Photo Physical Studies of PVP Arrested ZnS Quantum Dots

Ashutosh Kumar Shahi,Bishnu Kumar Pandey,Bheeshma Pratap Singh,Bipin Kumar Gupta,Sukhvir Singh,Ram Gopal 대한금속·재료학회 2017 ELECTRONIC MATERIALS LETTERS Vol.13 No.2

Monodispersed polyvinylpyrrolidone (PVP) arrested ZnS quantumdots (QDs) having diameter in range ~2-5 nm are synthesized by acolloidal precipitation method using PVP as the stabilizing agent. X-ray diffraction (XRD), transmission electron microscopy (TEM),high resolution transmission electron microscopy (HRTEM),selective area electron diffraction (SAED) and Fourier transforminfrared (FT-IR) spectroscopy are probed to investigate thestructural information. The optical properties are studied usingdiffuse UV-visible reflectance and photoluminescence (PL)spectroscopy techniques. TEM images as well as XRD reflectionpeak broadening indicate the nanometer size particles formationwith cubic (sphalerite) phase within the polymer matrix. Opticalabsorbance studies reveal an excitonic peak at around ~310 nmdictates the effect of quantum confinement effect in the ZnS QDs. PL emission spectra for ZnS QDs in PVP exhibit four emissionpeaks at ~382 nm, ~414 nm, ~480 nm and ~527 nm are observed. These excitonic emissions from ZnS QDs are caused by theinterstitial sulfur/Zn vacancies and surface states.

Genome-wide identification and analysis of Subtilisin-like serine protease gene family in banana (Musa accuminta L.) and their expression under abiotic stresses

Purwar Shalini,Chugh Vishal,Singh Poornima,Srivastava Akhilesh Kumar,Singh Akhilesh Kumar,Mishra Akhilesh Chandra,Singh Ashutosh,Singh Chandra Mohan 한국식물생명공학회 2024 Plant biotechnology reports Vol.18 No.1

Subtilisin-like serine proteases (SBT), a serine proteolytic enzymes play an important role in plant growth function and during different stresses responses. The systematic analysis of the SBT gene family in Musa acuminate (MaSBT) has been done and their responses to abiotic stresses in banana variety cv. G-9 were also analyzed. Total of 67 MaSBT genes were identified and based on phylogeny these were grouped into five districted subgroups. Cis-acting element analysis indicated that almost all MaSBT promoters contain regulatory elementary related to growth and development, hormonal regulation, and stress responses. The gene structure and domain analysis showed a maximum of seventeen exons and four functional domains in MaSBT. The 42 orthologous genes, 07 MaSBT paralogous genes were also identified through synteny analysis. The Ka /Ks study indicated that four MaSBT paralogous gene pairs were tandemly duplicated, while the other three were segmental duplications. Further, the expression pattern via RNA-seq data revealed that MaSBTs exhibited differential expres- sion specifically in response to the abiotic stress of low nitrogen. and also during the flowering time. The MaSBT-1.7 gene was found involved in the response to salt stress and flowering. These findings establish a cornerstone for future research on banana's salt stress mechanism. The study offers valuable insights into SBT encoding genes, shedding light on their roles in growth, development, and abiotic stress responses

Diagnostic Evaluation of Non-Interpretable Results Associated with rpoB Gene in Genotype MTBDRplus Ver 2.0

( Binit Kumar Singh ),( Rohini Sharma ),( Parul Kodan ),( Manish Soneja ),( Pankaj Jorwal ),( Neeraj Nischal ),( Ashutosh Biswas ),( Sanjay Sarin ),( Ranjani Ramachandran ),( Naveet Wig ) 대한결핵 및 호흡기학회 2020 Tuberculosis and Respiratory Diseases Vol.83 No.4

Background: Line probe assay (LPA) is standard diagnostic tool to detect multidrug resistant tuberculosis. Noninterpretable (NI) results in LPA (complete missing or light wild-type 3 and 8 bands with no mutation band in rpoB gene region) poses a diagnostic challenge. Methods: Sputum samples obtained between October 2016 and July 2017 at the Intermediate Reference Laboratory, All India Institute of Medical Sciences Hospital, New Delhi, India were screened. Smear-positive and smear-negative culturepositive specimens were subjected to LPA Genotype MTBDRplus Ver 2.0. Smear-negative with culture-negative and culture contamination were excluded. LPA NI samples were subjected to phenotypic drug susceptibility testing (pDST) using MGIT-960 and sequencing. Results: A total of 1,614 sputum specimens were screened and 1,340 were included for the study (smear-positive [n=1,188] and smear-negative culture-positive [n=152]). LPA demonstrated 1,306 (97.5%) valid results with TUB (Mycobacterium tuberculosis) band, 24 (1.8%) NI, three (0.2%) valid results without TUB band, and seven (0.5%) invalid results. Among the NI results, 22 isolates (91.7%) were found to be rifampicin (RIF) resistant and two (8.3%) were RIF sensitive in the pDST. Sequencing revealed that rpoB mutations were noted in all 22 cases with RIF resistance, whereas the remaining two cases had wild-type strains. Of the 22 cases with rpoB mutations, the most frequent mutation was S531W (n=10, 45.5%), followed by S531F (n=6, 27.2%), L530P (n=2, 9.1%), A532V (n=2, 9.1%), and L533P (n=2, 9.1%). Conclusion: The present study showed that the results of the Genotype MTBDRplus assay were NI in a small proportion of isolates. pDST and rpoB sequencing were useful in elucidating the cause and clinical meaning of the NI results.

Comparative expression analysis of defense-related genes in both transgenic and nontransgenic Brassica juncea (var.) Varuna harbouring overexpressed MAPK3 gene in response to infection by Albugo candida

Modak Annayasa,Singh Brij Raj,Dubey Ashutosh,Tewari A. K.,Taj Gohar 한국작물학회 2022 Journal of crop science and biotechnology Vol.25 No.1

White rust, a devastating disease of Brassicaceae family, causes nearly 60% reduction in yield of Brassica juncea (Indian mustard). This dreadful disease is caused by oomycetes fungi Albugo candida. A comparative expression analysis of MAPKs cascade and some of defense-related genes in nontransgenic and transgenic (harbouring overexpressed MAPK3) cultivar of Brassica juncea were done to reveal the defense signalling pathway against biotrophic fungi Albugo candida. Disease indexing was also done to compare the disease severity in both transgenic and nontransgenic Brassica juncea cultivars. Lower disease index in transgenic Brassica suggests its tolerance to Albugo candida. Higher expression level of MAPK6 in comparison to MAPK3 in the transgenic Brassica indicates that MAPK6 mimics a portion of MAPK3 signalling pathway and work in a direct cascade for production of defense-related proteins. Overexpression of MAPK3 gene was observed in downregulating the expression of MAPK4 in transgenic Brassica juncea. The expression of two important transcription factors, such as WRKY33 and WRKY29 were also analyzed in the present study and were found to be higher in transgenic plant overexpressing MAPK3. The expression of three defense-related genes (OASTL-B, ACD2 and CSD2) was found to be increased in transgenic plants harbouring overexpressed MAPK3 gene; however, expression level of CYP20-3 was found higher in nontransgenic Brassica plant in response to pathogen infection. These four defense-related genes were also subjected to in silico studies, such as secondary structure, tertiary structure and putative phosphorylation sites prediction in addition to protein–protein interaction determination with potential MAPKs cascade and WRKY29, WRKY33.

Resource-efficient load-balancing framework for cloud data center networks

Kumar, Jitendra,Singh, Ashutosh Kumar,Mohan, Anand Electronics and Telecommunications Research Instit 2021 ETRI Journal Vol.43 No.1

Cloud computing has drastically reduced the price of computing resources through the use of virtualized resources that are shared among users. However, the established large cloud data centers have a large carbon footprint owing to their excessive power consumption. Inefficiency in resource utilization and power consumption results in the low fiscal gain of service providers. Therefore, data centers should adopt an effective resource-management approach. In this paper, we present a novel load-balancing framework with the objective of minimizing the operational cost of data centers through improved resource utilization. The framework utilizes a modified genetic algorithm for realizing the optimal allocation of virtual machines (VMs) over physical machines. The experimental results demonstrate that the proposed framework improves the resource utilization by up to 45.21%, 84.49%, 119.93%, and 113.96% over a recent and three other standard heuristics-based VM placement approaches.

Duty ratio control technique with torque ripple minimization for induction motor‑based electric vehicle applications

Sudhir Sharma,Bhoopendra Singh,Ashutosh Datar 전력전자학회 2023 JOURNAL OF POWER ELECTRONICS Vol.23 No.4

DTC drives are utilized in modern electrical vehicles. This paper proposed a blend of the conventional direct torque control (DTC) and a duty ratio control technique known as the modified duty ratio control for torque ripple reduction. In this modified technique, voltage vector selection is based on the conventional DTC technique, which utilizes six entire voltage vectors and two zero vectors. The torque error modifies the duty ratio of the voltage vector. In the presented technique, the magnitude of the torque error is the controlling variable for the modification of the duty ratio of the voltage vectors. Depending on the magnitude of the torque error, a duty ratio-modulating factor is generated from a lookup table, which is then multiplied by the duty ratio for any of the selected voltage vectors. Thus, improved performance in terms of low torque and current ripples is achieved from a DTC drive by a control strategy with less complexity, which preserves the simplicity of switching to table-based traditional DTC drives. Simulation and experimental results validate the proposed switching strategy.

Optimization and evaluation of multigrain gluten-enriched instant noodles

Savita Rani,Rakhi Singh,Barjinder Pal Kaur,Ashutosh Upadhyay,Dinkar B. Kamble 한국응용생명화학회 2018 Applied Biological Chemistry (Appl Biol Chem) Vol.61 No.5

Central composite design was employed to optimize the cooking, textural and overall acceptability score of the instant dried noodles prepared with multigrain flour and gluten incorporation. Sorghum flour (X1, 10–50%), soy flour (X2, 10–20%) and gluten (X3, 2–4%) were the independent variables investigated with respect to five response variables including cooking time (Y1), cooked weight (Y2), cooking loss (Y3), hardness (Y4) and overall acceptability (Y5). The optimum level was found to be 24.61% sorghum, 13.23% soy and 2.95% gluten resulting in cooking time = 9 ± 0.60 min, cooked weight = 17.30 ± 0.17 g, cooking loss = 11.46 ± 0.64 g/100 g, hardness = 36.65 ± 3.2 N with overall acceptability score of 7.3 ± 0.71, respectively. Optimized noodles showed higher ash (3.40 ± 0.11%), protein (16.63 ± 0.55%), fiber (4.78 ± 0.04%) as well as iron content (4.53 ± 0.02 mg/ 100 g) than the control (0.83 ± 0.02%, 13.13 ± 0.84%, 0.00 and 2.38 mg/100 g) and Maggie noodles (3.19 ± 0.01%, 10.53 ± 0.30%, 0.41 ± 0.50% and 0.22 ± 0.00 mg/100 g) made with refined wheat flour. Optimized noodles also revealed good total phenolic content (84.57 ± 1.42 mg GAE/100 g DW) and 1,1-diphenyl- 2-picrylhydrazyl scavenging activity (19.64 ± 0.20%). Hence, optimized noodles have substantial potential as a protein–fiber-rich complementary food to improve the nutrient delivery of mid-day meal scheme and satisfying the protein requirement of primary class children (12 g/ child/day) as laid down by MHRD (India) under the scheme.

Cardiac arrest in the prone position caused by central venous cannulation-induced cardiac tamponade

Nitasha Mishra,Shalendra Singh,Anirudh Elayat,Ashutosh Kaushal 대한마취통증의학회 2019 Korean Journal of Anesthesiology Vol.72 No.4

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Challenges, Configuration, Control, and Scope of DC Microgrid Systems: A Review

Maurya Rinki,Prakash Surya,Singh Ashutosh Kumar 대한전기학회 2023 Journal of Electrical Engineering & Technology Vol.18 No.3

Managing natural resources and air pollution has been challenging for humans for quite a long time. A severe manifestation of natural resource mismanagement has been in the form of CO2 emissions from smoke bellowing thermal power plants (TPPs). Besides evoking global warming, the TPPs also foster limited coal reserve reduction with the increasing electricity demand. The ongoing scenario may lead to severe catastrophes until efficient plans for natural resource management are prepared. Many researchers have suggested DC microgrid as a credible alternative for power generation, significantly reducing carbon emissions. Efficient control strategies have brought microgrid technology to the level of other generation sources in terms of system reliability and efficiency. Renewable energy resources can be implemented as a safe, low voltage (< 50 V) local DC microgrid for DC load, reducing the converter requirement with low transmission losses and overcoming the scarcity of electricity. Seamless integration of solar-PV systems and batteries into the DC microgrid will increase the predominance of clean electricity in the modern power system. It will alleviate the air pollution problems generated by TPPs and sequels to enable natural resource management effectively. In light of the above facts, this paper presents a detailed survey on the challenges, configuration, control, and scope of DC microgrid systems. Various predominant configurations, recent advances in control strategies, operation challenges, and DC microgrid commissioning have been discussed. Moreover, a case study illustrating the benefits of DC systems is presented along with the energy-saving analysis compared with AC systems.

Intestinal Parasitosis in Relation to Anti-Retroviral Therapy, CD4<SUP>+</SUP> T-cell Count and Diarrhea in HIV Patients

Shehla Khalil,Bijay Ranjan Mirdha,Sanjeev Sinha,Ashutosh Panda,Yogita Singh,Anju Joseph,Manorama Deb 대한기생충학열대의학회 2015 The Korean Journal of Parasitology Vol.53 No.6