Histone deacetylase 3 is selectively involved in L3MBTL2-mediated transcriptional repression

Yoo, Jung-Yoon,Choi, Kyung-Chul,Kang, HeeBum,Kim, Young Jun,Lee, Jeongmin,Jun, Woo Jin,Kim, Mi-Jeong,Lee, Yoo-Hyun,Lee, Ok-Hee,Yoon, Ho-Geun Elsevier 2010 FEBS letters Vol.584 No.11

<P><B>Abstract</B></P><P>This is the first report that L(3)mbt-like 2 (L3MBTL2) specifically interacts with the histone deacetylase domain of histone deacetylase 3 (HDAC3) via its MBT domain. Here, we show that L3MBTL2 selectively interacts with HDAC3, but not other class I HDACs. An in vitro peptide-binding assay demonstrated the specific association of HDAC3 with methylated histone-K20 tail and L3MBTL2. Furthermore, depletion of HDAC3 resulted in a decrease of methylated K20-H4, as well as an increase in acetylated histone H3. Consequently, HDAC3 knock-down selectively suppressed L3MBTL2-mediated transcriptional repression. Taken together, our results reveal the concerted action of both HDAC3 and L3MBTL2 in histone deacetylation and methylation-dependent transcriptional repression.</P><P><B>Structured summary</B></P><P>MINT-7719975: <I>L3MBTL2</I> (uniprotkb:Q969R5) and <I>HDAC3</I> (uniprotkb:O15379) <I>colocalize</I> (MI:0403) by <I>fluorescence microscopy</I> (MI:0416)</P><P>MINT-7719941, MINT-7719921: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>binds</I> (MI:0407) to <I>HDAC3</I> (uniprotkb:O15379) by <I>pull down</I> (MI:0096)</P><P>MINT-7719991: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-7719958: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>physically interacts</I> (MI:0915) with <I>HDAC3</I> (uniprotkb:O15379) by <I>anti tag coimmunoprecipitation</I> (MI:0007)</P><P>MINT-7719897: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>two hybrid</I> (MI:0018)</P>

최승희 무용예술을 활용한 중·고등학교 다문화무용교육 프로그램 개발연구

유미희 ( Yoo Mi-hee ) 한국무용예술학회 2016 무용예술학연구 Vol.61 No.4

The purpose of this study was to develop a multicultural dance education program utilizing Choi Seung hee’s dance art on 34 students in a middle school and 33 students in a high school in Seoul. Based on the model by Lee Sang gab (Preparation, Development and Improvement), a multicultural dance education program was developed through literature study and field study. The multicultural dance education program was modified and supplemented by the evaluation of experts and students, and the final program was developed as 11 units for middle school and 7 units for high school.

최승희 무용예술의 다문화무용교육표준에 관한 연구

유미희 ( Yoo Mi-hee ) 한국무용예술학회 2014 무용예술학연구 Vol.51 No.6

The objective of this study was to establish a multicultural dance education standard in elementary and secondary schools, using Choi Seung hee’s dance art as a teaching medium. The plan was drafted first through a specialist council, and the validity of the contents were tested through a questionnaire survey of 15 advisors. The results of this study were as follows. First, in the elementary school, the expectation level of communication in the1st the 2nd grades, the expectation level of performance and creation in the 5th and 6th grades, and the goals and expectation level of communication in the 5th and 6th grades were found inadequate. Second, in the middle school, all the goals of performance, creation, appreciation, and communication were adequate. Third, in the high school, all the goals and expectation levels of performance, creation, appreciation, and communication were adequate.

최승희 무용예술을 활용한 초등학교 다문화 무용교육 프로그램 개발연구

유미희 ( Yoo Mi-hee ) 한국무용예술학회 2015 무용예술학연구 Vol.54 No.3

The objective of this study was to develop a multicultural dance education program for elementary school children using Choi Seung hee’s dance art. As to research methods, this study planned the lessons of the multicultural dance program applicable to elementary school children using sources retrieved from literature review and field studies. Based on Lee Sang gap’s model, the program was designed to have three stages (preparation, development, and improvement). The goals and contents of learning were organized according to the five themes presented in the preparation stage, and 10 sessions of multicultural dance education were designed. In the improvement stage, multicultural dance education expanded to 12 sessions for an effective learning.

민들레 추출액 농도에 따른 민들레 코팅쌀밥의 품질에 관한 연구

유경미,이연경,김세희,황인경,이부용,김성수,홍희도,김영찬 한국조리과학회 2005 한국식품조리과학회지 Vol.21 No.1

Washed rice was coated by spraying aqueous dandelion (Taraxacum officinale) extracts at 20, 30, 40 'Brix and dried at room temperature. The coating procedure was conducted 5 mL/min for 10min. Property changes in the dandelion coated rice and un-coated cooked rice were observed during 2 days of storage. Increasing the coating concentration was associated with lower color values (L, a value) and moisture contents. Sensory and texture properties generally decreased in the cooked rice coated with dandelion more than the control (non-coated rice). But, D-20(20 °Brix of the dandelion concentration) resulted in better textural properties and s ensnry scores for hardness and overall acceptability in cooked rice. Compared to the control, the washed rice roated with 20 °Brix for 0 and 2 days showed better quality in sensory activities, a lower gel consistency and better textural activities. It was concluded that the optimum coating ration of rice and added dandelion extract for cooking were 20 °Brix and 5% respectively, in proportion to the total weight of raw rice.

한국춤문화의 구성원리에 관한 연구 : 춘앵전과 살풀이에 나타난 삼재론적 음양오행을 중심으로 With Special Reference to the Cosmic Dual Forces and the Five Elements appeared in Choonaengjun and Salpuri(an exorcist dance) in a View of a Theory of the Three Disasters

유미희 韓國舞踊敎育學會 1999 韓國舞踊敎育學會誌 Vol.10 No.2

The purpose of this study is, as a first task, in order to newly recognize the value of Korean dance as a centripetal force of Korean culture in consideration of an era of culture and industry which will come very soon, and also in order to provide for establishing the art of Korean dance which will exerts a culture-sucking force in the huge waves of globalization, to study and review the constituent principle of Korean dance culture. For such a study, in the first place, a typical court dance of a traditional society. Choonaengjun, and a folk dance, Salpuri(an exorcist dance) were selected, and some ideological characteristics of tem were drawn out from an approach to see how they are structured. IN a structural approach of dance by which dance Is reviewed in its structure, dance was reviewed in a macroscopic analysis and a microscopic analysis. In the macroscopic analysis of dance, the relations between the structural content of dance and the importance of aesthetics or significance(meaning) were reviewed, thereby drawing out various themes concerning the form and content of the constituent elements combined in dance performance. In such a stage of a macroscopic analysis, the meaning of the correlations between various elements through the four certain media of dance, i.e., movement content, performeer, visual performance, and sound accompaniment type, which as a unified body is given to dance, was yield. In the meantime in a microscopic analysis, the structure and characteristic of dance movement itself were analysed. Through an analysis of the internal characteristic of dance movement, various body expressions of dance movement were characterized, thereby providing or suggesting the elements of movement which influence the configuration of dance movement. In contrast to that analyssi of the internal characteristic of dance movement, in a structural analysis of the external aspect of dance movement, the direction of the movement of a body part ; height; rhythm; weight; type of weight support; level change; direction in a space; the time elapsed in movement; touch pattern; orientataion; and floor pattern were described and depicted, so that the content of the kinetic senses in all the form of dance movement and style could be understood(Shin, Sang Mi;2) After all, in this study, the ideological significance or meaning contained in dance movement was studied and reviewed; however, since this study was centered on a literature study and a phenomenologic analysis, it is considered that suggestion of the scientific objectivity is somewhat limited.

한국 무용교육의 변천과 과제 : 해방이후를 중심으로

유미희 韓國舞踊敎育學會 2009 韓國舞踊敎育學會誌 Vol.20 No.2

The objective of this study was to understand the current state of dance education in Korea and prospect its future by examining the development of Korean dance education in response to historical and social changes as the factors of educational environment. Through literature review and field surveys, I studied school dance education based on the government's education policies, other dance education activities outside school, and the trends of research on dance education in academic circles, and presented the results and problems of dance education in Korea. In the results of this study, dance education in Korea can be divided into three periods according to the government's education policies. The first period is the early period (1945∼1961) when the concept of education dance and the perception of professional dance education were established with the foundation of the Chosun Education Dance Research Institute. The second period is the authoritarian period (1961∼1992) from 1960 to the beginning of the 1990s when dance education was fully institutionalized. The third period was the democratization period (1993∼2008) when 'the publicness of dance education' was emphasized. In this way, from the 1^(st) National Curriculum to the present, dance education in Korea has been regarded as an area of physical education in elementary and secondary education, and its contents have been gradually developed into an art curriculum. In educational policies or systems, however, there are problems to be solved as follows. First, in the aspect of educational equality, opportunities for dance education should be provided to all people with none excluded. Second, the dance curriculum should be specialized. Dance education, which is regarded as a part of physical education, cannot produce its educational and artistic values in terms of the contents and operation of education. Thus, for the specialty and identity of the dance curriculum, dance education should be separated from physical education and secure its specialty and autonomy in the contents and operation of education. Third, the differentiation of advanced dance education is required in order to cope with changing needs of society and to provide productive job opportunities. Each university should have distinguished education goals and construct dance infrastructure in each area for raising creative international manpower. Fourth, dance capitals should be expanded for lifelong education. When the four solutions are implemented, dance education in Korea will display its strength and influence.

진주 포구락과 교방 굿거리춤에 나타난 풍류정신 연구

유미희 韓國舞踊敎育學會 2000 韓國舞踊敎育學會誌 Vol.11 No.2

This study is to see what relevancy the elegant pursuits of Korean ancestors which have become a spiritual and eultural background of this country has to its dance culture, and te investigate some aspects of the Korean peeples elegant pursuits through Jiniu pokurak and Kyobang gut-guri dances. For thin purpose, structures and characteristics of those to dances such as dance movement, dance floor pattenL and the movement quality are phenomenologically analyzed. In Jin;upoku-rak, the circular and S-shapes formation and the dance evolvement into all directions symbolize the cycle of nature and the natural lawn in all things. Nature does'nt exclude human being. On the contrary, it embraces ua with its power of giving life and has deep relation to the interactions between Yin and Yang. Meanwhile, in the Gutguri dance, the Yin and Yang structure of tension and relaxation which can be seen in the "il"shaped formation, the taegeuk patterns in the wrist gestures and dance floor pattern, and the circulation of vital energy in the vertical body posture represent the peculiar Korean idea understanding the heaven as a fountain of the inherent human vitality and will to accomplish a strate of self·renunciation, absence of the worldly desirers by feeling the rhythm of life that nature invokes, harmonizing the rhythm of life inside man with that inside nature, and wholly entrusting our self to the dispensation of nature. The Confucianistic ideal that we can have a fortunate and happy alee by contmlling certain human dispositions: free and extravagant playfulness coming from realistic mimic movements, getting vitality by breaking away from daily routine through bold and active small drum dance, all of these indicate the Korean Pung Ruy taste that allows the fletible basic attributes and changes of nature as they are and bold deviation from everyday life for stepping in a new world.

고등급 보안 시스템을 위한 IPSec 시스템 기능 명세

유희준,김영미,최진영,서동수,노병규 고려대학교 컴퓨터과학기술연구소 2001 JOURNAL OF COMPUTER SCIENCE & ENGINEERING TECHNOLO Vol.3 No.-

현대 사회는 인터넷 사용자의 증가와 제반 기술의 비약적인 발전으로 사회활동 전반에 대한 인터넷 기반 서비스가 이루어지고 있다. 이런 과정에서 발생한 중요한 문제중의 하나가 불특정 다수가 자유로이 통신을 하기 위해 개발된 인터넷상에서 사용자의 보안을 보장해주는 것이다. 최근에 와서 인터넷상에서 개인 정보유출로 인한 많은 피해가 발생하면서 정보보호에 대한 관심이 더욱 증가하고 있다. 현재 이 문제를 해결하기 위하여 많은 보안 관련 시스템들이 개발되고 있으며, 세계 각 국의 표준 기구에서는 이러한 시스템에 대한 등급을 평가하고 있다. 각 등급을 살펴보면, 시스템 개발 초기부터 정형기법을 이용하여 개발되어진 시스템들이 고등급을 획득하고 있다. 국내에서도 한국정보보호센터(KISA)에서 보안 시스템에 대한 등급을 평가를 수행하면서 정형기법을 이용하여 땅세 검증된 시스템에게 K5이상의 고등급을 부여하고 있다. 이러한 등급을 살펴보면, 시스템의 기능을 정형명세언어를 이용해서 명세하는 부분을 공통적으로 요구하고 있다. 본 논문에서는 높은 보안 등급의 IPSec 관련 시스템을 개발하기 위해서 정형명세언어인 Z를 이용해서 IPSec의 동작을 명세하였다. The modern community is moving across the whole areas based on Internet with the growing Internet users and the great technological development. One of the important things that can be problem in this community is that the Internet should assure secure networking and security mechanism for the many security-ignorant users. Recently, Due to a lot of damages like a personal drain, security concerns is growing very rapidly. Now, many security application system is developing to solve this problem and each of the world standard organizations is evaluating these security application systems. Considering the each levels, we found that system using formal methods from the initial system development process acquired the high-level. In domestic, KISA manages the evaluation of security system and gives the higher than K5 to the system that specified and verified using formal methods. In this all levels, it should be required the system specification part. In this paper, we specified IPSec using formal specification language Z to develope the high-level IPSec system.

한국창작춤에서의 전통의 현대적 의미에 관한 연구

유미희 韓國舞踊敎育學會 1998 韓國舞踊敎育學會誌 Vol.9 No.2

Through the study, I have tried to investigate the possibility of development of the traditional Korean dance with cultural identity of 2000's through the consideration of art creative change of Korean traditional dance which is turning into the creative roots of modern Korean dance. The objective of this study was Korean creative dance which is becoming the core of art dance activity. And creative dance means those kinds of dances which are created as a modern dance from traditional Koran dance and all the creative dances of modern times which can be acceptable as western modernism from the period of new dance to 1990's. The traditional issue of Korean dance which has decorated modern contemporary Korean dance history has developed and stagnated repeatedly under the motto of groping for the cultural identity in the process of the initiation of western culture. The new dance in 1930's and the creative dance in 1970's were the results of the respect of tradition and the reactionary tendency, and the modernization of the korean tradition has been the artistic mission which has ruled the modern comtemporary Korean dance history. As the Korean dance in 1990's, showing the revolution to the tradition, has received the tradition aggressively, the modernization of the traditional Korean dance has been showed to be the periodical issue through the transplanting process applicable to the reality. In conclusion, the cultural meaning of the traditional Korean dance in this periodical atmosphere of the globalism shall be found through not the simple reproduction of following of the tradition itself but the development of a new tradition of Korean dance. So the entry into the global dance of Korean dance is thought to be possible only with a future oriented vision.