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Implantation of Cultured Preadipocyte Using Chitosan/Alginate Sponge
Rhie, Jong Won,Song, Jin Kyung,Lee, Paik Kwon,Ahn, Sang Tae Trans Tech Publications, Ltd. 2007 Key Engineering Materials Vol.342-343 No.-
<P>Alginate was a proven biocompatible biomatrice for cells but it was known not to provide a proper microenvironment needed for the proliferation of cells because of its anionic property, which caused its low affinity for cells. Water-soluble chitosan was well known as wound healing material and it also had cationic property which helped cell-to-matrix adhesion. The purpose of this study is to assess the ability of a chitosan/alginate mixed sponge as a scaffold for preadipocytes to serve as a biological implant for soft tissue augmentation. Chitosan/alginate and calcium alginate sponges were made by lyophilizing of alginate with water-soluble chitosan mixture and with calcium chloride mixture, respectively, and those were observed by SEM. Preadipocytes seeded in those sponges were cultured for 2 weeks. In vivo study was designed that chitosan/alginate sponges with and without preadipocytes were implanted subcutaneously into nude mouse. Chitosan/alginate and calcium alginate sponges which had highly porosity and 50-200㎛ pore size. In the chitosan/alginate sponge, the levels of DNA amount were significantly higher than those in calcium alginate sponge (P<0.05). In both groups, they increased progressively with time. On the in vivo study, it was observed that adipose tissue layer in the margin of chitosan/alginate sponge on the 2 weeks after implantation of nude mouse. On the 8 weeks after implantation, thick layer of adipose tissue and neovascularization were observed in the chitosan/alginate sponge. Consequently, chitosan/alginate sponge provided proper microenvironment to human preadipocyte, increased the cell proliferation and maintained the pore that offered neovascularization, so turned out to be effective form of fat transplantation for soft tissue augmentation and reconstruction.</P>
Rhie, Jong Won,Cho, Hyun Mi,Lee, Hee Young,Han, Dong Keun Trans Tech Publications, Ltd. 2007 Key Engineering Materials Vol.342-343 No.-
<P>Human adipose tissue contain a population of pluripotent stem cells capable of differentiating along multiple mesenchymal cell lineages. The goal of this study was to examine the chondrogenic potential of adipose-derived stem (ADS) cells. ADS cells were isolated from human subcutaneous adipose tissue obtained by lipectomy and liposuction, and were expanded and grown in vitro with or without chondrogenic medium in micromass culture condition and 3D culture condition in PLGA(poly(lactic-co-glycolic acid)) scaffold. Human ADS cells of micromass culture condition and 3D culture condition in PLGA scaffolds, were differentiated with chondrogenic medium consisted of transforming growth factor-β1, insulin-transferrin-selenium, dexamethasone and ascorbate-2-phosphate. ADS cells abundantly synthesized cartilage matrix molecules including collagen type II, VI and link protein. ADS cells under high-density micromass culture condition with chondrogenic medium formed well defined nodules within 48 hours of induction. On the 3rd week after chondrogenic differentiation of ADS cells under the micromass culture condition and 3D culture condition of PLGA scaffold, mRNA of type II collagen, type VI collagen and link protein were expressed by reverse transcription polymerase chain reaction (RT-PCR). On the 3weeks, content of glycosaminoglycan in cells treated with chondrogenic medium was greater than that with non chondrogenic medium(control).On the 3rd week culture under the chondrogenic medium, hematoxylin & eosin (H & E) staining, alcian blue staining and type II collagen immunohistochemistry analysis confirmed the chondrogenic differentiation in micromass and 3D cultured specimen. These findings document the ability of ADS cells to produce characteristic cartilage matrix molecules, and provide the possibility of cartilage regeneration for cartilage substitution.</P>
A Pilot Study of Skin Resurfacing Using the 2,790-nm Erbium:YSGG Laser System
Rhie, Jong Won,Shim, Jeong Su,Choi, Won Seok Korean Society of Plastic and Reconstructive Surge 2015 Archives of Plastic Surgery Vol.42 No.1
Background The erbium:yttrium scandium gallium garnet (Er:YSGG) laser differs from other laser techniques by having a faster and higher cure rate. Since the Er:YSGG laser causes an appropriate proportion of ablation and coagulation, it has advantages over the conventional carbon dioxide ($CO_2$) laser and the erbium-doped yttrium aluminum garnet (Er:YAG) laser, including heating tendencies and explosive vaporization. This research was conducted to explore the effects and safety of the Er:YSGG laser. Methods Twenty patients participated in the pilot study of a resurfacing system using a 2,790-nm Er:YSGG laser. All patients received facial treatment by the 2,790-nm Er:YSGG laser system (Cutera) twice with a 4-week interval. Wrinkle reduction, reduction in pigment inhomogeneity, and improvement in tone and texture were measured. Results Study subjects included 15 women and five men. Re-epithelization occurred in all subjects 3 to 4 days after treatment, and wrinkle reduction, reduction in pigment inhomogeneity, and improvement in tone and texture within 6 months of treatment. Conclusions The 2,790-nm YSGG laser technique had fewer complications and was effective in the improvement of scars, pores, wrinkles, and skin tone and color with one or two treatments. We expect this method to be effective for people with acne scars, pore scars, deep wrinkles, and uneven skin texture and color.
자유 형상 제작 방식의 다축 적층 시스템 개발 및 쥐 모델을 이용한 PCL/PLGA/TCP 인공지지체의 골 형성 능력 평가
김종영(Jong Young Kim),심진형(Jin-Hyung Shim),김상헌(Sang-Heon Kim),이종원(Jong-Won Rhie),조동우(Dong-Woo Cho) 대한기계학회 2010 대한기계학회 춘추학술대회 Vol.2010 No.5
We investigated the validity of using SFF(solid free-form fabrication)-based scaffolds seeded with osteoblasts, and human umbilical vein endothelial cells (HUVECs) to enhance bone regeneration capacity. To accomplish this goal, SFF-based polycaprolactone (PCL)/poly-lactic-co-glycolic acid (PLGA)/tri-calcium phosphate (TCP) scaffolds were fabricated using a multi-head deposition system (MHDS). The blended PCL/PLGA/TCP scaffolds were seeded with osteoblasts and HUVECs and implanted into calvaria defect model in rats. At 8 and 12 weeks after implantation, micro-computed tomography (μ-CT), reverse transcription polymerase chain reaction (RT-PCR), and histological assays were conducted to know the effects of SFF-based scaffolds on osteogenesis. In vivo results indicated scaffolds in the osteoblast-HUVEC group had the largest area of new bone tissue. Therefore, we demonstrated through μ-CT and histological assays that scaffolds seeded with both human osteoblasts and HUVECs were superior to other groups.
The Development of Phasic and Tonic Inhibition in the Rat Visual Cortex
Hyun-Jong Jang,Kwang-Hyun Cho,Sung-Won Park,Myung-Jun Kim,Shin Hee Yoon,Duck-Joo Rhie 대한생리학회-대한약리학회 2010 The Korean Journal of Physiology & Pharmacology Vol.14 No.6
Gamma-aminobutyric acid (GABA)-ergic inhibition is important in the function of the visual cortex. In a previous study, we reported a developmental increase in GABA<sub>A</sub> receptor-mediated inhibition in the rat visual cortex from 3 to 5 weeks of age. Because this developmental increase is crucial to the regulation of the induction of long-term synaptic plasticity, in the present study we investigated in detail the postnatal development of phasic and tonic inhibition. The amplitude of phasic inhibition evoked by electrical stimulation increased during development from 3 to 8 weeks of age, and the peak time and decay kinetics of inhibitory postsynaptic potential (IPSP) and current (IPSC) slowed progressively. Since the membrane time constant decreased during this period, passive membrane properties might not be involved in the kinetic changes of IPSP and IPSC. Tonic inhibition, another mode of GABA<sub>A</sub> receptor-mediated inhibition, also increased developmentally and reached a plateau at 5 weeks of age. These results indicate that the time course of the postnatal development of GABAergic inhibition matched well that of the functional maturation of the visual cortex. Thus, the present study provides significant insight into the roles of inhibitory development in the functional maturation of the visual cortical circuits.
Effects of Serotonin on the Induction of Long-term Depression in the Rat Visual Cortex
Hyun-Jong Jang,Kwang-Hyun Cho,Sung-Won Park,Myung-Jun Kim,Shin Hee Yoon,Duck-Joo Rhie 대한생리학회-대한약리학회 2010 The Korean Journal of Physiology & Pharmacology Vol.14 No.5
Long-term potentiation (LTP) and long-term depression (LTD) have both been studied as mechanisms of ocular dominance plasticity in the rat visual cortex. In a previous study, we suggested that a developmental increase in serotonin [5-hydroxytryptamine (5-HT)] might be involved in the decline of LTP, since 5-HT inhibited its induction. In the present study, to further understand the role of 5-HT in a developmental decrease in plasticity, we investigated the effect of 5-HT on the induction of LTD in the pathway from layer 4 to layer 2/3. LTD was inhibited by 5-HT (10ՌM) in 5-week-old rats. The inhibitory effect was mediated by activation of 5-HT<sub>2</sub> receptors. Since 5-HT also regulates the development of visual cortical circuits, we also investigated the role of 5-HT on the development of inhibition. The development of inhibition was retarded by chronic (2 weeks) depletion of endogenous 5-HT in 5-week-old rats, in which LTD was reinstated. These results suggest that 5-HT regulates the induction of LTD directly via activation of 5-HT<sub>2</sub> receptors and indirectly by regulating cortical development. Thus, the present study provides significant insight into the roles of 5-HT on the development of visual cortical circuits and on the age-dependent decline of long-term synaptic plasticity.