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- 저자 : Luke Lee (검색결과 54 건)
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Lee, Jae Young,Park, Younggeun,Pun, San,Lee, Sung Sik,Lo, Joe F.,Lee, Luke P. The Royal Society of Chemistry 2015 Nanoscale Vol.7 No.23
<P>Intracellular Cyt c release profiles in living human neuroblastoma undergoing amyloid β oligomer (AβO)-induced apoptosis, as a model Alzheimer's disease-associated pathogenic molecule, were analysed in a real-time manner using plasmon resonance energy transfer (PRET)-based spectroscopy.</P>
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Bubble-free rapid microfluidic PCR
Lee, Sang Hun,Song, Jihwan,Cho, Byungrae,Hong, SoonGweon,Hoxha, Ori,Kang, Taewook,Kim, Dongchoul,Lee, Luke P. Elsevier 2019 Biosensors & Bioelectronics Vol.126 No.-
<P><B>Abstract</B></P> <P>Microfluidic polymerase chain reaction (PCR) has been of great interest owing to its ability to perform rapid and specific nucleic acid amplification and analysis on small volumes of samples. One of the major drawbacks of microfluidic PCR is bubble generation and reagent evaporation, which can cause malfunctions. Here, through theoretical modeling and characterization of bubble behavior, we propose a bubble-free microfluidic PCR device via controlled fluid transfer. Our approach exploits a thin impermeable polyethylene (PE) top layer that minimizes the generation of bubbles by inhibiting mass transport along a vertical direction. Simulation results demonstrate that a calculated mass flow difference of approximately 370% can be obtained by utilizing an impermeable membrane as the vertical barrier layer. To demonstrate proof-of-concept, two nanoporous polymeric materials, poly(dimethylsiloxane) (PDMS) and PE, were used for stand-alone self-powered sample loading (approximately 70 s) and for use as a vertical barrier layer, respectively. Consequently, we demonstrate successful amplification of the cMET gene, a nucleic acid (NA) biomarker for lung cancer, and complete an ultrafast PCR test in less than 3 min using a high powered Peltier-based thermal cycler under bubble-free conditions. This approach will result in a new paradigm for ultrafast molecular diagnosis and can facilitate NA-based nearly instantaneous diagnostics for point-of-care testing and for personalized and preventive medicine.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The major drawbacks of microfluidic PCR is bubble generation and reagent evaporation. </LI> <LI> A bubble-free microfluidic PCR device via controlled fluid transfer through theoretical modeling and characterization of bubble behavior. </LI> <LI> An impermeable polyethylene layer minimizes the generation of bubbles by inhibiting mass transport along a vertical direction. </LI> <LI> The microfluidic PCR chamber design with surrounding circumferential chamber for a guided-fluid transport of generated bubble. </LI> <LI> Successful nucleic acid amplification of the cMET gene, and an ultrafast PCR test in less than 3 min. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
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Whang, Keumrai,Lee, Jong-Hwan,Shin, Yonghee,Lee, Wooju,Kim, Young Wan,Kim, Dongchoul,Lee, Luke P.,Kang, Taewook Nature Publishing Group UK 2018 Light, science & applications Vol.7 No.1
<▼1><P>A rapid, precise method for identifying waterborne pathogens is critically needed for effective disinfection and better treatment. However, conventional methods, such as culture-based counting, generally suffer from slow detection times and low sensitivities. Here, we developed a rapid detection method for tracing waterborne pathogens by an innovative optofluidic platform, a plasmonic bacteria on a nanoporous mirror, that allows effective hydrodynamic cell trapping, enrichment of pathogens, and optical signal amplifications. We designed and simulated the integrated optofluidic platform to maximize the enrichment of the bacteria and to align bacteria on the nanopores and plasmonic mirror via hydrodynamic cell trapping. Gold nanoparticles are self-assembled to form antenna arrays on the surface of bacteria, such as <I>Escherichia coli</I> and <I>Pseudomonas aeruginosa</I>, by replacing citrate with hydroxylamine hydrochloride in order to amplify the signal of the plasmonic optical array. Owing to the synergistic contributions of focused light via the nanopore geometry, self-assembled nanoplasmonic optical antennas on the surface of bacteria, and plasmonic mirror, we obtain a sensitivity of detecting <I>E. coli</I> as low as 10<SUP>2</SUP> cells/ml via surface-enhanced Raman spectroscopy. We believe that our label-free strategy via an integrated optofluidic platform will pave the way for the rapid, precise identification of various pathogens.</P></▼1><▼2><P><B>Nanoparticles: making it easier to detect waterborne pathogens</B></P><P>A novel method using nanotechnology to rapidly identify bacteria in water samples has been developed by scientists in the Republic of Korea and the US. Waterborne pathogens kill millions of people each year, but detection methods can be time-consuming, lack sensitivity, and only work with small samples. Taewook Kang at Sogang University, Seoul, and co-workers fabricated a gold-coated mirror filled with nano-sized holes smaller than an individual bacterium, and added especially modified gold nanoparticles to their water samples. The nanoparticles stuck onto the surface of bacteria, such as <I>Escherichia coli</I>, which in turn made the bacteria likely to cluster around the mirror’s pores. These measures greatly enhanced the interactions between the bacteria and light, so that even very low cell concentrations could be detected using spectroscopy, and different species of bacteria could be distinguished.</P></▼2>
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( Tae Hee Lee ),( Jiyoon Bu ),( Sung Hee Hyun ),( Woo Sun Rou ),( Seok Hyun Kim ),( Byung Seok Lee ),( Luke J. Kubiatowicz ),( Seungpyo Hong ),( Hyuk Soo Eun ) 대한간학회 2020 춘·추계 학술대회 (KASL) Vol.2020 No.1
Aims: Hepatocellular carcinoma (HCC) is frequently diagnosed at the advanced stage, delaying the timely treatment. Therefore, the early detection of HCC can reduce the mortality of a cancer by inhibiting its progression in earlier stage. Herein, we designed a non-invasive, low cost liquid biopsy platform based on the analysis of circulating cell-free DNA (cfDNA) for the early diagnosis of HCC. cfDNA is extracted by the polydopamine (PDA)-silica (SiO<sub>2</sub>) hybrids, which synergistically interact with different part of nucleotide, allowing more accurate sensing of cfDNA. We evaluated the diagnostic capability of our cfDNA testing platform for detecting early stage HCC. Methods: PDA-SiO<sub>2</sub> hybrids were coated on the alginate beads, in order to enhance the cfDNA adsorption. The beads were directly applied on human plasma samples after treating with proteinase K and lysis buffer. Plasma cfDNA concentrations were quantified using NanoDrop 1000 (Thermo Scientific). Total 209 individuals were enrolled in this study including 99 patients with HCC, 50 patients with liver cirrhosis, 30 patients with alcoholic liver disease, and 30 healthy individuals, respectively. Results: Average cfDNA concentration was significantly higher for HCC patients compared to both healthy donors and other types of liver disease patients, including alcoholic liver disease and liver cirrhosis (1.68 ± 1.2 ng/μL vs. 0.16 ± 0.17 ng/μL, 0.41 ± 0.28 ng/μL, and 0.59 ± 0.49 ng/μL, respectively; P<0.0001). Other serum biomarkers such as ALT, AST, ALP, total protein level, and platelet concentration did not show significant difference between the cancer patients and at least one of the control groups. Obviously, alpha-fetoprotein (AFP) was the only serum protein that demonstrated high diagnostic capability for HCC patients, exhibiting 655.2 ± 3,181.3 ng/mL, 9.7 ± 19.9 ng/mL, 11.4 ± 32.4 ng/mL, and 3.4 ± 1.9 ng/mL for the patients with cancer, alcoholic liver disease, liver cirrhosis, and non-diseased, respectively (P<0.0001). We further conducted receiver operating characteristic (ROC) curve analysis and confirmed that cfDNA concentration has superior diagnostic capability by showing high level of area under the ROC curve (AUC-ROC) (0.896, 95% CI: 0.855-0.937; P<0.0001) than that of serum AFP level (0.770, 95% CI: 0.705-0.835; P<0.0001). Interestingly, when combining these two markers together by adding normalized serum AFP levels and plasma cfDNA concentrations, the AUC-ROC value has increased up to 0.907 (95% CI: 0.867-0.947; P<0.0001), showing the best HCC diagnostic ability. Conclusions: We have demonstrated that cfDNA has higher diagnostic functionality to differentiate patients with HCC from other types of liver diseases, compared to other conventional serum biomarkers. When analyzed together with AFP, the diagnostic capability could be further enhanced, showing both higher sensitivity and specificity than either of cfDNA or serum AFP. Therefore, our cfDNA-based diagnosis platform enables patients to receive appropriate curative therapy at the earlier stage of the tumor.
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LEE, Won-Jun,SHIN, Luke Yunkeun Korea Distribution Science Association 2022 유통과학연구 Vol.20 No.11
Purpose: The wide distribution of smartphones has changed life and user behavior. This phenomenon has both advantages and disadvantages for users. As smartphones become a part of our daily lives, smartphone addiction has recently become a social issue in many countries. This study explores factors that affect smartphone addiction and the consequences of addictive behaviors. Research design, data and methodology: Our model hypothesizes that four key factors determine addictive behavior: flow, enjoyment, preference for online social life, and escape reality. Commitment and compulsive use are mediating variables that connect key drivers and addictive results. Based on the SEM (structural equation model) analysis of 497 survey responses, these four driving factors each have a significant effect on the compulsive use of smartphones directly or indirectly; the compulsive use of smartphones directly influences the three results Results: We conducted a reliability and validity analysis, and the results were successful. In the hypothesis test, every path is accepted as expected at the significance level of 0.05. Conclusions: Among the four driving factors, escape reality is the vital factor influencing smartphone addiction and its consequences. And anxiety is the number one consequence influenced by the compulsive use of smartphones.
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