Optimization of the Experimental Parameters Affecting the Corrosion Behavior for Mg–Y–Zn–Mn Alloy via Response Surface Methodology

Lisha Wang,Jinghua Jiang,Bassiouny Saleh,Reham Fathi,He Huang,Huan Liu,Aibin Ma 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.12

This paper optimizes experimental parameters on controlling corrosion performance of the Mg-rare-earth alloys with longperiod stacking ordered structure using response surface methodology. Different NaCl concentrations, temperatures, andvarying pH values are selected as input parameters. The corrosion current density (icorr) and open circuit potential (OCP)are used as the multiple responses to evaluate the corrosion performances. The corrosion surface morphology and corrosionmechanism of the Mg alloys are also investigated by scanning electron microscopy for the optimization. Analysis of variance(ANOVA) has given the impact of individual factors and interactions on the corrosion rate. The results indicated thatthe three test parameters had significant impacts in controlling the corrosion behavior of Mg alloy. Moreover, the increasedNaCl concentration decreased the pitting potential (Epit) of the target materials. Filiform corrosion can be detected in high pHsolutions, whereas the matrix suffered from severe dissolution phenomenon in low pH solutions. High temperature aggravatedthe local destruction and dissolution of the protective film. The interaction of the experimental parameters showed a sizableinfluence on corrosion performance.

Structural reliability updating using experimental data

Lisha Zhu,Xianzhen Huang,Cong Yuan,Zunling Du 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.1

Conventional reliability analysis requires the information from an existing structure, such as a mechanical model and random distributed inputs. In many engineering problems, a state monitoring system commonly provides experimental or monitoring data, which can be used to update the initial estimation for structural reliability to reduce prediction uncertainty. A critical issue in this process is the manner in which the existing information and new data can be reasonably integrated into a reliability estimation. In this paper, Bayesian updating approach is applied to incorporate the additional data. Firstly, a theoretical model is established to predict the prior distribution of the limit state function (LSF) with the first-order reliability method. Then, the Bayesian inference theory is applied to update the probability distribution parameters of LSF using the acquired experimental or monitoring data. The analytical form of the LSF’s posterior distribution is derived under the assumption that the experimental test error follows a normal distribution. To improve accuracy, a second-order reliability method is proposed based on the theory of saddlepoint approximation. Markov chain Monte Carlo simulation is used to derive a general method for updating the LSF’s distribution using the experimental or monitoring data. Finally, three numerical examples are provided to illustrate the proposed framework’s validity.

The Mitogen-Activated Protein Kinase Signal Transduction Pathways in Alternaria Species

Houjuan Xu,Xiaoxue Xu,Yujun Wang,비벡바지파이,Lisha Huang,Yongfang Chen,백광현 한국식물병리학회 2012 Plant Pathology Journal Vol.28 No.3

Mitogen-activated protein kinase (MAPK) cascades are conserved signaling modules in the eukaryotic cells. They are involved in many major cell processes in fungi such as stress responses, vegetative growth, pathogenicity,secondary metabolism and cell wall integrity. In this review, we summarized the advances of research on the MAPK signaling pathways in Alternaria species. As major phytopathogenic fungi, Alternaria species reduce crop production. In contrast to the five MAPK pathways known in yeast, only three MAPK pathways as Fus3/Kss1-type, Hog1-type, and Slt2-type have been characterized in Alternaria. The Fus3/Kss1-type MAPK pathway participates in regulation of vegetative growth,conidiation, production of some cell-wall-degrading enzymes and pathogenicity. The Hog1-type pathway is involved in osmotic and oxidative stress, fungicides susceptibility and pathogenicity. The Slt2-type MAP kinases play an important role on maintaining cell wall integrity, pathogenicity and conidiation. Although recent advances on the MAPK pathways in Alternaria spp. reveal many important features on the pathogenicity,there are many unsolved problems regarding to the unknown MAP kinase cascade components and network among other major signal transduction. Considering the economic loss induced by Alternaria spp., more researches on the MAPK pathways will need to control the Alternaria diseases.

Transcriptional analysis reveals formation of axillary solitary hook in vine plant Uncaria rhynchophylla

Wan Lingyun,Pan Limei,Song Lisha,Fu Jine,Huang Qiulan,Ji Xiaowen,Wei Shugen,Zhang Zhanjiang 한국식물생명공학회 2023 Plant biotechnology reports Vol.17 No.5

The hook of Uncaria rhynchophylla is an important external phenotype representing the quality of Gou-Teng. The hook formation pattern of U. rhynchophylla displays a unique feature that solitary hook and opposite hooks alternately occur in the leaf axils forming a liner series. The solitary hook only grows on the lower side of the leaf axil, resulting from the decline in the total yield of hooks. However, the reasons for solitary hook formation in U. rhynchophylla are not clear. Therefore, a survey on the molecular mechanisms of solitary hook formation needs to be performed urgently. We obtained 250.67 million high-quality clean reads from the lower side of axillary stem segments (HS) and the upper side of axillary stem segments (HSCK) libraries. We detected 42 DEGs (differentially expressed genes) between HS and HSCK libraries; 11 were down-regulated, and 31 were up-regulated. Gene ontology functional classification of the DEGs indicated that they included two genes that encoded LAZY1 related to the regulation of polar auxin transport, two genes that encoded AUX/IAA and AP2/ERF related to plant hormone signaling transduction, and two genes that encoded F3H related to the flavone synthase activity. These six genes were associated with lateral polar auxin transport (PAT) regulation, and their expression level had a high correlation with the pattern of IAA distribution upon gravity stimulation. And the inhibition of solitary hook development was observed after treatment with N-1-naphthylphthalamic acid (NPA) or quercetin, which was known as the polar auxin inhibitor. The above findings might provide new information about the molecular mechanisms of regulation of lateral PAT in the axillary bud development process under gravity.

The Mitogen-Activated Protein Kinase Signal Transduction Pathways in Alternaria Species

Xu, Houjuan,Xu, Xiaoxue,Wang, Yu-Jun,Bajpai, Vivek K.,Huang, Lisha,Chen, Yongfang,Baek, Kwang-Hyun The Korean Society of Plant Pathology 2012 Plant Pathology Journal Vol.28 No.3

Mitogen-activated protein kinase (MAPK) cascades are conserved signaling modules in the eukaryotic cells. They are involved in many major cell processes in fungi such as stress responses, vegetative growth, pathogenicity, secondary metabolism and cell wall integrity. In this review, we summarized the advances of research on the MAPK signaling pathways in Alternaria species. As major phytopathogenic fungi, Alternaria species reduce crop production. In contrast to the five MAPK pathways known in yeast, only three MAPK pathways as Fus3/Kss1-type, Hog1-type, and Slt2-type have been characterized in Alternaria. The Fus3/Kss1-type MAPK pathway participates in regulation of vegetative growth, conidiation, production of some cell-wall-degrading enzymes and pathogenicity. The Hog1-type pathway is involved in osmotic and oxidative stress, fungicides susceptibility and pathogenicity. The Slt2-type MAP kinases play an important role on maintaining cell wall integrity, pathogenicity and conidiation. Although recent advances on the MAPK pathways in Alternaria spp. reveal many important features on the pathogenicity, there are many unsolved problems regarding to the unknown MAP kinase cascade components and network among other major signal transduction. Considering the economic loss induced by Alternaria spp., more researches on the MAPK pathways will need to control the Alternaria diseases.