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RISS 인기검색어
- 검색키워드
- 저자 : Muhammad Buhari Lawan (검색결과 4 건)
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Buhari Lawan Muhammad,서요셉,기장서 한국통합생물학회 2021 Animal cells and systems Vol.25 No.6
The harmful jellyfish Cyanea nozakii Kishinouye has frequently occurred on Korean coasts, and its blooms have caused serious ecological and economic damages. DNA sequences of the C. nozakii for molecular detection and discrimination are relatively scarce. In this study, we determined the complete sequence of a single unit of tandemly repeated ribosomal DNA (rDNA) of the Korean C. nozakii and characterized the molecular features of the rDNA. The complete rDNA contained 8,003 bp (48.4% GC) with the same gene arrangement (18S, ITS1, 5.8S, ITS2, 28S, and IGS) to the typical eukaryotes. Dot plot analysis showed that the coding regions (18S, 5.8S, and 28S) were highly conserved, while the non-coding regions (ITS1, ITS2, and IGS) were more variable and parsimony-informative. The IGS contained a putative transcription termination signal (poly(T) tract) and four repeats of block minisatellites. Phylogenetic analyses using 18S and 28S rDNA revealed well-resolved relationships of C. nozakii within the order Semaeostomeae, separating it from other Cyanea species. The complete rDNA sequence provides various options for the selection of jellyfish taxonomic markers and may be useful for discriminating between species of C. nozakii and phylogeny reconstruction with close relatives.
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Muhammad, Buhari Lawan,Lee, Yeon-Su,Ki, Jang-Seu The National Institute of Biological Resources 2021 Journal of species research Vol.10 No.1
Diatoms have been used in examining water quality and environmental change in freshwater systems. Here, we analyzed molecular profiling of seasonal diatoms in the Han River, Korea, using the hypervariable region of 18S V1-V3 rRNA and pyrosequencing. Physicochemical data, such as temperature, DO, pH, and nutrients showed the typical seasonal pattern in a temperate region. In addition, cell counts and chlorophyll-a, were recorded at high levels in spring compared to other seasons, due to the diatom bloom. Metagenomic analysis showed a seasonal variation in the phytoplankton community composition, with diatoms as the most frequently detected in spring (83.8%) and winter (69.7%). Overall, diatom genera such as Stephanodiscus, Navicula, Cyclotella, and Discostella were the most frequent in the samples. However, a large number of unknown Thalassiosirales diatoms were found in spring (35.5%) and winter (36.3%). Our molecular profiling revealed a high number of diatom taxa compared to morphological observation. This is the first study of diatoms in the Han River using molecular approaches, providing a valuable reference for future study on diatoms-basis environmental molecular monitoring and ecology.
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서요셉,Buhari Lawan Muhammad,채진호,기장서 한국해양과학기술원 2021 Ocean science journal Vol.56 No.1
Blooms of the moon jellyfish (Aurelia coerulea) have been responsible for huge economic losses and environmental disruptions in oceans around the world. The mass occurrence of these jellyfish may be highly related to the benthic polyp populations; however, studies supporting this are scarce. Here, we analyzed the genetic diversity and structure of Korean A. coerulea, using two mitochondrial genes, COI and 16S rRNA, in 51 polyps collected from Jaran Bay (South Korea) that is greatly affected by the mass occurrence of the jellyfish. Genetic diversity analysis identified 20 and 9 haplotypes in COI and 16S rRNA genes, respectively. TCS network analysis and haplotype distance matrix revealed a significant genetic structure in both genes. In addition, two distinct haplogroups (A and B) coexisted in Jaran Bay, and a single haplotype (COI-H4 or 16S-H2) dominated. These findings suggest a strong population differentiation among the polyp population that may have an effect on the jellyfish blooms in Jaran Bay.
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Park Jaeyeong,Kim Taehee,Muhammad Buhari Lawan,Ki Jang-Seu 한국미생물학회 2023 The journal of microbiology Vol.61 No.6
Ship biofouling is one of the main vectors for the introduction and global spread of non-indigenous organisms. Diatoms were the early colonizers of ship hulls; however, their community composition on ships is poorly understood. Herein, we investigated the diatom community on the hull samples collected from two Korean research vessels Isabu (IRV) and Onnuri (ORV) on September 2 and November 10, 2021, respectively. IRV showed low cell density (345 cells/cm2) compared to ORV (778 cells/cm2). We morphologically identified more than 15 species of diatoms from the two research vessels (RVs). The microalgae in both RVs were identified as Amphora, Cymbella, Caloneis, Halamphora, Navicula, Nitzschia, and Plagiogramma. Of them, the genus Halamphora was found to be predominant. However, both RVs had a varied dominant species with a significant difference in body size; Halamphora oceanica dominated at IRV, and Halamphora sp. at ORV, respectively. Molecular cloning showed similar results to morphological analysis, in which Halamphora species dominated in both RVs. The hull-attached species were distinct from species found in the water column. These results revealed diatoms communities that are associated with ship hull-fouling at an early stage of biofilm formation. Moreover, ships arriving from different regions could show some variation in species composition on their hull surfaces, with the potential for nonindigenous species introduction.
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