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- 저자 : Shiming Gao (검색결과 2 건)
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Quanxin Gao,Yanfeng Yue,Minghua Min,Shiming Peng,Zhaohong Shi,Jinbo Wang,Tao Zhang 한국통합생물학회 2018 Animal cells and systems Vol.22 No.4
The Kelp grouper Epinephelus moara is one of the most widely consumed and economically important marine fish in China. The species can tolerate a wide range of salinity, but genomic resources are not available, and the molecular mechanisms underlying adaptation to salinity at the transcriptomic level remain largely unclear. In this study, the transcriptomic responses of the liver of E. moara under low salinity were investigated using the Illumina digital gene expression system. After de novo assembly, 499,356 transcripts were generated and contributed 445,068 unigenes. A total of 14, 19, 33 and 3101 genes were differentially expressed following exposure to low salinity stress for 2, 6, 24 and 48 h, respectively. Only two genes were differentially expressed in all groups. Four genes related to metabolism and ambient salinity adaption were randomly selected to validate the differentially expressed genes (DEGs) by real-time PCR. Gene Ontology (GO) and Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathway enrichment analysis were used to analyse the functional significance of DEGs, including those responding to salinity through diverse biological processes, cellular components, molecular functions, and pathways associated with metabolic and osmotic responses. This work provides new insight into the response to salinity challenges in E. moara, and the findings expand our knowledge of the molecular basis of metabolic regulation mechanisms in this species. Additionally, the transcriptional data provide a valuable resource for future molecular and genetic studies on E. moara.
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Analysis on the safe distance between shield tunnel through sand stratum and underlying karst cave
Wei Wan,Shiming Gao,Lingfeng Liu,Wushuang Wen,Ping Li,Jianping Chen 한국자원공학회 2019 Geosystem engineering Vol.22 No.2
When a subway tunnel passes through a karst geological area, in order to ensure the stability of the tunnel, the safe distance between the tunnel and karst cave needs to be researched. This article is based on the construction project of Wuhan metro line 6 from Qianjincun station to Mayinglu station. In view of the geological condition of overlying sand and underlying limestone, by using numerical simulation and theoretical calculation and selecting a characteristic section, the influence law and degree of karst cave span, height-span ratio, and filling degree on the safe thickness of karst cave roof are studied and a forecasting model of the safe thickness of karst cave roof is established. Combined with dichotomy and numerical simulation, the critical horizontal safe distance beyond which cave collapse does not cause tunnel instability is calculated. The results show that: (1) the safe thickness of karst cave roof increases linearly with the increase of karst cave span, and decreases with the increase of height-span ratio and filling degree; (2) among the three factors, the effect of karst cave span is the most significant, the filling degree of karst cave is the second, and the influence of height-span ratio of karst cave is the weakest; (3) the proposed forecasting model passes the significance test, indicating that the model is very reasonable; and (4) the critical horizontal safe distance from the tunnel border to the karst cave boundary is 22.4 m. The research results can provide guidance for the design and construction of shield tunnels and karst cave treatment in karst areas.
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