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Sequence Analysis of the Nuclear Matrix - Associated DNA Segments
Lee, Sang Eun,Park, Sang Dai,Lee, Chee Gun,Kim, Chan Kil 한국유전학회 1988 Genes & Genomics Vol.10 No.4
Nuclear matrix isolated from synchronized LP1-1 cells at G1/S boundary digested with DNase 1 to separate a nuclear-matrix(N.M)-associated DNA segments from the rest Through the cloning of the N.M-associated DNA segments (300-500 base pair in size) into a pUC18 vector, 800 clones (named as pMRs) were selected with X-Gal/IPTG and amphiciline. Putative clones harboring replication origin sequence were sequenced and characterized. We found that pMR15 and 16 act as a good template for origins of DNA replication in in vitro replication assay. Furthermore, pMR15 shows A/T richness and has a palindromic sequence. However, pMR62 shows G/C richness (65.7%) and has a homologous sequence to that of Adenovirus replication origin i.e. TGAPyGCC AGG.
Lee, Chae-Kyung,Park, Jeong-Kil,Kim, Hyeon-Cheon,Woo, Sung-Gwan,Kim, Kwang-Hoon,Son, Kwon,Hur, Bock 대한치과보존학회 2007 Restorative Dentistry & Endodontics Vol.32 No.1
이 연구의 목적은 쐐기형 비우식성 5급와동을 복합레진으로 수복하기 전, 후에 과도한 교합력에 의한 응력분포 변화를 비교연구하기 위함이었다. 발치된 상악 제2소구치를 이용하여 쐐기형 비우식성 치경부병소를 가진 3차원 유한요소모형을 제작한 후 탄성계수가 서로 다른 혼합형 복합레진과 흐름성 복합레진으로 각각 충전하고 이때의 상아질 접착제의 두께는 40 ㎛로 하였다. 협측교두와 설측교두에 500 N의 하중을 각각 가한 후 ANSYS 프로그램을 이용하여 주응력분석법으로 병소의 심부와 와동 수직 벽의 응력분포를 비교하여 다음과 같은 결과를 얻었다. 1. 협측교두에 하중이 가해지면 병소에 압축응력이 나타나고, 설측교두에 가해지면 인장응력이 나타난다. 두 가지 하중에서 모두 병소의 근심 끝 부위와 인접한 백악법랑경계 그리 고 병소의 심부에 응력 이 집중되었다. 2. 응력의 집중을 보였던 병소의 근심부근과 심부는 수복 후 응력이 많이 감소하였으며 대신 다른 부위에서는 응력이 약간 증가하였다. 3. 병소의 근심부위와 심부는 흐름형 복합레진으로 수복하였을 때 보다 혼합형 복합레진으로 수복하였을 때 응력이 더 많이 감소하였다. The purpose of this study was to investigate the effects of composite resin restorations on the stress distribution of notch shaped noncarious cervical lesion using three-dimensional (3D) finite element analysis (FEA). Extracted maxillary second premolar was scanned serially with Micro-CT (SkyScan1072; SkyScan, Aartselaar, Belgium). The 3D images were processed by 3D-DOCTOR (Able Software Co., Lexington, MA, USA). ANSYS (Swanson Analysis Systems, Inc., Houston, USA) was used to mesh and analyze 3D FE model. Notch shaped cavity was filled with hybrid or flowable resin and each restoration was simulated with adhesive layer thickness (40 ㎛). A static load of 500 N was applied on a point load condition at buccal cusp (loading A) and palatal cusp (loading B). The principal stresses in the lesion apex (internal line angle of cavity) and middle vertical wall were analyzed using ANSYS. The results were as follows 1. Under loading A, compressive stress is created in the unrestored and restored cavity. Under loading B. tensile stress is created. And the peak stress concentration is seen at near mesial corner of the cavity under each load condition. 2. Compared to the unrestored cavity, the principal stresses at the cemeto-enamel junction (CEJ) and internal line angle of the cavity were more reduced in the restored cavity on both load conditions. 3. In teeth restored with hybrid composite, the principal stresses at the CEJ and internal line angle of the cavity were more reduced than flowable resin.
Lee, Beom Suk,Park, Kyeongsoon,Park, Sangjin,Kim, Gui Chul,Kim, Hyo Jung,Lee, Sangjoo,Kil, Heeseup,Oh, Seung Jun,Chi, Daeyoon,Kim, Kwangmeyung,Choi, KuiWon,Kwon, Ick Chan,Kim, Sang Yoon Elsevier 2010 Journal of controlled release Vol.147 No.2
<P><B>Abstract</B></P><P>The better understanding of polymeric nanoparticles as a drug delivery carrier is a decisive factor to get more efficient therapeutic response <I>in vivo</I>. Here, we report the non-invasive imaging of bare polymeric nanoparticles and drug-loaded polymeric nanoparticles to evaluate biodistribution in tumor bearing mice. To make nano-sized drug delivery carrier, glycol chitosan was modified with different degrees of hydrophobic N-acetyl histidine (NAcHis-GC-1, -2, and -3). The biodistribution of polymeric nanoparticles and drug was confirmed by using gamma camera with <SUP>131</SUP>I-labeled NAcHis-GC and <SUP>131</SUP>I-labeled doxorubicin (DOX) and by using <I>in vivo</I> live animal imaging with near-infrared fluorescence Cy5.5-labeled NAcHis-GC. Among bare nanoparticles, NAcHis-GC3 (7.8% NAcHis content) showed much higher tumor targeting efficiency than NAcHis-GC1 (3.3% NAcHis content) and NAcHis-GC2 (6.8% NAcHis content). In contrast, for drug-loaded nanoparticles, DOX-NAcHis-GC1 displayed two-fold higher tumor targeting property than DOX-NAcHis-GC3. These data imply that the biodistribution and tumor targeting efficiency between bare and drug-loaded nanoparticles may be greatly different. Therapeutic responses for NAcHis-GC nanoparticles after drug loading were also evaluated. In xenograft animal model, we could find out that DOX-NAcHis-GC1 with higher tumor targeting of DOX has more excellent therapeutic effect than DOX-NAcHis-GC3 and free DOX. These results mean that the hydrophobic core stability might be a critical factor for tumor targeting efficiency of nanoparticles. The present study indicates that by using molecular imaging, we can select more appropriate nanoparticles with the highest tumor targeting properties, leading to exerting more excellent therapeutic results in cancer therapy.</P> <P><B>Graphical Abstract</B></P><P><ce:figure id='f0035'></ce:figure></P>
Lee, Jin-Hee,Byun, Do-Sun,Lee, Min-Goo,Ryu, Byung-Kyu,Kang, Min-Ju,Chae, Kwon-Seok,Lee, Kil Yeon,Kim, Hyo-Jong,Park, Heonyong,Chi, Sung-Gil Wiley Subscription Services, Inc., A Wiley Company 2008 International journal of cancer: Journal internati Vol.122 No.7
<P>hSRBC is a putative tumor suppressor located at 11p15.4, at which frequent genomic loss has been observed in several human malignancies. To explore the candidacy of hSRBC as a suppressor of gastric tumorigenesis, we analyzed the expression and mutation status of hSRBC in gastric tissues and cell lines. hSRBC transcript was expressed in all normal and benign tumor tissues examined, but undetectable or very low in 73% (11/15) cancer cell lines and 41% (46/111) primary tumors. Loss or reduction of hSRBC expression was tumor-specific and correlated with stage and grade of tumors. While allelic loss or somatic mutations of the gene were infrequent, its expression was restored in tumor cells by 5-aza-2′-deoxycytidine treatment and aberrant hypermethylation of 23 CpG sites in the promoter region showed a tight association with altered expression. Transient or stable expression of hSRBC led to a G<SUB>1</SUB> cell cycle arrest and apoptosis of tumor cells, and strongly suppresses colony forming ability and xenograft tumor growth. In addition, hSRBC elevated apoptotic sensitivity of tumor cells to genotoxic agents, such as 5-FU, etoposide and ultraviolet. Interestingly, hSRBC increased the protein stability of p53 and expression of p53 target genes, such as p21<SUP>Waf1</SUP>, PUMA and NOXA, while hSRBC-mediated cell cycle arrest and apoptosis were abolished by blockade of p53 function. Our findings suggest that hSRBC is a novel tumor suppressor whose epigenetic inactivation contributes to the malignant progression of gastric tumors, in part, through attenuated p53 response to stresses. © 2007 Wiley-Liss, Inc.</P>
Lee, Jong Il,Cho, Sung Suk,Kil, Eui-Joon,Kwon, Suk-Tae Elsevier 2010 Enzyme and microbial technology Vol.47 No.4
<P><B>Abstract</B></P><P>The biochemical properties of the <I>Thermococcus pacificus</I> (<I>Tpa</I>) DNA polymerase were determined to evaluate its feasibility for use in polymerase chain reaction (PCR) application. The <I>Tpa</I> DNA polymerase gene was expressed under the control of the T7<I>lac</I> promoter in the pET-22b(+) plasmid in <I>Escherichia coli</I> BL21-CodonPlus(DE3)-RIL. The enzyme was then purified by heat treatment followed by two steps of column chromatography after which the optimum pH and temperature of the enzyme were determined to be pH 7.5 and 75°C. The optimal PCR buffer for <I>Tpa</I> DNA polymerase consisted of 50mM Tris–HCl (pH 8.4), 4mM MgCl<SUB>2</SUB>, and 10mM KCl. <I>Tpa</I> DNA polymerase performed significantly more efficiently in PCR amplification than <I>Taq</I> or <I>Pfu</I> DNA polymerase. By fusing the <I>Sulfolobus solfataricus</I> DNA binding protein Sso7d to <I>Tpa</I> DNA polymerase, we obtained a fusion polymerase which exhibits profound advantages over unmodified <I>Tpa</I> DNA polymerase in PCR applications. <I>Tpa</I> DNA polymerase (2.04×10<SUP>−6</SUP>) and <I>Tpa</I>-S DNA polymerase (2.20×10<SUP>−6</SUP>) revealed a 5-fold higher fidelity than <I>Taq</I> DNA polymerase (12.13×10<SUP>−6</SUP>).</P>