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RISS 인기검색어
- 검색키워드
- 저자 : Ningthoujam Sandhyarani (검색결과 3 건)
- 무료
- 기관 내 무료
- 유료
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Clonal Propagation of Triploid Acorus calamus Linn. Using Dual-Phase Culture System
Sandhyarani, Ningthoujam,Kishor, Rajkumar,Sharma, Gurumayum Jitendra 한국작물학회 2011 Journal of crop science and biotechnology Vol.14 No.3
Acorus calamus is an important medicinal plant which has been used in Indian traditional medicine since time immemorial. Various bioactive molecules, viz., acorin, ${\alpha}$- and ${\beta}$-asarone, asaryldehyde, caryophylene, isoasarone, methylisoeugenol, and safrol have been isolated from this plant. However, the use of this plant for medicinal purpose has been recently banned due to the high toxic property of ${\beta}$-asarone. The triploid Acorus calamus is reported to be low in ${\beta}$-asarone content and thus found to be the ideal raw material for medicinal use. The present investigation represents our finding for successful in vitro clonal propagation of the elite triploid accessions of Acorus calamus for mass propagation. In the dual-phase culture system consisting of agar-solidified Murashige and Skoog medium overlaid by liquid fraction of the same medium, maximum multiple shoot induction was favored by supplementation of ${\alpha}$-naphthaleneacetic acid (0.5 mg $L^{-1}$) and 6-benzylaminopurine (2.0 mg $L^{-1}$). In vitro rooting of the micros hoots was maximum in the medium supplemented with indolebutyric acid at 2.0 mg $L^{-1}$. The well-rooted microshoots could be successfully hardened and transplanted in the field. This result can be reproduced and is a viable protocol for successful clonal propagation of the seedless triploid Acorus calamus for conservation and sustainable development.
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Clonal Propagation of Triploid Acorus calamus Linn. Using Dual-Phase Culture System
Ningthoujam Sandhyarani,Gurumayum Jitendra Sharma,Rajkumar Kishor 한국작물학회 2011 Journal of crop science and biotechnology Vol.14 No.3
Acorus calamus is an important medicinal plant which has been used in Indian traditional medicine since time immemorial. Various bioactive molecules, viz., acorin, α- and β–asarone, asaryldehyde, caryophylene, isoasarone, methylisoeugenol, and safrol have been isolated from this plant. However, the use of this plant for medicinal purpose has been recently banned due to the high toxic property of β-asarone. The triploid Acorus calamus is reported to be low in β-asarone content and thus found to be the ideal raw material for medicinal use. The present investigation represents our finding for successful in vitro clonal propagation of the elite triploid accessions of Acorus calamus for mass propagation. In the dual-phase culture system consisting of agar-solidified Murashige and Skoog medium overlaid by liquid fraction of the same medium, maximum multiple shoot induction was favored by supplementation of α-naphthaleneacetic acid (0.5 mg L^(-1) ) and 6-benzylaminopurine (2.0 mg L^(-1) ). In vitro rooting of the microshoots was maximum in the medium supplemented with indolebutyric acid at 2.0 mg L^(-1) . The well-rooted microshoots could be successfully hardened and transplanted in the field. This result can be reproduced and is a viable protocol for successful clonal propagation of the seedless triploid Acorus calamus for conservation and sustainable development.
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Microrhizome Induction in Acorus calamus Linn. - An Important Medicinal and Aromatic Plant
Ningthoujam Sandhyarani Devi,Rajkumar Kishor,Gurumayum Jitendra Sharma 한국원예학회 2012 Horticulture, Environment, and Biotechnology Vol.53 No.5
An effective protocol for in vitro microrhizome induction was developed for Acorus calamus. The explants,rhizome axillary buds, were cultured on dual phase Murashige and Skoog (MS) medium consisting of agar solidified phase overlaid by liquid fraction of the same medium. In this study, the effects of indole butyric acid (IBA) and α–naphthalene acetic acid (NAA) containing 2-10% (w/v) sucrose were examined on microrhizome induction. Best response was observed on the medium supplemented with 2.0 mg·L-1IBA and 60% sucrose under 16/8 hours light/dark photoperiod which produced the maximum rhizome fresh weight (0.82 g) and size (length 4.8 cm; diameter 0.55 cm) in 6weeks. The microrhizomes had 7-8 buds which were developed independent of season and each segment sprouted into roots and shoots when transplanted to soil. This protocol can be adopted for various applications, viz., large scale production of propagules of elite cytotype, in vitro conservation of the microrhizome, synthesis of secondary metabolites and for studying the biosynthetic pathways of the bioactive molecules present in the rhizomes of this important medicinal and aromatic plant.
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