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Mahuya Pakhira,Subhajit Ghosh,Surajit Ghosh,Dhruba P. Chatterjee,Arun K. Nandi 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.112 No.-
Biocidal and anti-fouling properties in the same polymeric membranes are important in biomedical fieldfor their capability to protect serious infection and filtration of biological molecules. For this purpose wereport random graft copolymer of poly(vinylidene fluoride) (PVDF-graft-poly(tBAEMA-ran-OEGMA,PVBO) membranes showing both antifouling and antibacterial activity. The PVBO copolymer is synthesisedusing ATRP technique and the polymer membranes are produced by breath figure (PVBO-1) andphase inversion (PVBO-2) techniques. The former shows well-developed hexagonal pores (diameter15 ± 3.5 nm), and good hydrophilicity (contact angle 41.3). PVBO-1 membrane exhibits good antifoulingproperty for both lysozyme and BSA solutions. Antimicrobial activity measured from agar diffusionmethod indicates that PVBO-1 shows a large zone of inhibition in case of gram-negative E coli andgram-positive S. aureus bacteria, whereas PVBO-2 and its HCl treated state (PVBO-3) membranes exhibitonly a moderate zone of inhibition. The MBC and MIC values indicate that the synthesized membranesare bactericidal in nature and not bacteriostatic. Live and dead cell assay indicates that PVBO-1 can significantlyinhibit growth of bacteria even at high bacteria concentration confirming high antimicrobialactivity. MTT assay suggests PVBO-1 is non-cytotoxic to mammalian cells and co-culturing activity resultsuggests high cellular selectivity of PVBO-1.
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