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Mardina, Primata,Li, Jinglin,Patel, Sanjay K.S.,Kim, In-Won,Lee, Jung-Kul,Selvaraj, Chandrabose The Korean Society for Microbiology and Biotechnol 2016 Journal of microbiology and biotechnology Vol.26 No.7
Methanol is a versatile compound that can be biologically synthesized from methane (CH<sub>4</sub>) by methanotrophs using a low energy-consuming and environment-friendly process. Methylocella tundrae is a type II methanotroph that can utilize CH<sub>4</sub> as a carbon and energy source. Methanol is produced in the first step of the metabolic pathway of methanotrophs and is further oxidized into formaldehyde. Several parameters must be optimized to achieve high methanol production. In this study, we optimized the production conditions and process parameters for methanol production. The optimum incubation time, substrate, pH, agitation rate, temperature, phosphate buffer and sodium formate concentration, and cell concentration were determined to be 24 h, 50% CH<sub>4</sub>, pH 7, 150 rpm, 30℃, 100 mM and 50 mM, and 18 mg/ml, respectively. The optimization of these parameters significantly improved methanol production from 0.66 to 5.18 mM. The use of alginate-encapsulated cells resulted in enhanced methanol production stability and reusability of cells after five cycles of reuse under batch culture conditions.
( Primata Mardina ),( Jinglin Li ),( Sanjay K. S. Patel ),( In-won Kim ),( Jung-kul Lee ),( Chandrabose Selvaraj ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.6
Methanol is a versatile compound that can be biologically synthesized from methane (CH4) by methanotrophs using a low energy-consuming and environment-friendly process. Methylocella tundrae is a type II methanotroph that can utilize CH4 as a carbon and energy source. Methanol is produced in the first step of the metabolic pathway of methanotrophs and is further oxidized into formaldehyde. Several parameters must be optimized to achieve high methanol production. In this study, we optimized the production conditions and process parameters for methanol production. The optimum incubation time, substrate, pH, agitation rate, temperature, phosphate buffer and sodium formate concentration, and cell concentration were determined to be 24 h, 50% CH4, pH 7, 150 rpm, 30°C, 100 mM and 50 mM, and 18 mg/ml, respectively. The optimization of these parameters significantly improved methanol production from 0.66 to 5.18 mM. The use of alginate-encapsulated cells resulted in enhanced methanol production stability and reusability of cells after five cycles of reuse under batch culture conditions.
Patel, S.K.S.,Mardina, P.,Kim, D.,Kim, S.Y.,Kalia, V.C.,Kim, I.W.,Lee, J.K. Elsevier Applied Science 2016 Bioresource technology Vol.218 No.-
Raw biogas can be an alternative feedstock to pure methane (CH<SUB>4</SUB>) for methanol production. In this investigation, we evaluated the methanol production potential of Methylosinus sporium from raw biogas originated from an anaerobic digester. Furthermore, the roles of different gases in methanol production were investigated using synthetic gas mixtures of CH<SUB>4</SUB>, carbon dioxide (CO<SUB>2</SUB>), and hydrogen (H<SUB>2</SUB>). Maximum methanol production was 5.13, 4.35, 6.28, 7.16, 0.38, and 0.36mM from raw biogas, CH<SUB>4</SUB>:CO<SUB>2</SUB>, CH<SUB>4</SUB>:H<SUB>2</SUB>, CH<SUB>4</SUB>:CO<SUB>2</SUB>:H<SUB>2</SUB>, CO<SUB>2</SUB>, and CO<SUB>2</SUB>:H<SUB>2</SUB>, respectively. Supplementation of H<SUB>2</SUB> into raw biogas increased methanol production up to 3.5-fold. Additionally, covalent immobilization of M. sporium on chitosan resulted in higher methanol production from raw biogas. This study provides a suitable approach to improve methanol production using low cost raw biogas as a feed containing high concentrations of H<SUB>2</SUB>S (0.13%). To our knowledge, this is the first report on methanol production from raw biogas, using immobilized cells of methanotrophs.
Biological Methanol Production by a Type II Methanotroph Methylocystis bryophila
( Sanjay K. S. Patel ),( Primata Mardina ),( Sang-yong Kim ),( Jung-kul Lee ),( In-won Kim ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.4
Methane (CH4) is the most abundant component in natural gas. To reduce its harmful environmental effect as a greenhouse gas, CH4 can be utilized as a low-cost feed for the synthesis of methanol by methanotrophs. In this study, several methanotrophs were examined for their ability to produce methanol from CH4; including Methylocella silvestris, Methylocystis bryophila, Methyloferula stellata, and Methylomonas methanica. Among these methanotrophs, M. bryophila exhibited the highest methanol production. The optimum process parameters aided in significant enhancement of methanol production up to 4.63 mM. Maximum methanol production was observed at pH 6.8, 30°C, 175 rpm, 100 mM phosphate buffer, 50 mM MgCl2 as a methanol dehydrogenase inhibitor, 50% CH4 concentration, 24 h of incubation, and 9 mg of dry cell mass ml-1 inoculum load, respectively. Optimization of the process parameters, screening of methanol dehydrogenase inhibitors, and supplementation with formate resulted in significant improvements in methanol production using M. bryophila. This report suggests, for the first time, the potential of using M. bryophila for industrial methanol production from CH4.
Patel, S.K.S.,Selvaraj, C.,Mardina, P.,Jeong, J.H.,Kalia, V.C.,Kang, Y.C.,Lee, J.K. Applied Science Publishers 2016 APPLIED ENERGY Vol.171 No.-
<P>Both methane (CH4) and carbon dioxide (CO2) are major greenhouse gases (GHGs); hence, effective processes are required for their conversion into useful products. CH4 is used by a few groups of methanotrophs to produce methanol. However, to achieve economical and sustainable CH4 reduction strategies, additional strains are needed that can exploit natural CH4 feed stocks. In this study, we evaluated methanol production by Methylosinus sporium from CH4 and synthetic gas. The optimum pH, temperature, incubation period, substrate, reaction volume to headspace ratio, and phosphate buffer concentration were determined to be 6.8, 30 C, 24 h, 50% CH4, 1:5, and 100 mM (with 20 mM MgC12 [a methanol dehydrogenase inhibitor]), respectively. Optimization of the production conditions and process parameters significantly improved methanol production from 0.86 mM to 5.80 mM. Covalent immobilization of M. sporium on Chitosan significantly improved the stability and reusability for up to 6 cycles of reuse under batch culture conditions. The immobilized cells utilized a synthetic gas mixture containing CH4, CO2, and hydrogen (at a ratio of 6:3:1) more efficiently than free cells, with a maximum methanol production of 6.12 mM. This is the first report of high methanol production by M. sporium covalently immobilized on a solid support from a synthetic gas mixture. Utilization of cost-effective feedstocks derived from natural resources will be an economical and environmentally friendly way to reduce the harmful effects of GHGs. (C) 2016 Elsevier Ltd. All rights reserved.</P>
Patel, Sanjay K.S.,Kumar, Virendra,Mardina, Primata,Li, Jinglin,Lestari, Rowina,Kalia, Vipin C.,Lee, Jung-Kul Elsevier 2018 Bioresource technology Vol.263 No.-
<P><B>Abstract</B></P> <P>In the present study, co-cultures of the methanotrophs <I>Methylocella tundrae</I>, <I>Methyloferula stellata</I>, and <I>Methylomonas methanica</I> were evaluated for improving methanol production with their application. Among the different combinations, the co-culture of <I>M. tundrae</I> and <I>M. methanica</I> increased methanol production to 4.87 mM using methane (CH<SUB>4</SUB>) as feed. When simulated biogas mixtures were used as feed, the maximum methanol production was improved to 8.66, 8.45, and 9.65 mM by free and encapsulated co-cultures in 2% alginate and silica-gel, respectively. Under repeated batch conditions, free and immobilized co-cultures using alginate and silica-gel resulted in high cumulative production, up to 24.43, 35.95, and 47.35 mM, using simulated biohythane (CH<SUB>4</SUB> and hydrogen), respectively. This is the first report of methanol production from defined free and immobilized co-cultures using simulated biogas mixtures as feed.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The co-culture of <I>Methanotrophs</I> is effective to produce methanol using simulated biogases. </LI> <LI> The silica-gel based encapsulated co-culture is an effective approach for methanol production. </LI> <LI> This is the first report of methanol production from immobilized co-cultures using biogas. </LI> </UL> </P>
Singh, V.,Sivaramaiah, G.,Rao, J. L.,Singh, N.,Srivastava, A. K.,Singh, P. K.,Pawar, S. U.,Gao, H.,Mardina, P. Springer Science + Business Media 2016 Journal of materials science Materials in electron Vol.27 No.5
<P>Fe doped CeO2 powder was prepared using solution combustion method. Powder X-ray diffraction and scanning electron microscopy methods are used to characterize the combustion derived powder. The optical absorption spectrum exhibits three bands due to Fe3+ and Fe2+ ions. Electron paramagnetic resonance (EPR) spectrum of this sample exhibits number of resonance signals due to Fe3+ ions. The number of spins (N) participating in resonance and its paramagnetic susceptibility (chi) has been evaluated. From EPR and optical studies it is observed that iron ions are present in trivalent state.</P>