, Moon-Chang,Kim, Sang-Hyun Marcel Dekker 2010 IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY Vol.32 No.2
<P>Mercuric sulfide (HgS) is a major component of cinnabar, which has been used as a sedative drug in China for more than 2000 years. Because its toxicological effects are still unclear, we attempted to verify the toxic effects of HgS, focused on liver and immune organs such as the spleen and thymus. Male ICR mice were administered HgS (0.02, 0.2, 2.0 g/kg/day) by gavage for 4 weeks. During the administration period, HgS-treated mice did not reveal overt signs of clinical toxicity. HgS had no significant effect on body weight, food consumption, water consumption, and organ weights. In spite of its known insolubility, HgS was absorbed by the gastrointestinal tract and accumulated in the liver, spleen and thymus in a dose-dependent manner. In the biochemical and histological examination, HgS did not cause hepatotoxicity. However, HgS significantly increased both CD8(+) T lymphocytes and CD4(+)CD8(+) lymphocyte populations in the spleen without changing in the thymus. In the histological evaluation, HgS induced enlargement with marked hyperplasia and increase of lymphoid follicles in the spleen. In addition, HgS induced the gene expression of pro-inflammatory cytokines in the spleen and thymus. Our results suggest that insoluble HgS was absorbed by the gastrointestinal tract, accumulated in the spleen and thymus, and thus could affect immune systems.</P>
-
Haryanto,Singh, D.,Han, S.S.,Son, J.H.,Kim, S.C. Elsevier 2015 Materials Science and Engineering C Vol.46 No.-
The cross-linked poly(ethylene glycol) dicarboxylate (PEGDC)/poly(ethylene oxide) (PEO) and poly(ethylene glycol) dimethacrylate (PEGDMA)/(PEO) hydrogels were developed for possible biomedical applications such as an anti-adhesion barrier. Various contents of PEGDC/PEO film were irradiated using an electron beam with various beam intensities in order to obtain various degrees of crosslinked hydrogels. The optimum dose (300kGy) and total crosslinker content of 10% were used to prepare crosslinked hydrogel films with three different compositions (10% PEGDC, 10% PEGDMA, 5% PEGDC-5% PEGDMA). Among them, 10% PEGDC hydrogel film exhibited the highest elongation at break (69.33+/-6.87%) with high mechanical strength. 10% PEGDC hydrogel film showed the lowest hemolysis activity (6.03+/-0.01%) and the highest tissue adherence (75.67+/-1.15cN). The result also indicated that the carboxyl groups in PEGDC affect the tissue adherence of hydrogel films via H-bonding interactions. In animal studies, 10% PEGDC anti-adhesion hydrogel film degraded within 3weeks and demonstrated better anti-adhesive effect compared to Guardix-SG®.
-
-
Young-Bum Son,Mohammad Shamim Hossein,Yeon Ik Jeong,Mina Kang,Huijeong Kim,Yura Bae,Kung Ik Hwang,Alex Tinson,Singh Rajesh,Al Shamsi Noura,Woo Suk Hwang The Korean Society of Animal Reproduction and Biot 2024 한국동물생명공학회지 Vol.39 No.1
Background: Somatic cell nuclear transfer (SCNT) is a prominent technology that can preserve superior genetic traits of animals and expand the population in a short time. Hematological characters and endocrine profiles are important elements that demonstrate the stability of the physiological state of cloned animals. To date, several studies regarding cloned camels with superior genes have been conducted. However, detailed hemato-physiological assessments to prove that cloned camels are physiologically normal are limited. In this study, We evaluated the hemato-physiological characteristics of cloned male and female dromedary camels (Camelus dromedaries). Methods: Therefore, we analyzed variations in hematological characteristics and endocrine profiles between cloned and non-cloned age-matched male and female dromedary camels (Camelus dromedaries). Two groups each of male and female cloned and non-cloned camels were monitored to investigate the differences in hemato-physiological characteristics. Results: All the animals were evaluated by performing complete blood count (CBC), serum chemistry, and endocrinological tests. We found no significant difference between the cloned and non-cloned camels. Furthermore, the blood chemistry and endocrine profiles in male and female camels before maturity were similar. Conclusions: These results suggest that cloned and non-cloned camels have similar hematological characteristics and endocrine parameters.
-
<i>Rhodanobacter koreensis</i> sp. nov., a bacterium isolated from tomato rhizosphere
Won, KyungHwa,Singh, Hina,Ngo, Hien T. T.,Son, HeungMin,Kook, MooChang,Kim, Ki–,Young,Yi, Tae–,Hoo International Union of Microbiological Societies 2015 International journal of systematic and evolutiona Vol.65 No.4
<P>A Gram-stain-negative, aerobic, rod-shaped and motile bacterium, designated THG-DD7<SUP>T</SUP>, was isolated from tomato plant rhizosphere soil. Strain THG-DD7<SUP>T</SUP> grew optimally at 25–30 °C, at pH 7.0–7.5 and in the presence of 0.5 % (w/v) NaCl. According to the results of 16S rRNA gene sequence comparisons, strain THG-DD7<SUP>T</SUP> was most closely related to <I>Rhodanobacter umsongensis</I> GR24-2<SUP>T</SUP> (98.2 %), <I>Rhodanobacter panaciterrae</I> LnR5-47<SUP>T</SUP> (98.0 %), <I>Rhodanobacter soli</I> DCY45<SUP>T</SUP> (97.9 %), <I>Rhodanobacter terrae</I> GP18-1<SUP>T</SUP> (97.9 %) and <I>Dyella ginsengisoli</I> Gsoil 3046<SUP>T</SUP> (97.7 %). The DNA G+C content was 65.2 mol%. In DNA–DNA hybridization, the DNA relatedness levels between strain THG-DD7<SUP>T</SUP> and its closest phylogenetically neighbours were below 40.0 %. The predominant isoprenoid quinone was ubiquinone Q-8. The major polar lipids were diphosphatidylglycerol, phosphtidylethanolamine, phosphatidyl-<I>N</I>-methylethanolamine and phosphatidylglycerol. The major fatty acids were iso-C<SUB>15 : 0</SUB>, iso-C<SUB>16 : 0</SUB>, iso-C<SUB>17 : 0</SUB>, anteiso-C<SUB>15 : 0</SUB> and iso-C<SUB>17 : 1</SUB>ω9<I>c</I> and/or C<SUB>16 : 0</SUB> 10-methyl (summed feature 9). These data supported the affiliation of strain THG-DD7<SUP>T</SUP> to the genus <I>Rhodanobacter</I> . The results of physiological and biochemical tests enabled strain THG-DD7<SUP>T</SUP> to be differentiated genotypically and phenotypically from the species of the genus <I>Rhodanobacter</I> with validly published names. Therefore, the novel isolate represents a novel species, for which the name <I>Rhodanobacter koreensis</I> sp. nov. is proposed. The type strain is THG-DD7<SUP>T</SUP> ( = KACC 17650<SUP>T</SUP> = JCM 19614<SUP>T</SUP>).</P>
-
ScienceON
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
