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      • KCI등재

        Optimization of Walnut Tissue Culture Medium Using Seed Composition

        Elahe Najafian Ashrafi,Kourosh Vahdati,Hassan Ebrahimzadeh,Masoud Mirmasoumi,Naser Lotfi 한국원예학회 2009 Horticulture, Environment, and Biotechnology Vol.50 No.2

        Micropropagation of walnut has been partially limited to the lack of an appropriate culture medium. We hypothesized that the minerals in proportions similar to those found in the walnut seeds could provide an optimum medium for shoot micropropagation. The mineral composition of five walnut seeds as ‘Serr’, ‘Pedro’, ‘Lara’, ‘Hartley’ and ‘Rond e Montignac’ were analyzed by Inductive Couple Plasma-Omission Spectroscopy (ICP-OES). In addition, to study the effect of Cu and myo-inositol on shoot length and rooting rate of explants, these factors were examined. Concentrations of mineral compositions of walnut seeds were several times of those in DKW (Driver and Kuniyuki Walnut medium). Based on minimum concentration of seeds, two new media (as ×1.5 and ×2 DKW) were formulated. Growth of explants of ’Hartley‘, ‘Sunland’ and ‘Vina’ cultivars on two new media was compared to those cultured on DKW medium. The explants cultured on ×2 DKW and ×1.5 DKW formed shoots having green-colored leaves but produced callus similar to those cultured on DKW medium. Better growth was observed on ×1.5 DKW medium. Potential multiplication rate was different among the cultivars and ‘Vina’ had the maximum stem length and number of auxiliary buds on ×1.5 DKW. The results of the second experiment indicated that number of auxiliary buds per shoot and stem length were enhanced in higher levels of Cu and myo-inositol. In vitro rooting percentage for ‘Sunland’, ‘Hartley’ and ‘Vina’ were 50, 31 and 16% in the DKW medium, respectively but decreased to 5-17% in two modified media (×1.5 and ×2 DKW). A better rooting (up to 70%) was obtained when shoots were placed in higher levels of Cu and myo-inositol (CM2). The results suggested that some of the walnut cultivars need to grow on higher mineral concentrations of DKW medium. In addition, the higher levels of Cu and myo-inositol would be affective on growth rate and rooting percentage of walnut explants.

      • KCI등재

        Using hairy roots for production of secondary metabolites in Artemisia

        Zahra Mottaki,Maryam Rezayian,Vahid Niknam,Hassan Ebrahimzadeh,Masoud Mirmasoumi 한국식물생명공학회 2019 Plant biotechnology reports Vol.13 No.3

        Secondary metabolites are a group of chemical compounds in plants which have various functions and applications in different industries such as leather and pharmaceutical products. Artemisia is a genus of important vascular plants which contains many species. There are many chemical compounds in Artemisia which are still unknown. Additionally, hairy root culture in plants is considered to be one of the new techniques applied to production of secondary metabolites which denotes the vast application of this technique. Hairy root culture as a differentiated tissue has been under consideration for many researchers which is because of its genetic stabilization; its rapid growth in a hormone-free medium is also very important. The current study is conducted with the objective of hairy root induction in explant parts of some Artemisia species with various strains of Agrobacterium rhizogenes. The results show that total protein content and enzyme activity during hairy root induction has an ascending procedure. Phenol and flavonoid content have shown considerable increase. The sterol and essential oil have also shown interesting consistency towards each other. To the best of our knowledge, this is the first report of production secondary metabolites and antioxidant activities by hairy root-derived Artemisia. This study may open possibilities for production of the pharmaceutically high-value secondary metabolites.

      • KCI등재

        Experimental determination of void fraction in surface aeration using image processing technique

        Amir Mohammadpour,Mohammad Ali Akhavan-Behabadi,Masoud Ebrahimzadeh,Pedram Hanafizadeh,Mehrdad Raisee 대한기계학회 2015 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.29 No.6

        In this paper, a new method for determination of void fraction in surface aeration process is presented and discussed. The proposedmethod is based on the image processing technique. The experimental setup has been designed to create various surface aeration conditionsin the water. Void fraction has been calculated for the wide range of water height, impeller immersion depth and rotational speed. Experiments have been performed in an open cubic tank with side length of 60 cm, equipped with one Rushton disk turbine. Moreover,the void fraction has been measured with level gauge method. The results showed that the image processing technique provides moreaccurate results than the level gauge measurements for void fraction calculation in surface aeration especially in low void fraction aeration. In addition, the experimental data revealed that increase in impeller immersion depth and rotational speed increase void fraction andoxygen transfer rate in surface aeration process.

      • KCI등재

        Conus Medullaris Teratoma: Case Report and Literature Review

        ( Ehsan Keykhosravi ),( Amin Tavallaii ),( Hamid Rezaee ),( Kaveh Ebrahimzadeh ),( Masoud Pishjoo ),( Reza Abdi ),( Mona Maftouh ),( Behzad Aminzadeh ) 대한뇌종양학회 대한신경종양학회 2020 Brain Tumor Research and Treatment Vol.8 No.2

        Teratomas are benign germ cell tumors that usually found out of their anatomical origin. Teratomas usually are found in sacrococcygeal area, gonads, mediastinum, cervicofacial region and intracranial fossa. Spinal teratomas are rare. In this study we describe a case of conus medullaris teratoma which was diagnosed based on imaging studies. The patient underwent surgery. We did bilateral laminectomy. The mass lesion had an obvious and rigid attachment to the conus medullaris. The wall of the lesion was resected as much as possible, but total resection of the lesion’s wall could not be done due to changes in neural monitoring. Previous related studies are reviewed.

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