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RISS 인기검색어
- 검색키워드
- 저자 : Sivadasan, Sangeetha Madambithara (검색결과 5 건)
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Generation of single stranded DNA with selective affinity to bovine spermatozoa
Vinod, Sivadasan Pathiyil,Vignesh, Rajamani,Priyanka, Mani,Tirumurugaan, Krishnaswamy Gopalan,Sivaselvam, Salem Nagalingam,Raj, Gopal Dhinakar Asian Australasian Association of Animal Productio 2021 Animal Bioscience Vol.34 No.10
Objective: This study was conducted to generate single stranded DNA oligonucleotides with selective affinity to bovine spermatozoa, assess its binding potential and explore its potential utility in trapping spermatozoa from suspensions. Methods: A combinatorial library of 94 mer long oligonucleotide was used for systematic evolution of ligands by exponential enrichment (SELEX) with bovine spermatozoa. The amplicons from sixth and seventh rounds of SELEX were sequenced, and the reads were clustered employing cluster database at high identity with tolerance (CD-HIT) and FASTAptamer. The enriched nucleotides were predicted for secondary structures by Mfold, motifs by Multiple Em for Motif Elicitation and 5' labelled with biotin/6-FAM to determine the binding potential and binding pattern. Results: We generated 14.1 and 17.7 million reads from sixth and seventh rounds of SELEX respectively to bovine spermatozoa. The CD-HIT clustered 78,098 and 21,196 reads in the top ten clusters and FASTAptamer identified 2,195 and 4,405 unique sequences in the top three clusters from the sixth and seventh rounds, respectively. The identified oligonucleotides formed secondary structures with delta G values between -1.17 to -26.18 kcal/mol indicating varied stability. Confocal imaging with the oligonucleotides from the seventh round revealed different patterns of binding to bovine spermatozoa (fluorescence of the whole head, spot of fluorescence in head and mid- piece and tail). Use of a 5'-biotin tagged oligonucleotide from the sixth round at 100 pmol with 4×10<sup>6</sup> spermatozoa could trap almost 80% from the suspension. Conclusion: The binding patterns and ability of the identified oligonucleotides confirms successful optimization of the SELEX process and generation of aptamers to bovine spermatozoa. These oligonucleotides provide a quick approach for selective capture of spermatozoa from complex samples. Future SELEX rounds with X- or Y- enriched sperm suspension will be used to generate oligonucleotides that bind to spermatozoa of a specific sex type.
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Dubey, Saurabh,Maiti, Biswajit,Kim, Sung-Hyun,Sivadasan, Sangeetha Madambithara,Kannimuthu, Dhamotharan,Pandey, Pramod Kumar,Girisha, Shivani Kallappa,Mutoloki, Stephen,Chen, Shih-Chu,Evensen, Øystein Blackwell Scientific Publications 2019 Journal of fish diseases Vol.42 No.6
<P> The genus <I>Edwardsiella</I> is one of the major causes of fish diseases globally. Herein, we examined 37 isolates from ten different fish species from India, South Korea and Taiwan to gain insight into their phenotypic and genotypic properties, of which 30 were characterized as <I>E. tarda</I> with phenotypic homology estimated at 85.71% based on API-20E biochemical tests. Genotyping using 16S rRNA put all isolates together with <I>E. anguillarum, E. hoshinae, E. tarda, E. piscicida and E. ictaluri</I> reference strains in a monophyletic group. In contrast, the gyrB phylogenetic tree clearly separated <I>E. ictaluri, E. tarda and E. hoshinae</I> reference strains from our isolates and put our isolates into two groups with group I being homologous with the <I>E. anguillarum</I> reference strain while group II was homologous with the <I>E. piscicida</I> reference strain. Hence, our findings point to <I>E. piscicida</I> and <I>E. anguillarum</I> as species infecting different fish species in Asia. Homology of the ompW protein suggested that strains with broad protective coverage could be identified as vaccine candidates. This study underscores the importance of combining genotyping with phenotyping for valid species classification. In addition, it accentuates the importance of phylogenetic comparison of bacterial antigens for identification of potential vaccine candidates. </P>
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Tongue-lip adhesion in Pierre Robin sequence
K. S. Krishna Kumar,Suresh Vylopilli,Anand Sivadasan,Ajit Kumar Pati,Saju Narayanan,Santhy Mohanachandran Nair 대한구강악안면외과학회 2016 대한구강악안면외과학회지 Vol.42 No.1
Patients with Pierre Robin sequence exhibit varying degrees of airway obstruction and feeding difficulty. In some patients, airway obstruction may be profound, warranting surgical intervention to maintain a patent airway. The purpose of this article is to highlight the advantages of the tongue-lip adhesion procedure for the management of airway obstruction in such patients compared to the currently available options.
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Dynamic smile reanimation in facial nerve palsy
Krishnakumar Krishnan Santha,Subin Joseph,Sameer Latheef,Saju Narayanan,Santhy Mohanachandran Nair,Bibilash Babu,Anand Sivadasan,Srivatsa Manjunath Shet,Rajesh Vardhan Pydi,Ajit Pati,Srikant Aruna Sam 대한구강악안면외과학회 2020 대한구강악안면외과학회지 Vol.46 No.2
Objectives: Long-term facial paralysis results in degeneration of the distal nerve segment and atrophy of the supplied muscles. Options for these patients include free muscle transfer, temporalis myoplasty, and botulinum toxin injections for smile reanimation. In this study we aimed to evaluate the subjective and objective outcomes of these procedures. Materials and Methods: In our study, we retrospectively analyzed smile symmetry in patients with facial palsy (n=8) who underwent facial reanimation procedures. Results: Subjective analysis showed high satisfaction in seven out of eight patients. Objective analysis showed statistically significant improvement postoperatively in both vertical and horizontal smile symmetry at rest and during maximum smile (P<0.001). Conclusion: Choosing the ideal procedure for the patients is the most critical aspect for facial reanimation. Though free muscle transfer is considered gold standard procedure, temporalis myoplasty also gives satisfactory results. Residual synkinesis which can lead to disturbing aesthetic deformity can be effectively treated with botulinum toxin.
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Neural Correlates of Urinary Retention in Lateral Medullary Infarction
Appaswamy Thirumal Prabhakar,Atif Shaikh Iqbal Ahmed,,Aditya Vijayakrishnan Nair,Vivek Mathew,Sanjith Aaron,Ajith Sivadasan,Mathew Alexander 대한배뇨장애요실금학회 2019 International Neurourology Journal Vol.23 No.3
Purpose: The brainstem plays an important role in the control of micturition, and brainstem strokes are known to present with micturition dysfunction. Micturition dysfunction in cases of lateral medullary infarction (LMI) is uncommon, but often manifests as urinary retention. In this study, we investigated the neuro-anatomical correlates of urinary retention in patients with LMI. Methods: This was a hospital-based retrospective study conducted in the neurology unit of a quaternary-level teaching hospital. Inpatient records from January 2008 to May 2018 were searched using a computerized database. Cases of isolated LMI were identified and those with micturition dysfunction were reviewed. MRI brain images of all patients were viewed, and individual lesions were mapped onto the Montreal Neurological Institute (MNI) space manually using MRIcron. Nonparametric mapping toolbox software was used for voxel-based lesion-symptom analysis. The Liebermeister test was used for statistical analysis, and the resultant statistical map was displayed on the MNI template using MRIcron. Results: During the study period, 31 patients with isolated LMI were identified. Their mean age was 48 years and 28 (90%) were male. Six of these patients (19%) developed micturition dysfunction. All 6 patients had urinary retention and 1 patient each had urge incontinence and overflow incontinence. In patients with LMI, the lateral tegmentum of the medulla showed a significant association with urinary retention. Conclusions: In patients with isolated LMI, we postulate that disruption of the descending pathway from the pontine micturition centre to the sacral spinal cord at the level of the lateral tegmentum results in urinary retention.
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