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김문교(Moon Kyo Kim),김주혁(Ju Hyeok Kim),이미선(Mi Sun Lee),조남수(Nam Su Jo),박상익(Sang Ik Park),길진수(Jin Su Gil),Enkhtsetseg Yeruult,오하경(Ha Kyung Oh),이경희(Kyeong Hee Lee),김호방(Ho Bang Kim),이문순(Moon Soon Lee),이이(Yi 한국약용작물학회 2021 한국약용작물학회지 Vol.29 No.4
Background: Jujube (Ziziphus jujuba Mill.) friuts are rich in nutrients and an economically and ecologically important medicinal plant in Korea. However, as it is difficult to distinguish jujube cultivars using morphological characters, the development of molecular markers is necessary to protect and distinguish Korean jujube cultivars. Methods and Results: Next-generation sequencing analysis was performed on six major Korean cultivars; Bokjo, Boeun, Chuseok, Mudeung, Geumseong, and Wolchul. Sites with an insertion or deletion (InDel) were identified by comparison from the sequence information of the six Korean cultivars using the CLC Genomics Workbench. Among the identified InDels, we developed Zj-InDel-1 and Zj-InDel-2 markers, which could distinguish Bokjo, Chuseok, and Boeun from the other Korean cultivars. Conclusions: The InDel markers developed in this study could be used for classification of domestic jujube cultivars and protection of the elite ones.