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Lipolysis Effect of Daucosterol Isolated from Mulberry (Morus alba) Leaves
Ke Li1(이커),Mi Lim Lee(이미림),Lu Que(루쿼),Mae Li(이매),Jum Soon Kang(강점순),Yung Hyun Choi(최영현),Kyung Mi Kim(김경미),Jae-Chul Jung(정재철),Dae Youn Hwang(황대연),Young Whan Choi(최영환) 한국생명과학회 2017 생명과학회지 Vol.27 No.12
뽕나무는 약 40여 속과 1,000여 종이 있는 것으로 알려져 있으며, 항염증, 항진정, 지사작용, 노화억제 및 신경보호작용이 있는 것으로 알려져 있다. 본 연구에서는 아시아 지역에서 전통 한약재로 사용되는 뽕 나무 잎의 지방분해 활성에 관여하고 있는 물질을 스크린하기 위하여 뽕잎 분말을 헥산, 에틸 아세테이트 및 메탄올로 순차 추출하였다. 뽕 잎의 EtOAc 추출물로부터 daucosterol이 순수분리 되었으며, 그 구조는 1H, 13C, DEPT, COSY, HSQC 및 HMBC 등의 NMR스펙트럼 분석에 의해 밝혀졌다. Daucosterol은 농도 의존적으로 지질분해 효과를 나타내었는데, 본 연구의 결과로부터 뽕나무 잎으로부터 순수분리한 daucosterol의 지분분해 활성은 다양한 질병을 치료하기 위한 천연물 소재 또는 지표성분으로서 활용이 가능할 것으로 생각된다. 그러나 보다 효율적으로 이용하기 위해서는 daucosterol의 비만에 관한 생리활성 기작에 대한 추가적인 연구가 필요할 것이다. Plants are reservoirs of naturally occurring chemical compounds and of structurally diverse bioactive molecules. The aim of this investigation was to screen for the presence of phytochemicals responsible for the lipolysis activity in mulberry (Morus alba) leaves, which are important in traditional Asian medicinal plants. Powdered mulberry leaves were extracted with hexane, ethyl acetate, and methanol. Daucosterol was isolated from the EtOAc extract of mulberry leaves, and its structure was elucidated by NMR spectral analyses. The NMR assignments for the compound were determined using 1H, 13C, DEPT, COSY, HSQC, and HMBC NMR spectral data. Daucosterol showed a concentration-dependent lipolysis activity that may impart medicinal properties that can be exploited by medical practitioners for the treatment of various diseases. However, further studies should be conducted to elucidate additional mechanisms of daucosterol.
Non-cracking YBCO Films on Silver Wire
( Dea Wha Soh ),( Korobova N. ),( Young Mae Li ) 한국동굴학회 2006 동굴 Vol.76 No.-
Electrophoretic deposition (EPD) of alcohol YBCO suspensions on the Ag wire electrode is studied. Poly(ethylene glycol) was coordinated to a structure formed by the EPD process with YBCO particles. The suspension is characterized in terms of zeta potential and conductivity. The d.c electric fields of 200-300 V/cm are applied for 1-10 min. The optimal condition for the EPD allows modifying the properties and microstructure of the deposited films. Superconducting coatings with nanometer-sized pores and a preferred orientation along the c-axis were prepared from the result with chemically modified precursor solution. In contrast, YBCO coatings of submicrometer-sized pores and randomly orientated grains were prepared from the solution without PEG
Evaluation of Bioactive Compounds in Strawberry Fruits by a Targeted Metabolomic Approach
Min Jung Ko,Ramesha H Jayaramaiah,Ravi Gupta,So Wun Kim,Jae Uk An,Ziyu Wang,Mae Li,Nam Jun Kang,Kwang-Pyo Hong,Jum-Soon Kang,Sun Tae Kim,Young Whan Choi 한국원예학회 2017 원예과학기술지 Vol.35 No.6
Strawberry (Fragaria ×ananassa Duch.) is referred to as a functional fruit because of its high nutritional value. Here, we used a targeted metabolomics approach to investigate the quantitative variation in strawberry bioactive compound concentration across cultivars and breeding lines and during fruit ripening. We also compared the efficiency of 95% ethyl alcohol (EtOH), 50% EtOH, and water in extracting these compounds. High-performance liquid chromatography (HPLC) and multivariate analysis revealed that 50% EtOH extracted bioactive compounds more efficiently than 95% EtOH and water. Screening of targeted bioactive compounds extracted with 50% EtOH in six cultivars and 28 breeding lines revealed high abundance of anthocyanins, flavonols, flavanols, and cinnamic acid, which constituted up to 6.4 mg·g<SUP>-1</SUP> of fresh weight of fruits. The highest concentration of phenolic compounds was observed in breeding lines ‘SB08’, ‘SB11’, ‘Arang’, ‘SB09’, and ‘SB10’. Furthermore, significant changes in the concentration of these compounds occurred during fruit ripening in four strawberry cultivars. Overall, our results highlight the chemotypic relationship between cultivars, breeding lines, and fruit ripening associated-changes in strawberries, and these findings provide a chemometric method for choosing optimal cultivars/breeding lines.