Health oil preparation from gardenia seeds by aqueous enzymatic extraction combined with puffing pre-treatment and its properties analysis

Chengyu Jin,Lingyun Wang,Xiaoying Liu,Yuanchao Lu,Ningxiang Yu,Xiaohua Nie,Qin Ye,Xianghe Meng 한국식품과학회 2023 Food Science and Biotechnology Vol.32 No.14

Gardenia jasminoides Ellis, a representative for “homology of medicine and food”, can be used to produce pigment and edible oil. Here, aqueous enzymatic extraction (AEE) combined with puffing pre-treatment was explored to prepare oil from gardenia seeds. Both wet-heating puffing (WP) at 90 °C and dry-heating puffing (DP) at 1.0 MPa facilitated the release of free oil by AEE, resulting in the highest free oil yields (FOY) of 21.8% and 23.2% within 3 h, much higher than that of un-puffed group. Additionally, active crocin and geniposide were also completely released. The FOY obtained was much higher than mechanical pressing method (10.44%), and close to solvent extraction (25.45%). Microstructure analysis indicated that gardenia seeds expanded by dry-heating puffing (1.0 MPa) had a larger, rougher surface and porous structure than other groups. Overall, AEE coupled with puffing pre-treatment developed is an eco-friendly extraction technology with high efficiency that can be employed to oil preparation.

Measurement of small co-seismic deformation field from multi-temporal SAR interferometry: application to the 19 September 2004 Huntoon Valley earthquake

Lee, Won-Jin,Lu, Zhong,Jung, Hyung-Sup,Ji, Lingyun Informa UK (TaylorFrancis) 2017 Geomatics, natural hazards and risk Vol.8 No.2

<P>Interferometric synthetic aperture (InSAR) has been widely applied to natural disaster monitoring. However, it has limitations due to the influence of noise sources such as atmospheric and topographic artefacts, data processing errors, etc. In particular, atmospheric effect is one of the most prominent noise sources in InSAR for the monitoring of small magnitude deformations. In this paper, we proposed an efficient multitemporal InSAR (MTInSAR) approach to measure small co-seismic deformations by minimizing atmospheric anomalies. This approach was applied to investigate the 18 September 2004 earthquake over Huntoon Valley, California, using 13 ascending and 22 descending ENVISAT synthetic aperture radar (SAR) images. The results showed that the co-seismic deformation was +/- 1.5 and +/- 1.0 cm in the horizontal and vertical directions, respectively. The earthquake source parameters were estimated using an elastic dislocation source from the ascending and descending acquisitions. The root mean square errors between the observed and modelled deformations were improved by the proposed MTInSAR approach to about 3.8 and 1.8 mm from about 4.0 and 5.2 mm in the ascending and descending orbits, respectively. It means that the MTInSAR approach presented herein remarkably improved the measurement performance of a small co-seismic deformation.</P>

Fluorination of dichlorodifluoromethane to synthesize tetrafluoromethane over Cr_2O_3–AlF_3 catalyst

Wenzhi Jia,Mengfei Luo,Lingyun Jin,Yuejuan Wang,Jiqing Lu 한국공업화학회 2011 Journal of Industrial and Engineering Chemistry Vol.17 No.3

A series of Cr_2O_3–AlF_3 catalysts with different Cr_2O_3 contents were prepared by a deposition–precipitation method for the fluorination of dichlorodifluoromethane (CCl_2F_2) to synthesize tetrafluoromethane (CF4). The highest activity was obtained on the Cr_2O_3–AlF_3 catalyst containing 61.2 wt.% of Cr_2O_3, with the CF_4 yield of 94% at 400℃. X-ray diffraction (XRD) and Raman spectroscopy results indicated the presence of bulk Cr_2O_3 and a-AlF_3 phases in the catalysts. Ammonia temperature programmed desorption (NH3-TPD) and Fourier transformation infrared spectroscopy (FTIR) of pyridine chemisorption revealed strong Lewis acidity of the catalysts. The Cr species and Lewis acid in the Cr_2O_3–AlF_3 catalysts were the active sites of fluorination and dismutation reaction of CCl_2F_2, respectively. This indicated bi-function catalytic performance of Cr_2O_3–AlF_3 catalysts for fluorination and dismutation reaction.

Characteristics and significance of heterogeneity of sea−land transitional facies shale gas reservoir in North Guizhou, China

Ran Wang,Shuxun Sang,Jun Jin,Lingyun Zhao,Wei Gao,Wei Fu,Fulun Shi,Ende Deng 한국지질과학협의회 2019 Geosciences Journal Vol.23 No.1

In order to identify the characteristics of the longitudinal heterogeneity of the sea–land transitional facies shale gas reservoir in the upper Yangtze region of North Guizhou, studies on the lithological combination, rock and mineral composition, geochemical parameters and reservoir microanisotropy characteristics of Longtan Formation in the study area are conducted on the basis of core observation, testing of geochemistry and reservoir physical property and well logging interpretation. The studies show that the lithological assemblages of the Longtan Formation are diverse and form an amina interbedding of “sand-mud-coal” with obvious cyclicity characteristics. There is a large longitudinal difference in rock and mineral composition and the average mass fraction of the clay mineral is 39.83%, which is obviously higher than that of the marine shale in North America and South China; the longitudinal heterogeneity of the organic matter abundance is high, with an average of 2.17% in the upper part, and 4.51% in the lower part; in accordance with the results observed with the scanning electron microscope and results calculated through pore fractal, the microscopic pore heterogeneity of the reservoir is high. The comparison and analysis of connecting wells with different scales in the study area show that the control effect of the depositional environment on longitudinal macroscopic heterogeneity of Longtan Formation is obvious, and the longitudinal microscopic heterogeneity is controlled through diagenesis. Meanwhile, studies with main coal mining seam as the seam section division method conclude that the heterogeneity of Coal Seam Sections 4 to 5 and Coal Seam Sections 13 to 15 is significantly smaller than that in other seam sections, and the Coal Seam Sections 4 to 5 and Coal Seam Sections 13 to 15 can be considered as a priority key seam section during development of shale gas.

TFPI inhibits breast cancer progression by suppressing ERK/p38 MAPK signaling pathway

Xing Mengying,Yang Ying,Huang Jiaxue,Fang Yaqun,Jin Yucui,Li Lingyun,Chen Xiang,Zhu Xiaoxia,Ma Changyan 한국유전학회 2022 Genes & Genomics Vol.44 No.7

Background: Tissue factor pathway inhibitor-1 (TFPI) is a serine protease inhibitor, which is responsible for inactivating TF-induced coagulation. Recently, increasing studies revealed that TFPI was lowly expressed in tumor cells and exhibited the antitumor activity. Objective: The aim of this study was to explore the role and underlying molecular mechanisms of TFPI in breast cancer. Methods: The expression and prognostic value of TFPI were analyzed using UALCAN and Kaplan-Meier plotter website. The expression level of TFPI in breast cancer tissues and cells was examined by immunohistochemistry (IHC) and western blot analysis, respectively. Cellular proliferation was evaluated by CCK-8 and colony formation assays. Cell migration and invasion were determined by transwell assay. The methylation level of TFPI promoter was determined by methylation-specific PCR. Results: TFPI expression was significantly lower in breast cancer tissues and cells compared to normal breast tissues and normal breast cells. Patients with low TFPI levels showed worse overall survival (OS). Furthermore, overexpression of TFPI significantly inhibited the proliferation, migration and invasion of breast cancer cells. Conversely, knockdown of TFPI promoted the proliferation, migration and invasion of breast cancer cells. Mechanistically, TFPI inhibited the ERK/p38 MAPK signaling pathway in breast cancer. Moreover, DNA hypermethylation of TFPI promoter was responsible for the downregulation of TFPI in breast cancer cells. Conclusion: TFPI inhibited breast cancer cell proliferation, migration and invasion through inhibition of the ERK/p38 MAPK signaling pathway, suggesting that TFPI may serve as a novel prognostic biomarker and therapeutic target for breast cancer.

m6A-mediated upregulation of AC008 promotes osteoarthritis progression through the miR-328-3p‒AQP1/ANKH axis

Yang Jiashu,Zhang Ming,Yang Dawei,Ma Yunfei,Tang Yuting,Xing Mengying,Li Lingyun,Chen Li,Jin Yucui,Ma Changyan 생화학분자생물학회 2021 Experimental and molecular medicine Vol.53 No.-

Long noncoding RNAs (lncRNAs) have emerged as important regulators of osteoarthritis (OA), but the biological roles and clinical significance of most lncRNAs in OA are not fully understood. Microarray analysis was performed to identify differentially expressed lncRNAs, mRNAs, and miRNAs between normal and osteoarthritic cartilage. We found that AC008440.5 (abbreviated AC008), as well as AQP1 and ANKH, were highly expressed in osteoarthritic cartilage, whereas miR-328-3p was expressed at a low level in osteoarthritic cartilage. Functional assays showed that ectopic expression of AC008, AQP1, and ANKH significantly decreased chondrocyte viability and promoted chondrocyte apoptosis and extracellular matrix (ECM) degradation, whereas knockdown of AC008, AQP1, and ANKH resulted in the opposite effects. Moreover, miR-328-3p overexpression increased chondrocyte viability and attenuated chondrocyte apoptosis and ECM degradation, whereas inhibition of miR-328-3p resulted in the opposite effects. Bioinformatics analysis, RNA immunoprecipitation (RIP), and luciferase assays revealed that AC008 functioned as a competing endogenous RNA (ceRNA) to regulate miR-328-3p, which specifically targeted the AQP1 and ANKH genes. In addition, miR-328-3p significantly ameliorated MIA-induced OA, whereas AC008 accelerated OA progression in vivo. Furthermore, fat mass and obesity-associated (FTO)-mediated N6-methyladenosine demethylation downregulated AC008 transcription, while lower FTO expression led to upregulation of AC008 transcription in OA. In conclusion, our data reveal that AC008 plays a critical role in OA pathogenesis via the miR-328-3p‒AQP1/ANKH pathway, suggesting that AC008 may be a potential therapeutic target for OA.