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Sung Yong Park,Young Mo Yoon,M. Wes Schilling,Koo Bok Chin 한국축산식품학회 2009 한국축산식품학회지 Vol.29 No.5
This study was conducted to investigate the effects of heating, fiber type used in solid-phase microextraction (SPME, two phase vs three phase) and storage time on the volatile compounds of porcine M. longissimus dorsi (LD). Volatile compounds were measured using a gas chromatography and mass spectrometry (GC/MS) with a quadrupole mass analyzer. Among the volatile compounds identified, aldehydes (49.33%), alcohols (24.63%) and ketones (9.85%) were higher in pre-heated loins (100oC/30 min), whereas, alcohols (34.33%), hydrocarbons (22.84%) and ketones (16.88%) were higher in non-heated loins. Heating of loins induced the formation of various volatile compounds such as aldehydes (hexanal) and alcohols. The total contents of hydrocarbons, alcohols, and carboxylic acids were higher in two phase fibers, whereas those of esters tended to be higher in three-phase fibers (p<0.05). Most volatile compounds increased (p<0.05) with increased storage time. Thus, the analysis of volatile compounds were affected by the fiber type, while heating and refrigerated storage of pork M. longissimus dorsi increased the volatile compounds derived from lipid oxidation and amino acid catabolism, respectively. This study was conducted to investigate the effects of heating, fiber type used in solid-phase microextraction (SPME, two phase vs three phase) and storage time on the volatile compounds of porcine M. longissimus dorsi (LD). Volatile compounds were measured using a gas chromatography and mass spectrometry (GC/MS) with a quadrupole mass analyzer. Among the volatile compounds identified, aldehydes (49.33%), alcohols (24.63%) and ketones (9.85%) were higher in pre-heated loins (100oC/30 min), whereas, alcohols (34.33%), hydrocarbons (22.84%) and ketones (16.88%) were higher in non-heated loins. Heating of loins induced the formation of various volatile compounds such as aldehydes (hexanal) and alcohols. The total contents of hydrocarbons, alcohols, and carboxylic acids were higher in two phase fibers, whereas those of esters tended to be higher in three-phase fibers (p<0.05). Most volatile compounds increased (p<0.05) with increased storage time. Thus, the analysis of volatile compounds were affected by the fiber type, while heating and refrigerated storage of pork M. longissimus dorsi increased the volatile compounds derived from lipid oxidation and amino acid catabolism, respectively.
Park, Sung-Yong,Yoon, Young-Mo,Schilling, M. Wes,Chin, Koo-Bok Korean Society for Food Science of Animal Resource 2009 한국축산식품학회지 Vol.29 No.5
This study was conducted to investigate the effects of heating, fiber type used in solid-phase microextraction (SPME, two phase vs three phase) and storage time on the volatile compounds of porcine M. longissimus dorsi (LD). Volatile compounds were measured using a gas chromatography and mass spectrometry (GC/MS) with a quadrupole mass analyzer. Among the volatile compounds identified, aldehydes (49.33%), alcohols (24.63%) and ketones (9.85%) were higher in pre-heated loins ($100^{\circ}C$/30 min), whereas, alcohols (34.33%), hydrocarbons (22.84%) and ketones (16.88%) were higher in non-heated loins. Heating of loins induced the formation of various volatile compounds such as aldehydes (hexanal) and alcohols. The total contents of hydrocarbons, alcohols, and carboxylic acids were higher in two phase fibers, whereas those of esters tended to be higher in three-phase fibers (p<0.05). Most volatile compounds increased (p<0.05) with increased storage time. Thus, the analysis of volatile compounds were affected by the fiber type, while heating and refrigerated storage of pork M. longissimus dorsi increased the volatile compounds derived from lipid oxidation and amino acid catabolism, respectively.
CCL2 Chemokine as a Potential Biomarker for Prostate Cancer: A Pilot Study
Igor Tsaur,Anika Noack,Jasmina Makarevic,Elsie Oppermann,Ana Maria Waaga-Gasser,Martin Gasser,Hendrik Borgmann,Tanja Huesch,Kilian M. Gust,Michael Reiter,David Schilling,Georg Bartsch,Axel Haferkamp,R 대한암학회 2015 Cancer Research and Treatment Vol.47 No.2
Purpose Prostate specific antigen is not reliable in diagnosing prostate cancer (PCa), making theidentification of novel, precise diagnostic biomarkers important. Since chemokines areassociated with more aggressive disease and poor prognosis in diverse malignancies, weaimed to investigate the diagnostic relevance of chemokines in PCa. Materials and MethodsPreoperative and early postoperative serum samples were obtained from 39 consecutivePCa patients undergoing radical prostatectomy. Serum from 15 healthy volunteers servedas controls. Concentrations of CXCL12, CXCL13, CX3CL1, CCL2, CCL5, and CCL20 weremeasured in serum by Luminex. The expression activity of CXCR3, CXCR4, CXCR5, CXCR7,CXCL12, CXCL13, CX3CR1, CXCL1, CCR2, CCR5, CCR6, CCR7, CCL2, and CCL5 mRNA wasassessed in tumor and adjacent normal tissue of prostatectomy specimens by quantitativereal-time polymerase chain reaction. The associations of these chemokines with clinicaland histological parameters were tested. ResultsThe gene expression activity of CCL2 and CCR6 was significantly higher in tumor tissuecompared to adjacent normal tissue. CCL2 was also significantly higher in the blood samplesof PCa patients, compared to controls. CCL5, CCL20, and CX3CL1 were lower in patientserum, compared to controls. CCR2 tissue mRNA was negatively correlated with the Gleasonscore and grading. ConclusionChemokines are significantly modified during tumorigenesis of PCa, and CCL2 is a promisingdiagnostic biomarker.