High efficiency of homemade culture medium supplemented with GDF9-β in human oocytes for rescue in vitro maturation

Mohsenzadeh, Mehdi,Khalili, Mohammad Ali,Anbari, Fatemeh,Vatanparast, Mahboubeh The Korean Society for Reproductive Medicine 2022 Clinical and Experimental Reproductive Medicine Vol.49 No.2

Objective: Optimizing culture media for the incubation of immature oocytes is a vital strategy to increase the oocyte maturation rate during in vitro maturation (IVM) programs. This study evaluated the IVM and fertilization rates of human germinal vesicle (GV) and metaphase I (MI) oocytes using two different maturation media (commercial and homemade) with or without growth differentiation factor 9-β (GDF9-β). supplementation. Methods: Immature oocytes from intracytoplasmic sperm injection (ICSI) cycles were collected and assigned to one of two IVM culture media (commercial or homemade; cleavage-stage base). After maturation, MII oocytes were examined under an inverted microscope for the presence of the polar body, zona pellucida (ZP) birefringence, and meiotic spindle (MS) visualization after maturation in four conditions (commercial or homemade medium, with or without GDF9-β. ICSI was done for matured oocytes, and fertilization was confirmed by the visualization of two distinct pronuclei and two polar bodies. Results: No significant differences were found between the two culture media in terms of the time and rate of oocyte maturation or the rate of fertilization (p>0.05). Growth factor supplementation increased the 24-hour maturation rate for both GV and MI oocytes only in homemade medium. The maturation rate after 24 hours was higher for MI oocytes (p<0.05). Similar results were observed for MS visualization and ZP structure in both types of media (p>0.05). Conclusion: Higher rates of oocyte maturation and fertilization were observed after application of homemade medium supplemented with GDF9-β. Therefore, this combination may be recommended as an alternative for clinical IVM programs.

Treating Viral Diarrhea in Children by Probiotic and Zinc Supplements

Ahmadipour, Shokoufeh,Mohsenzadeh, Azam,Alimadadi, Hosein,Salehnia, Mehdi,Fallahi, Arash The Korean Society of Pediatric Gastroenterology 2019 Pediatric gastroenterology, hepatology & nutrition Vol.22 No.2

Purpose: The aim of this study was to analyze the effects of probiotics and zinc supplements on the mean duration and frequency of acute diarrhea in children aged 6 months to 2 years. Methods: In this clinical trial of infants aged between 6 months and 2 years, eligible patients were divided into 3 groups: Zinc Receiving Group (ZRG), Probiotic Receiving Group (PRG), and a control group receiving supportive care alone. The frequency of diarrhea was evaluated in the test groups during the first 24 hours and 48-72 hours, along with the duration of hospitalization and diarrhea persistence for 3-7 days. Results: Diarrhea persisted for until the third day of admission in 100% of the infants in PRG compared with only 76.1% in ZRG. The relative risk of diarrhea persistence in the PRG was 1.31 times more than in ZRG until the third day. Also, 80% of diarrhea cases in the PRG persisted until the fourth day of admission, compared with 47.8% in the ZRG group, and this value was significant. The relative incidence of diarrhea persistence in the PRG was 36.4 times greater than in the ZRG until the day 4. Also, the percentage of post-treatment complications was 35.5% in the PRG and 2.6% in the ZRG, which was significant. Conclusion: In our study, the effectiveness of zinc at a dose of 20 mg was higher than that of probiotics. The complications associated with zinc supplementation were lower than those of probiotics.

Treating Viral Diarrhea in Children by Probiotic and Zinc Supplements

Shokoufeh Ahmadipour,Azam Mohsenzadeh,Hosein Alimadadi,Mehdi Salehnia,Arash Fallahi 대한소아소화기영양학회 2019 Pediatric gastroenterology, hepatology & nutrition Vol.22 No.2

Purpose: The aim of this study was to analyze the effects of probiotics and zinc supplements on the mean duration and frequency of acute diarrhea in children aged 6 months to 2 years. Methods: In this clinical trial of infants aged between 6 months and 2 years, eligible patients were divided into 3 groups: Zinc Receiving Group (ZRG), Probiotic Receiving Group (PRG), and a control group receiving supportive care alone. The frequency of diarrhea was evaluated in the test groups during the first 24 hours and 48–72 hours, along with the duration of hospitalization and diarrhea persistence for 3–7 days. Results: Diarrhea persisted for until the third day of admission in 100% of the infants in PRG compared with only 76.1% in ZRG. The relative risk of diarrhea persistence in the PRG was 1.31 times more than in ZRG until the third day. Also, 80% of diarrhea cases in the PRG persisted until the fourth day of admission, compared with 47.8% in the ZRG group, and this value was significant. The relative incidence of diarrhea persistence in the PRG was 36.4 times greater than in the ZRG until the day 4. Also, the percentage of post-treatment complications was 35.5% in the PRG and 2.6% in the ZRG, which was significant. Conclusion: In our study, the effectiveness of zinc at a dose of 20 mg was higher than that of probiotics. The complications associated with zinc supplementation were lower than those of probiotics.

Ribose-induced Maillard Reaction as an Analytical Method for Detection of Adulteration and Differentiation of Chilled and Frozen-thawed Minced Veal

Masoumeh Akbarabadi,Mohammad Mohsenzadeh,Mohammad-Reza Housaindokht 한국축산식품학회 2020 한국축산식품학회지 Vol.40 No.3

Quality control of meat products is one of the main concerns of consumers, governmental control authorities, and retailers. The purpose of this study was to employ ribose-induced Maillard reaction in detection of meat adulteration and differentiation of fresh-chilled from frozen-thawed minced veal. The browning intensity was assessed through measuring the absorbance at 420 nm with a spectrophotometer as well as the direct analysis of the color and pH. The results showed that CIE b*, CIE a*, and A420* values in the extract of fresh-chilled veal were significantly (p<0.05) higher than frozenthawed samples. The extract of frozen meat samples stored at –18℃ became significantly darker and more yellowish compared to –4℃. The results showed that the A420* value in the frozen-thawed veal stored at −4℃ and −18℃ was reduced by approximately 17.22±3.53% and 11.68±2.49%, respectively, compared with fresh-chilled veal. The findings also showed that the storage temperature of minced veal and the heating time in this reaction had a significant effect on all tested variables (p<0.0001). The proposed method can be considered as an easy, quick, and inexpensive test for differentiating between the fresh-chilled and frozen-thawed minced veal.

Heterogeneous SnCl_2/SiO_2 versus Homogeneous SnCl_2 Acid Catalysis in the Benzo[N,N]-heterocyclic Condensation

Hossein Reza Darabi,Kioumars Aghapoor,Farshid Mohsenzadeh,Mohammad Reza Jalali,Shiva Talebian,Leila Ebadi-Nia,Ehsan Khatamifar,Ali Aghaee 대한화학회 2011 Bulletin of the Korean Chemical Society Vol.32 No.1

The scope of homogeneous Lewis acid-catalyzed benzo[N,N]-heterocyclic condensation was expanded to include the use of various metal salts not reported in the literature and SnCl_2·2H_2O was finally selected. Among various solid supports activated with SnCl_2, heterogeneous SnCl_2/SiO_2 proved to be the most effective and significantly higher conversions were achieved compared to SnCl_2·2H_2O itself. The results of TG-DTA and BET indicated that dispersed SnCl_2 coordinates with surface hydroxyl groups of silica leading to formation of stable Lewis acid sites. Low catalyst loading, operational simplicity, practicability and applicability to various substrates render this eco-friendly approach as an interesting alternative to previously applied procedures.

Heterogeneous SnCl₂/SiO₂ versus Homogeneous SnCl₂ Acid Catalysis in the Benzo[N,N]-heterocyclic Condensation

Darabi, Hossein Reza,Aghapoor, Kioumars,Mohsenzadeh, Farshid,Jalali, Mohammad Reza,Talebian, Shiva,Ebadi-Nia, Leila,Khatamifar, Ehsan,Aghaee, Ali Korean Chemical Society 2011 Bulletin of the Korean Chemical Society Vol.32 No.1

The scope of homogeneous Lewis acid-catalyzed benzo[N,N]-heterocyclic condensation was expanded to include the use of various metal salts not reported in the literature and $SnCl_2{\cdot}2H_2O$ was finally selected. Among various solid supports activated with $SnCl_2$, heterogeneous $SnCl_2/SiO_2$ proved to be the most effective and significantly higher conversions were achieved compared to $SnCl_2{\cdot}2H_2O$ itself. The results of TG-DTA and BET indicated that dispersed $SnCl_2$ coordinates with surface hydroxyl groups of silica leading to formation of stable Lewis acid sites. Low catalyst loading, operational simplicity, practicability and applicability to various substrates render this eco-friendly approach as an interesting alternative to previously applied procedures.

Allelopathic Potential of Select Gymnospermous Trees

da Silva, Jaime A. Teixeira,Karimi, Javad,Mohsenzadeh, Sasan,Dobranszki, Judit Institute of Forest Science 2015 Journal of Forest Science Vol.31 No.2

Allelopathy is an ecological phenomenon that refers to the beneficial or harmful effects of one plant on another plant, both crop and weed species, by the release of organic chemicals (allelochemicals) from plant parts by leaching, root exudation, volatilization, residue decomposition in soil and other processes in both natural and agricultural systems. Allelopathy can affect many aspects of plant ecology including occurrence, growth, plant succession, the structure of plant communities, survival, dominance, diversity, and plant productivity. In this review, we describe the concept of allelopathy, some mechanisms of operation within plants and then focus on a select number of gymnospermous tree genera: Ephedra, Pinus, Taxus, Cedrus, Juniperus, Picea, Cunninghamia and Araucaria. Pinus, Taxus (yew) and Cedrus (cedar) trees have a strong negative allelopathic effect on the germination, growth, or development of other plant species in the forest community.

Effect of Synbiotic on the Treatment of Jaundice in Full Term Neonates: A Randomized Clinical Trial

Ahmadipour, Shokoufeh,Baharvand, Parastoo,Rahmani, Parisa,Hasanvand, Amin,Mohsenzadeh, Azam The Korean Society of Pediatric Gastroenterology 2019 Pediatric gastroenterology, hepatology & nutrition Vol.22 No.5

Purpose: Jaundice accounts for most hospital admissions in the neonatal period. Nowadays, in addition to phototherapy, other auxiliary methods are used to reduce jaundice and the length of hospitalization. This study aimed to investigate the effect of probiotics on the treatment of hyper-bilirubinemia in full-term neonates. Methods: In this randomized clinical trial, 83 full-term neonates, who were admitted to the hospital to receive phototherapy in the first 6 months of 2015, were randomly divided into two groups: synbiotic (SG, n=40) and control (CG, n=43). Both groups received phototherapy but the SG also received 5 drops/day of synbiotics. Serum bilirubin, urine, stool, feeding frequency, and weight were measured daily until hospital discharge. A p-value<0.05 was considered statistically significant. Results: The mean total serum bilirubin in the SG was lower than that in the CG ($9.38{\pm}2.37$ and $11.17{\pm}2.60mg/dL$, respectively). The urine and stool frequency in the SG was significantly higher than that in the CG (p<0.05). The duration of hospitalization in the SG was shorter than that in the CG. Conclusion: Use of synbiotics as an adjuvant therapy had a significant treatment effect on jaundice in full-term neonates. Further studies including larger samples with long follow-up periods are essential to confirm the benefits of routine use of synbiotics in neonatal patients with jaundice.

Effect of Synbiotic on the Treatment of Jaundice in Full Term Neonates: A Randomized Clinical Trial

Shokoufeh Ahmadipour,Parastoo Baharvand,Parisa Rahmani,Amin Hasanvand,Azam Mohsenzadeh 대한소아소화기영양학회 2019 Pediatric gastroenterology, hepatology & nutrition Vol.22 No.5

Purpose: Jaundice accounts for most hospital admissions in the neonatal period. Nowadays, in addition to phototherapy, other auxiliary methods are used to reduce jaundice and the length of hospitalization. This study aimed to investigate the effect of probiotics on the treatment of hyper-bilirubinemia in full-term neonates. Methods: In this randomized clinical trial, 83 full-term neonates, who were admitted to the hospital to receive phototherapy in the first 6 months of 2015, were randomly divided into two groups: synbiotic (SG, n=40) and control (CG, n=43). Both groups received phototherapy but the SG also received 5 drops/day of synbiotics. Serum bilirubin, urine, stool, feeding frequency, and weight were measured daily until hospital discharge. A p-value<0.05 was considered statistically significant. Results: The mean total serum bilirubin in the SG was lower than that in the CG (9.38±2.37 and 11.17±2.60 mg/dL, respectively). The urine and stool frequency in the SG was significantly higher than that in the CG (p<0.05). The duration of hospitalization in the SG was shorter than that in the CG. Conclusion: Use of synbiotics as an adjuvant therapy had a significant treatment effect on jaundice in full-term neonates. Further studies including larger samples with long follow-up periods are essential to confirm the benefits of routine use of synbiotics in neonatal patients with jaundice.

Allelopathic Potential of Select Gymnospermous Trees

Jaime A. TEIXEIRA da SILVA,Javad KARIMI,Sasan MOHSENZADEH,,Judit DOBRÁNSZKI 강원대학교 산림과학연구소 2015 Journal of Forest Science Vol.31 No.2

Allelopathy is an ecological phenomenon that refers to the beneficial or harmful effects of one plant on another plant, both crop and weed species, by the release of organic chemicals (allelochemicals) from plant parts by leaching, root exudation, volatilization, residue decomposition in soil and other processes in both natural and agricultural systems. Allelopathy can affect many aspects of plant ecology including occurrence, growth, plant succession, the structure of plant communities, survival, dominance, diversity, and plant productivity. In this review, we describe the concept of allelopathy, some mechanisms of operation within plants and then focus on a select number of gymnospermous tree genera: Ephedra, Pinus, Taxus, Cedrus, Juniperus, Picea, Cunninghamia and Araucaria. Pinus, Taxus (yew) and Cedrus (cedar) trees have a strong negative allelopathic effect on the germination, growth, or development of other plant species in the forest community.