Achromobacter panacis sp. nov., isolated from rhizosphere of Panax ginseng

Priyanka Singh,김연주,Hina Singh,Mohamed El-Agamy Farh,양덕춘 한국미생물학회 2017 The journal of microbiology Vol.55 No.6

A novel strain DCY105T was isolated from soil collected from the rhizosphere of ginseng (Panax ginseng), in Gochang, Republic of Korea. Strain DCY105T is Gram-reaction-negative, white, non-motile, non-flagellate, rod-shaped and aerobic. The bacteria grow optimally at 30°C, pH 6.5–7.0 and in the absence of NaCl. Phylogenetically, strain DCY105T is most closely related to Achromobacter marplatensis LMG 26219T (96.81%). The DNA G+C content of strain DCY105T was 64.4 mol%. Ubiquinone 8 was the major respiratory quinone, and phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol were amongst the major polar lipids. C16:00, C8:03OH and iso-C17:03OH were identified as the major fatty acids present in DCY105T. The results of physiological and biochemical tests allowed strain DCY105T to be differentiated phenotypically from other recognized species belonging to the genus Achromobacter. Therefore, it is suggested that the newly isolated organism represents a novel species, for which the name Achromobacter panacis sp. nov. is proposed with the type strain designated as DCY105T (=CCTCCAB 2015193T =KCTC 42751T).

Sphingomonas panacis sp. nov., isolated from rhizosphere of rusty ginseng

Singh, Priyanka,Kim, Yeon-Ju,Hoang, Van-An,Farh, Mohamed El-Agamy,Yang, Deok-Chun Springer-Verlag 2015 Antonie van Leeuwenhoek Vol.108 No.3

Primary pulmonary epithelioid inflammatory myofibroblastic sarcoma: a rare entity and a literature review

Priyanka Singh,Aruna Nambirajan,Manish Kumar Gaur,Rahul Raj,Sunil Kumar,Prabhat Singh Malik,Deepali Jain 대한병리학회 2022 Journal of Pathology and Translational Medicine Vol.56 No.4

Epithelioid inflammatory myofibroblastic sarcoma (EIMS) is an aggressive subtype of inflammatory myofibroblastic tumor (IMT) harboring anaplastic lymphoma kinase (<i>ALK</i>) gene fusions and is associated with high risk of local recurrence and poor prognosis. Herein, we present a young, non-smoking male who presented with complaints of cough and dyspnoea and was found to harbor a large right lower lobe lung mass. Biopsy showed a high-grade epithelioid to rhabdoid tumor with ALK and desmin protein expression. The patient initially received 5 cycles of crizotinib and remained stable for 1 year; however, he then developed multiple bony metastases, for which complete surgical resection was performed. Histopathology confirmed the diagnosis of EIMS, with <i>ALK</i> gene rearrangement demonstrated by fluorescence in situ hybridization. Postoperatively, the patient is asymptomatic with stable metastatic disease on crizotinib and has been started on palliative radiotherapy. EIMS is a very rare subtype of IMT that needs to be included in the differential diagnosis of ALKexpressing lung malignancies in young adults.

Cupriavidus yeoncheonense sp. nov., isolated from soil of ginseng.

Singh, Priyanka,Kim, Yeon-Ju,Nguyen, Ngoc-Lan,Hoang, Van-An,Sukweenadhi, Johan,Farh, Mohamed El-Agamy,Yang, Deok-Chun N.V. Swets en Zeitlinger 2015 Antonie van Leeuwenhoek Vol.107 No.3

<P>A novel bacterial strain, DCY86(T) (=KCTC 42053(T)?=?JCM 19890(T)) was isolated from soil of a ginseng field in Yeoncheon province (3804'00N 12657'00E), Republic of Korea using a serial dilution method. Strain DCY86(T) was observed to be Gram-stain negative, strictly aerobic, to grow optimally at 25-30?C, at pH 7-7.5 and on tryptic soya agar medium. The cells were found to be sensitive to ceftazidine and tetracycline. Based on 16S rRNA gene sequence comparisons, strain DCY86(T) was found to be most closely related to Cupriavidus basilensis LMG 18990(T) (98.48?%), Cupriavidus numazensis LMG 26411(T) (98.34?%), Cupriavidus pinatabonesis KCTC 22125(T) (98.34?%) and Cupriavidus laharis KCTC 22126(T) (98.00?%). The G+C content was determined to be 64.23?mol?%. The only isoprenoid quinone detected in strain DCY86(T) was ubiquinone Q-8. The major polar lipids were identified as diphosphatidylglycerol, phosphtidylethanolamine, phosphatidylglycerol, unidentified aminophosphoglycolipids and unidentified phospholipids. The major fatty acids were identified as C16:0 summed feature 3 (C16:1 ω7c/ω6c and/or iso-C15?:?0 2-OH) and summed feature 8 (C18:1 ω7c and/or C18:1 ω6c). These data support the affiliation of strain DCY86(T) to the genus Cupriavidus. Strain DCY86(T) was also found to be able to solubilize phosphate and produce siderophores. The results of physiological and biochemical tests enabled strain DCY86(T) to be differentiated genotypically and phenotypically from the recognized species of the genus Cupriaividus. Therefore, the novel isolate can be considered to represent a novel species, for which the name Cupriavidus yeoncheonense sp. nov. is proposed here. The type strain is DCY86(T) (=KCTC 42053(T)?=?JCM 19890(T)).</P>

Engineering of mesoporous silica nanoparticles for release of ginsenoside CK and Rh2 to enhance their anticancer and anti-inflammatory efficacy: in vitro studies

Singh, Priyanka,Singh, Hina,Castro-Aceituno, Veró,nica,Ahn, Sungeun,Kim, Yeon Ju,Farh, Mohamed El-Agamy,Yang, Deok Chun KLUWER 2017 JOURNAL OF NANOPARTICLE RESEARCH Vol.19 No.7

<P>The current study highlights the fabrication of drug delivery system by utilizing 200 nm mesoporous silica nanoparticles (MSNPs) with 4-nm pore size, as a carrier system for delivery ginsenoside compound K (CK) and Rh2 to enhance their efficacy. The two pharmacologically imperative ginsenosides, CK and Rh2, were loaded to the MSNPs to prepare MSNPs-CK and MSNPs-Rh2, respectively. A fluorescein isothiocyanate (FITC) fluorescent dye was combined in the MSNPs carrier system, in order to trace the cellular uptake of ginsenoside-loaded nanoparticles for in vitro studies. Following purification, the so-prepared MSNPs-CK-FITC and MSNPs-Rh2-FITC were characterized by several analytical techniques, which includes, high-pressure liquid chromatography (HPLC), H-1 NMR, field emission transmission electron microscopy (FE-TEM), Fourier transform infrared spectroscopy (FT-IR), x-ray diffraction (XRD), thermogravimetric analysis (TGA), and dynamic light scattering (DLS). In vitro cytotoxicity assay in HaCaT skin cells, A549 lung cancer cells, HepG2 liver carcinoma cells, and HT-29 colon cancer cell lines were tested for MSNPs-CK-FITC and MSNPs-Rh2-FITC. The results demonstrate the excellent biocompatibility of nanoparticles in normal cell lines (HaCaT skin cells) and anticancer efficacy in all the tested cancer cell lines at 10-mu M concentration. Additionally, the in vitro anti-inflammatory behavior of MSNPs-CK-FITC and MSNPs-Rh2-FITC were checked in RAW264.7 (murine macrophage) cell lines. The outcomes showed higher anti-inflammatory efficacy of MSNPs-CK-FITC and MSNPs-Rh2-FITC as compared to standard ginsenosides CK and Rh2 in RAW264.7 cell lines. Thus, with 200 nm MSNPs carrier system for the delivery ginsenosides CK and Rh2, a high amount of loading and increasing in vitro pharmacological efficacies of ginsenosides were realized. This study may provide useful insights for designing and improving the applicability of MSNPs for ginsenoside delivery.</P>

Biosynthesis, characterization, and antimicrobial applications of silver nanoparticles

Singh, Priyanka,Kim, Yeon Ju,Singh, Hina,Wang, Chao,Hwang, Kyu Hyon,Farh, Mohamed El-Agamy,Yang, Deok Chun Dove Medical Press 2015 INTERNATIONAL JOURNAL OF NANOMEDICINE Vol.10 No.-

<P>In the present study, the strain <I>Brevibacterium frigoritolerans</I> DC2 was explored for the efficient and extracellular synthesis of silver nanoparticles. These biosynthesized silver nanoparticles were characterized by ultraviolet-visible spectrophotometry, which detected the formation of silver nanoparticles in the reaction mixture and showed a maximum absorbance at 420 nm. In addition, field emission transmission electron microscopy revealed the spherical shape of the nanoparticles. The dynamic light scattering results indicated the average particle size of the product was 97 nm with a 0.191 polydispersity index. Furthermore, the product was analyzed by energy dispersive X-ray spectroscopy, X-ray diffraction, and elemental mapping, which displayed the presence of elemental silver in the product. Moreover, on a medical platform, the product was checked against pathogenic microorganisms including <I>Vibrio parahaemolyticus</I>, <I>Salmonella enterica</I>, <I>Bacillus anthracis</I>, <I>Bacillus cereus</I>, <I>Escherichia coli</I>, and <I>Candida albicans</I>. The nanoparticles demonstrated antimicrobial activity against all of these pathogenic microorganisms. Additionally, the silver nanoparticles were evaluated for their combined effects with the commercial antibiotics lincomycin, oleandomycin, vancomycin, novobiocin, penicillin G, and rifampicin against these pathogenic microorganisms. These results indicated that the combination of antibiotics with biosynthesized silver nanoparticles enhanced the antimicrobial effects of antibiotics. Therefore, the current study is a demonstration of an efficient biological synthesis of silver nanoparticles by <I>B. frigoritolerans</I> DC2 and its effect on the enhancement of the antmicrobial efficacy of well-known commercial antibiotics.</P>

Biological Synthesis of Nanoparticles from Plants and Microorganisms

Singh, Priyanka,Kim, Yu-Jin,Zhang, Dabing,Yang, Deok-Chun Elsevier 2016 Trends in biotechnology Vol.34 No.7

<P>Nanotechnology has become one of the most promising technologies applied in all areas of science. Metal nanoparticles produced by nanotechnology have received global attention due to their extensive applications in the biomedical and physiochemical fields. Recently, synthesizing metal nanoparticles using microorganisms and plants has been extensively studied and has been recognized as a green and efficient way for further exploiting microorganisms as convenient nanofactories. Here, we explore and detail the potential uses of various biological sources for nanoparticle synthesis and the application of those nanoparticles. Furthermore, we highlight recent milestones achieved for the biogenic synthesis of nanoparticles by controlling critical parameters, including the choice of biological source, incubation period, pH, and temperature.</P> <P><B>Trends</B></P> <P>The biological synthesis of nanoparticles is increasingly regarded as a rapid, ecofriendly, and easily scaled-up technology.</P> <P>Metal nanoparticles produced using microorganisms and plant extracts are stable and can be monodispersed by controlling synthetic parameters, such as pH, temperature, incubation period, and mixing ratio.</P> <P>Recently, biological nanoparticles were found to be more pharmacologically active than physicochemically synthesized nanoparticles.</P> <P>Among the various biological nanoparticles, those produced by medicinal plants have been found to be the most pharmacologically active, possibly due to the attachment of several pharmacologically active residues.</P>

In situ preparation of water-soluble ginsenoside Rh2-entrapped bovine serum albumin nanoparticles: in vitro cytocompatibility studies

Singh, Priyanka,Kim, Yeon Ju,Singh, Hina,Ahn, Sungeun,Castro-Aceituno, Veró,nica,Yang, Deok Chun DOVE MEDICAL PRESS 2017 INTERNATIONAL JOURNAL OF NANOMEDICINE Vol.12 No.-

<P>The present study investigates a simple and convenient one-step procedure for the preparation of bovine serum albumin (BSA)-Rh2 nanoparticles (NPs) at room temperature. In this work, ginsenoside Rh2 was entrapped within the BSA protein to form BSA-Rh2 NPs to enhance the aqueous solubility, stability, and therapeutic efficacy of Rh2. The physiochemical characterization by high-performance liquid chromatography, nuclear magnetic resonance, Fourier transform infrared spectroscopy, field emission transmission electron microscopy, dynamic light scattering, and thermogravimetric analysis confirmed that the prepared BSA-Rh2 NPs were spherical, highly monodispersed, and stable in aqueous systems. In addition, the stability of NPs in terms of different time intervals, pHs, and temperatures (20°C–700°C) was analyzed. The results obtained with different pHs showed that the synthesized BSA-Rh2 NPs were stable in the physiological buffer (pH 7.4) for up to 8 days, but degraded under acidic conditions (pH 5.0) representing the pH inside tumor cells. Furthermore, comparative analysis of the water solubility of BSA-Rh2 NPs and standard Rh2 showed that the BSA nanocarrier enhanced the water solubility of Rh2. Moreover, in vitro cytotoxicity assays including cell viability assays and morphological analyses revealed that Rh2-entrapped BSA NPs, unlike the free Rh2, demonstrated better in vitro cell viability in HaCaT skin cell lines and that BSA enhanced the anticancer effect of Rh2 in A549 lung cell and HT29 colon cancer cell lines. Additionally, anti-inflammatory assay of BSA-Rh2 NPs and standard Rh2 performed using RAW264.7 cells revealed decreased lipopolysaccharide-induced nitric oxide production by BSA-Rh2 NPs. Collectively, the present study suggests that BSA can significantly enhance the therapeutic behavior of Rh2 by improving its solubility and stability in aqueous systems, and hence, BSA-Rh2 NPs may potentially be used as a ginsenoside delivery vehicle in cancer and inflammatory cell lines.</P>

Ecofriendly synthesis of silver and gold nanoparticles by <i>Euphrasia officinalis</i> leaf extract and its biomedical applications

Singh, Hina,Du, Juan,Singh, Priyanka,Yi, Tae Hoo Informa UK (Taylor Francis) 2018 Artificial cells, nanomedicine, and biotechnology Vol.46 No.6

A Class of Estimators for Population Variance in Two Occasion Rotation Patterns

Singh, G.N.,Priyanka, Priyanka,Prasad, Shakti,Singh, Sarjinder,Kim, Jong-Min The Korean Statistical Society 2013 Communications for statistical applications and me Vol.20 No.4

A variety of practical problems can be addressed in the framework of rotation (successive) sampling. The present work presents a sample rotation pattern where sampling units are drawn on two successive occasions. The problem of estimation of population variance on current (second) occasion in two - occasion successive (rotation) sampling has been considered. A class of estimators has been proposed for population variance that includes many estimators as a particular case. Asymptotic properties of the proposed class of estimators are discussed. The proposed class of estimators is compared with the sample variance estimator when there is no matching from the previous occasion. Optimum replacement policy is discussed. Results are supported with the empirical means of comparison.