인체구강상피세포의 배양조건에 대한 연구

박종수,최희웅,이수관,김수복,고광삼 中央醫學社 1990 中央醫學 Vol.55 No.2

Human epithelial cell cultures were initiated from explants of normal human oral mucosa. Growth parameter and cell type were assessed by DNA concentration and morphology of the cell lines. Tissues were excised and epithelial cells isolated by collagenase digestion. Spermine was used in order to inhibit overgrowth of the epithelial cells by fibroblast. The effects of different medium formulations were compared. Cultures maintained on the same volume of RPMI 1640 and Dulbecco's modified minimum essential medium containing 10 % fetal bovine serum, 10 ?g/ml of insulin, lng/ml of somatomedin and 10 ng/ml of epidermal growth factor showed optimal and maximal growth. For each cultured human oral epithelial cell the DNA content was in the- range of 5.7 pg to 6.6 pg. This culture system provides epithelial cells that can be used for a variety of dental and biochemical studies.

Transthyretin의 Glutathione 자동산화 촉진작용

박종근,정신,김재휴,김수한,강삼석,이제혁,안봉환 대한신경외과학회 1994 Journal of Korean neurosurgical society Vol.23 No.8

When reduced glutathione(GSH) was incubated at neutral pH and at 37°, its concentration decreased slowly with formation of oxidized glutathione(GSSG). Autooxidation of GSH was accelerated by Cu^(2+) and Hg^(2+), but not by other common mono-, di-, and tri-valent cations. Transthyretin was found to stimulate autooxidation of GSH in the presence or absence of Cu^(2+) and Hg^(2+). EDTA inhibited perfectly the autooxidation of GSH regardless of the presence of transthyretin. The stimulating activity of transthyretin was maximal at pH 7.0, declining progressively with increase or decrease of pH from 7.0. Sulfhydryl-blocking agents such as p-hydroxymercuribenzoic acid and Nethylmaleimide markedly inhibited the stimulating activity of transthyretin. Transthyretin stimulated autooxidation of other sulfhydryl compounds such as clithiothreitol and cysteine. However, it did not show a significant effect on autooxidation of sulfhydryl group of egg albumin and eye lens proteins. And transthyretin did not cause any oxidative change to thyroxine(T₄), 3, 5, 3Ltri iodo thyronine(T₃) and 3, 3: 5~triiodothpnine(rT₃) bound to it in the presence of GSH and Cu^(2+). The above results suggest that transthyretin may play a role in regulation of oxidized status of sulfhydryl groups in blood plasma and cerebrospinal fluid

돼지 하악 과두의 해면골에서 유한요소분석법으로 예측한 방향에 따른 탄성율과 3차원 골 미세지표 간의 상관관계

박관수,이삼선,허경회,이원진,허민석,최순철 대한구강악안면방사선학회 2008 Imaging Science in Dentistry Vol.38 No.2

Purpose : To investigate the relationship between 3D bone architectural parameters and direction-related elastic moduli of cancellous bone of mandibular condyle. Materials and Methods : Two micro-pigs (Micro-pig^(R), PWG Genetics Korea) were used. Each pig was about 12 months old and weighing around 44 kg. 31 cylindrical bone specimen were obtained from cancellous bone of condyles for 3D analysis and measured by micro-computed tomography. Six parameters were trabecular thickness (Tb.Th), bone specific surface (BS/BV), percent bone volume (BV/TV), structure model index (SMI), degree of anisotropy (DA) and 3-dimensional fractal dimension (3DFD). Elastic moduli of three orthogonal directions (superiorinferior (SI), medial-lateral (ML), andterior-posterior (AP) direction) were calculated through finite element analysis. Results : Elastic modulus of superior-inferior direction was higher than those of other directions. Elastic moduli of 3 orthogonal directions showed different correlation with 3D architectural parameters. Elastic moduli of SI and ML directions showed significant strong to moderate correlation with BV/TV, SMI and 3DFD. Conclusion : Elastic modulus of cancellous bone of pig mandibular condyle was highest in the SI direction and it was supposed that the change into plate-like structure of trabeculae was mainly affected by increase of trabeculae of SI and ML directions.

DEM의 정확도 분석에 의한 도시 소유역의 유출해석

박진형,이관수,이삼노 한국지리정보학회 2004 한국지리정보학회지 Vol.7 No.1

본 연구는 수치지형자료의 해상도와 보간법의 변화에 따른 정확도 분석을 실시하여 도시소유역의 유출현상을 해석하고자 하였다. 수치자료는 격자크기별로 TIN 보간법을 이용하여 DEM 자료를 생성하고, 정확도 분석은 수치지도의 등고선을 이용하여 결정계수(R2)와 회귀식을 도출하였다. 검증된 DEM 자료를 사용하여 소유역구분, 면적, 유역폭, 지면경사도 등의 지형인자를 추출하여 도시유출모형에 적용시켜 여서-문수지구의 도시 소유역에 가장 적합한 유출모형을 알아보고자 한다. 모형의 적용 결과 SWMM의 유출수문곡선이 ILLUDAS의 유출수문곡선보다 실측값에 더 근사하게 나타났고, SWMM의 경우 실측값과 최대 19%, 최소 5%, 평균 13%의 오차를 나타냈다. 본 연구대상지역과 같은 도시소유역은 강우지속시간이 첨두도달시간에 미치는 영향은 미미하게 나타났다. 본 연구의 결과로 SWMM 모형이 여서-문수지역의 도시유출모형으로 적합하며 적용면에서도 더 다양한 기능과 정확성을 나타낸다고 판단된다. The purpose of this study is to analyze the urban hydrologic state by the use of GIS, resolution and interpolation. The determination coefficient(R2) and Regression Formula were derived from the contour of digital map for the accuracy, and DEM data was made by using TIN interpolation by the size of the grid. By using the observed DEM data, topographical factors were extracted from the small basin, size, the width of a basin and the slope, and were applied in the urban runoff model. Through the model, we tried to find out the most suitable runoff model in a small basin of Yosu-Munsu area. As a result of applying models to the drainage considered, the runoff hydrograph estimated by SWMM model was closer to the observed one than that estimated by ILLUDAS model. The difference between the runoff hydrograph by SWMM and the observed one is maximum error of 19%, minimum error of 5% and average error of 13%. The influence of duration in contrast to pick time is insignificant in a urban small basin. As a conclusion of this study, SWMM model was more suitable and applicable for the urban runoff model than ILLUDAS model due to its accuracy and various abilities.

하악에 발생한 양성골아세포종의 증례보고

박태원,이삼선,유동수,최순철 大韓口腔顎顔面 放射線學會 1993 Imaging Science in Dentistry Vol.23 No.1

임플랜트를 위한 하악골 측정시 전산화단층사진상의 정확도에 관한 연구: 하악 위치와 gantry각이 미치는 영향

박태원,유동수,최항문,이삼선,박래정,최순철 大韓口腔顎顔面 放射線學會 1998 Imaging Science in Dentistry Vol.28 No.1

We used five adult dog mandibles embedded in resin block and six different cross-sectional planes for each mandible were choosen. According to the angle of mandibular occulsal plane to vertical plane(mandibular angle)and gantry angle of CT machine, we classified 4 experimental groups and 1 control group. The control group images were taken at the mandibular angel 0˚and gantry angle 0˚. The experimental images were taken at the mandibular angle 15˚and gantry angle 0˚(group 1);30˚and 0˚(group 2);15˚and 15˚(group 3);30˚and 30˚(group 4), respectively, Using the reformatted cross-sectional images, the distance from the mandibular canal to the alveolar crest and the distance from the mandibular canal to the buccal cortex and to the lingual cortex was measured and compared. The obtained results were as follows; 1. The distance from the mandibular canal to the alveolar crest of group 1 and 2 was larger than control group, but the distance of group 3 and 4 was smaller. The distance from the mandibular canal to the buccal cortex and to the lingual cortex of all experimental groups was smaller than control group. 2. The distance from the mandibular canal to the alveolar crest showed the largest difference from control group in all experimental groups, especially in group 2 and 4(p<0.05)> 3. In the distance from the mandibular canal to the alveolar crest, the number of deviation value under 1mm was 20 in group 3 and was 11 in group 2 and 4, respectively. 4. The deviation value of the distance from the mandibular canal to the buccal cortex and to the lingual cortex was under 1 mm in most cases.

냉동 제대혈 세포의 체외 증폭

김삼용,김철희,배광봉,김현수,박상준,김종숙,윤환중,조덕연 충남대학교 암연구소 1998 癌共同硏究所 硏究誌 Vol.2 No.1

Background : Cord blood(CB), which has no HLA restriction, is an alternative to bone marrow for hematopoietic stem cell transplantation. The use of cord blood, however, is limited by the number of progenitor/stem cells necessary to reconstitute the older child or adult. Therefore, ex vivo expansion of CB could have tremendous impact on diverse clinical settings. We studied the ex vivo expansion of isolated population of CD34_(+) cells from cryopreserved CB cells. Methods : CD34 cells were isolated from cryopreserved CB mononuclear cells. Purified cells were cultured with various combinations of hematopoietic growth factors including erythropoietin(EPO), stem cell factor(SCF), granulocyte-colony-stimulating factor(G-CSF), gra-nulocyte, macrophage-colony-stimulating factor(GM-CSF), interleukin-1β(IL-1β), 1L-3, and IL-6. After 7, 10 or 14 days of culture, the fold increases of colony-forming unit- granu-locyte, macrophage(CFU-GM), burst-forming unit-erythroid(BFU-E), colony-forming unit-mix (CFU-Mix), and high proliferative potential colony-forming cell(HPP-CFC) were evaluated. Results : Ten-day culture with the combination of EPO, SCF, G-CSF, IL-1β, and IL-3 resulted in a median of 60-fold increase of CFU-GM, which was greater than those with the combinations of less than 5 growth factors. The addition of IL-6 or GM-CSF to this combination did not enhance CFU-GM expansion. Ten-day culture was significantly superior to 7-day culture for CFU-GM expansion. Prolongation of culture to 14 days, however, revealed decreased expansion of CFU-GM compared to 10 days. BFU-E and CFU-Mix were expanded to 2~5 folds in 7-day culture with the combination of EPO, SCF, and G-CSF. Further expansion was not achieved in 10-day culture and colonies disappeared in 14-day culture. HPP-CFC was expanded to a median of 7.5 folds in 7-day culture with the combination of EPO, SCF, G-CSF, IL-1β, IL-3, and IL-6. Neither 10-day or 14 day-culture enhanced expansion of HPP-CFU. Conclusion : Cryopreserved cord blood cells maintain ex vivo expansion potential. In our system, 10-day culture with the combination consisting of EPO, SCF, G-CSF, IL-1β, and IL-3 seems to be adequate for hematopoietic progenitor/stem cell expansion from cryopreserved cord blood cells.

저기압 방전 플라즈마내 전자비임의 특성

河三洙,朴德圭 경북대학교 과학교육연구소 1988 科學敎育硏究誌 Vol.12 No.-

The electron gun which is placed at low pressure air discharge tube produces electron beam. The electron densities, the electron profiles and the energy distributions of the beam are measured by a single probe. As the result of these measurements, the following characteristics of the lectron beam are obtained. In a steady state, the magnetic field intensity of 6.3 KAT and the pressure of 40 mtorr, as discharge current is increased from 16mA to 20mA, the electron density at the center of z-axis varied from 8.8 x 10^9 cm^-3 to 12.2 x 10^9 cm ^-3 and FWHM varied a little from 1.30cm to 0.40cm. In a steady state, the discharge current of 20mA and the pressure of 40 mtorr, as the magnetic field intensity is increased from 2.7 KAT to 6.3 KAT, the electron density varied from 10.1 x 10^9 cm^-3 to 12.2 x 10^9 cm^-3 and FWHM decreased from 1.6 cm to 0.8 cm. In the steady state, the discharge current of 20mA, the magnetic field intensity of 6.3KAT and the pressure of 40 mtorr, as the distance of probe from cathode is increased from 40cm to 80cm, the electron density decreased from 12.2 x 10^9 cm ^-3 to 12.2 x 10^9 cm ^-3 and FWHM decreased from 1.6 cm to 0.8 cm. In the steady state, the discharge current of 20mA, the magnetic field intensity of 6.3KAT and the pressure of 40 mtorr, as the distance of probe from cathode is increased from 40cm toe 80cm, the electron density decreased from 12.2 x 10^9^-3 to 7.9 x 10^9 cm ^-3 and FWHM decreased a little from 0.40 cm to 0.28 cm. It is also measured that the high energy electrons are mostly distributed between center of the axis and beam surface (r = 0.2cm).

방사선조사 후 유표피암종세포내 칼슘농도의 변화와 apoptosis 발현에 관한 연구

이삼선,문제운,허민석,박태원,유동수,최순철 大韓口腔顎顔面 放射線學會 1999 Imaging Science in Dentistry Vol.29 No.1

Purpose: Ionizing radiations have been reported as an apoptosis initiating stimulus in various cells and it has established that sustained elevations in [Ca²+] can lead to DNA fragmentation by Ca²+-dependent endonucleases, ultimately resulting in apoptotic cell death. The previous experiments have been reported by using primarily thymocytes and lymphocytes and the change of [Ca²+] was measured only by minutes or hours respectively. We need to evaluate [Ca²+] in both several minutes and hours after irradiation of radiation of radiation therapy and verify the apoptotic cells. Materials and Methods: We have measured [Ca²+] in human gingival epitheloid cancer cell with 10 Gy irradiation, at minutely intervals and hourly intervals using digitized video-intensified fluorescence microscopy and the fluorescent Ca²+ indicator dye, fura-2. In order to find out that the transient rise in [Ca²+] could induced apoptosis, cells were incubated for 1 hour at 37℃ with TdT enzyme, rinsed and resuspended containing fluorescence and observed under a confocal fluorescence microscope. MTT assay was done to determine cell activity and LDH assay was done to determine the amount of necrotic cells. Results: After irradiation, the transient and temporal increasing of [Ca²+] in the KB cells was founded. Though, there was no change in the intracellular [Ca²+] at 30 minutes and 2 hours after irradiation. We could detect of DNA fragmented cells at 4 hours after 10 Gy irradiated cells. There were no significant differences between 4 hour, 1 day, 3 day cells. There were no significant differences in MTT and LDH assay between the irradiated group and the control group after 4 hours and 1 day. Though after 3 days there were differences in MTT and LDH assay between the irradiated group was significantly decreased than the control group, in LDH assay the number of necrotic cell death of the irradiated was higher than the control group. Conclusion: In KB cells there were incipient and temporal increasing of the [Ca²+] with 10 Gy irradiation and the apoptosis was founded from 4 hours later which was earlier than seeing of the change of the amount of the cellular ability and necrosis.(J Korean Oral Maxillofac Radiol 1999:29:109-117)

인체의 암세포주와 치은섬유모세포주에서 방사선조사가 apoptosis 유발에 미치는 영향에 관한 연구

유동수,최순철,이삼선,박무순,박태원 大韓口腔顎顔面 放射線學會 1998 Imaging Science in Dentistry Vol.28 No.1

The radiation-induced apoptosis was studied for two human cancer cell lines (KB cells, RPMI 2650 cells) and the human gingival fibroblast cell line (HGF-1 cells). The single irradiation of 2, 10, 20Gy was done with 241.5 cGy/min dose rate using the ?? MK cell irradiator. The cells were stained with propidium iodide and examined under the fluoro-microscope and assayed with the flow cytometry a day after irradiation. Also, the LDH assay was done to determine the amount of necrotic cells. The obtained results were as follows: 1. On the fluoro-microscope, many fragmented nuclei were detected in the KB, RPMI 2650, and HGF-1 cells after irradiation. 2. On the DNA content histogram obtained from the flow cytometry, the percentages of the pre-Gi peak of the control and 2,10 and 20Gy irradiation group were 4.5, 55.0, 52.3, and 66.6% on KB cells, 2.7, 3.3, 31.8, and 32.6% on RPMI 2650 cells and 2.8, 21.8, 30.4, and 40.2% on HGF-1 cells respectively. 3. The number of G1-stage cells was abruptly decreased after 2Gy irradiation on KB cells and 10Gy irradiation on RPMI 2650 cells, But there was a slight decrease without regard to irradiation dose on HGF-1 cells. 4. There was no significantly different absorbance in extracellular LDH assay along the experimental cell lines.