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        Relation between brucellosis and husbandry practices in goats in Bangladesh

        Uddin, Muhammad Jasim,Rahman, Md Siddiqur,Hossain, Mohammad Arif,Akter, Sayeda Hasina,Majumder, S.,Park, Jin-Ho,Song, Hee-Jong The Korean Society of Veterinary Service 2007 韓國家畜衛生學會誌 Vol.30 No.2

        A study on the relation between some husbandry practices and brucellosis in goats in Bangladesh was conducted at selected areas of Mymensingh and Dhaka district, Bangladesh, from March 2005 to May 2006. Sera from 300 goats were tested by Rose bengal test (RBT), plate agglutination test (PAT), tube agglutination test (TAT) and mercaptoethanol test (MET). Out of the 300 goats, 1.670% (n=5) were positive to RBT and PAT respectively, and 2.0% (n=6) were positive to TAT and 2.33% (n=7) were positive to MET. The prevalence of brucellosis was bigger in goats reared collectively (n=2, 4%) than reared individually (n=5, 2%), and bigger in goats housed with concrete floor (n=2, 4%) than that of bare floor (n=5, 2%). The rate of brucellosis was higher in goats keep separately (n=6, 2.61%) than that of kept with other animals (n=1, 1.43%) especially with cattle. Out of 290 goats from free grazing, 7 were positive but no positive reactor(n=10) was found in non grazing goats. In conclusion, however, seroprevalence of brucellosis had no statistically significant association with rearing type, housing type and grazing or not.

      • KCI등재

        Relation between brucellosis and husbandry practices in goats in Bangladesh

        ( Muhammad Jasim Uddin ),( Md Siddiqur Rahman ),( Mohammad Arif Hossain ),( Sayeda Hasina Akter ),( S Majumder ),( Jin Ho Park ),( Hee Jong Song ) 한국가축위생학회 2007 韓國家畜衛生學會誌 Vol.30 No.2

        A study on the relation between some husbandry practices and brucellosis in goats in Bangladesh was conducted at selected areas of Mymensingh and Dhaka district, Bangladesh, from March 2005 to May 2006. Sera from 300 goats were tested by Rose bengal test(RBT), plate agglutination test(PAT), tube agglutination test(TAT) and mercaptoethanol test(MET). Out of the 300 goats, 1.67%(n=5) were positive to RBT and PAT respectively, and 2.0%(n=6) were positive to TAT and 2.33%(n=7) were positive to MET. The prevalence of brucellosis was bigger in goats reared collectively (n=2, 4%) than reared individually (n=5, 2%), and bigger in goats housed with concrete floor (n=2, 4%) than that of bare floor (n=5, 2%). The rate of brucellosis was higher in goats keep separately (n=6, 2.61%) than that of kept with other animals (n=1, 1.43%) especially with cattle. Out of 290 goats from free grazing, 7 were positive but no positive reactor(n=10) was found in non grazing goats. In conclusion, however, seroprevalence of brucellosis had no statistically significant association with rearing type, housing type and grazing or not.

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        Association study and expression analysis of olfactomedin like 3 gene related to meat quality, carcass characteristics, retail meat cut, and fatty acid composition in sheep

        Listyarini Kasita,Sumantri Cece,Rahayu Sri,Uddin Muhammad Jasim,Gunawan Asep 아세아·태평양축산학회 2022 Animal Bioscience Vol.35 No.10

        Objective: The objective of this study was to identify polymorphism in olfactomedin like 3 (OLFML3) gene, and association analysis with meat quality, carcass characteristics, retail meat cut, and fatty acid composition in sheep, and expression quantification of OLFML3 gene in phenotypically divergent sheep. Methods: A total of 328 rams at the age of 10 to 12 months with an average body weight of 26.13 kg were used. A novel polymorphism was identified using high-throughput sequencing in sheep and genotyping of OLFML3 polymorphism was performed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Among 328 rams, 100 rams representing various sheep genotypes were used for association study and proc general linear model was used to analyse association between genotypes and phenotypic traits. Quantitative real-time polymerase chain reaction (qRT-PCR) was used for the expression analysis of OLFML3 mRNA in phenotypically divergent sheep population. Results: The findings revealed a novel polymorphism in the OLFML3 gene (g.90317673 C>T). The OLFML3 gene revealed three genotypes: CC, CT, and TT. The single nucleotide polymorphism (SNP) was found to be significantly (p<0.05) associated with meat quality traits such as tenderness and cooking loss; carcass characteristics such as carcass length; retail meat cut such as pelvic fat in leg, intramuscular fat in loin and tenderloin, muscle in flank and shank; fatty acids composition such as tridecanoic acid (C13:0), palmitoleic acid (C16:1), heptadecanoic acid (C17:0), ginkgolic acid (C17:1), linolenic acid (C18:3n3), arachidic acid (C20:0), eicosenoic acid (C20:1), arachidonic acid (C20:4n6), heneicosylic acid (C21:0), and nervonic acid (C24:1). The TT genotype was associated with higher level of meat quality, carcass characteristics, retail meat cut, and some fatty acids composition. However, the mRNA expression analysis was not different among genotypes. Conclusion: The OLFML3 gene could be a potential putative candidate for selecting higher quality sheep meat, carcass characteristics, retail meat cuts, and fatty acid composition in sheep. Objective: The objective of this study was to identify polymorphism in olfactomedin like 3 (OLFML3) gene, and association analysis with meat quality, carcass characteristics, retail meat cut, and fatty acid composition in sheep, and expression quantification of OLFML3 gene in phenotypically divergent sheep.Methods: A total of 328 rams at the age of 10 to 12 months with an average body weight of 26.13 kg were used. A novel polymorphism was identified using high-throughput sequencing in sheep and genotyping of OLFML3 polymorphism was performed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Among 328 rams, 100 rams representing various sheep genotypes were used for association study and proc general linear model was used to analyse association between genotypes and phenotypic traits. Quantitative real-time polymerase chain reaction (qRT-PCR) was used for the expression analysis of OLFML3 mRNA in phenotypically divergent sheep population.Results: The findings revealed a novel polymorphism in the OLFML3 gene (g.90317673 C>T). The OLFML3 gene revealed three genotypes: CC, CT, and TT. The single nucleotide polymorphism (SNP) was found to be significantly (p<0.05) associated with meat quality traits such as tenderness and cooking loss; carcass characteristics such as carcass length; retail meat cut such as pelvic fat in leg, intramuscular fat in loin and tenderloin, muscle in flank and shank; fatty acids composition such as tridecanoic acid (C13:0), palmitoleic acid (C16:1), heptadecanoic acid (C17:0), ginkgolic acid (C17:1), linolenic acid (C18:3n3), arachidic acid (C20:0), eicosenoic acid (C20:1), arachidonic acid (C20:4n6), heneicosylic acid (C21:0), and nervonic acid (C24:1). The TT genotype was associated with higher level of meat quality, carcass characteristics, retail meat cut, and some fatty acids composition. However, the mRNA expression analysis was not different among genotypes.Conclusion: The OLFML3 gene could be a potential putative candidate for selecting higher quality sheep meat, carcass characteristics, retail meat cuts, and fatty acid composition in sheep.

      • KCI등재

        Short Communications : Seroprevalence of brucellosis in cattle in selected area of Bangladesh and comparison between Rose Bengal test and i-ELISA used for the screening of brucellosis

        ( Md. Siddiqur Rahman ),( Amitavo Chakrabartty ),( Taohidul Islam ),( Roma Rani Sarker ),( Me Alam1,Muhammad Jasim Uddin ),( Laila Akther ),( Hee Jong Song ) 한국가축위생학회 2012 韓國家畜衛生學會誌 Vol.35 No.2

        Brucellosis, a bacterial zoonoses caused by the genus Brucella is responsible for abortion and infertility in cow. Brucellosis is causing economic loss in dairy industries and prevalent worldwide including Bangladesh but limited studies are devoted to determine the prevalence and its association with reproductive factors of dairy cows in Bangladesh. Therefore, the present study was conducted to determine the seroprevalence of brucellosis in dairy cattle using screening test Rose Bengal test (RBT) and the positive sera were further confirmed by indirect- ELISA. For this purpose, a total of 400 serum samples from dairy cows with history of abortion and various reproductive disorders were collected from the Kurigram district of Bangladesh for the detection of Brucella antibody. The overall prevalence of brucellosis in dairy cattle was 2.25%. Brucellosis in cases of abortion and repeat breeding was 8.3% and 2.8%, respectively. The results shows higher prevalence of brucellosis in cases of abortion followed by repeat breeding, while there was no seropositive cases from other reproductive disorders. Age-wise seroprevalence was found 3.0% in 2∼3 years age group and 2.0% in 4∼8 years age group. The prevalence of brucellosis in indigenous and cross-bred cattle was 3.6% and 1.7%, respectively. All the animals detected positive to brucellosis by RBT were not found to be positive by i-ELISA. However, the RBT might be a suitable screening test for the diagnosis of Brucella infection in field condition in Bangladesh. These data will help to develop effective disease prevention strategies.

      • KCI등재

        Seroprevalence of brucellosis in cattle in selected area of Bangladesh and comparison between Rose Bengal test and i-ELISA used for the screening of brucellosis

        Rahman, Md. Siddiqur,Chakrabartty, Amitavo,Islam, Md. Taohidul,Sarker, Roma Rani,Alam, M.E.,Uddin, Muhammad Jasim,Akther, Laila,Song, Hee-Jong The Korean Society of Veterinary Service 2012 韓國家畜衛生學會誌 Vol.35 No.2

        Brucellosis, a bacterial zoonoses caused by the genus Brucella is responsible for abortion and infertility in cow. Brucellosis is causing economic loss in dairy industries and prevalent worldwide including Bangladesh but limited studies are devoted to determine the prevalence and its association with reproductive factors of dairy cows in Bangladesh. Therefore, the present study was conducted to determine the seroprevalence of brucellosis in dairy cattle using screening test Rose Bengal test (RBT) and the positive sera were further confirmed by indirect- ELISA. For this purpose, a total of 400 serum samples from dairy cows with history of abortion and various reproductive disorders were collected from the Kurigram district of Bangladesh for the detection of Brucella antibody. The overall prevalence of brucellosis in dairy cattle was 2.25%. Brucellosis in cases of abortion and repeat breeding was 8.3% and 2.8%, respectively. The results shows higher prevalence of brucellosis in cases of abortion followed by repeat breeding, while there was no seropositive cases from other reproductive disorders. Age-wise sero-prevalence was found 3.0% in 2~3 years age group and 2.0% in 4~8 years age group. The prevalence of brucellosis in indigenous and cross-bred cattle was 3.6% and 1.7%, respectively. All the animals detected positive to brucellosis by RBT were not found to be positive by i-ELISA. However, the RBT might be a suitable screening test for the diagnosis of Brucella infection in field condition in Bangladesh. These data will help to develop effective disease prevention strategies.

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