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Antibody-Mediated Resistance to Rhizomania Disease in Sugar Beet Hairy Roots
M,Jafarzade,M,Ramezani,F,Hedayati,Z,Mokhtarzade,B,Zare,M,S,Sabet,P,Norouzi,M,A,Malboobi 한국식물병리학회 2019 Plant Pathology Journal Vol.35 No.6
Agrobacterium rhizogenes–mediated transformation of sugar beet hairy roots expressing single-chain variable fragment (scFv) was exploited to evaluate the efficacy of four antibody-based constructs for interfering with the Beet necrotic yellow vein virus infection. The scFv specific to a major coat protein of virus, p21, was targeted to various cellular compartments including the cytosol (pIC and pICC constructs), apoplast (pIA), and mitochondrion (pIM). After mechanical virus inoculation, most of the hairy root clones expressing scFv in the cytosol displayed low virus titers while the majority of transgenic hairy root clones accumulated antibody in outer membrane of mitochondria or apoplast were infected. This hairy root system provided an efficient and rapid approach to initially investigating root disease resistance like rhizomania prior to transform whole recalcitrant plants such as sugar beet.
Antibody-Mediated Resistance to Rhizomania Disease in Sugar Beet Hairy Roots
M. Jafarzade,M. Ramezani,F. Hedayati,Z. Mokhtarzade,B. Zare,M. S. Sabet,P. Norouzi,M. A. Malboobi 한국식물병리학회 2019 Plant Pathology Journal Vol.35 No.6
Agrobacterium rhizogenes–mediated transformation of sugar beet hairy roots expressing single-chain variable fragment (scFv) was exploited to evaluate the efficacy of four antibody-based constructs for interfering with the Beet necrotic yellow vein virus infection. The scFv specific to a major coat protein of virus, p21, was targeted to various cellular compartments including the cytosol (pIC and pICC constructs), apoplast (pIA), and mitochondrion (pIM). After mechanical virus inoculation, most of the hairy root clones expressing scFv in the cytosol displayed low virus titers while the majority of transgenic hairy root clones accumulated antibody in outer membrane of mitochondria or apoplast were infected. This hairy root system provided an efficient and rapid approach to initially investigating root disease resistance like rhizomania prior to transform whole recalcitrant plants such as sugar beet.
Antibody-Mediated Resistance to Rhizomania Disease in Sugar Beet Hairy Roots
Jafarzade, M.,Ramezani, M.,Hedayati, F.,Mokhtarzade, Z.,Zare, B.,Sabet, M.S.,Norouzi, P.,Malboobi, M.A. The Korean Society of Plant Pathology 2019 Plant Pathology Journal Vol.35 No.6
Agrobacterium rhizogenes-mediated transformation of sugar beet hairy roots expressing single-chain variable fragment (scFv) was exploited to evaluate the efficacy of four antibody-based constructs for interfering with the Beet necrotic yellow vein virus infection. The scFv specific to a major coat protein of virus, p21, was targeted to various cellular compartments including the cytosol (pIC and pICC constructs), apoplast (pIA), and mitochondrion (pIM). After mechanical virus inoculation, most of the hairy root clones expressing scFv in the cytosol displayed low virus titers while the majority of transgenic hairy root clones accumulated antibody in outer membrane of mitochondria or apoplast were infected. This hairy root system provided an efficient and rapid approach to initially investigating root disease resistance like rhizomania prior to transform whole recalcitrant plants such as sugar beet.
A New Zero-Voltage-Switching Bridgeless PFC, Using an Active Clamp
Ramezani, Mehdi,Ghasedian, Ehsan,Madani, Seyed M. The Korean Institute of Power Electronics 2012 JOURNAL OF POWER ELECTRONICS Vol.12 No.5
This paper presents a new ZVS single phase bridgeless (Power Factor Correction) PFC, using an active clamp to achieve zero-voltage-switching for all main switches and diodes. Since the presented PFC uses a bridgeless rectifier, most of the time, only two semiconductor components are in the main current path, instead of three in conventional single-switch configurations. This property significantly reduces the conduction losses,. Moreover, zero voltage switching removes switching loss of all main switches and diodes. Also, auxiliary switch turns on zero current condition. The presented converter needs just a simple non-isolated gate drive circuitry to drive all switches. The eight stages of each switching period and the design considerations and a control strategy are explained. Finally, the converter operation is verified by simulation and experimental results.
A New Zero-Voltage-Switching Bridgeless PFC, Using an Active Clamp
Mehdi Ramezani,Ehsan Ghasedian,Seyed M. Madani 전력전자학회 2012 JOURNAL OF POWER ELECTRONICS Vol.12 No.5
This paper presents a new ZVS single phase bridgeless (Power Factor Correction) PFC, using an active clamp to achieve zero-voltage-switching for all main switches and diodes. Since the presented PFC uses a bridgeless rectifier, most of the time, only two semiconductor components are in the main current path, instead of three in conventional single-switch configurations. This property significantly reduces the conduction losses,. Moreover, zero voltage switching removes switching loss of all main switches and diodes. Also, auxiliary switch turns on zero current condition. The presented converter needs just a simple non-isolated gate drive circuitry to drive all switches. The eight stages of each switching period and the design considerations and a control strategy are explained. Finally, the converter operation is verified by simulation and experimental results.
Javad Sabzi,M. Reza Esfahani,Togay Ozbakkaloglu,Ahmadreza Ramezani 국제구조공학회 2023 Steel and Composite Structures, An International J Vol.46 No.1
The present study experimentally and analytically investigates the effect of tensile reinforcement ratio and arrangement on the behavior of FRP strengthened reinforced concrete (RC) beams. The experimental part of the program was comprised of 8 RC beams that were tested under four-point bending. Results have shown that by keeping the total cross-section area of tensile reinforcing bars constant, in specimens with a low reinforcement ratio, increasing the number and decreasing the diameter of bars in the section lead to 21% and 29% increase in the load-carrying capacity of specimens made with normal and high compressive strength, respectively. In specimens with high reinforcement ratio, a different behavior was observed. Furthermore, the accuracy of the existing code provisions and analytical models in predicting the load-carrying capacity of the FRP strengthened beams failed by premature debonding mode were evaluated. Herein, a model is proposed which considers the tensile reinforcement ratio (as opposed to code provisions) to achieve more accurate results for calculating the load carrying capacity of FRP strengthened RC beams.
New bound on MIS and MIN-CDS for a unit ball graph
D.A. Mojdeh,M. Ghanbari,M. Ramezani 한국통신학회 2017 ICT Express Vol.3 No.3
The size of the maximum independent set (MIS) in a graph G is called the independence number. The size of the minimum connected dominating set (MIN-CDS) in G is called the connected domination number. The aim of this paper is to determine two better upper bounds of the independence number; dependent on the connected domination number for a unit ball graph. Further, we improve the upper bound to obtain the best bound with respect to the upper bounds obtained thus far.