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The Antioxidant Properties of Garlic Compounds: Allyl Cysteine, Alliin, Allicin, and Allyl Disulfide
Lip Yong Chung 한국식품영양과학회 2006 Journal of medicinal food Vol.9 No.2
Garlic and garlic extracts, through their antioxidant activities, have been reported to provide protection againstfree radical damage in the body. This study investigated antioxidant properties of garlic compounds representing the four mainchemical classes, alliin, allyl cysteine, allyl disulfide, and allicin, prepared by chemical synthesis or purification. Alliin scav-enged superoxide, while allyl cysteine and allyl disulfide did not react with superoxide. Allicin suppressed the formation ofsuperoxide by the xanthine/xanthine oxidase system, probably via a thiol exchange mechanism. Alliin, allyl cysteine, and al-lyl disulfide all scavenged hydroxyl radicals; the rate constants calculated based on deoxyribose competitive assay were1.41.7. 1010, 2.12.2. 109, and 0.71.5. 1010 M. 1 second. 1, respectively. Contrary to previous reports, allicin did notexhibit hydroxyl radical scavenging activity in this study. Alliin, allicin, and allyl cysteine did not prevent induced microso-mal lipid peroxidation, but both alliin and allyl cysteine were hydroxyl scavengers, and allyl disulfide was a lipid peroxida-tion terminator. In summary, our findings indicated that allyl disulfide, alliin, allicin, and allyl cysteine exhibit different pat-terns of antioxidant activities as protective compounds against free radical damage.
중합효소 연쇄반응 ( Polymerase Chain Reaction ) 에 의한 단순포진 바이러스의 분류
김용현 ( Yong Hyun Kim ),전재복 ( Jae Bok Jun ),김도원 ( Do Won Kim ),정상립 ( Sang Lip Chung ),김정철 ( Jung Chul Kim ) 대한피부과학회 1996 대한피부과학회지 Vol.34 No.2
Background: Herpes simplex virus(HSV) infections are very common viral illnesses in dermatology and they have shown a tendency to increase in prevalence and incidence, and they would seem to have become more prevalent recently. This has resulted in an increased need for the rapid diagnosis of these infections. It has also become important to recognize the types of HSV in the clinical setting, because the two types differ in their natural histories and prognoses. Objective : The purpose of this study is to detect and type HSV DNA by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP). We investigated the relationship between the clinical manifestations and type using PCR. Methods : The study population consisted of 40 cases of HSV infections and 10 cases of varicella-zoster virus infections as negative controls. We used a pair of primers designated by Sakaoka et. al and performed restriction analysis after PCR. Results : No specific amplification was observed using the varicella-zoster virus. A total of 40 patients were examined by PCR and 28 were positive. Of 17 patients with lesions which developed above the umbilicus, l0 were positive. Out of 8 patients with lesions below the umbilicus, 5 were positive. Virus genomes were detected in the 13 out of 15 patients with eczema herpeticum. Conclusion : The method used in this study is useful in differentiating HSV-1 from HSV-2 in fections. These data suggest that the PCR results were nearly consistent with the clinical manifestations. (Kor J Dermatol 1996;34(2): 248-256)
김용현 ( Yong Hyun Kim ),백승혜 ( Seung Hye Paek ),전재복 ( Jae Bok Jun ),정상립 ( Sang Lip Chung ) 대한피부과학회 1996 대한피부과학회지 Vol.34 No.5
We report a case of cutaneous horn originated frorn condyloma acuminata in a 25 year-old man. The patient showed yellowish brown hyperkeratotic masses on the prepuce, coronal sulcus and multiple asymptomatic pin'kish cauliflower-like projections in both inguinal folds and perianal area. Skin biopsies were taken from the base of the penile lesions andinguinal, perianal area exhibited marked hyperkeratosis and parakeratosis in addition to the typical findings of condylorna acuminaturn. In situ hybridization technique for Human papilloma virus(HPV) showed positive reaction to HPV 11. All lesions were removed by surgical excision, electrocauterization and podophyllin application, but condyloma acuminatum recurred several times thereafter. (Kor J Dermatol 1996;34(5): 851-855)
김동립(Dong Lip Kim),정현철(Hyung Cheol Chung),노재경(Jae Kyung Roh),최진혁(Jin Hyuk Choi),박용준(Yong Jun Park),이화영(Wha Young Lee),김원호(Won Ho Kim),장준(Joon Chang),이상인(Sang In Lee),김승민(Seung Min Kim),서창옥(Chang Ok Suh 대한내과학회 1989 대한내과학회지 Vol.37 No.6
N/A We reviewed 10 cases of leptomentingeal car cinomatosis seen at the Yonsei Medical Center and Yonsei Cancer Canter from August 1987 to April 1989. The primary diseases of meningeal metastasis were stomach cancer (5 cases), lung cancer (3 cases) and breast cancer (1 cases). In one case, the primary site was not identified. Signs and symptoms were classified as involving the brain, cranial nerves, or spinal nerves. Most patients had symptoms or signs in more than one area. On initial spinal fluid examination of 7 patients, 4 patients had cytologic evidence of leptomeningeal metastasis. Repested spinal fluid assay yielded a positive cytology in anjother 2 patients. Eight patients were treated by intrathecal methotrexate administration and/or brain radiotherapy. Five of them experienced improvement or stabilization of neurologic sympoms; median survival was 40 days (27~45 days) after diagnosis. Median survival of the remaining 3 nonresponders was 17 days (15~18 days) after diagnosis. We concluded that active treatment of leptomentingeal carcinomatosis with intrathecal methotrexate and brain irradiation may increase the quality of life, and further-more, may prolong survival in some patients.
형광 편광 면역측정법을 이용한 Diffusion Chamber에서의 경피 흡수 측정
이원주 ( Weon Ju Lee ),김용현 ( Yong Hyun Kim ),김도원 ( Do Won Kim ),전재복 ( Jae Bok Jun ),정상립 ( Sang Lip Chung ) 대한피부과학회 1996 대한피부과학회지 Vol.34 No.5
Background: The penetraton in vivo of topically applied substances can be assessed by physiological or pharmacalogical signs or analysed by chemical or histological techniques. In vitro absorption can be commonly quantitated by measuring the passage of a radioisotope-labelled substance across skin that has been mounted in a diffusion chamber. Objective : Fluorescence polarization immunoassay technique has made the possible rapid growth of therapeutic drug nonitoring. We applied this methodology in measuring percutaneous absorption in a diffusion chamber. Methods : We utilized sheets of whole epidermis prepared from the circumcised prepuce. Some epidermal sheets were treated with 2 ml of acetone for 2 minutes, and others not. The epidermal sheet was mounted in a diffusion chamber between the donor compartment for the penetrant and the receptor compartment containing saline. Lidocaine HC1(10 microgram/cm) in vehicle(propylene glycol:ethanol; 7:3vol/vol) was applied to the donor compartment for the penetrant. With flow rate of about 3 ml/h all of the receptor phase collected during 2 hours interval were quantitated for 10 hours by the fluorescence polarization immunoassay. Results : Total absorption of lidocaine HC1 in the acetone-untreated group was 2.14-0.74% of the applied dose. Total absorption in the acetone-treated group showed no substantial difference (2.09+1.25%) compared to those of acetone-untreated group. The amount of lipid extracted from a epiderrnal sheet with acetone was 19+2.97%. Conclusion : Fluorescence polarization immunoassay may be a useful method in measuring percutaneous absorption in vitro. (Kor J Dermatol 1996;34(5): 718-724)
Kim, Do Won,Jun, Jae Bok,Kim, Yong Hyun,Paek, Seung Hye,Chung, Sang Lip 대한피부과학회 1996 Annals of Dermatology Vol.8 No.1
Subepidermal calcified nodule(SCN) is a form of calcinosis cubs which is usually present as a single small, raised, hard nodule with verrucous surface. A 13-year-old girl presented with a 10-month history of a ricegrain-sized, hard, yellowish white papule on both upper eyelids. The lesions were asymptomatic and had increased slowly in size. An excisional biopsy specimen of the left upper eyelid showed acanthosis and narrow pointed rete ridges of the epidermis, and closely aggregated deposition of basophilic material in the uppermost dermis. The material in the dermis did not stain with von Kossa. It was confirmed as calcium deposition by staining with alizarin red S which is far more specific for calcium than the von Kossa stain.