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        소의 동결수정란이 수태에 미치는 영향 1 . 그리세롤부유액에 의한 육단계평형의 영향

        석호봉,이광원,신용식,김호중,조윤연,지설하,오대균,임경순,알피 엘스덴 ( H . B . Seok,K . W . Lee,Y . S . Shin,H . J . Kim,y . y . Cho,S . H . Chee,D . K . Oh,K . S . Im,R . P . Elsden ) 한국축산학회 1983 한국축산학회지 Vol.25 No.4

        Nineteen frozen embryos with high genetic-potency were imported from Holstein Registration Association of America and transferred to recipients of the National Livestock Breeding Station. The synchronization was induced by twice intramuscular injections of 25㎎ PGF₂α with 14 days interval. The frozen embryos thawed at 37℃ were rehydrated within 6 steps in order to remove glycerol of the medium. The embryos were transferred to 19 recipients with non-surgical method and the results were as follows. 1. On 1st and 2nd intramuscular injection of PGF₂α, twentyeight (68.3%) and thirty-six (87.8%) among 41 treated showed estrus within 4 days after treatment. 2. The rate of the standing heat on day 3 after injection of PGF₂α was 53.7 and 73.6% at 1st arid 2nd injection, respectively. 3. The rate of the heifers showing standing heat after 1st and 2nd injection was 79.2 and 91.7%, but that of the cows was 52.9 and 82.4%. The rate was higher in heifer than cow. The rate of heifer selected for embryo transfer among synchronized recipients was also higher (62.5%) than that of cow (23.5%). 4 The evaluation of 19 frozen embryos (12 Holsteins and 7 Limousins) exception of 4 eggs with broken zona pellucida was generally good quality blastocysts as pre-freezing embryos. The pregnancy was obtained only from blastocyst. 5. At the examination of pregnancy at 60 days after nonsurgical transfers, three (15.8%) of 19 recipients were concepted. As the results, 2 female Holsteins (16.7%) and 1 male Limousin (14.3%) were delivered in terms of normal pregnant period.

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