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Characterization of DNJ production for large-scale fermentation of mulberry leaf
Kwon, O-Chul,Ju, Wan-Taek,Kim, Hyun-Bok,Sung, Gyoo-Byung,Kim, Yong-Soon Korean Society of Sericultural Science 2017 International Journal of Industrial Entomology Vol.35 No.2
Mulberry leaves containing 1-deoxynojirimycin (DNJ) known to be a strong inhibitory effect for ${\alpha}-glucosidase$. Thus, DNJ has been recognized as a potentially important source for prevent or treat hyperglycemia. More effective method for the DNJ high-production is needed because DNJ content of natural mulberry leaf are as low as 0.1%. Many researchers have studied for the DNJ high-production in mulberry leaves such as the harvest season, fermentation using microorganisms, optimal culture conditions, and optimal extraction conditions. In order to provide for useful data that is anticipated at the level of industrial scale, we investigated ${\alpha}-glucosidase$ inhibitory activity, pH value and DNJ content in large-scale based on the optimal culture conditions for mulberry leaf fermentation of small-scale in our previous study. The ${\alpha}-glucosidase$ inhibitory activity, pH value, and DNJ content in this study were measured from the mulberry leaf fermentation broth for 7 days. During mulberry leaf fermentation, the ${\alpha}-glucosidase$ inhibitory activity and DNJ content was increased until 2 to 4 days, but after 4 day was decreased. The pH value showed a decreasing trend up to 2 day, and little changes in 2 to 4 days. However, the pH was started to increase after 4 days.
(Sung Hyun Kwon),(Duk Soo Choi),(Dae Chul Cho) 한국물환경학회 2000 한국물환경학회지 Vol.16 No.1
Biodegradability of pentachlorophenol and its biological kinetic parameters were investigated using a chemical oxygen demand (COD) analysis. The experiments were performed in continuous stirred tank reactors with synthetic wastewater containing 30 and 60㎎/L of pentachlorophenol (PCP) and other carbon sources. Mean cell residence time (MCRT) and hydraulic residence time (HRT) were maintained from 4.8 to 26.2 days and 4 to 17 hours, respectively. While synthetic wastewater containing 30 ㎎/L of PCP showed over 90% S-COD removal efficiencies except at the 4.8 and 20.3 days of MCRT, 55% to 75% removal efficiencies were observed for 60 ㎎/L of PCP concentration. The respective values of Y and k_d were 0.15 and 0.010 day^(-1) for 30 ㎎/L PCP (r²= 0.90), and 0.13 and 0.014 day^(-1) for 60 ㎎/L PCP (r² = 0.86), respectively. In a substrate inhibition kinetics, Haldane model, the respective values of K_s, K_i and k were 60.8 ㎎ COD/L, 297.5 ㎎ COD/L and 0.93 day^(-1) for 30 ㎎/L PCP (r² = 0.30), and 262.2 ㎎ COD/L, 997.2 ㎎ COD/L and 0.053 day^(-1) for 60 ㎎/L PCP (r² = 0.78), respectively.
Proteome analysis of outer membrane vesicles from a clinical<i>Acinetobacter baumannii</i>isolate
Kwon, Sang-Oh,Gho, Yong Song,Lee, Je Chul,Kim, Seung Il Oxford University Press 2009 FEMS microbiology letters Vol.297 No.2
<P>The secretion of outer membrane vesicles (OMVs) is one of the major mechanisms by which Gram-negative bacteria deliver effector molecules to host cells. Acinetobacter baumannii is an important opportunistic pathogen in hospital-acquired infections, but the secretion system for effector molecules to induce host cell damage has not been characterized. In the present study, we investigated the secretion of OMVs from a clinical A. baumannii isolate and analyzed the comprehensive proteome of A. baumannii-derived OMVs. Acinetobacter baumannii secreted OMVs into the extracellular milieu during in vitro growth. Using 1-DE and LC-MS/MS protein identification and assignment analysis, 132 different proteins associated with OMVs were identified. These proteins were derived from outer membranes (n=26), periplasmic space (n=6), inner membranes (n=8), cytoplasm (n=43), and unknown localization or multiple localization sites (n=49) according to the cell location prediction programs. Among the proteins associated with OMVs, a potent cytotoxic molecule, outer membrane protein A, was highly enriched and several putative virulence-associated proteins were also identified. These results suggest that OMVs from A. baumannii are an important vehicle designed to deliver effector molecules to host cells.</P>
Kwon, O-Chul,Ju, Wan-Taek,Kim, Hyun-Bok,Sung, Gyoo-Byung,Kim, Yong-Soon Korean Society of Sericultural Science 2017 International Journal of Industrial Entomology Vol.34 No.2
Mulberry leaves containing 1-deoxynojirimycin (DNJ) have been recognized as a potentially important source for prevent or treat hyperglycemia. However, DNJ content of natural mulberry leaf are as low as 0.1%. Thus, the most effective method for increasing ${\alpha}$-glucosidase inhibitory activity with the DNJ high-production is needed. In this study, we investigated the influence of ${\alpha}$-glucosidase inhibitory activity according to different pH values (6-9) and inoculation amounts (0.1-0.5%) when Bacillus subtilis cultured on mulberry leaf powder media. We confirmed that ${\alpha}$-glucosidase inhibitory activity was difference according to culture conditions of different pH values, inoculation amounts, and fermentation times. The results of mulberry leaf fermentation according to pH values and inoculation amounts were shown that the optimal conditions for ${\alpha}$-glucosidase inhibitory activity were defined as pH 7 and 9, inoculation amount 0.4%, and incubation until 2 to 4 days. These results can be provided a basic data for the optimal culture conditions increasing ${\alpha}$-glucosidase inhibitory activity from mulberry leaf fermentation.
Sang-Chul Na,Min Chul Chun,Gyuyeon Jang,Hyejin Shin,Young-Sun Kwon,Bo Soo Kang,Keundong Lee,Chansoo Yoon,박배호 한국물리학회 2016 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.68 No.12
The set process in a unipolar resistance switching Pt/NiO/Pt thin film was conducted in two different ways: the current-biased set process (current sweep mode) and the voltage-biased set process (voltage sweep mode). In the current-biased set process, a compliance current setting was not necessary for continuing stable resistance switching. The resistance of the low resistance state, the reset and the set switching parameters were compared in both modes of the set processes. The distributions of the set parameters were found to be effectively reduced in the current-biased set process. These intriguing properties can be attributed to the prevention of an overshoot current during the set transition.
( Sang Chul Shin ),( Jong Yul Roh ),( Hee Jin Shim ),( Soon Kee Kim ),( Chul Su Kim ),( Il Kwon Park ),( Mun Jang Jeon ),( Yeon HO Je ) 한국잠사학회 2005 International Journal of Industrial Entomology Vol.10 No.2
Beauveria bassiana F-101, which has high toxicity toward Acantholyda parki as well as Thecodiplosis japonensis, was an isolate to develop an alternative control system against the major forest pests. Up to now, in B. bassiana, only one pr1 gene has been isolated and characterized. Therefore, we here reported the identification of a prl-like gene, which would be a factor of toxicity from B. bassiana F-101. The oligonucleotides for the amplification of the prl-like gene, were chosen based on the conserved regions of the subtilisin family enzymes, prl genes of B. bassiana and Metarhizium anisopliae, and proteinase K of Tritirachium album. The cloned PCR fragment had 1111bp including 52bp intron. The deduced Prl-like peptide showed a low identity with Prls of entomopathogenic fungi such as B. bassiana Prl (BbPrl) and M. anisopliae Prl (MaPrl) as well as the proteinase K of T. album (TaPrK). Instead, the deduced peptide had a substantially high amino acid sequence identity (>65%) with the serine proteases of Magnaporthe grisea (MgSPM1) and Podospora anserina (PaPspA). These results, therefore, appear to suggest that the putative Prl-like peptide of B. bassiana F-101 belongs to the subtilisin-like serine protease family and may be a novel gene.