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Xianfeng Gong,Minwei Wang,Shin-ichi Tashiro,Satoshi Onodera,Takashi Ikejima 생화학분자생물학회 2006 Experimental and molecular medicine Vol.38 No.4
A terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) assay was used to determine that apoptosis causes HeLa cell death induced by pseudolaric acid B. The c-Jun N-terminal kinase (JNK) inhibitor SP600125 decreased p53 protein expression during exposure to pseudolaric acid B. SP600125 decreased the phosphorylation of p53 during pseudolaric acid B exposure, indicating that JNK mediates phosphorylation of p53 during the response to pseudolaric acid B. SP600125 reversed pseudolaric acid B-induced down-regulation of phosphorylated extracellular signal-regulated pro-tein kinase (ERK), and protein kinase C (PKC) was activated by pseudolaric acid B, whereas stau-rosporine, calphostin C, and H7 partly blocked this effect. These results indicate that p53 is partially regulated by JNK in pseudolaric acid B-induced HeLa cell death and that PKC participates in pseudolaric acid B-induced HeLa cell death.
Sequence Analysis of Mitochondrial Genome of Toxascaris leonina from a South China Tiger
Kangxin Li,Fang Yang,A. Y. Abdullahi,Meiran Song,Xianli Shi,Minwei Wang,Yeqi Fu,Weida Pan,Fang Shan,Wu Chen,Guoqing Li 대한기생충학열대의학회 2016 The Korean Journal of Parasitology Vol.54 No.6
Toxascaris leonina is a common parasitic nematode of wild mammals and has significant impacts on the protection of rare wild animals. To analyze population genetic characteristics of T. leonina from South China tiger, its mitochondrial (mt) genome was sequenced. Its complete circular mt genome was 14,277 bp in length, including 12 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and 2 non-coding regions. The nucleotide composition was biased toward A and T. The most common start codon and stop codon were TTG and TAG, and 4 genes ended with an incomplete stop codon. There were 13 intergenic regions ranging 1 to 10 bp in size. Phylogenetically, T. leonina from a South China tiger was close to canine T. leonina. This study reports for the first time a complete mt genome sequence of T. leonina from the South China tiger, and provides a scientific basis for studying the genetic diversity of nematodes between different hosts.
Modification and Characterization of Nano-TiO2 for Efficient Fixation on Cotton Fibers
Min Wei,Shugen Wang,Chang Sun,Change Zhou,Jiadong Ni 한국섬유공학회 2018 Fibers and polymers Vol.19 No.11
In this paper, a silane coupling agent, 3-aminopropyltriethoxysilane, was reacted with nano-TiO2 to introduce amino group onto it which was then reacted with trichlorotriazine to obtain a dichlorotriazine functionalized nano-TiO2 for the firm fixation of it on cotton fibers. The reaction process was monitored by the titration of primary and secondary amino groups, and the reaction conditions were optimized with orthogonal method accordingly. The dichlorotriazine functionalized nano-TiO2 was reacted with cotton fabric by the nucleophile substitution reaction to afford nano-TiO2 functionalized cotton fabric, the structure and surface morphology of the nano-TiO2 finished cotton fibers were studied by FT-TR and SEM. In addition, the fixation duration of the nano-TiO2 modified cotton was studied according to AATCC test method 61-2010. The results show that the washing fastness of the nano-TiO2 is excellent.